Abstract
Nutrients in the substrate are important for plant growth, particularly during the development of vegetable seedlings. The continued development of intensive methods to produce seedlings has made growing healthy and high-grade seedlings a requirement for nurseries. This study evaluated the effect of nitrogen (N), phosphorus (P), and potassium (K) on the root activity, dry weight, and chlorophyll content in leaves of Brassica oleracea seedlings. D-optimal design was used in this study because of its accuracy. A total of 10 treatments (T1–T10) were designed, and different concentrations were chosen in the following ranges: N (0.6-5.0 g·kg-1), P (0.2-6.0 g·kg-1), and K (0.7-7.0 g·kg-1). Our study shows that the application of N, P, and K to the substrate significantly influenced the growth of B. oleracea seedlings. T6 significantly increased root activity, root dry weight, chlorophyll content in leaves when compared with the control T1, which had added nutrient concentrations at normal levels. However, the addition of high levels of N (5.0 g·kg-1) with high (7.0 g·kg-1) or low (0.7 g·kg-1) levels of K (T2 and T9) not only significantly decreased the root dry weight but also decreased leaf area. Our results suggest that N, P, and K can be added to the substrate at an appropriate ratio to grow vigorous B. oleracea seedlings under intensive seedling production conditions. The present results give insight into the nutrient requirements during early growth of B. oleracea seedlings in growth substrate.
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Literature Cited
Akiyama M, Miyashita H, Kise H, Watanabe T, Miyachi S, Kobayashi M (2001) Detection of chlorophyll d’ and pheophytin a in a chlorophyll d-dominating oxygenic photosynthetic prokaryote Acaryochloris marina. Anal Sci 17: 205–208
Bodea A, Leucuta SE (1997) Optimization of hydrophilic matrix tablets using a D-optimal design. Int J Pharm 153: 247–255
Bray RH, Kurtz L (1945) Determination of total, organic, and available forms of phosphorus in soils. Soil Sci 59: 39–46
Cakmak I (2005) The role of potassium in alleviating detrimental effects of abiotic stresses in plants. J Plant Nutr Soil Sci 168: 521–530
Cakmak I, Hengeler C, Marschner H (1994) Partitioning of shoot and root dry matter and carbohydrates in bean plants suffering from phosphorus, potassium and magnesium deficiency. J Exp Bot 45: 1245–1250
Clemensson Lindell A (1994) Triphenyltetrazolium chloride as an indicator of fine-root vitality and environmental stress in coniferous forest stands: Applications and limitations. Plant Soil 159: 297–300
Cornelissen J, Lavorel S, Garnier E, Diaz S, Buchmann N, Gurvich D, Reich P, Ter Steege H, Morgan H, Van Der Heijden M (2003) A handbook of protocols for standardised and easy measurement of plant functional traits worldwide. Aust J Bot 51, 335–380
Cui XM, Wang XF (2001) Vegetable seedling substrates and their research progress. Tianjin Agr Sci 1: 0–10
De Aguiar PF, Bourguignon B, Khots MS, Massart DL, Phan Than Luu R (1995) D-optimal designs. Chem Intel Lab Syst. 30: 199–210
El-Hagrasy AS, D’Amico F, Drennen JK (2006) A process analytical technology approach to near-infrared process control of pharmaceutical powder blending. Part I: D-optimal design for characterization of powder mixing and preliminary spectral data evaluation. J Pharm Sci 95: 392–406
Eriksson L, Johansson E, Wikström C (1998) Mixture design-design generation, PLS analysis, and model usage. Chem Intel Lab Sys, 43: 1–24
Fageria NK, Baligar VC, Jones CA (2010) Growth and mineral nutrition of field crops, CRC Press, ISBN 9781439816950
Farage PK, McKee IF, Long SP (1998) Does a low nitrogen supply necessarily lead to acclimation of photosynthesis to elevated CO 2 ? Plant Physiol 118: 573–580
Gruda N, Schnitzler W (2004a) Suitability of wood fiber substrate for production of vegetable transplants: I. Physical properties of wood fiber substrates. Sci Hortic 100: 309–322
Gruda N, Schnitzler W (2004b) Suitability of wood fiber substrates for production of vegetable transplants II.: The effect of wood fiber substrates and their volume weights on the growth of tomato transplants. Sci Hortic 100: 333–340
Gurgul E, Herman B (1994) Influence of nitrogen, phosphorus and potassium on chemical composition and activity of some enzymes in celery during its growth. Biol Planta 36: 261–265
