Abstract
Over the past years, archaeobotanical studies have clarified much of the process of dispersal and adaptation of crops across Asia. However, the development of farming systems that could function in the high-altitude environments of Tibet requires more in-depth consideration. In this article, we present the results of the systematic archaeobotanical investigation at Karuo, a third millennium BC site in eastern Tibet. We argue that millet cultivation was possibly practiced at the site and that it was likely an important aspect of the economy from 2700 to 2100 cal. BC. The role of millet in the cultivation system might have declined after the mid-second millennium BC, during which time wheat—a grain originating in southwest Asia—appeared at the site. In addition to farming, evidence of foraging, hunting, and fishing are present suggesting a diverse subsistence strategy. The diversification of human diets may have contributed to the long-term occupation of the site. Taking a broad regional perspective into account, the diverse spectrum of subsistence strategy engaged by Karuo people provides new insights into the understanding of early lifeways on the Tibetan Plateau.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Archaeological research on the Tibetan Plateau has drawn considerable scholarly attention in recent years (e.g., Aldenderfer 2011; Brantingham et al. 2013; d’Alpoim Guedes and Aldenderfer 2019; Hein 2018; Hung et al. 2014; Lu 2016; Madsen et al. 2017; Meyer et al. 2017; Zhang et al. 2018). Some of the most lively debated topics include the advent and development of agriculture, as well as the contribution of multi-resource subsistence activities to the residence of the Tibetan Plateau (Chen et al. 2015; d’Alpoim Guedes et al. 2013; d’Alpoim Guedes 2015, 2016; d’Alpoim Guedes and Butler 2015; Dong et al. 2015; Lu 2016; Meyer et al. 2017; Zhang et al. 2019). Some recent research focuses on the adaptation of southwest Asian originating crops, notably barley and wheat, to high-altitude environments (above 2500 m.a.s.l.) as old as 1665–1518 cal. BC (a direct wheat date from Karuo: Liu et al. 2016). Representative sites with evidence of wheat and barley remains are found across the Tibetan Plateau, including many sites in the northeastern plateau, Ashaonao in the eastern plateau, Changguogou and Bangga in the southern region, and Dingdong and Kaerdong in the western region (Fu 2001; d’Alpoim Guedes et al. 2013, 2015; Chen et al. 2015; Dong et al. 2015; Liu et al. 2016; Song et al. 2017; Tang et al. 2021).
The Karuo site is the earliest known Neolithic settlement in high-altitude eastern Tibet and is pivotal for our understanding of early farming and subsistence on the plateau. Charred foxtail millet (Setaria italica) grains were first recovered at the site during early excavations in the 1970s (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985). A total of 77 foxtail and broomcorn millet (Panicum miliaceum) grains/fragments were later identified from test trenches excavated in 2002. (d’Alpoim Guedes et al. 2013). These discoveries highlighted the importance of millet in the diets of the Karuo population. The question remains, however, whether these grains were cultivated locally or derived via trade or plant/seed exchange networks, and scholars have suggested both scenarios (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985, P154; Wu et al. 1985; Li 2007; Lu 2016; d’Alpoim Guedes et al. 2013; d’Alpoim Guedes and Butler 2015; d’Alpoim Guedes 2015, 2016). The subsistence of Karuo is of interest given the growing knowledge of the multi-resource economic spectrum implemented across Tibet, particularly in the eastern Plateau, as drawn from recent investigations (e.g., Zhang et al. 2019; d’Alpoim Guedes et al. 2013; Zhao and Chen 2011). In the context of these, further investigation into the systematically collected materials from recent excavations and additional profile sampling (2012 and 2018) at Karuo is timely.
In this paper, we present the flotation results from excavations in 2012 and additional archaeobotanical sampling in 2018. The results demonstrate that intensive on-site archaeobotanical research is essential for understanding the nature of farming and subsistence, in association with stratigraphic sequences, displaying diachronic changes. These results, together with other recent findings from this region, help us to understand the unique dietary economy in eastern Tibet, and contribute to the understanding of early lifeways on the Plateau as a whole.
Karuo site
Karuo (97° 2′ E, 31° 1′ N) is a prehistoric occupation site in Changdu city, eastern Tibet, with an elevation of 3100 masl (Fig. 1a). It is situated on a terrace at the southern foothills of the Zilongla Mountains, in conjunction between the Karuo River (a small tribute of the Lancang River) to the south of the site and the Lancang River to the east of the site. The eponymous modern Karuo village is approximately 1 km to the west. The landscape of this region is characterized by alternating mountains and valleys (Fig. 1b). The terraces on both sides of the Lancang valley are ideal places for human residence, and many modern settlements are indeed situated at those locations. Evaporation rates on the southern slopes of the mountains and in open river valleys are high, resulting in an arid microenvironment. Plants grow more readily on shady slopes and in narrow river valleys (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985). The vegetation in this region is cataloged as the Changdu-Chaya sub-region of evergreen broad-leaved forests in the middle and lower reaches of the Yarlung Zangbo River. At locations below 3400 masl, dry valley thickets grow, dominated by Artemisia veslita, A. roxburghii, Buddleja crispa var. glandulifera, Ceratostigma minus, Ephedra intermedia var. tibetica, Rhamnus tangulicus, and Sophora davidii, among other plants. In addition, Elsholtzia stauntoni is prominent along roadsides and highly disturbed areas (Comprehensive Scientific Expedition of Tibetan Plateau Chinese Academy of Sciences 1988).
