Abstract
Complications arising from tendon injury include tendon sheath infection and peritendinous adhesion, in which tendon adhesion often leads to serious motor dysfunction. In this work, the electrospun membranes of poly(L-lactide) (PLA) and poly(ε-caprolactone) (PCL) with different degradation kinetics were used to investigate their efficacy for anti-adhesion toward Achilles tendon repair. Compared with the PCL membrane, the PLA sample showed a faster rate of degradation in 42 d, and all the degradation media (i.e., phosphate-buffered saline) maintained at a constant pH of around 7.4. Meanwhile, the superior biocompatibility of both the PLA and PCL membranes were proved by the in vitro cellular adhesion tests and in vivo histopathological assays. Simultaneously, the PLA membrane was more effective than the PCL sample in decreasing adhesion and promoting functional recovery. Furthermore, the experiment result was further confirmed by hematoxylin-eosin and Masson’s trichrome staining, and type I collagen immunohistochemical analysis. All results revealed that the model treated with the electrospun PLA membrane was obviously better with regard to both anti-adhesion and tendon repair than that in the PCL membrane group. Considering the results of degradation and adhesion prevention efficacy, the electrospun polyester membranes, especially the PLA one, would be applied with fascinating potential in clinical prevention of postoperative tendon adhesion.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
McGonagle L, Jones MD. The bio-tribological properties of antiadhesive agents commonly used during tendon repair. J Orthop Res, 2012, 30: 775–780
Wong JK, Lui YH. The cellular biology of flexor tendon adhesion formation: an old problem in a new paradigm. Am J Pathol, 2009, 175: 1938–1951
Zhao C, Amadio PC. The effect of suture technique on adhesion formation after flexor tendon repair for partial lacerations in a canine model. J Trauma, 2001, 51: 917–921
Momose T, Amadio PC. Surface modification of extrasynovial tendon by chemically modified hyaluronic acid coating. J Biomed Mater Res, 2002, 59: 219–224
Liu S, Qin M. Tendon healing and anti-adhesion properties of electrospun fibrous membranes containing bFGF loaded nanoparticles. Biomaterials, 2013, 34: 4690–4701
Hu C, Liu S. Long-term drug release from electrospun fibers for in vivo inflammation prevention in the prevention of peritendinous adhesions. Acta Biomater, 2013, 9: 7381–7388
Mourya VK, Inamdar NN. Carboxymethyl chitosan and its applications. Adv Mater Lett, 2010: 1, 11–33
Li L, Wang N. Biodegradable and injectable in situ cross-linking chitosan-hyaluronic acid based hydrogels for postoperative adhesion prevention. Biomaterials, 2014, 35: 3903–3917
Siddiqi NA, Hamada Y. Effects of hydroxyapatite and alumina sheaths on postoperative peritendinous adhesions in chickens. J Appl Biomater, 1995, 6: 43–53
Khanna A, Friel M. Prevention of adhesions in surgery of the flexor tendons of the hand: what is the evidence? Brit Med Bull, 2009, 90:85–109
Way TD, Hsieh SR. Preparation and characterization of branched polymers as postoperative anti-adhesion barriers. Appl Surf Sci, 2010, 256: 3330–3336
Diamond MP, Luciano A. Reduction of postoperative adhesions by N,O-carboxymethylchitosan: a pilot study. Fertil Steril, 2003, 80: 631–636
Agarwal S, Wendorff JH. Progress in the field of electrospinning for tissue engineering applications. Adv Mater, 2009, 21: 3343–3351
Rafiei S, Maghsoodloo S. Mathematical modeling in electrospinning process of nanofibers: a detailed review. Cellul Chem Technol, 2013, 47: 323–338
Andersson L, Jones AC. Three-dimensional structure analysis by X-ray micro-computed tomography of macroporous alumina templated with expandable microspheres. J Eur Ceram Soc, 2010, 30: 2547–2554
Xing ZC, Han SJ. Fabrication of biodegradable polyester nanocomposites by electrospinning for tissue engineering. J Nanomater, 2011, 2011: 1–18
