Abstract
Adolescence is a time period in development when the brain undergoes substantial remodeling in response to the environment. To determine whether a stressful experience during adolescence affects adult behavior, we exposed adolescent male and female C57BL/6J mice to chronic unpredictable stress (CUS) for 12 days starting at postnatal day 28 (PND28). We also exposed adult male and female mice to CUS for 12 days beginning at PND70 to determine whether adolescence is a sensitive time period when stress can have long-lasting effects on behavior. Regardless of when mice were exposed to stress, they were all tested exactly 30 days later in the marble burying task, elevated zero maze, acoustic startle response, and forced swim test. Adolescent stress exposure increased anxiety-like behaviors in adult male and female mice and decreased acoustic startle response in a sex-dependent manner. However, adult stress exposure did not change anxiety or response to an acoustic tone in adult male or female mice as compared with nonstressed animals. Of interest, increased depression-like behavior in the forced swim test was observed in all mice, regardless of when the stress occurred. Gene expression analysis showed significant upregulation of corticotropin releasing factor receptor 2 (CrfR2) in the amygdala of males subjected to CUS during adolescence, but not in males that experienced CUS during adulthood. In contrast, females, regardless of when they were exposed to CUS, were not affected. These data support clinical evidence suggesting that early-life stress may predispose individuals to increased anxiety and depression later in life.
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INTRODUCTION
Adolescence is a dynamic time period for growth and development. The developing nervous system in particular undergoes heightened remodeling in response to the environment (Spear, 2000). During this time, synapse overproduction and pruning (Teicher et al, 1995), as well as transient changes in neurotransmitter and receptor production, facilitate neuronal maturation (Whitaker-Azmitia, 1991). However, extreme early-life adversity may interrupt some of these processes, predisposing an individual to neuropsychiatric disorders and maladaptative behavior later in life (Andersen and Teicher, 2004). The onset of anxiety, depression, and posttraumatic stress disorders have been correlated with exposure to stressors during adolescence (Pelcovitz et al, 1994; Turner and Lloyd, 2004). Specifically, exposure to uncontrollable adverse events throughout childhood is linked to future psychiatric disorders and adolescent stressors are additive in predicting development and persistence of psychiatric events later in life (Kessler et al, 1997). Thus, clinical data suggest that early-life stress that is chronic and uncontrollable constitutes a major risk factor for the development of mental disorders.
A key brain region that mediates central stress response, the amygdala, undergoes significant remodeling through synapse overproduction and pruning during adolescence and is particularly sensitive to early-life stress exposure (Andersen and Teicher, 2004). Maladaptation of stress signaling, specifically altered expression of corticotropin releasing factor (Crf) has been found in the amygdala of rodents exposed to chronic adolescent stress (Plotsky et al, 2005) and CRF signaling in the limbic system mediates control of anxiety (Wiersma et al, 1995), stress (Bale et al, 2002), and startle response (Dirks et al, 2002). In addition to the amygdala, the bed nucleus of the stria terminalis (BNST) mediates response to stressful events via a vast network of projections throughout the central nervous system. Importantly, the BNST has been implicated in sustained stress response and anxiety through CRF-mediated interactions with the amygdala (Davis et al, 2010) and independently mediates startle response (Walker and Davis, 1997).
Animal studies modeling the effects of adolescent stress on behavior later in life suggest that exposure to early-life adversity facilitates differential responses to stressful situations during adulthood, such as enhanced anxiety and stress sensitivity. Chronic exposure to physical, social, or a combination of physical and social stressors during juvenile and prepubescent phases of development results in altered cognition, anxiety, and depression in adulthood in mice (Schmidt et al, 2010; Weiss et al, 2011; Saavedra-Rodríguez and Feig, 2013) and rats (Tsoory et al, 2007; McCormick et al, 2008; Eiland et al, 2012). Although these studies provide evidence for the long-lasting impact of adolescent stress, few studies have conducted a direct comparison of the effects of stress on behavior and neurobiology when experienced by the adolescent vs the adult.
