Abstract
Recently, significant technical breakthroughs in both hardware equipment and software algorithms have enabled cryo-electron microscopy (cryo-EM) to become one of the most important techniques in biological structural analysis. The technical aspects of cryo-EM define its unique advantages and the direction of development. As a rapidly emerging field, cryo-EM has benefitted from highly interdisciplinary research efforts. Here we review the current status of cryo-EM in the context of structural biology and discuss the technical challenges. It may eventually merge structural and cell biology at multiple scales.
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Wang, H. Cryo-electron microscopy for structural biology: current status and future perspectives. Sci. China Life Sci. 58, 750–756 (2015). https://doi.org/10.1007/s11427-015-4851-2
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DOI: https://doi.org/10.1007/s11427-015-4851-2