The climate is suitable for cultivation, and as elsewhere on the Tibetan Plateau, and barley is the major crop in the present day. Minor crops include wheat, turnips, and rapeseed. The site is in a plateau temperate semi-arid climatic zone, under the influence of summer monsoons, with an annual average temperature of 6–8 °C, and annual precipitation of 500–700 mm. Precipitation is unevenly distributed throughout the year, with 78% of it concentrated in the summer from May to September. As a result, Karuo is wet in the summer, but very dry in spring and winter. Sunlight in this region is abundant. The average annual daytime duration is 2276.5 h. The cumulated temperature above 5 °C (daily everage) and 10 °C are 2715.3 and 2108 degrees, respectively (Comprehensive Scientific Expedition of Tibetan Plateau Chinese Academy of Sciences 1984; Song and Wang 2013). According to pollen diagrams from the site and nearby lakes, the climate of the study region in the mid-Holocene was warmer and wetter than that of today. Annual average temperature and precipitation were 2 to 3 °C and 150–250 mm higher than those of today, during the period between 7450 and 450 cal. BC (Wu et al. 1985; Tang et al. 2004).
Karuo was excavated during the field seasons of 1978–1979, 2002, and 2012 (Fig. 2). Large-scale excavations (1800 m2) were carried out in 1978 and 1979. Twenty-eight house structures were excavated, including semi-subterranean stone structures. A large quantity of artifacts, including ceramic, lithic, and bone objects, were unearthed. Dense deposits of carbonized millet grains were reported in house structures F8 and F22.29 and within one stratigraphic layer (T4L3) (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985; P10, 27, 41). They were later identified as foxtail millet (Wu et al. 1985). Animal remains were mostly identified as wild species, except for thirty-two pig bones (Sus sp.) (Huang and Leng 1985). The excavators believed that the ancient occupants at Karuo practiced millet farming, hunting, and pig hudsbandry (Cultural Relics Management Committee of Tibet Autonomous Region and Department of history of Sichuan university 1985). The chronology of the site was suggested to span 3050–2050 cal. BC, based on radiocarbon dating results from wood charcoals, which were collected from house structures typical of the early and late phases of the site (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985; Tong et al. 1987).
In 2002, five test trenches (230 m2) were excavated by a joint team of archaeologists from Sichuan University and the Cultural Relics Bureau of the Tibet Autonomous Region to investigate Karuo’s subsistence economy. In total, three houses, sixteen ash pits, one road, and one ditch were excavated. Thousands of animal bones and six flotation samples (> 6 L) were collected from stratigraphic layers, ash pits, and other contexts. Both foxtail and broomcorn millet grains were identified; foxtail millet was more abundant than broomcorn millet (d’Alpoim Guedes et al. 2013). Millet grains were directly dated to between 2750 and 2350 cal. BC (d’Alpoim Guedes et al. 2013). All animal remains from the 2002 excavation were identified as wild species, which would have natural habitats at different altitudes, indicating the importance of local hunting (Li 2007; Zhang et al. 2019).
In 2012, a five by 10 m2 unit (referred to as 2012XCKT1) was excavated to investigate the Karuo subsistence further. In total, nine stratigraphic layers, including layers that represent post-occupational periods (L1-L2), cultural deposits (L3-L6), nature sediments (L7-L9), and few ash pits, and house structures were excavated (Figs. 3, 4, and 5). Animal remains, lithics, and ceramics were collected. During this field season, systematic flotation sampling was carried out at Karuo, led by one of the co-authors (X. Liu), and this is the first time that flotation machine is used in archaeology in Tibet. It is worth noting, however, that the features and associated artifacts recovered in 2012 were considerably less dense than what were recovered in the 1970s, which is likely located at the core area of the settlement (i.e., the 1978–1979 excavation area). To explore the core area further, we cleared three profiles in the 1978–1979 excavation area, including T25/T15, T31, and T35 in 2018 (please refer to Fig. 2 for the location of these profiles). Three stratigraphic layers, a two-room house structure (F22.29) were observed on these profiles (Fig. 6), including the post-occupational layer (L1), and occupation layers of the Karuo culture (L2, L3, F22.29). Thousands of millet grains were visually observable in contexts related to house structure F22.29 (Fig. 7). Flotation samples were collected from these contexts.
Materials and methods
Archaeobotanical assemblages from the 2012 and 2018 field seasons are reported in this article. Sampling and flotation methods differed slightly between the two seasons. In 2012, we designed and built a flotation machine locally, modified from that initially developed by Patty Jo Watson in the 1960s (the SMAP type; Fig. 8). The machine is fed by clean pumped groundwater in a nearby factory. Sediment samples were suspended in a 2000-micron mesh to catch the heavy fraction, with the overflow feeding into a 500-micron mesh to catch the light fraction. In this manner, a total of 89 flotation samples (1402 L of sediment) were floated during the 2012 excavation season. Sample sizes vary, with 10 L as the target volume. We sampled a range of identified features, including pit fills (H2-H6) and fills in the house structures (F2K3, F2K5, F2k8, F3), as well as other contexts through the stratigraphic sequence (L3-L9). Only one sample was collected Only one sample was collected from each layer of L7, L8 and L9, respectively. Plant remains recovered in these three samples were very rare, and no crop remains were recovered; therefore, these samples are not included in the discussion section. Sampling was conducted using MOLAS (1994) procedure with modified MOLAS environmental sampling sheets. In 2018, six flotation samples (89 L) were collected from the reopened profiles in the 1978–1979 excavation area. We sampled features within the house structure F22.29 and stratigraphic units L2 and L3. We floated the 2018 samples in the field, using a bucket method described by Crawford (1983) and Zhao (2004). The light fraction was collected using a sieve with 0.25 mm mesh.
Samples were sorted under a low power binocular microscope at Sichuan University. Carbonized seeds and seed fragments were separated from vascular tissues. In some cases, uncharred seeds were also encountered, but these are regarded as modern intrusions and are not considered in the seed counts. For the samples from the 2012 season, systematic sorting and counting were conducted down to 0.25 mm. For the samples from the 2018 season, systematic counting was also conducted down to 0.25 mm, except for the sample from house structure F22.29. Considering the extremely high density of millet grains, we used a weight estimation method to calculate this sample (original data is attached in Supplement Table 1). Identifications were made on the basis of morphological characteristics, and compared against modern comparative reference materials curated at the archaeobotanical laboratory at Sichuan University and various illustrated identification keys (Li 1998; Guo 1995; Guan 2000; Zhang and Guo 1995).