We determined the long-term effects of chronic stress on anxiety, startle response, and depression-like behavior in animals using a chronic unpredictable stress (CUS) paradigm. This paradigm is composed of both physical and social stressors that last 12 days and produces anhedonia and depression phenotypes in mice (Schmidt and Duman, 2010). The use of CUS in rodents mirrors uncontrolled stress experienced by individuals (Hollis et al, 2013) and prevents habituation to stress exposure, thus avoiding attenuated responses (Girotti et al, 2006). Mice were exposed to the same CUS during different developmental time windows, adolescence before puberty (postnatal day (PND) 28–40) or adulthood (PND70–82), and tested during adulthood (30 days following the last day of the stressor). By isolating the time period of stress exposure, we identified adolescence as a sensitive window for CUS to have long-term effects on behavior and gene expression.
MATERIALS AND METHODS
Animals
Male and Female C57BL/6JTac mice (6–8 weeks of age, 20–30 g) were ordered from Taconic Farms (Hudson, NY), group housed, and maintained on a 12 h light/dark cycle with food and water ad libitum in accordance with the University of Pennsylvania Animal Care and Use Committee (Philadelphia, PA). Mice were bred for two generations to generate offspring used in the current study. Breeding within the facility decreased the impact of transportation stress on the mice and allowed us to isolate the effects of the exposure of CUS in adolescents and adults. All experimental testing sessions were conducted between 0800 h and 1700 h, with animals randomly assigned to treatment conditions. Because litters were born at varying times throughout the course of these studies, three different cohorts of mice were used for adolescent CUS exposure, whereas one cohort of mice was used for adult exposure. Separate cohorts of mice were used to determine changes in gene expression following stress exposures.
Chronic Unpredictable Stress
Mice underwent CUS for 12 days during the light phase of the 24 h period starting at PND28 or PND70 (4 weeks or 10 weeks of age). The CUS paradigm was adapted from previous studies (Schmidt and Duman, 2010). The exact stressors can be found in Supplementary Table S1. Briefly, animals were exposed to three stressors each day, in the morning, afternoon, and overnight, for 12 consecutive days in dedicated procedure rooms. Mice were returned to the animal colony between stressors and after the final stressor. Three cohorts of adolescent and one cohort of adult mice underwent CUS, sucrose preference testing, and behavioral testing. To determine whether sex is a major contributor in the efficacy of the CUS to induce anhedonia, we exposed an additional cohort of mice to CUS and evaluated sucrose preference.
Sucrose Preference Test (SPT)
Sucrose consumption was evaluated on the last 2 days of the CUS exposure to determine the consequence of these stressors on sucrose preference in the animals. Mice were habituated to a 1% sucrose solution for 48 h starting on day 6 of CUS exposure to prevent neophobia during testing. Following sucrose exposure, increasing water restriction was used to habituate animals to water restriction: water was restricted for 4 h on day 8, 14 h on day 9, and 19 h on day 10. On day 11, mice were allowed access to sucrose solution in a cage filled with home cage bedding for 1 h without the presence of cage mates. Testing was repeated on day 12 of CUS exposure except that mice were given access to water instead of the sucrose solution. Sucrose preference was reported as the difference between total sucrose consumption divided by total liquid consumption (ml) on both test days.
Behavioral Tests
Behavioral testing was conducted 30 days following the last day of CUS exposure during the light phase of the 24 h period: PND70–82 (10–12 weeks of age) for the adolescent stress exposure group or PND112–126 (16–18 weeks of age) for the adult stress exposure group (Figure 1a). Behavior was assayed in the following sequential order in every animal: (1) marble burying (MB) task, (2) elevated zero maze (EZM), (3) acoustic startle response (ASR), and (4) forced swim test (FST), with a period of rest of at least 2 days between each test. Previous studies demonstrate that order of testing affects behavioral measurements. Therefore, all animals were tested in the same order, and FST was the final behavioral assay to be administered (Wahlsten, 2010). Immediately following the FST, animals were killed and whole brains were rapidly removed and flash frozen in isopentane (−80 °C).
Marble Burying
Differences in anxiety between treatment groups were evaluated using the MB test (Jimenez-Gomez et al, 2011). After a 1 h period of acclimation, mice were placed individually in a test cage that resembled their home cage (26 × 20 × 14 cm). Twenty marbles were distributed evenly in the cages in 5 rows of 4 on top of mouse bedding (5 cm in depth), and a clear lid was placed on top of the cage. Animals were left undisturbed for 15 min, after which time the number of marbles buried, distinguished by being three-fourths or more submerged under bedding, was quantified by a trained observer blind to experimental groups.