Zheng R, Duan H. The influence of Gelatin/PCL ratio and 3-D construct shape of electrospun membranes on cartilage regeneration. Biomaterials, 2014, 35: 152–164
Bölgen N, Vargel I. In vivo performance of antibiotic embedded electrospun PCL membranes for prevention of abdominal adhesions. J Biomed Mater Res B, 2007, 81: 530–543
Dinarvand P, Hashemi SM. Function of poly (lactic-co-glycolic acid) nanofiber in reduction of adhesion bands. J Surg Res, 2012, 172: e1–e9
Lee SJ, Oh SH. The use of thermal treatments to enhance the mechanical properties of electrospun poly(e-caprolactone) scaffolds. Biomaterials, 2008, 29: 1422–1430
Wang L, Chen J. Synthesis and evaluation of biodegradable segmented multiblock poly (ether ester) copolymers for biomaterial applications. Polym Int, 2004, 53: 2145–2154
Yamaoka T, Njatawidjaja E. Elastic/adhesive double-layered PLAPEG multiblock copolymer membranes for postoperative adhesion prevention. Polym Degrad Stabil, 2013, 98: 2168–2176
Jiang S, Wang W. Prevention of intra-abdominal adhesion by bi-layer electrospun membrane. Int J Mol Sci, 2013, 14: 11861–11870
Yang DJ, Chen F. Tissue anti-adhesion potential of biodegradable PELA electrospun membranes. Acta Biomater, 2009, 5: 2467–2474
Jiang S, Wang W, Yan H, Fan C. Prevention of intra-abdominal adhesion by bi-layer electrospun membrane. Int J Mol Sci, 2013, 14: 11861–11870
Zhang J, Liu H. Molecular weight-modulated electrospun poly(ε- caprolactone) membranes for postoperative adhesion prevention. RSC Adv, 2014, 4: 41696–41704
Dunne M, Corrigan O. Influence of particle size and dissolution conditions on the degradation properties of polylactide-co-glycolide particles. Biomaterials, 2000, 21: 1659–1668
Li S, McCarthy S. Further investigations on the hydrolytic degradation of poly (DL-lactide). Biomaterials, 1999, 20: 35–44
Jung JH, Ree M. Acid- and base-catalyzed hydrolyses of aliphatic polycarbonates and polyesters. Catal Today, 2006, 115: 283–287
Saha SK, Tsuji H. Hydrolytic degradation of amorphous films of L-lactide copolymers with glycolide and D-lactide. Macromol Mater Eng, 2006, 291: 357–368
Grayson AC, Cima MJ. Size and temperature effects on poly(lacticco-glycolic acid) degradation and microreservoir device performance. Biomaterials, 2005, 26: 2137–2145
Tsuji H, Tezuka Y. Alkaline and enzymatic degradation of L-lactide copolymers, Amorphous-made films of L-lactide copolymers with D-lactide, glycolide, and epsilon-caprolactone. Macromol Biosci, 2005, 5: 135–148
Sun L, Zhang S. Fenton reaction-initiated formation of biocompatible injectable hydrogels for cell encapsulation. J Mater Chem B, 2013, 1: 3932–3939
Koob TJ, Summers AP. Tendon—bridging the gap. Comp Biochem Phys A, 2002, 133: 905–909
Fang M, Goldstein EL. Type I collagen D-spacing in fibril bundles of dermis, tendon, and bone: bridging between nano-and micro-level tissue hierarchy. ACS Nano, 2012, 6: 9503–9514
Langberg H, Skovgaard D. Type I collagen synthesis and degradation in peritendinous tissue after exercise determined by microdialysis in humans. J Physiol, 1999, 521: 299–306
Liu Y, Jiang H. Control of dimensional stability and degradation rate in electrospun composite scaffolds composed of poly(D,L-lactide-coglycolide) and poly(ε-caprolactone). Chinese J Polym Sci, 2008, 26: 63–71
Diamond MP, Burns EL. Seprafilm® adhesion barrier: (1) a review of preclinical, animal, and human investigational studies. Gynecol Surg, 2012, 9: 237–245
Diamond MP, Burns EL. Seprafilm® adhesion barrier: (2) a review of the clinical literature on intraabdominal use. Gynecol Surg, 2012, 9: 247–257
Zhang ZL, Xu S. Preventive effects of chitosan on peritoneal adhesion in rats. World J Gastroenterol, 2006, 12: 4572–4577
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Song, Z., Shi, B., Ding, J. et al. A comparative study of preventing postoperative tendon adhesion using electrospun polyester membranes with different degradation kinetics. Sci. China Chem. 58, 1159–1168 (2015). https://doi.org/10.1007/s11426-015-5425-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11426-015-5425-7