Results
Plant remains
The plant remains recovered from the Karuo site are listed in Tables 1 and 2 (Fig. 9; sample by sample plant assemblages from the 2012 field season are provided in Supplement Table 2). A large quantity of seeds, including seed fragments, was identified. The majority of the seeds in the assemblage were millets or agricultural weeds, e.g., grasses. Wheat was also present, but in very low abundance and only occurred in the later period, after 1665 cal. BC. There are also some seeds that may belong to edible fruits, such as Rubus sp.
Chronology of the site
The excavators of the 1978–1979 field seasons suggested a date range of 3050–2050 cal. BC, based on conventional radiocarbon measurements of wood charcoal speciments from house structures typical of different phases (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985; Tong et al. 1987). Mu (1987), however, questioned this chronological range and pointed that only three radiocarbon dates were referenced in the 1985 report, while 41 radiocarbon measurements were available by the time. Subsequently, after analysis of the 41 radiocarbon results, derived from wood charcoal specimens, Wang (1994) proposed a modified time span of 3350–2350 cal. BC. Shi (1994), on the other hand, argued that the start point of the occupation might be earlier than 3350 cal. BC and the site might be in use until around 2250 cal. BC, taking the stratigraphic relations into account. In addition to these differing opinions, some dates from supposedly different occupational phases are contemporaneous, indicating potential intrusions of recent-age charcoals into earlier layers. Finally, all of the 41 dates were derived from wood charcoal, which has inherent problems, notably in the context of the old-wood effect (d’Alpoim Guedes et al. 2013).
To better understand the chronology of the site and subsistence activities, we submitted five single grain crop specimens (three millet grains and two wheat grains) and four bone specimens selected from the 2012 collection, together with five millet samples (multiple grains for each specimen) from the 2018 collection for AMS dating (Table 3; Fig. 10). Radiocarbon (14C) analyses were conducted at Oxford Radiocarbon Accelerator Unit (ORAU) and through Beta-Analytic. The sample preparations undertaken at these labs were similar, with a standard acid-base-acid (ABA) chemical pre-treatment method. It is worth noting, two single millet grains were analyzed at Oxford (OxA–28025 and OxA–28026) utilizing the methodological refinements for very small sample sizes (small graphite), as described in Motuzaite Matuzeviciute et al. (2013). These were necessary since the weights of single charred millet were such that they fell below the standard graphite yields. Another single millet grain (Beta-560376) from the 2012 collection was analyzed using a similar pretreatment procedure via the service of Beta-Analytic.
The dates of specimens from 2018 ranged roughly between 2500 and 2100 cal. BC, while the dates of specimens from 2012 show a broad range of 2800–700 cal. BC. The 2018 sampling was conducted in the core area (1978–1979 excavation area) of the site, which is now under conservation restrictions. This prevents us from sampling contexts other than 1978–1979 excavation profiles, including layers beneath the initial excavation. We suspect the oldest anthropogenic activities at this “core area” predate 2500 cal. BC. As for the 2012 excavation, 2855–2810 cal. BC is the oldest date available thus far. The 2012 excavation was conducted in the southwestern sector of the site, which may not represent the earliest occupation of the site. It is believed the northern part of the site is older according to the initial report (Cultural Relics Management Committee of Tibet Autonomous Region and Department of History of Sichuan University 1985). Nevertheless, our results suggest that the terminus date is much younger than what we previously considered and the site was in use for a more extended period until the first millennium BC. It is also interesting to note that there is a chronological gap—between 2100 and 1600 cal. BC—in the radiocarbon results. To sum, the new radiocarbon measurements obtained from floral and faunal remains are better indications of subsistence related activities comparing to wood charcoal, illustrating a much longer duration of the site—lasting more than two millennia—with a potential chronological gap for five centuries between 2100 and 1600 cal. BC.
Discussion
Millet
Broomcorn and foxtail millet were first cultivated along a series of foothill locations broadly running northeast-and-southwest, adjacent to the eastern edge of the Loess Plateau and the Mongolian Plateau, around 6000 BC (Liu et al. 2009; Liu et al. 2015). Between 5000 and 2500 BC, millet cultivation became visible in the archaeological record of regions far beyond its center of origin (Liu et al. 2019). For example, broomcorn millet is reported in Primorye of the Russian Far East by 3000 BC (Sergusheva 2006), and foxtail millet reached Taiwan in the late third millennium BC (Tsang 2012). Before and during the third millennium BC, both millets are reported in abundance in Gansu and eastern Qinghai (e.g., Chen et al. 2015; Zhou et al. 2016; Dong et al. 2018). From there, the cultivation of these crops likely moved southward and reached the mountainous region of western Sichuan and the eastern Tibetan Plateau between 3350 and 2650 cal. BC (Zhao and Chen 2011). At Karuo, the macrofossil evidence of both millet species and the direct radiocarbon measurements associated with them is consistent with previously known evidence for the dispersal of millet. By 1500 BC, the geographic expansion of millet cultivation stretched across the Eurasian continent and northeast Africa (e.g., Fuller et al. 2011; Frachetti 2012; Spengler et al. 2014; Stevens et al. 2016; Liu et al. 2018). In Tibet, both species are recorded at Changguogou by the Yarlung Tsangpo in central Tibet, dated to c. 1500 BC (Fu 2001; d’Alpoim Guedes et al. 2013).