Elevated Zero Maze
The EZM was used as a second test of anxiety. Following a 1 h period of acclimation to the testing room, mice were placed in the maze consisting of two open arms and two closed arms elevated 24 inches off the ground and left undisturbed for 5 min. Mice were video recorded for the duration of testing. Time spent in the open arms of the maze was measured by a trained observer blind to experimental groups.
Acoustic Startle Response
The reflexive response to an unexpected tone was assessed using the ASR (Davis, 1980). After a 1 h period of acclimation to the testing room, animals were placed in acoustic startle chambers (SR-Labs, San Diego, CA) for behavioral testing. The chamber consisted of a light- and sound-attenuating outer plastic box and an inner nonrestrictive plastic cylinder chamber affixed to a stage platform. Broadband acoustic startle tones were emitted from a high-frequency speaker mounted above the mouse chamber. Startle reflexes were measured by a piezo electronics monitor mounted under the stage platform. Each testing session lasted 30 min. Animals were habituated to the inside of the startle chamber with a 67 dB sound pressure level (SPL) background white noise for 5 min. After the habituation period, animals were presented with 10 rounds of 5 pseudorandom startle tones (50 total trials) differing in dB SPL (75, 80, 85, 90, 95, 100 105, 100, 115, and 120 dB SPL). Pseudo random interstimulus intervals (ISIs) were generated by the Startle Response software (SR-Labs). ISIs consisted of 26, 28, 30, 32, and 34 s. Immediately after each startle tone presentation, the startle amplitude was measured as the average voltage emitted by the piezo electric pickup each millisecond for the 100 ms response window.
Forced Swim Test
Immobility differences between treatment groups were evaluated using the FST. Mice were placed into plexiglas cylinders filled with water (25 °C; 30–38 cm high) for 6 min, and behavior was video recorded. The time spent immobile during the swim session was recorded by an observer blinded to treatment groups. A mouse was considered immobile when making only those movements necessary to keep its head above water.
RNA Extraction, cDNA Synthesis, and Quantitative Real-Time PCR
Coronal brain sections (300 μm) between Bregma −0.58 and −1.70 for the amygdala, 0.26 and −0.34 for the BNST, and 2.96 and 1.94 for the prefrontal cortex (PFC) (Franklin and Paxinos, 1997) were used to collect 1.2 mm punches bilaterally of each brain region. RNA was extracted by homogenizing in 800 μl of TRIzol and 160 μl of chloroform. RNA was purified using an RNeasy Mini Kit (Qiagen, Cat. No. 74104). RNA concentration and integrity were determined with a Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE). cDNA was synthesized from RNA (100 ng) using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative real-time PCR (qRT-PCR) was carried out using the SYBR-green master mix (Applied Biosystems) and 10 μM primers (final concentration) for corticotropin relating factor (Crf) and corticotropin releasing factor receptors 1 and 2 (CrfR1, CrfR2) on the Stratagen MX3000 using MXPro QPCR software. Primer sequences for Crf, CrfR1, CrfR2, and the housekeepers Tbp and Hprt can be found in Supplementary Table S2. Cycling parameters were 95 °C for 10 min and then 40 cycles of 95 °C (30 s) and 60 °C (1 min), followed by a melting curve analysis. All reactions were run in triplicate, and median cycles to threshold (Ct) values were used for analysis. Housekeeping genes were used to normalize against experimental genes, and relative gene expression was determined using the 2−ΔΔCT method (Livak and Schmittgen, 2001).
Statistical Analysis
All data are presented as mean±SEM. For comparisons of stress effects among both sexes in each behavior (eg, SPT, MB, EZM, FST), a two-way analysis of variance (ANOVA) was used. For comparisons between multiple groups at multiple measurement points (eg, ASR), a repeated measures two-way ANOVA (RM-ANOVA) was used to determine significant differences with tone representing the within, repeated-measures independent factor and stress exposure the dependent variable with sexes analyzed independently. Finally, for comparisons between experimental groups with age and stress as independent variables (eg, mRNA fold change), a two-way ANOVA was used. Statistical analyses were performed using Graphpad Prism 7 (Graphpad Software, La Jolla, CA), with the threshold for statistical significance set as P<0.05, and Bonferroni multiple comparison test used for all post hoc analysis.
