Abstract
Purpose
Vegetation restoration is an effective measure for improving the function of soil ecosystems and promoting the biogeochemical cycling of carbon (C), total nitrogen (TN) and total phosphorus (TP). Here, we aimed to quantify the fine-scale (pedon scale) spatial distribution of soil C, N, P and soil physical–chemical properties in the Mu Us Desert ecosystems.
Methods
We systematically evaluated the effects of A. ordosica on fine-scale (pedon scale) spatial distribution of C, TN, TP, soil-available nutrients, and liable organic carbon (LOC) and their stoichiometric characteristics in the semiarid Mu Us Desert in the 0–100-cm soil profiles at various distances from the plant.
Results and discussion
The results demonstrated that soil organic carbon (SOC), TN and LOC were decreased with increasing distance from the plant and soil depth. SOC stocks at 20 cm were 16.98% higher than those at 120 cm from the plant. SOC stocks at 20, 60 and 120 cm from the plant were increased by 71.62%, 58.14% and 46.72% compared with shifting sandy land (Sland), respectively. Microbial biomass carbon (MBC) and readily oxidised organic carbon (ROOC) were significantly affected by different soil layers and distances and their interaction (p < 0.05), whereas dissolved organic carbon (DOC) was affected by the soil layers. TN and soil-available nutrients in the surface layer and at closer distances to the plant were higher than those in the sublayer and Sland. The ratio of C:N:P was generally decreased with different distances from the plant and different soil layers. The ratios of soil C:N, C:P and N:P were significantly different at different soil layers, whereas the ratios of soil C:P and N:P were significantly different at different distances from the plant (p < 0.05). Soil C:P ratio was positively correlated with soil C:N and N:P ratios (p < 0.001). N and P contents in leaves were higher than those in roots, branches and litter, but C contents in leaves were lower than those in other plant tissues and litter (p < 0.01). N:P ratio in leaves (13.94) showed that there was a shortage of N and P in the Mu Us Desert ecosystems.
Conclusions
We concluded that A. ordosica could enhance the accumulation of SOC, LOC and N on a fine scale and improve mineral-nutrient availability in semiarid deserts and, as a result, the function of soil ecosystems could be improved. Moreover, the limitation of N and P can be alleviated by adding additional N and P.
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1 Introduction
Desertification is one of the most serious land degradation problems in the world, of which 30% is distributed in arid and semiarid areas (Ibanez et al. 2007). On the one hand, the expansion of desertification not only negatively affects the biogeochemical cycles of soil organic carbon (SOC), total nitrogen (TN) and total phosphorus (TP) and their stoichiometric characteristics but also reduces the primary productivity and ecological functions of regional vegetation (Zhou et al. 2008; Gu et al. 2018; Hu et al. 2018). On the other hand, vegetation restoration can significantly improve ecosystem functions in arid and semiarid desert areas (Lange et al. 2015; Hong et al. 2020). Therefore, implementing proper vegetation restoration strategies in desert areas can effectively improve ecosystem functions (Evans et al. 2014; Koyama et al. 2019), such as improving soil carbon sequestration (Gao et al. 2017, 2018; Hong et al. 2020) and nutrient availability (Li et al. 2013; Hu et al. 2018) and reversing desertification (Li et al. 2016; Zhang et al. 2016; Hong et al. 2018). Therefore, it is of great importance to understand the impact of vegetation restoration on the fine-scale (pedon scale) spatial distribution of soil C, N and P and physical–chemical properties in desert ecosystems.
The biogeochemical cycles of C, N and P and their stoichiometric characteristics are affected by various biotic and abiotic factors (Chaopricha and Marín-Spiotta 2014; Lai et al. 2016; Liu et al. 2020), such as land use change (Deng et al. 2014), vegetation type (Liang et al. 2017; Lorenz and Thiele-Bruhn 2019) and climatic zones (Wang et al. 2020). The contents of SOC, TN and labile organic carbon (LOC) fractions (including dissolved organic carbon (DOC), microbial biomass carbon (MBC) and readily oxidised organic carbon (ROOC)) were significantly increased after vegetation restoration (Benbi et al. 2015; Lai et al. 2016; Li et al. 2016; Gao et al. 2018; Zhang et al. 2020a), and the available N, P and K contents were increased for 38 years after vegetation restoration in Horqin Sandy Land (Li et al. 2013, 2018). Therefore, changes in SOC, TN and TP after vegetation restoration result in variation in the stoichiometric characteristics of C, N and P in different plant tissues and soils (Liang et al. 2017; Hu et al. 2018). The contents of C, N and P of plants and their stoichiometry have been considered to be a crucial indicator of N and P status, which was through the absorption of CO2 from the air by the leaves and the absorption of N and P from the soil by the roots (Delgado‐Baquerizo et al. 2016; Hu et al. 2018). Plant growth requires the absorption of large amounts of water and nutrients through the roots, which improves the content and status of available nutrients (Hu et al. 2018; An et al. 2019). However, vegetation restoration improves the soil-nutrient contents and positively affects soil microbial organisms and soil physical–chemical properties in arid and semiarid regions (Deng et al. 2017; Li et al. 2018). Hu et al. (2018) and Tian et al. (2010) indicated that N and P contents were the main limiting factors for plant growth in desert ecosystems. However, the distribution of C, N and P and their stoichiometric characteristics after vegetation restoration on a fine scale (pedon scale) are poorly understood in semiarid desert ecosystems.
