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Protein Structural Analysis by Cryogenic Electron Microscopy

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Advanced Methods in Structural Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2652))

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Abstract

Cryogenic electron microscopy (cryo-EM) is constantly developing and growing as a major technique for structure determination of protein complexes. Here, we detail the first steps of any cryo-EM project: specimen preparation and data collection. Step by step, a list of material needed is provided and the sequence of actions to carry out is given. We hope that these protocols will be useful to all people getting started with cryo-EM.

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Author information

Authors and Affiliations

  1. SciLifeLab Cryo-EM Facility, Department of Chemistry, Umeå University, Umeå, Sweden

    Michael Hall, Erin Schexnaydre & Camilla Holmlund

  2. SciLifeLab Cryo-EM Facility, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden

    Marta Carroni

Authors
  1. Michael Hall
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  2. Erin Schexnaydre
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  3. Camilla Holmlund
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  4. Marta Carroni
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Corresponding authors

Correspondence to Michael Hall or Marta Carroni .

Editor information

Editors and Affiliations

  1. Universidade da Beira Interior, CICS-UBI, Covilhã, Portugal

    Ângela Sousa

  2. University of Beira Interior, Covilhã, Portugal

    Luis Passarinha

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Hall, M., Schexnaydre, E., Holmlund, C., Carroni, M. (2023). Protein Structural Analysis by Cryogenic Electron Microscopy. In: Sousa, Â., Passarinha, L. (eds) Advanced Methods in Structural Biology. Methods in Molecular Biology, vol 2652. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3147-8_24

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  • DOI: https://doi.org/10.1007/978-1-0716-3147-8_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3146-1

  • Online ISBN: 978-1-0716-3147-8

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