Abstract
In this paper, half-cylindrical-shaped rods are arranged in a row in order to form hemoglobin concentration sensors. The proposed structures can effectively detect hemoglobin concentrations in blood samples. Five individual structures based on graphene-plasmonic combinations are proposed and investigated. The proposed structures are made of different layers of Au, Ag, SiO2, and graphene. Different plates and cylindrical-shaped graphene layers are introduced in the structures to improve the functionality (absorption peak value and wavelength) of the absorber (sensor). Adding more Au layers strengthens the confinement of the incident electromagnetic waves and improves the absorption factor. Also, in the proposed structures, for improving the results, the effects of the chemical potential of graphene layers and “G” (graphene layer thicknesses) on the absorption peak and wavelength are considered. The final suggested structure indicates unity absorption peak and thus can be utilized in wide ranges of applications. As a refractive index bio-sensor, the structure is considered for detecting hemoglobin concentrations in blood samples which indicates a reasonable sensitivity factor of 570 nm/RIU.
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Introduction
Nowadays, optical bio-sensors have attracted excessive attentions. Optical bio-sensors indicate much impressive functionalities compared to the traditional ones. They acquire the advantages of higher sensitivity, lower cost, smaller sizes, and higher sensitivity precisions [1]. These sensors can specifically be applied in healthcare systems, biotechnology labs, and other scientific regions [2]. Optical bio-sensors can be the result of the graphene-plasmonic combinations [3]. Plasmonic-graphene nano-structures can operate based on surface plasmon resonances (SPR) [4,5,6]. Graphene-plasmonic combinations contain interesting specifications including high optical confinement [3], very low losses, and electromagnetic (EM) tunability, which make them great candidates for sensors in THz spectral range [7]. Plasmonic nano-structures are made of the combinations of metals and dielectrics, while graphene contains layers of carbon atoms being arranged in two-dimensional lattices [7, 8]. Surface plasmon resonance (SPR), which is the interaction between free electrons of a metal and the EM light wave, can conquer the limitations of light caused by the diffraction of light in nano-scaled dimensions (lower than the propagating wavelengths). As a result, structures made of graphene-plasmonic can effectively be used as absorbers [9, 10], refractive index and temperature sensors [11], or bio-sensors for detection of important elements like hemoglobin [8] and glucose [12]. Graphene-plasmonic-based nano-structures can be fabricated based on different techniques [13, 14]. The plasmonic-graphene-based sensors can be designed based on different approaches. Refractive index (RI)–based structures are good candidates for designing accurate and sensitive biosensors [15, 16]. Interesting researches were reported considering different configurations of plasmonic-graphene nanostructures. As an example, optical properties of plasmonic materials in the shape of gold nano-rods were investigated by considering the finite element method (FEM) [17]. The spatially oscillatory patterns were observed on the surface as a result of plasmon-mode wave functions [17]. In another research [18], numerical investigations of tunable SPRs and diploe cavity plasmon modes of the scattering cross-section (SCS) on different configurations of nano-rods were considered by utilizing the FEM method. Considering the different properties of these structures, the solid-gold/gold-shell nano-rods can be tuned and controlled for producing specific wavelengths [18]. In another work, a sensor based on the plasmonic-dielectric combinations with different dielectric core plasmonic metamaterial was proposed and investigated [19]. The proposed structure functions based on the combination of lattice resonance, localized SPR, and cavity plasmon resonance modes and indicated very interesting and functional results [19].
In another research, an SPR biosensor for detection of blood glucose concentrations in the range of 25–175 mg/dl with RI range of 1 to 1.0007 was proposed [20]. The proposed sensor exhibited an appropriate sensitivity amount of 275.15˚/RIU. In recent researches, considering the COVID-19 pandemic, graphene-based biosensors were suggested for virus detection as they were more economic, accurate, and fast [20]. In another study, a graphene-plasmonic biosensor was suggested and analyzed. The structure was based on the combination of silver nanoparticles and graphene film layers. Considering the graphene thickness of 9 nm in the structure, the 304.6% gain in sensitivity compared to non-graphene structure was obtained [21]. Also, plasmonic nano-structures with different shapes (of sphere, cubic, cylindrical) were proposed for hemoglobin concentration sensing [22]. The results indicated different resonance wavelengths for the three nano-particle shapes. It is declared that, by managing and engineering tunable properties of graphene-based nano-structures, they can be effectively used as biosensors for different applications [23]. In another research, graphene-gold combination structure is proposed for detecting bio-molecules and biological cells like blood, glucose, and other elements, considering their refractive indices [24].