To impede the deterioration of land desertification in northwest and north China, from the 1980s to the 2010s, the Chinese government implemented a series of environmental protection projects (e.g. 3-North Shelter Forest Project), which have produced actively ecological and environmental benefits (Deng et al. 2014; Bryan et al. 2018; Chu et al. 2019). These projects have significantly increased the vegetation coverage and primary productivity of ecosystems in northwest and north China and improved soil physical–chemical properties (Li et al. 2018), enriched the soil carbon pool (Deng et al. 2014; Deng and Shangguan 2017; Chu et al. 2019) and changed the biogeochemical cycles of C, N and P and their stoichiometric characteristics (Hu et al. 2018; Zhang et al. 2020b). The Mu Us Desert is a typical restoration area in northwest China where herbaceous plants, such as A. ordosica, were randomly established with aircraft seeding. However, most of the previously published literature (Lai et al. 2016; Gao et al. 2018) was focused on the different restoration years and land use–type effects on SOC sequestration and soil physical–chemical properties in this region. Limited studies have evaluated the effects of A. ordosica on the fine-scale (pedon scale) spatial distribution of C, N and P and their stoichiometric characteristics.
Keeping in mind the above issues, the present study was conducted to assess the effects of vegetation restoration on C, N and P and their stoichiometric characteristics on the fine scale (pedon scale) in the Mu Us Desert. The main objectives of our study were (1) to understand the effects of A. ordosica on the function of desert soil ecosystems on the fine scale and (2) to quantify the fine-scale spatial distribution of C, N and P and physical–chemical properties and their stoichiometric characteristics in soil planted with A. ordosica in the Mu Us Desert ecosystems.
2 Materials and methods
2.1 Study area
The study was conducted at Yuyang District, Yulin, Shaanxi province (38.32°–38.37° N, 109.62°–109.82° E), on the southeast edge of the Mu Us Desert. The climate belongs to the temperate semiarid continental monsoon climate zone with an average altitude of 1,100 m a.s.l.. The average annual precipitation and temperature are 430 mm and 9.1℃, respectively, and precipitation mainly occurs from July to September. The dominant soil type is Cambic Arenosols (FAO 2006). A. ordosica is one of the most typical vegetation types in this region and has a strong resistance to stress conditions, such as salinity, wind and sand and features rapid growth and well-developed root systems.
2.2 Experimental design and sample collection
In September 2019, three sites of the A. ordosica community were selected, and the distance between any two sites was greater than 200 m (Fig. 1A). Therefore, the vegetation coverage of the study area was less than 50%. Furthermore, for each site, a plot of 30 × 30 m2 was selected, which included three plants of A. ordosica, each of which was measured for height, crown diameter, branch diameter and basal stem diameter; the results are shown in Table 1. The soil samples were collected with a soil auger having a diameter of 6 cm at depths of 0–10, 10–20, 20–40, 40–60 and 60–100 cm from the eastern, western, southern and northern directions around an A. ordosica plant in fine scale (pedon scale) at 20-, 60- and 120-cm distances from the plant; litter thicknesses were also determined. In addition, the roots and fresh soil samples were collected from the eastern direction for each selected plant (Fig. 1B). Moreover, three shifting sandy land (Sland) samples were selected as controls from Sland near the sampling sites. The branches, leaves and litter from each selected plant were also collected. Branch and leaf samples consisted of five branches and all mature leaves from each selected branch. A mixed sample of litter was collected from four directions (east, west, north and south) for each selected plant, and the litter collection area was 10 cm × 10 cm. To determine the soil bulk density (BD), a metal core of 100 cm3 was used to collect a soil sample from the above soil layers at a 60-cm distance from the plant in the eastern direction.
2.3 Field and laboratory methods
Plant root samples were taken to the laboratory, where they were washed and separated with tweezers and a 0.2-mm sieve. Root length density (RLD) was scanned with an EPSON model V700 and analysed by WinRHIZO software (Regent Instruments Inc.). The root, leaf, branch and litter samples were oven-dried at 65 ℃ ± 3 ℃, crushed and passed through a 0.15-mm sieve. Fresh soil samples from the eastern direction were divided into two portions, one stored at 4 ℃ for analysing DOC, MBC, microbial biomass nitrogen (MBN), nitrate nitrogen (NO3−–N) and ammonium nitrogen (NH4+–N) within 10 days. The remaining soil samples were air-dried at room temperature and then passed through 1-mm and 0.25-mm sieves. SOC, soil inorganic carbon (SIC), TN and TP were determined using 0.25-mm soil samples, and soil physical–chemical properties were determined using 1-mm soil samples.
