Abstract
A full-scale integrated-bioreactor consisting of a suspended zone and an immobilized zone was employed to treat the ordours emitted from a wastewater treatment plant. The inlet concentrations of H2S and NH3 were 1.6–38.6 mg∙m–3 and 0.1–6.7 mg∙m–3, respectively, while the steady-state outlet concentrations were reduced to 0–2.8 mg∙m–3 for H2S and 0–0.5 mg∙m–3 for NH3. Both H2S and NH3 were eliminated effectively by the integrated-bioreactor. The removal efficiencies of H2S and NH3 differed between the two zones. Four species of microorganisms related to the degradation of H2S and NH3 were isolated. The characteristics and distributions of the microbes in the bioreactor depended on the inlet concentration of substrates and the micro-environmental conditions in the individual zones. Product analysis indicated that most of the H2S was oxidized into sulfate in the immobilized zone but was dissolved into the liquid phase in the suspended zone. A large amount of NH3 was converted into nitrate and nitrite by nitration in the suspended zone, whereas only a small amount of NH3 was transferred to the aqueous phase mainly by absorption or chemical neutralization in the immobilized zone. Different microbial populations dominated the individual zones, and the major biodegradation products varied accordingly.
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References
Omri I, Bouallagui H, Aouidi F, Godon J J, Hamdi M. H2S gas biological removal efficiency and bacterial community diversity in biofilter treating wastewater odor. Bioresource Technology, 2011, 102(22): 10202–10209
Xi J, Kang I, Hu H, Zhang X. A biofilter model for simultaneous simulation of toluene removal and bed pressure drop under varied inlet loadings. Frontiers of Environmental Science & Engineering, 2015, 9(3): 554–562
Baspi A B, Turker M, Hocalar A, Ozturk I. Biogas desulphurization at technical scale by lithotrophic denitrification: integration of sulphide and nitrogen removal. Process Biochemistry, 2011, 46(4): 916–922
Chen Y, Fan Z, Ma L, Yin J, Luo M, Cai W. Performance of three pilot-scale immobilized-cell biotrickling filters for removal of hydrogen sulfide from a contaminated air steam. Saudi Journal of Biological Sciences, 2014, 21(5): 450–456
Burgess J E, Parsons S A, Stuetz R M. Developments in odour control and waste gas treatment biotechnology: a review. Biotechnology Advances, 2001, 19(1): 35–63
Ralebitso-Senior T K, Senior E, Di Felice R, Jarvis K. Waste gas biofiltration: advances and limitations of current approaches in microbiology. Environmental Science & Technology, 2012, 46(16): 8542–8573
Chitwood D E, Devinny J S, Reynolds F E. Evaluation of a twostage biofilter for treatment of POTW waste air. Environmental Progress & Sustainable Energy, 1999, 18(3): 212–221
van Lith C, Leson G, Michelsen R. Evaluating design options for biofilters. Journal of the Air & Waste Management Association, 1997, 47(1): 37–48
Zhang L, Ma J, Jin Y, Zhang H, Liu Y, Cai L. Abatement of sulfide generation in sewage by glutaraldehyde supplementation and the impact on the activated sludge accordingly. Frontiers of Environmental Science & Engineering, 2015, 9(2): 365–370
Li Y, Shi L, Qian Y, Tang J. Diffusion of municipal wastewater treatment technologies in China: a collaboration network perspective. Frontiers of Environmental Science & Engineering, 2017, 11 (1): 11
Li F, Lei T, Zhang Y, Wei J, Yang Y. Preparation, characterization of sludge adsorbent and investigations on its removal of hydrogen sulfide under room temperature. Frontiers of Environmental Science & Engineering, 2015, 9(2): 190–196
Liang Y, Quan X, Chen J, Chung J S, Sung J Y, Chen S, Xue D, Zhao Y. Long-term results of ammonia removal and transformation by biofiltration. Journal of Hazardous Materials, 2000, 80(1–3): 259–269
Rabbani K A, Charles W, Kayaalp A, Cord-Ruwisch R, Ho G. Pilotscale biofilter for the simultaneous removal of hydrogen sulphide and ammonia at a wastewater treatment plant. Biochemical Engineering Journal, 2015, 107: 1–10
Mannucci A, Munz G, Mori G, Lubello C. Biomass accumulation modelling in a highly loaded biotrickling filter for hydrogen sulphide removal. Chemosphere, 2012, 88(6): 712–717
Both G J, Gerards S, Laanbroek H J. Most probable numbers of chemolitho-autotrophic nitrite-oxidizing bacteria in well drained grassland soils: stimulation by high nitrite concentrations. FEMS Microbiology Ecology, 1990, 74(4): 287–293
Cho K S, Zhang L, Hirai M, Shoda M. Removal characteristics of hydrogen sulphide and methanethiol by Thiobacillus sp. isolated from peat in biological deodorization. Journal of Fermentation and Bioengineering, 1991, 71(1): 44–49