An insightful question is whether these millet grains at Karuo were cultivated locally, or they were obtained through a network of exchange and trade (e.g., d’Alpoim Guedes et al. 2016). Both broomcorn and foxtail millet utilize the C4 (Hatch-Slack) photosynthetic pathway. In high altitude environments, C4 photosynthesis is generally considered to be maladapted. The contribution of C4 species to local floras and vegetation stands shows a sharp decline with increasing altitude (see Sage and Monson 1999 for a review). High elevation locations, like Karuo (c. 3100 masl), create considerable challenges for millet cultivation, due to the difficulties in meeting physiological and practical requirements for low-temperature tolerance. Between the two millets, Setaria italica is better at adapting to high altitude environments than Panicum miliaceum (e.g., d’Alpoim Guedes et al. 2013; Sage et al. 2015). This is consistent with our results, showing that there are more foxtail millet grains than broomcorn millet in the Karuo assemblage. Previous studies highlighted the ecological challenge to millet cultivation and hypothesized that populations of Karuo might have potentially become involved in small-scale cultivation of foxtail millet, but broomcorn millet cultivation was less likely, and some of these grains were possibly moved through trade or networks of exchange (d’Alpoim Guedes and Butler 2015; d’Alpoim Guedes 2015; 2016; d’Alpoim Guedes et al. 2016).
It is not uncommon for communities—regardless of subsistence strategy—to trade grain (among other commodities) or exchange seeds for a variety of social and economic reasons, including to maintain the genetic diversity of landraces in high altitude environments (e.g., Frachetti 2014; Asfaw 1999). We consent with the previous suggestions that some millet grains at Karuo were derived from trade or exchange networks (d’Alpoim Guedes and Butler 2015; d’Alpoim Guedes 2015, 2016; d’Alpoim Guedes et al. 2016). It is, nevertheless, helpful to consider the potential of local millet cultivation near Karuo. Here, the presence of Setaria viridis and other Panicoidieae taxa at the site is noteworthy. These taxa are commonly known as arable weeds in agricultural fields in northern China today. Archaeobotanists often encounter them in assemblages where millet is dominating, spanning from the Neolithic to the Bronze Age in the Yellow River region. The presence of Setaria viridis and other Panicoidieae taxa hints at the possible existence of agricultural fields near Karuo. These weedy species were likely incorporated into the archaeobotanical assemblage through harvesting or weeding. On-going morphometric analysis of foxtail millet caryopses from Karuo shows distinct morphological characteristics, indicating that these grains may belong to a unique landrace that is morphologically different from lowland varieties. This finding is consistent with the modern observations of the morphological differences between Tibetan foxtail millet landraces and those from lowland China (Hu 1995: P161-164), and adds to the considerable literature on the variation of C4 landraces (such as maize and finger millet) concerning altitudinal adaption (Goodman and Brown 1988; Tsehaye et al. 2005). Setaria viridis, in particular, is a weedy relative of the domesticated foxtail millet (Setaria italica) and a notable cold-tolerant C4 annual grass, adapting to a range of extreme environments in Asia and America (Sage et al. 2015). It is not impossible that early developments of high-altitude adapted millet landraces were achieved through the crossing between S. italica and S. viridis and that both are present at Karuo. Additionally, many stone sickles have been recovered at the site, indicating grain harvesting (Cultural Relics Management Committee of Tibet Autonomous Region and Department of history of Sichuan university 1985).
In terms of cultivation practices, it is well documented that various cultivation strategies were commonly used in Tibet, including flexibility in sowing time and management of cultivation depth, soil preparation and weeding—to control and modify the thermal, hydrological, and nutrient environments to optimize the growing condition during germination and plant development (Hu ed. 1995: p. 252-255). Should these cultivation strategies be employed with the development of high-altitude-adapted landraces, it would have been possible to cultivate millet near Karuo. However, archaeobotanical reconstructions of these practices are conceptually challenging without additional evidence, and future investigations into the crop processing practice would be profitable (e.g., Song et al. 2013; Liu et al. 2017a). Today, both foxtail and broomcorn millet cultivation is known to communities in a range of regions in southern and eastern Tibetan Plateau, including Changdu, as well as areas near Lhasa and Shigatse, at altitudes as high as 3000 masl (Hu ed. 1995: P161-164). Paleoenvironmental studies demonstrate that the environment was potentially wetter and warmer in the Changdu region during the period of the Karuo occupation further supporting the argument of local cultivation (Liu et al. 1997; Tang et al. 2004).
Millet was present throughout the occupation period of the site from 2800 to 700 cal. BC, inferring a long engagement with millet. Diachronic changes, however, can be observed in the 2012 samples (Fig. 11). The relative frequency and ubiquity both show that there is a decrease in the proportion of millet remains from earlier phase L5 to later L3, which might indicate the decline of millet economy or changing functions of the area in the context of assemblage formation. Our results show millet dates concentrate in a period between 2600 and 2100 cal. BC, which is consistent with the millet dates from the 2002 sampling previously published (d’Alpoim Guedes et al. 2013). It is likely that millet contributed most significantly to local diets during the second half of the third millennium BC.
Wheat and barley
Turning our attention from millet to cereal crops originating in southwest Asia, two charred wheat and one possible barley grain fragment were recovered at Karuo. The two wheat grains were directly dated to the first half of the second millennium BC (Liu et al. 2016; Lu 2016). It is worthy to note that the two wheat dating results—1665–1518 cal. BC and 1207–1017 cal. BC from layers 4 and 3, respectively—are significantly younger than the dated millet grains from the same layers, indicating potential movements between stratigraphic units. Such movement of macrofossil resonates with the “early” dates of broomcorn millet in Europe, indicating intrusions of recent-age grains from the post-Neolithic layers (Motuzaite Matuzeviciute et al. 2013). Nevertheless, wheat did exist at Karuo in the second millennium BC, which suggests that during the late phase of occupation, people at Karuo incorporated novel crops into their diet. There has been considerable discussion on the routes and chronology for the eastern expansion of these Fertile Crescent crops (e.g., Spengler et al. 2014; Liu et al. 2016, 2017b; Stevens et al. 2016; Zhou et al. 2020). We consider wheat and barley to have been introduced to China via multiple pathways—each distinct in time and space—and underlined by different social and culinary drivers (Liu et al. 2016, 2017b; Lister et al. 2018). The dates of the Karuo wheat are consistent with other recent evidence from locations in central and western Tibet, such as Changguogou, Bangga, the new sites in Ngari, and even in Kashmir and Kyrgyzstan. Between 1500 and 1000 cal. BC, naked barley and potentially free-threshing wheat had become common cereals across the west, south, and eastern Tibetan Plateau. Wheat and barley from eastern Qinghai, however, predates this trend by a few centuries (Chen et al. 2015).