Hao X, Papadopoulos A, Dorais M, Ehret D, Turcotte G, Gosselin A (1998) Improving tomato fruit quality by raising the EC of NFT nutrient solutions and calcium spraying: effects on growth, photosynthesis, yield and quality. In: XXV International Horticultural Congress, Part 1: Culture Techniques with Special Emphasis on Environmental Implications. 511: 213–224
Hatzig SV (2015) Breeding for climate change: genetics and physiology of seed vigor, seedling vigor and early drought resistance in winter oilseed rape (Brassica napus L.). Giessener Elektronische Bibliothek http://geb.uni-giessen.de/geb/volltexte/2015/11814
Izquierdo J (2000) Biotechnology can help crop production to feed an increasing world population-positive and negative aspects need to be balanced: a perspective from FAO. In: Plant genetic engineering: towards the third millennium: Proceedings of the International Symposium on Plant Genetic Engineering, Havana, Cuba, 6-10:13–26
Kratky BA, Peterson LA, Krueger AR (1994) Growing cucumbers in beverage cans resting in shallow tanks of aerated and non-aerated nutrient solution. Ame Soc Plastic 101–107
Kuan KB, Othman R, Abdul Rahim K, Shamsuddin ZH (2016) Plant growth-promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilisation of maize under greenhouse conditions. PLoS ONE 11: e0152478
Kumar N (2006) Breeding of horticultural crops: principles and practices, New India Publishing, ISBN:8189422049
Lambers H, Brundrett MC, Raven JA, Hopper SD (2010) Plant mineral nutrition in ancient landscapes: high plant species diversity on infertile soils is linked to functional diversity for nutritional strategies. Plant Soil 334: 11–31
Lamont WJ (2005) Plastics: modifying the microclimate for the production of vegetable crops. HortTechnology 15: 477–481
Lee SH (1982) Vegetable crops growing in China. Sci Hortic 17: 201–209
Lindström A, Nyström C (1987) Seasonal variation in root hardiness of container-grown Scots pine, Norway spruce, and lodgepole pine seedlings. Can J For Res 17: 787–793
Longstreth DJ, Nobel PS (1980) Nutrient Influences on Leaf Photosynthesis. Plant Physiol 65: 541–543
Marschner H, Cakmak I (1989) High light intensity enhances chlorosis and necrosis in leaves of zinc, potassium, and magnesium deficient bean (Phaseolus vulgaris) plants. J Plant Physiol 134: 308–315
Minotta G, Pinzauti S (1996) Effects of light and soil fertility on growth, leaf chlorophyll content and nutrient use efficiency of beech (Fagus sylvatica L.) seedlings. For Ecol Manage 86: 61–71
Narayan R, Narayan S (2014) Precision farming in vegetables. Precision farming: a new approach, Daya Publishing House, 383, ISBN: 9788170358275
Raghothama K (1999) Phosphate acquisition. Ann Rev Plant Biol 50: 665–693
Rakow G (2004) Species origin and economic importance of Brassica, In: Pua, E.-C., Douglas, C.J. (Eds.) Brassica. Springer Berlin Heidelberg, Berlin, Heidelberg, 3–11
Richardson AD, Duigan SP, Berlyn GP (2002) An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytol 153: 185–194
Rozpadek P, Nosek M, Slesak I, Kunicki E, Dziurka M, Miszalski Z (2015) Ozone fumigation increases the abundance of nutrients in Brassica vegetables: broccoli (Brassica oleracea var. italica) and Chinese cabbage (Brassica pekinensis). Eur Food Res Technol 240: 459–462
Rubatzky VE, Yamaguchi M (2012) World vegetables: principles, production, and nutritive values, Springer Science & Business Media
Sterrett SB (2001) Compost as horticultural substrates for vegetable transplant production. Comp Utili Horti Crop Syst 227–240
Watanabe T, Jansen S, Osaki M (2005) The beneficial effect of aluminium and the role of citrate in Al accumulation in Melastoma malabathricum. New Phytol 165: 773–780
Yanyan L, Fupeng S (2011) Effects of different coated controlledrelease urea on soil ammonia volatilization in farmland. Acta Ecol Sinica 31: 7133–7140
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Zhang, J., Wang, Y., Wang, P. et al. Effect of different levels of nitrogen, phosphorus, and potassium on root activity and chlorophyll content in leaves of Brassica oleracea seedlings grown in vegetable nursery substrate. Hortic. Environ. Biotechnol. 58, 5–11 (2017). https://doi.org/10.1007/s13580-017-0177-2
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DOI: https://doi.org/10.1007/s13580-017-0177-2