Moriarty D J, Nicholas D J. Products of sulphide oxidation in extracts of Thiobacillus concretivorus. Biochimica et Biophysica Acta, 1970, 197(2): 143–151
Chung Y C, Huang C, Tseng C P. Operation optimization of Thiobacillus thioparus CH11 biofilter for hydrogen sulfide removal. Journal of Biotechnology, 1996, 52(1): 31–38
Li L, Zhang J, Lin J, Liu J. Biological technologies for the removal of sulfur containing compounds from waste streams: bioreactors and microbial characteristics. World Journal of Microbiology & Biotechnology, 2015, 31(10): 1501–1515
Wang L, Wei B, Chen Z, Deng L, Song L, Wang S, Zheng D, Liu Y, Pu X, Zhang Y. Effect of inoculum and sulfide type on simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine slurry and microbial mechanism. Applied Microbiology and Biotechnology, 2015, 99(24): 10793–10803
Devinny J S, Deshusses M A, Webster T S. Biofiltration for Air Pollution Control. New York: Crc Pr Inc, 1999
Mudliar S, Giri B, Padoley K, Satpute D, Dixit R, Bhatt P, Pandey R, Juwarkar A, Vaidya A. Bioreactors for treatment of VOCs and odours—A review. Journal of Environmental Management, 2010, 91(5): 1039–1054
Liu D, Hansen MJ, Guldberg L B, Feilberg A. Kinetic evaluation of removal of odorous contaminants in a three-stage biological air filter. Environmental Science & Technology, 2012, 46(15): 8261–8269
Esposito R. Genium’s Handbook of Safety, Health, and Environmental Data (for Common Hazardous Substances). New York, NY: McGraw-Hill, Genium Publishing Corporation, 1999
Liu J W, Zhao Y Z, Ma W L. Removal of ammonia from waste gases by a biotrickling filter. Advanced Materials Research, 2011, 233–235: 759–764
Ministry of Environmental Protection of China. Standard Methods for the Examination of Water and Wastewater. 4th ed. Beijing, China: Chinese Environmental Science Publishers, 2002
Lin L, Liu J X, Wang J L, Pan X L. Identification and characteristic analysis of microorganisms in an integrated bioreactor for odours treatment. International Journal of Environment and Pollution, 2009, 37(1): 216–234
Kanagawa T, Mikami E. Removal of methanethiol, dimethyl sulfide, dimethyl disulfide, and hydrogen sulfide from contaminated air by Thiobacillus thioparus TK-m. Applied and Environmental Microbiology, 1989, 55(3): 555–558
Cho K S, Ryu HW, Lee N Y. Biological deodorization of hydrogen sulfide using porous lava as a carrier of Thiobacillus thiooxidans. Journal of Bioscience and Bioengineering, 2000, 90(1): 25–31
Shinabe K, Oketani S, Ochi T, Matsumura M. Characteristics of hydrogen sulfide removal by Thiobacillus thiooxidans KS1 isolated from a carrier-packed biological deodorization system. Journal of Fermentation and Bioengineering, 1995, 80(6): 592–598
Veir J K, Schroeder E D, Chang D P Y, Scow K M. Interaction between toluene and dichloromethane degrading populations in a compost biofilter. In: Proceedings of the 89th Annual Meeting and Exhibition of the Air and Waste Management Association, Nashville. Pittsburgh: USA Air & Waste Management Association, 1996, 89
Chung Y C, Huang C, Tseng C P. Biological elimination of H2S and NH3 from wastegases by biofilter packed with immobilized heterotrophic bacteria. Chemosphere, 2001, 43(8): 1043–1050
Elias A, Barona A, Arreguy A, Rios J, Aranguiz I, Penas J. Evaluation of a packing material for the biodegradation of H2S and product analysis. Process Biochemistry, 2002, 37(8): 813–820
Easter C, Quigley C, Burrowes P, Witherspoon J, Apgar D. Odor and air emissions control using biotechnology for both collection and wastewater treatment systems. Chemical Engineering Journal, 2005, 113(2–3): 93–104
Yang Y, Allen E R. Biofiltration control of hydrogen sulfide 1. Design and operational parameters. Air & Waste, 1994, 44(7): 863–868
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The authors express their sincerely Acknowledgments to ShineWrite and Editage services center of professional editing support for the English revision of the manuscript. This work was financially supported by the National Nature Science Foundation of China (Grant No. 51478456), Scientific Research Foundation of Beijing University of Civil Engineering and Architecture (No. 00331615020) and Beijing Municipal Science and Technology Commission (D151100005115002).
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Liu, J., Yang, K., Li, L. et al. A full-scale integrated-bioreactor with two zones treating odours from sludge thickening tank and dewatering house: performance and microbial characteristics. Front. Environ. Sci. Eng. 11, 6 (2017). https://doi.org/10.1007/s11783-017-0932-8
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DOI: https://doi.org/10.1007/s11783-017-0932-8