Other potential food resources
In addition to millet and cereal grains, a large amount of small wild herbaceous plant seeds were recovered. The list includes Rubus, Artemisia, and Potentilla/Fragaria/Duchesnea, species in the Poaceae family, as well as Chenopodium. Many of these plants may be used by humans as food resources, and a previous study suggested foraging activities at Karuo (d’Alpoim Guedes et al. 2013). Chenopodium is especially notable for constituting the highest proportion of seeds in the floated samples of almost every occupational period at the site (Fig. 12). This evidence should be considered in the context of the assemblage formation process. In Central and West Asia for example, Spengler (2019) highlights that endozoochoric seeds (seed dispersed through animal ingestion) are often overrepresented and could have been preserved through dung burning. In Central and West Asia, the most prominent of such seeds in dung assemblages are from the Amaranthaceae family, notably Chenopodium (Spengler 2019). At Karuo, however, the lack of dung-producing domesticated ruminants, such as sheep/goat and cattle/yak, argues against the usage of dung as fuel. In a nearby Karuo cultural site, Xiaoenda, where zooarchaeological research has been carried out systematically, domesticated ruminants are also absent (Zhang et al. 2019). Wild ungulates do exist at Xiaoenda, so dung could be collected across the landscape. However, neither the surrounding environment of Karuo nor Xiaoenda is the typical landscape for the dung collection. Alternative pathways for the formation of a Chenopodium-rich assemblage at Karuo should be considered.
Some archaeological evidence in north China suggests possible human uses of Chenopodium. For example, at Han Yangling Mausoleum, a large quantity of potentially cultivated Chenopodium giganteum were recovered (Yang et al. 2009). At Erlitou, an urban site in the Xia period, tens of thousands of Chenopodium seeds were recovered from what are suggested to be storage pits. Both contexts hint at human uses, rather than endozoochoric dispersal, and deserve further investigation (Zhao 2014). Large quantities of Chenopodium are also frequently encountered in sites in southwest China, such as at Baiyangcun and Haimenkou in Yunnan, as well as at Shawudu and Yingpanshan in Sichuan (Xue 2010; Martelloa et al. 2018; Zhao and Chen 2011; Yan personal communication). These sites are situated in landscapes with dynamic ecological zones along altitude gradients, similar to the ecological settings of ethnographically documented Chenopodium (and rice and soybean) cultivation in the Himalayan region in India (Pratap and Kapoor 1985). It is highly possible that Chenopodium was utilized by people in the mountainous regions of southwest China as well. Future research into morphological traits resulting from cultivation or selection is necessary.
Subsistence strategy
The presence of millet and cereal grains, and evidence for potential local cultivation, in addition to exchange and gathering activities at Karuo suggests a broad spectrum of subsistence strategy. Fishbones were also retrieved at the site during the 2002 and 2012 excavations, providing evidence for fishing (Li 2007; Zhang 2013). Combining all currently available archaeobotanical and zooarcaheological data at Karuo, its prehistoric inhabitants likely practiced a multi-resource subsistence strategy, which may have included farming, hunting, gathering, and fishing, to fully utilize available resources that enabled long-term occupation of the site. This is similar to the subsistence strategy (millet farming and localized hunting) proposed at Xiaoenda, a recently revealed Karuo cultural site that is 14 km away (Zhang et al. 2019).
In discussing the animal-based subsistence strategies at these sites, Zhang et al. (2019) highlight the dependence on diverse wild animal resources in the local environment around Xiaoenda and Karuo. Zooarchaeological data indicate that the inhabitants took advantage of the local resources situated between highland and multiple lower elevation catchment zones, which allowed access to a range of wild prey species, including musk deer, roe deer, and likely goral and blue sheep. These practices with diverse localized hunting strategies focus on wild rather than domesticated animals are key subsistence strategies at Karuo and Xiaoenda. Together with the archaeobotanical results presented in this paper, Karuo provides a good example (among many others in the world) to challenge the traditional “modes” of subsistence stages—hunting, foraging, pastoralism, and farming—in a linear evolutionary framework. It is evident that people move fairly fluidly between distinctive modes of subsistence, particularly in challenging environments, such as at high altitudes (Scott 2017). In China and elsewhere, it has become clear that early peoples combined subsistence modes in a variety of innovative hybrids (Liu and Reid 2020), and Karuo is contributing to this growing literature.
Conclusion
Systematic archaeobotanical investigation at Karuo improves our understanding of the diverse subsistence strategies at the site, where millet agriculture may have been established as early as 2800 cal. BC. From 2700 to 2100 cal. BC, millet agriculture likely prospered at an elevation of 3100 masl. Wheat occurred at the site starting around the middle of the second millennium BC, indicating multi-grain utility in subsistence. In addition to crop remains, there are other potential food resources that could have been utilized, such as Chenopodium. Combining the results of archaeobotanical and zooarchaeological research at Karuo, we demonstrate the presence of a multi-resource economy at the site, including agriculture, hunting, fishing, and gathering. Our findings at the site, along with evidence from other sites in the region, suggests a diverse subsistence strategy, adding to the literature calling for rethinking of the conventional perception of subsistence modes.
