Abstract
Mg-Li based alloys hold much attention as potential biomedical materials due to their excellent ductility. A reduced mechanical strength and concern for biocompatibility are exhibited for Mg-Li binary alloys due to the presence of Li element. Addition of the Ca element into Mg-Li alloys leads to an improvement in mechanical strength and biocompatibility. In the present work, the microstructure, mechanical property and corrosion behaviors of three kinds (α, α+β, β) of as-extruded Mg-Li (1, 9 and 15 wt.%)-1Ca alloys were investigated using optical microscope, X-ray diffraction (XRD), tensile, immersion and electrochemical polarization measurements. In vitro biocompatibility was evaluated by cytotoxicity assays, hemolysis and four coagluation tests. The results indicated that the Mg-1Li-1Ca and Mg-15Li-1Ca alloys were characterized by α-Mg and β-Li phases besides Mg2Ca particles, respectively; while the Mg-9Li-1Ca by dual (α-Mg+β-Li) phase together with Mg2Ca phase. The Mg-1Li-1Ca alloy had the highest ultimate tensile strength (UTS) and yield strength (YS) and the lowest elongation (EL) to failure (10.1±1.24%) as well. The EL for the Mg-9Li-1Ca alloy was the highest (52.2±0.01%). The long-term immersion tests revealed a decrease in corrosion resistance with increasing Li content. The results of cytotoxicity assays clearly showed that the Mg-Li-Ca alloys demonstrated no toxicity to L-929 cells in 10% concentration of extracts. The Mg-1Li-1Ca alloy also exhibited an acceptable hemolysis ratio. The results of four coagulation tests designated no sign of thrombogenicity for the Mg-Li-Ca alloys except for the Mg-15Li-1Ca alloy.
摘要
基于良好的延展性, Mg-Li基合金作为潜在的生物材料, 引起了大家的广泛注意. 由于锂元素的存在, Mg-Li二元合金表现出较低的机械强度与人们对其生物相容性的担忧. 而在Mg-Li 合金中添加钙元素则有利于提高其机械强度与生物相容性. 本文中, 通过光学显微镜、 X射线衍射、 拉力拉伸、 浸泡实验以及电化学极化测试等综合评估了三种类型(α, α+β, β) 的挤压态Mg-Li (1, 9 以及15 wt.%)-1Ca 合金的微观结构、 力学性能以及腐蚀行为. 体外生物相容性通过细胞毒性、 溶血以及凝血四项表征. 结果表明, Mg-1Li-1Ca 和 Mg-15Li-1Ca 合金分别由 α-Mg、 β-Li 以及 Mg2Ca 相构成; 而 Mg-9Li-1Ca 则由 α-Mg、 β-Li双相以及 Mg2Ca 相构成. Mg-1Li-1Ca 合金表现出最高的最大抗拉强度和屈服强度, 但其延伸率最低, 仅为10.1±1.24%; Mg-9Li-1Ca 合金的延伸率最高, 为52.2±0.01%. 长时间浸泡实验表明, 随着锂元素含量的增加, 合金的耐蚀性逐渐降低. 毒性试验结果表明, 在10%浸提液中 Mg-Li-Ca 合金对 L-929 细胞无明显毒性反应. Mg-1Li-1Ca 表现出人体可以接受的溶血率. 凝血四项实验结果指出, 除Mg-15Li-1Ca 外, Mg-1Li-1Ca 和 Mg-9Li-1Ca 合金无促凝性反应产生.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (51571134), the Scientific Research Foundation of Shandong University of Science and Technology (SDUST) for Recruited Talents (2013RCJJ006), SDUST Research Fund (2014TDJH104), and Joint Innovative Center for Safe and Effective Mining Technology and Equipment of Coal Resources. Thanks also go to Prof. Rongshi Chen at the Institute of Metals Research, Chinese Academy of Sciences for his preparation for the alloys.
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Lanyue Cui is currently a PhD student at the College of Materials Science and Engineering, Shandong University of Science and Technology. She was born in Zibo, Shandong province, China in 1991. She received her Bachelor’s degree from Shandong University of Science and Technology in 2014. Her research interests are focused on the corrosion and protection of magnesium alloys.
Rongchang Zeng is currently a professor at the College of Materials Science and Engineering, Shandong University of Science and Technology. He received his PhD degree in materials science from the Institute of Metal Research, Chinese Academy of Sciences in 2003. From March 2006 to March 2007, he was a visiting scientist in the Institute of Materials Research of HZG (GKSS). His research focuses on the corrosion and protection of metals and degradable biomedical magnesium alloys.
Yufeng Zheng received his PhD in materials science from Harbin Institute of Technology, China in 1998. Since 2004, he has been a full professor at Peking University in Beijing, China. His research focuses on the development of various new biomedical metallic materials (biodegradable Mg, Fe and Zn based alloys, β-Ti alloys with low elastic modulus, bulk metallic glass, ultra-fine grained metallic materials, etc.).
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Cui, L., Sun, L., Zeng, R. et al. In vitro degradation and biocompatibility of Mg-Li-Ca alloys—the influence of Li content. Sci. China Mater. 61, 607–618 (2018). https://doi.org/10.1007/s40843-017-9071-y
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DOI: https://doi.org/10.1007/s40843-017-9071-y