RESULTS
Loss of Sucrose Preference Immediately Following CUS Exposure in Adolescents and Adults
Mice exposed to CUS had a significantly lower preference score in the sucrose preference test when tested on the last day of stress exposure as compared with within-group control animals not exposed to adolescent or adult stress (Figure 1b; F1, 37=16.35, P<0.001, Figure 1c; F1, 38=0.3801, P<0.05). In addition, a two-way ANOVA revealed a significant effect of age of testing. Animals exposed to CUS in adulthood (PND82) had a significantly lower preference score compared with animals exposed to CUS during adolescence (PND40) (F1, 54=8.788, P<0.01).
Adolescent CUS Increases Anxiety-Like Behaviors in Adulthood
Exposure to CUS during adolescence increased the number of marbles buried in the MB task by adult mice compared with nonstressed controls (Figure 2a; F1, 48=12.41, P=0.0009). However, mice exposed to CUS during adulthood and tested 30 days later did not bury more marbles than nonstressed controls (Figure 2b; F1, 40=3.622, P=0.0642). In a second test of anxiety, the EZM, adult mice exposed to adolescent CUS spent less time in the open arm of the maze compared with nonstress controls tested at the same time and age (Figure 2c; F1, 33=4.707, P<0.05). A two-way ANOVA also revealed that females, regardless of stress exposure, spent less time in the open arms of the elevated zero maze when tested in adulthood (10 weeks) compared with males (Figure 2c; F1, 33=7.541, P<0.01). In contrast, CUS exposure during adulthood had no effect on anxiety behavior in the EZM in male or female mice (Figure 2d; F1, 39=0.01236, P=0.9120) and no differences were found between sexes.
Adolescent CUS Decreases Acoustic Startle Response at High Decibel Tones in a Sex-Dependent Manner
Exposure to adolescent CUS decreased average startle response at high dB tones in male mice. A two-way RM-ANOVA revealed a main effect of tone (F3, 69=46.72, P<0.0001) and a main effect of adolescent stress exposure (F1, 23=6.573, P=0.0174) along with a tone by stress exposure interaction (F3, 69=5.332, P=0.0023) in male mice on the ASR task (Figure 3a). Multiple comparisons further revealed a significant effect of adolescent stress on startle response at the highest tone administered, 120 dB SPL (P<0.0001). In contrast, CUS exposure during adulthood showed no significant change in startle response at high dB tones when compared with nonstressed controls in males (Figure 3a; F1, 21=0.3038, P=0.5873). However, as expected, there was a main effect of startle tone on startle response amplitude (F3, 63=55.59, P<0.0001).
There was no change in startle response because of adolescent CUS exposure in female mice tested for startle response in adulthood (F1, 25=3.424, P=0.0761), but, as expected, there was a main effect of tone across both treatment groups (Figure 3b; F3, 75=31.17, P<0.0001). Furthermore, female mice exposed to adult CUS showed no significant changes in startle responses compared with nonstressed controls (Figure 3b; F1, 19=2.491, P=0.1310), but did show an expected effect of tone on startle amplitude (F3, 57=37.31, P<0.0001).
Adolescent and Adult CUS Increases Time Spent Immobile in the FST
Exposure to CUS increased total time spent immobile in the FST and decreased latency to the first bout of immobility in both male and female mice, regardless of when stress was presented. A two-way ANOVA revealed that male and female mice exposed to adolescent CUS and tested 30 days later have a trend toward a shorter latency to the first bout of immobility (Figure 4a; F1, 39=3.765; P=0.0596). In addition, there is significant increase in latency to the first bout of immobility in female mice regardless of adolescent stress exposure compared with male mice tested at the same age (12 weeks; F1, 39=4.317, P<0.05). In addition, both male and female mice exposed to adolescent CUS spent significantly more time immobile compared with nonstressed controls (Figure 4b; F1, 39=4.257, P<0.05) with an additional main effect of sex driven by less time spent immobile by control females compared with males (F1, 39=12.7, P<0.01). Male and female mice exposed to CUS during adulthood and tested later in adulthood (18 weeks) showed a decreased latency to the first bout of immobility (Figure 4c; F1, 40=23.04, P<0.0001) and spent more time immobile (Figure 4d; F1, 40=25.7, P<0.0001) compared with controls, but there was no effect of sex. It should be noted that there was a significant effect of age in the no stress group on time spent immobile; female controls tested in the forced swim at 18 weeks spent more time immobile compared with females tested at 12 weeks of age (t14=3.179, P<0.01).