References
Aldenderfer M (2011) Peopling the Tibetan Plateau: insights from archaeology. High Alt Med Biol 12(2):141–147
Asfaw Z (1999) The barleys of Ethiopia. In: Brush SB (ed) Genes in the Field: On-Farm Conservation of Crop Diversity. International Plant Genetic Resources Institute, International Development Research Centre, and Lewis Publishers, Rome, pp 77–108
Brantingham PJ, Xing G, Madsen DB, Rhode D, Perreault C, Woerd J et al (2013) Late occupation of the high-elevation Northern Tibetan Plateau based on cosmogenic, luminescence and radiocarbon ages. Geoarchaeology 28(5):413–431
Chen F, Dong G, Zhang D et al (2015) Agriculture facilitated permanent human occupation of the Tibetan Plateau after 3600 BP. Science 347:248–250
Comprehensive Scientific Expedition of Tibetan Plateau, Chinese Academy of Sciences (1984) Agricultural geography in Tibet. Science press, Beijing (in Chinese)
Comprehensive Scientific Expedition of Tibetan Plateau Chinese Academy of Sciences (1988) Vegetation of Xizang (Tibet). Science press, Beijing (in Chinese)
Crawford GW (1983) Paleoethnobotany of the Kameda peninsula of Jomon. Michigan, Ann Arbor
Cultural Relics Management Committee of Tibet Autonomous Region and Department of history of Sichuan university (1985) Changdu Karuo: a Neolithic site in Tibet. Cultural Relics Publishing House, Beijing (in Chinese)
d’Alpoim Guedes J (2015) Rethinking the spread of agriculture to the Tibetan Plateau. The Holocene 25:1498–1510. https://doi.org/10.1177/0959683615585835
d’Alpoim Guedes J, Aldenderfer M (2019) The archaeology of the early Tibetan Plateau: new research on the initial peopling through the early Bronze Age. J Archaeol Res 28:339–392. https://doi.org/10.1007/s10814-019-09137-6
d’Alpoim Guedes J, Butler E (2015) Comment on “Agriculture facilitated permanent human occupation of the Tibetan Plateau after 3600 BP”. Science 348:872-b
d’Alpoim Guedes J, Lu H, Li Y, Spengler RN, Wu X, Aldenderfer MS (2013) Moving agriculture onto the Tibetan plateau: the archaeobotanical evidence. Archaeol Anthropol Sci 6:255–269. https://doi.org/10.1007/s12520-013-0153-4
d’Alpoim Guedes J, Lu H, Heinc AM, Schmidtd AH (2015) Early evidence for the use of wheat and barley as staple crops on the margins of the Tibetan Plateau. PNAS. 112:5625–5630. https://doi.org/10.1073/pnas.1423708112
d’Alpoim Guedes J, Manning SW, Bocinsky RK (2016) A 5500-year model of changing crop niches on the Tibetan plateau. Curr Anthropol 57:517–522. https://doi.org/10.1086/687255
d'Alpoim Guedes J (2016) Did foragers adopt farming? A perspective from the margins of the Tibetan Plateau. Quat Int 489:91–100. https://doi.org/10.1016/j.quaint.2016.12.010
Dong G, Zhang D, Liu X, Liu F, Chen F, Jones M (2015) Response to “Comment on Agriculture facilitated permanent human occupation of the Tibetan Plateau after 3600 BP”. Science 348:872-c
Dong G, Yang Y, Liu X, Li H, Cui Y, Wang H, Chen G, Dodson J, Chen F (2018) Prehistoric trans-continental cultural exchange in the Hexi Corridor, northwest China. The Holocene 28(4):621–628
Frachetti M (2012) Multiregional emergence of mobile pastoralism and nonuniform institutional complexity across Eurasia. Curr Anthropol 53:2–38
Frachetti M (2014) Seeds for the Soul: ideology and diffusion of domesticated grains across Inner Asia. In: Mair V, Hickman J (eds) Reconfiguring the Silk Road. University of Pennsylvania Museum Press, Philadelphia
Fu D (2001) Identification and research on the Neolithic crop remains at Changguogou site, Tibet. Archaeology 3:66–74 (in Chinese)
Fuller DQ, Boivin N, Hoogervorst T, Allaby R (2011) Across the Indian Ocean: the prehistoric movement of plants and animals. Antiquity 85(328):544–558
Goodman MM, Brown WL (1988) Races of corn. In: Sprague GF, Dudley JW (eds) Corn and corn improvement. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, pp 33–79
Guan G (2000) Illustration of weed identification. Science Press, Beijing (in Chinese)
Guo Q (1995) Color illustration of weed identification. Chinese Agricultural Press, Beijing (in Chinese)
Hein A (2018) Archaeology of the Tibetan plateau. In: Smith C (ed) Encyclopedia of global Archaeology. Springer Nature, Switzerland. https://doi.org/10.1007/978-3-319-51726-1_2880-1
Hu S (1995) An introduction to agriculture in Tibet. Sichuan Science and Technology Press, Chengdu (in Chinese)
Huang W, Leng J (1985) Identification of faunal remains from Karuo site and research on Tibetan plateau climate. In: Cultural Relics Management Committee of Tibet Autonomous Region and Department of history of Sichuan university (ed) Changdu Karuo: a Neolithic site in Tibet. Cultural Relics Publishing House, Beijing, pp 160–166 (in Chinese)
Hung L, Cui J, Chen H (2014) Emergence of Neolithic communities on the northeastern Tibetan Plateau: Evidence from the Zongri cultural sites. In: Hei A (ed) The ‘Cresent-Shaped Cultural-communication Belt’: Tong Enzheng’s Model in retrospect and examination of methodological, theoretical and material concerns of long-distance interactions in East Asia. BAR International Series, Oxford, pp 65–78
Li Y (1998) Chinese weed flora. Chinese Agricultural Press, Beijing (in Chinese)