In all of the mentioned reports, the applications of graphene-plasmonic nano-structures in sensors and especially biosensors were stated and considered. In this work, novel sensitive biosensors based on graphene-plasmonic combinations are proposed.
Geometry, Theoretical Model, and Numerical Method
The first proposed structure is a combination of graphene, Au, Ag, and SiO2 layers as depicted in Fig. 1.
The structure is consisted of the periodic pattern of half-cylindrical-shaped arrays of Au rods placed above Ag, graphene, and SiO2 layers. The geometric parameters of Fig. 1 are tabulated in Table 1.
Numerical simulations were conducted by using two-dimensional finite element-based (FE) software (COMSOL Multiphysics 5.5). As the length of the half-cylindrical nano-rods is much longer than their diameters, the 3-D model can be replaced by a 2-D model (with approximately the same results) [25, 26]. These simplifications were done for reducing the time and decreasing the computer resources needed for simulations [25, 26].
In the structure as shown in Fig. 1a, a plane wave polarized in z-axis with 1-W power is applied as the incident field. The periodic boundary condition is considered in x-direction.
Considering the finite element (FEM) method, the field distributions at resonance wavelength of 1053 nm, in Fig. 2a; non-resonance wavelength of 1040 nm, in Fig. 2b; and the absorption and reflection spectrum, in Fig. 2c, are simulated and shown.
A simple equation can show the relation between the transmission, absorption, and reflection fields [16, 27]:
Considering T(ω) = 0, then from (1) it is clear that A(ω) = 1 − R(ω).
As it is considered to achieve the perfect absorption parameter (to have the proper sensor), the reflection parameter R(ω) should equal zero which leads to unity value for absorption (A(ω)). As a result, the incident light wave would be thoroughly absorbed leading to the increment of the absorption peak value.
The surface conductivity of a graphene layer can be considered by the Kubo formula [16, 28]:
where ɷ, μc, Γ, and T indicate the operation frequency, the chemical potential, the phenomenological scattering rate, and the absolute temperature, respectively.
The intra-band and inter-band electro-photon scattering parameters can also be described as [16, 28]:
where kB, T, and e are Boltzmann constant, temperature, and electron charge, respectively.
Drude model can introduce the dielectric function of the metals as [29,30,31]:
where ε∞ = 3.7 (dielectric constant at infinite frequency), γ = 0.018 eV (collision plasma angular frequency), and ωp = 9.1 eV (bulk plasma angular frequency) [29,30,31].
As can be seen from Fig. 2 (c), the absorption peak of 0.8 at 1053 nm is obtained. To improve the absorption peak and functionality of the structure, other combinations of graphene and plasmonic layers would be presented.
Results and Discussion
In this section, 4 different structures based on the combinations of graphene-plasmonic layers in the shape of half-cylindrical arrays are presented. It is important to notice that considering more layers of graphene and Au can affect the characteristics of the structures and increase their absorption peaks [27, 32]. Therefore, their effects on the absorption spectrum will be discussed. The first structure was considered and evaluated in Fig. 1. The absorption peak value of 0.8 at wavelength of 1053 nm was achieved. The following proposed structures are proposed in order to improve the functionality of the structure by enhancing the absorption peak.
Second Structure
The second proposed structure is shown in Fig. 3. In this structure, a graphene layer is considered above the half-cylindrical arrays.
In this structure, another layer of graphene is coated above the Au half-cylindrical-shaped layers. The height of “h” equals 495 nm. The thickness of the added graphene layer is the denoted by “g” and equals to 0.34 nm.
By considering different values for the chemical potential from 0.3 to 1.5 eV, the absorption spectrum would be achieved as shown in Fig. 4.
Comparing Fig. 4 with Fig. 2c, the absorption peak value is increased from 0.8 to 0.88. Increment of the absorption peak by adding a graphene layer can be the result of lowering the losses and enhancing the confinement of light. In fact, adding more graphene and plasmonic layers can increase the confinement of light and decrease the losses, which leads to higher peak values; this phenomenon continues until N becomes more than 3, where N indicates the number of graphene layers. For N > 3, the absorption peak would be decreased. In this condition, the graphene loss surpasses the metal loss; therefore, more energy can pass to outer layers and causes the absorption peak to descend [3, 32]. As a result, maximum 3 graphene layers are considered in the following structures.