Adolescent CUS Increases CrfR2 in Adult Male Mice
To evaluate molecular changes associated with anxiety and startle reaction, we examined gene expression in the amygdala, BNST, and prefrontal cortex of the adult mice. Crf expression was unaltered in the amygdala, BNST, or prefrontal cortex of male or female animals exposed to either adolescent or adult CUS (Figure 5a). In addition, CrfR1 mRNA levels were not altered by stress in male or female mice in any brain region (Figure 5b). However, it should be noted that there was a main effect of age on the expression of CrfR1 in adult female mice in the amygdala (F1, 45=4.203, P<0.05). Of interest, adolescent CUS exposure but not adult exposure increased expression of CrfR2 mRNA in the amygdala of adult males (main effect of age: F1, 44=7.048, P<0.05 and interaction: F1, 44=12.37, P<0.001; Figure 5c), whereas the same was not found in other brain regions examined or in female mice.
DISCUSSION
Early-life stress may predispose individuals to neuropsychiatric disorders later in life. Childhood trauma is associated with the emergence of stress-related pathologies in adulthood that include depression, anxiety, and posttraumatic stress disorder (Moffitt et al, 2007). In addition, quality of life and coping psychopathology is heavily influenced by experiences during adolescence (Neigh et al, 2009).
We investigated whether or not adolescence represents a window of development that is sensitive to stress exposure. Previous work has identified effects of adolescent stress exposure on adult behavior in mice (Saavedra-Rodríguez and Feig, 2013; Weiss et al, 2011). However, previous studies did not directly compare the effects of the same type of stressors, in both duration and intensity, during adolescence and adulthood. Furthermore, we tested for changes in behavior exactly 30 days following the final day of stress exposure, Thus, in this experimental paradigm we controlled for the type of stress experienced as well as the incubation period between stress and behavior testing. In addition, we used a stress paradigm composed of physical and emotional stressors and one that is both chronic and unpredictable in nature to better model the human experience of stress (Hill et al, 2012).
Approximately 30 days following the end of CUS exposure, stress-exposed male and female mice spent more time immobile in the FST. Conflicting studies report increases and decreases in immobility (depression and antidepressant-like behaviors, respectively) when animals experience stress in adolescence or adulthood (for review see Hollis et al, 2013). Our data demonstrate that with CUS a consistent depression-like phenotype persists in male and female adults regardless of when the stress was administered, in adolescence or adulthood. Although age did not play a role in performance on this task for males, we did find an age difference (12 vs 18 weeks) in time spent immobile between controls in female mice. Thus, the difference between the stress and no stress group may not reflect an increase in mobility in the stressed females but instead a decrease in mobility due to age. As stress exposure still increased immobility in female mice compared with their age-matched controls at 18 weeks, it is likely this is a real effect of prior stress exposure. Although the depression phenotype was affected in all animals regardless of time of stress exposure, we note that this is in contrast to CUS affects on anxiety behaviors that are affected only if stress occurs during adolescence. It is unclear as to why CUS affected depression-like behavior in both ages, but affected more anxiety-related measures when occurring in adolescents. This difference may be because of sensitivity to stress in developing brain areas associated with these distinct behavioral domains (Eiland et al, 2012).
Measurement of anxiety-like behavior in rodents comes with peculiarities and limitations, and no one test provides the ideal model of anxiety (Steimer, 2011). We chose two paradigms that measure different aspects of anxiety. The MB test measures active and compulsive anxiety behavior (Jimenez-Gomez et al, 2011), whereas the EZM measures exploratory behavior in a circular maze (Shepherd et al, 1994). Adolescent CUS exposure increased anxiety-like behavior in both of these tests. Of importance for clinical translation, a predisposition toward an anxious temperament promotes depression and anxiety diagnosis in patients (Nyman et al, 2011). In contrast, adult CUS-exposed mice showed no change in anxiety behavior. Of note, control and CUS-exposed female mice showed greater anxiety behavior in the EZM compared with males. This sex-specific effect has been previously characterized (Dalla et al, 2010) and appears to be a product of age (Walf et al, 2009) and estrous cycle (Gouveia et al, 2004).