Li Y (2007) Animal bones and economy at the site of Karuo: an opinion on prehistoric agriculture in the Hengduan Mountain China. Sichuan Cultural Relics 5:50–56 (in Chinese)
Lister DL, Jones H, Oliveira HR, Petrie CA, Liu X, Cockram J, Kneale CJ, Kovaleva O, Jones MK (2018) Barley heads east: genetic analyses reveal routes of spread through diverse Eurasian landscapes. PLoS ONE 13(7):e0196652
Liu X, Reid REB (2020) The prehistoric roots of Chinese cuisines: Mapping staple food systems of China, 6000 BC–220 AD. PLoS ONE 15(11):e0240930. https://doi.org/10.1371/journal.pone.0240930
Liu G, Shi Y, Sheng Y, Hong M (1997) Holocence megathermal environment in the Tibetan Plateau. J Glaciol Geolcryol 19:114–123 (in Chinese)
Liu X, Hunt HV, Jones MK (2009) River valleys and foothills: changing archaeological perceptions of north China's earliest farms. Antiquity 83(319):82–95
Liu X, Fuller DQ, Jones MK (2015) Early agriculture in China. In: Barker G, Goucher C (eds) The Cambridge World History - volume II: a world with agriculture, 12,000 BCE-500 CE. Cambridge University Press, Cambridge, pp 310–334
Liu X, Lister DL, Zhao Z, Staff RA, Jones PJ, Zhou L et al (2016) The virtues of small grain size: potential pathways to a distinguishing feature of Asian wheats. Quat Int 426:107–119
Liu X, Zhao Z, Jones MK (2017a) From people’s commune to household responsibility: Ethnoarchaeological perspectives of millet production in prehistoric northeast China. Archaeol Res Asia 11:51–57
Liu X, Lister DL, Zhao Z, Petrie CA, Zeng X, Jones PJ, Staff R, Pokharia AK, Bates J, Singh RN, Weber SA, Motuzaite Matuzeviviute G, Dong G, Li H, Lü H, Jiang H, Wang J, Ma J, Tian D, Jin G, Zhou L, Wu X, Jones MK (2017b) Journey to the East: diverse routes and variable flowering times for wheat and barley en route to prehistoric China. PLoS ONE 12(11):e0209518
Liu X, Motuzaite Matuzeviciute G, Hunt HV (2018) From a fertile idea to a fertile arc: the origins of broomcorn millet 15 years on. In: Lightfoot E, Liu X, Fuller DQ (eds) Far from the hearth: essays in honour of Martin K. Jones. McDonald Institute Conversations, Cambridge, pp 155–164
Liu X, Jones PJ, Motuzaite Matuzeviviute G, Hunt HV, Lister DL, An T, Przelomska N, Kneale CJ, Zhao Z, Jones MK (2019) From ecological opportunism to multi-cropping: mapping food globalisation in prehistory. Quat Sci Rev 206(15):21–28
Lu H (2016) Colonization of the Tibetan Plateau, permanent settlement and the spread of agriculture: reflection on current debates on the prehistoric archeology of the Tibetan Plateau. Archaeol Res Asia 5:12–15. https://doi.org/10.1016/j.ara.2016.02.010
Madsen DB, Perrreault C, Rhode D, Sun Y, Yi M, Brunson K, Brantingham PJ (2017) Early foraging settlement of the Tibetan plateau highlands. Archaeol Res Asia 11:15–26
Martelloa RD, Min R, Stevens C, Higham C, Highamd T, Qin L, Fuller DQ (2018) Early agriculture at the crossroads of China and Southeast Asia: Archaeobotanical evidence and radiocarbon dates from Baiyangcun, Yunnan. J Archaeol Sci Rep 20:711–721
Meyer M, Aldenderfer M, Wang Z, Hoffmann D, Dahl J, Degering D, Haas W, Schlütz F (2017) Permanent human occupation of the central Tibetan plateau in the early Holocene. Science 355:64–67
MOLAS (1994) Archaeological Site Manual (third edition). Museum of London
Motuzaite Matuzeviciute G, Staff RA, Hunt HV, Liu X, Jones MK (2013) The early chronology of broomcorn millet (Panicum miliaceum) in Europe. Antiquity 87:1073–1085
Mu J (1987) Introduction to Changdu Karuo. Archaeology 1:95–96 (in Chinese)
Pratap T, Kapoor P (1985) The Himalayan grain Chenopods Ι: distribution and ethnobotany. Agric Ecosyst Environ 14:185–199
Sage R, Monson R (1999) C4 plants biology. Academic Press, San Diego
Sage RF, de M Peixoto M, Friesen P, Deen B (2015) C4 bioenergy crops for cool climates, with special emphasis on perennial C4 grasses. J Exp Bot 66(14):4195–4212
Scott JC (2017) Against the Grain: A deep history of the earliest states. Yale University Press, New Haven
Sergusheva EA (2006) Seeds and fruits from late Neolithic site Rettichovka - Geologitcheskaya of Primorye region. In: Hiroki O (ed) Cultivated Cereals in Prehistoric and Ancient Far East Asia 2. Shimoda Print, Kumamoto, pp 1–11
Shi Y (1994) Study on some issues of Karuo remains. In: Research Center for Tibetan Archaeology and History of Sichuan University, Cultural Relics Management Committee of Tibet Autonomous Region (ed) Tibetan Archaeology (Vol. 1). Sichuan University Press, Chengdu, pp 77–90 (in Chinese)
Song S, Wang P (2013) Climate of Tibet. Meteorological Press, Beijing (in Chinese)
Song J, Zhao Z, Fuller DQ (2013) The archaeobotanical significance of immature millet grains: an experimental case study of Chinese millet crop process. Veg Hist Archaeobotany 22(2):141–152
Song J, Lu H, Zhang Z, Liu X (2017) Archaeobotanical remains from the mid-first millennium AD site of Kaerdong in western Tibet. Archaeol Anthropol Sci 10:2015–2026. https://doi.org/10.1007/s12520-017-0521-6
Spengler RN (2019) Dung burning in the archaeobotanical record of West Asia: where are we now? Veg Hist Archaeobotany 28:215–227