As can be seen from Fig. 4, by increasing µc, the absorption peak wavelength is shifted to the lower values. This result can be described by the circuit theory. In the circuit theory, graphene is characterized as a shunt admittance which can be varied by the geometrical parameters g, p, and chemical potential µc [16, 33]. Thus, altering µc can change the absorption peak wavelength according to [16, 33, 34]:
where c, L, and C are the speed of the light in vacuum, capacitance, and inductance of the circuit respectively. As can be concluded, increasing µc would decrease L [35], which would decrease the absorption peak wavelength. By varying µc from 0.3 to 1.5 eV, the wavelength would be shifted from 1053 to 10,495 nm. Therefore, by selecting the appropriate µc, different resonance wavelengths for different applications can be achieved.
In this structure, by adding another layer of graphene, the absorption peak is improved. This happens due to the decrease of the Au loss and excessive confinement of the light in the Au layer.
Third Structure
The third proposed structure is shown in Fig. 5.
In this structure, a layer of Au is coated above the graphene layer with the thickness of “G.” As discussed in the previous sections, adding an Au layer leads to the increased confinement of light which increases the absorption peak [3, 32]. To discuss the effects of the added Au layer, its effects on the absorption spectrum are studied. The simulation results are plotted in Fig. 6.
As can be seen, by increasing “G,” the absorption peak wavelength is shifted to the higher values. This happens due to the fact that increasing “G” would increase the capacitance; thus, according to (6), the wavelength would be shifted to the higher values [16, 33].
Fourth Structure
The fourth proposed structure is shown in Fig. 7.
In this structure, another layer of graphene is covered above the Au layer with the thickness of “g” (all the graphene layers’ thickness equal “g”). For this structure, by changing the chemical potential, the absorption spectrum can be depicted in Fig. 8.
As can be seen, increasing µc would increase the absorption peak value and decrease the absorption peak wavelength. It was stated in the previous sections that increasing µc would decrease the inductance amount (L) as the shunt admittance of the circuit theory [16, 34]. Therefore, the absorption peak value would be about 0.98 for µc = 1.5 eV at λ = 1057.5 nm.
Fifth Structure
The last proposed structure is shown in Fig. 9.
In this structure, another layer of graphene is positioned under the Au-graphene half-cylindrical-shaped arrays with the thickness of “g.” The effects of “µc” on the absorption spectrum for the last proposed structure are plotted in Fig. 10.
As previously mentioned, “µc” affects the equivalent inductance “L” of the circuit theory (increasing “µc” decreases “L”), which leads to the blue shift of the absorption peak wavelength (shifts the wavelength to lower amounts). In the last structure, the absorption peak reaches unity. As a result, this structure can be considered the bio-sensor for detecting hemoglobin concentrations.
To consider the structure as the biosensor, the absorption spectrum for different concentrations of hemoglobin with various refractive indices is shown in Fig. 11.
These refractive indices of 1.335, 1.34, 1.36, and 1.38 are related to different hemoglobin concentrations in blood samples (n = 1.335 for 3.5 g/dl, n = 1.34 for 10.6 g/dl, n = 1.36 for 16.5 g/dl, n = 1.385 for 28.7 g/dl [15, 36]).
As can be seen in Fig. 11, increasing RI would shift the absorption peak wavelength to higher amounts [3, 16, 37]. Considering the sensitivity relation [Δλ/Δn (nm/RIU)], the sensitivity value of 570 nm/RIU can be achieved for this sensor. Table 2 indicates the comparison between our proposed sensors with some previously published works.
Conclusion
Five different configurations based on graphene-plasmonic nano-structures were considered for bio-sensing applications. These structures were made of half-cylindrical-shaped rods of graphene and Au which were organized on different layers of Ag, SiO2, and graphene. In order to improve the functionality of the structures, effects of chemical potential and graphene layer’s thicknesses on the absorption peak and wavelength were also studied. By applying more cylindrical-shaped layers of Au and graphene, the absorption peaks experienced higher values. The final suggested structure, which showed unity absorption peak, was considered the refractive index sensor for detecting hemoglobin concentrations in blood samples. By considering RI values of n = 1.335 for 3.5 g/dl, n = 1.34 for 10.6 g/dl, n = 1.36 for 16.5 g/dl, n = 1.385 for 28.7 g/dl, the acceptable sensitivity factor of 570 nm/RIU was achieved. As a result, the proposed structures can be interesting and great candidates for the detection of different bio-elements and tissues and can be appropriate bio-sensors.
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Chahkoutahi, A., Emami, F. & Rafiee, E. Sensitive Hemoglobin Concentration Sensor Based on Graphene-Plasmonic Nano-structures. Plasmonics 17, 423–431 (2022). https://doi.org/10.1007/s11468-021-01531-5
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DOI: https://doi.org/10.1007/s11468-021-01531-5