To characterize additional stress-relevant behaviors following CUS, we examined startle response to an acoustic tone. We found adolescent CUS decreased startle amplitudes in males, but not females. Because of hormonal differences, males and females are predisposed to experience the effects of stress differently. In fact, programming of limbic stress circuitry and sex-specific responses to stress later in life occurs before puberty and is dependent on intricate sex-specific hormone signaling (Bale et al, 2010). Some studies found female rodents to be resilient to the effects of stress, unless ovariectomized (Galea et al, 1997), whereas others characterized females to be more vulnerable to the effects of stress (Mueller and Bale, 2008). Further research into sex-dependent effects of chronic stress will continue to promote a better understanding of stress-dependent changes in behavior.
Increased anxiety- and sex-specific effects in startle response following adolescent but not adult stress may be related to the vulnerability of the developing extrahypothalamic stress pathway in the amygdala and extended amygdala (BNST) during adolescence (Lupien et al, 2009). Indeed, anxiety and startle response behaviors, as opposed to depression, were particularly sensitive to adolescent stress, possibly because of ongoing amygdalar development in adolescence (Koss et al, 2014). In the amygdala, CRF mediates extrahypothalamic stress response and is implicated in both anxiety and depression disorders. Increased CRF activity in the amygdala is found immediately following chronic stress (Menzaghi et al, 1993), and increases in CRF signaling in the amygdala increases anxiety in rats (Rainnie et al, 2004).
Crf expression and signaling has also been implicated in ASR behavior in rodents. CRF projections from the amygdala to the pontine reticular nucleus mediate startle response in rodents (Davis, 1980), and stimulation of the amygdala (Koch and Ebert, 1993) or pharmacologic increase of CRF signaling enhances startle amplitudes Thus, our findings that CrfR2 was increased in the amygdala suggests that stress exposure may mediate prolonged changes in CRF signaling in the central nervous system. These changes in gene expression occur only when stress is presented during adolescence as no comparable differences were observed in mice that were exposed to CUS during adulthood.
CRFR2 is found predominantly in the medial and posterior nuclei (Chalmers et al, 1995) and is distributed both pre- and postsynaptically in the amygdala (Treweek et al, 2009). Activation of CRFR2, by either CRF or urocortin, mediates stress coping through dampening stress sensitivity (Bale et al, 2002). Therefore, increased CrfR2 expression in the amygdala may contribute to blunted startle response as well as heightened anxiety found in male mice exposed to adolescent stress, the latter supported by evidence that CRFR2 activation is anxiogenic (Reul, 2002). However, future studies need to correlate expression levels with receptor activation. It should be noted that these molecular changes were only found in males exposed to adolescent stress, and increased anxiety in females may be mediated through a different mechanism not explored in these studies.
In summary, evidence from these studies suggest that adolescence is a sensitive window for stress exposure, producing a disposition toward increased anxiety, depression, and altered reactivity to stress in the future. In addition, this characterization extends to both sexes and identified sex-specific differences in response to stress. Together, these data provide evidence that sex and timing of stress exposure are important variables that affect future adult behaviors. Although we show changes in gene expression for members of the CRF signaling pathway, future studies aimed at investigating causal relationships between these proteins and behavior affected by early stress exposure are warranted.
FUNDING AND DISCLOSURE
The authors declare no conflict of interest.
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This work was supported by grants from the National Institute on Drug Abuse (R01DA033646 (to JAB) and T32DA28874 (to NLY)).
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Yohn, N., Blendy, J. Adolescent Chronic Unpredictable Stress Exposure Is a Sensitive Window for Long-Term Changes in Adult Behavior in Mice. Neuropsychopharmacol 42, 1670–1678 (2017). https://doi.org/10.1038/npp.2017.11
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DOI: https://doi.org/10.1038/npp.2017.11
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