Spengler R, Frachetti M, Doumani P, Rouse L, Cerasetti B, Bullion E, Mar’yashev A (2014) Early agriculture and crop transmission among Bronze Age mobile pastoralists of Central Eurasia. Proc R Soc B Biol Sci 281(1783):1–7
Stevens CJ, Murphy C, Roberts R, Lucas L, Silva F, Fuller DQ (2016) Between China and South Asia: a middle Asian corridor of crop dispersal and agricultural innovation in the Bronze Age. The Holocene 26:1541–1555. https://doi.org/10.1177/0959683616650268
Tang L (2018) Early pastoralism on the Middle Branch of Yarlung Zangbo River Region, 1st Millennium BC: the ethnographical and archaeobotanical evidence from Bangga site. Dissertation, Sichuan University (in Chinese)
Tang L, Lu H, Song J, Wangdue S, Chen X, Zhang Z, Liu X, Boivin N, Spengler RN (2021) The transition to a barley-dominant cultivation system in Tibet: First millennium BC archaeobotanical evidence from Bangga. Journal of Anthropological Archaeology 61:101242
Tang L, Shen C, Liao G, Yu S, Li C (2004) Climate change in Southeast Tibetan Plateau since the Last Glacial Maximum. Sci China Ser D Earth Sci 34:7 (in Chinese)
Tong E, Leng J, Wangdui S (1987) Unvraveling Tibetan prehistory at Karuo. Archaeology 35(62-63):81
Tsang Z (2012) Issues relating to the ancient rice and millet grains unearthed from the archaeological sites in Tainan Science Park. J Chin Dietary Cult 8(1):1–24
Tsehaye Y, Berg T, Tsegaye B, Tanto T (2005) Farmers’ management of finger millet (Eleusine coracana L.) diversity in Tigray, Ethiopia and implications for on-farm conservation. Biodivers Conserv 15:4289–4308
Wang R (1994) Several issues on the Qugong culture. In: Research Center for Tibetan Archaeology and History of Sichuan University, Cultural Relics Management Committee of Tibet Autonomous Region (ed) Tibetan Archaeology (Vol. 1). Sichuan University Press, Chengdu, pp 63–75
Wu Y, Yu Q, Kong Z (1985) Analysis of pollen and cultivated crops samples from Karuo Site. In: Tong E, Leng J, Hou S, Wangdui S (eds) Changdu Karuo: a Neolithic Site in Tibet. Cultural Relics Publishing House, Beijing, pp 167–169 (in Chinese)
Xue Y (2010) Study of plant remains from Haimenkou site in Jianchuan County, Yunnan Province. Dissertation, Peking University (in Chinese)
Yang X, Liu C, Zhang J, Yang W, Zhang X, Lu H (2009) Plant crop remains from the outer burial pit of the Han Yangling Mausoleum and their significance to Early Western Han agriculture. Chin Sci Bull 54:1738–1743. https://doi.org/10.1007/s11434-009-0048-z
Zhang Z (2013) Animal remains from the Karuo Site, Tibet. Dissertation, Sichuan University
Zhang Z, Guo Q (1995) Illustration of weed identification. Chinese Agricultural Press, Beijing (in Chinese)
Zhang X, Ha B, Wang S, Chen Z, Ge J, Long H, He W, Da W, Nian X, Yi M (2018) The earliest human occupation of the high-altitude Tibetan plateau 40 thousand to 30 thousand years ago. Science 362:1049–1051
Zhang Z, Chen Z, Marshall F, Lu H, Lemoine X, Wangyal T, Dorje T, Liu X (2019) The importance of localized hunting of diverse animals to early inhabitants of the Eastern Tibetan Plateau at the Neolithic site of Xiaoenda. Quat Int. https://doi.org/10.1016/j.quaint.2019.09.019
Zhao Z (2004) Flotation-field work method of Paleoethnobotany. Archaeology 3:80–87 (in Chinese)
Zhao Z (2014) Acquisition and utilization of plant resources. In: Institute of Archaeology, Chinese Academy of Social Sciences (ed) Erlitou (1999-2006). Cultural Relics Press, Beijing, pp 1295–1313 (in Chinese)
Zhao Z, Chen J (2011) Results of flotation of Yingpanshan site, Maoxian, Sichuan Province. Southern Cult Relics 3:60–67 (in Chinese)
Zhou X, Li X, Dodson J, Zhao K (2016) Rapid agricultural transformation in the prehistoric Hexi corridor, China. Quat Int 426:33–41
Zhou X, Yu J, Spengler RN, Shen H, Zhao K, Ge J, Bao Y, Liu J, Yang Q, Chen G, Jia PW, Li X (2020) 5,200-year-old cereal grains from the eastern Altai Mountains redate the trans-Eurasian crop exchange. Nat Plants 6:78–87
Acknowledgements
The authors thank Jingang Yang for his help with archaeobotanical identification, Qian Zhang and Jiafen Cheng for their assistance in collecting samples and carrying out flotation. We are also grateful to Bin Tang for providing the survey map, to Xingyi Guo for support in visual presentation, and to Dr. Richard Staff for conducting small graphite radiocarbon analyses at ORAU. Special thanks are given to Dr. Robert Spengler for proofreading the manuscript and providing insightful comments in the review process. The research was supported by the General Programs of the National Social Science Fund of China (Grant No. 16BKG001) and Chinese Academy of Sciences (the Strategic Priority Research Program, Grant No. XDA2004010104). We would also like to acknowledge the European Research Council (Grant No. 249642, PI: M.K. Jones) and the National Science Foundation (Grant No. 1826727, PI: X. Liu) for support.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Song, J., Gao, Y., Tang, L. et al. Farming and multi-resource subsistence in the third and second millennium BC: archaeobotanical evidence from Karuo. Archaeol Anthropol Sci 13, 47 (2021). https://doi.org/10.1007/s12520-021-01281-9
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s12520-021-01281-9