The elemental analyser determined the C and N contents of the root, leaf, branch and litter samples (Elementar Vario TOC/TNb Analyzer, Germany). SOC was determined by the potassium dichromate–sulfuric acid external heating method (Nelson and Sommers 1996). SIC was determined by the gas methods (Wang et al. 2012). Soil TN was determined by the Kjeldahl method (Page et al. 1982). TP contents of soil and plant samples were digested with H2SO4–HClO4 and determined by a spectrophotometer (Shimadzu TOC-L, Japan). Soil pH and EC (soil:water ratio 1:2.5) were determined by pH/conductivity meters (EXTECH Instruments, ExStik II, USA). The soil particle size distribution was measured by a Malvern laser particle size analyser (Mastersize2000, UK) and was divided into three groups: clay + silt (Csilt, < 50 μm), fine sand (Fsand, 50–250 μm) and coarse sand (Csand, > 250 μm). Soil NO3−–N and NH4+–N concentrations were extracted with 2 mol·L−1 KCl solution and measured with a AA3 analyser (Germany). DOC and dissolved inorganic carbon (DIC) were extracted according to van Agtmaal et al.’s (2017) methods for extraction. MBC and MBN were fumigated with chloroform and incubated for 24 h (Joergensen 1996); then, 0.5 mol·L−1 K2SO4 40 mL with 10 g fresh soil at 200 r·min−1 was oscillated for 30 min and then filtered. A TOC analyser determined the filtrate of DOC, DIC and MBC (Shimadzu TOC-L, Japan), and MBN was determined using the AA3 analyser. ROOC was determined by 333 mmol·L−1 KMnO4 oxide methods (Blair et al. 1995). Soil BD was measured by oven dry 105 ℃ with a 100-cm3 metal core soil samples.
The following formula was used to calculate the SOC and SIC stocks:
where SOCs (SICs, kg·m−2) is the SOC (SIC) stocks; δ (%) is the content of particles > 2 mm; Ci (g kg−1) is the organic/inorganic carbon content; h (cm) is the thickness of each layer of soil; γi (g cm−3) is the soil bulk density and 100 is a conversion coefficient.
2.4 Statistical analysis
The Shapiro–Wilk test was used to check the normality and homoscedasticity of all data. SOC, TN, TP and soil physical–chemical parameters were compared by two-way ANOVA with LSD post hoc tests to investigate the effects of distances from the plant and soil depths. The C, N and P of plant tissues and litter and their stoichiometry were compared by one-way ANOVA with LSD post hoc tests. All statistical analyses were performed at p < 0.05. Results for all indicators were expressed as mean ± standard deviation (SD). Pearson’s correlation coefficient was used to evaluate the relationship among all the soil parameters. A principal component analysis was used to extract the four common factors for all soil parameters. All data were analysed by SPSS19.0 (SPSS Inc., Chicago, IL, USA).
3 Results
3.1 C, N and P and stoichiometric characteristics in plant tissues and litter
N contents (2.12%) in leaves were higher than those in roots (0.81%), branches (0.84%) and litter (1.45%), and lower N contents were recorded in litter (Table 2). P contents (3.59 g kg−1) in leaves were higher than those in roots (1.36 g kg−1), branches (1.12 g kg−1) and litter (1.22 g kg−1), and lower P contents were recorded in the branches (Table 2). C contents (41.90%) in leaves were lower than those in other plant tissues and litter. C:N ratios in branches (68.02) and roots (70.50) were higher than those in litter (33.84) and leaves (25.07), and a higher C:P ratio was recorded in the branches than in the litter, roots and leaves. While C:P ratios of litter and roots were higher than in leaves (Table 2). N:P ratios of litter, branches, roots and leaves were 27.93, 17.72, 13.92 and 13.94, respectively.
3.2 Soil physical–chemical properties under A. ordosica
The crown diameter of A. ordosica was 1.75 m, and the thicknesses of litter at 20, 60 and 120 cm from A. ordosica were 8.91, 2.45 and 0.00 mm (Table 1), respectively. The roots of A. ordosica were mainly distributed in the 0–20-cm soil layer within a range of 60 cm from the plant. RLD was significantly different among different soil layers (Table 3; p < 0.05), but there were no significant differences among different distances from the plant (p = 0.068). The RLD ranged from 0.03 to 1.91 cm cm−3 in the 60–100-cm soil layer at a 120-cm distance to the 0–10-cm soil layer at a 20-cm distance, respectively (Table 3).
As shown in Table 3, soil AK, AP, NO3−–N, NH4+–N and EC were highest at the soil surface layer and progressively decreased the greater the distance from the plant and the deeper the soil depth. In contrast, pH increased with soil depth (Table 3). Soil AP, pH and EC were affected by soil depth (Table S1). The distances and soil layers and their interaction significantly affected AK (Tables 3 and S1; p < 0.05). NO3−–N showed a significant difference at different soil layers (p < 0.05), whereas there were no significant differences with distances (Tables 3 and S1; p > 0.05). NH4+–N was not significantly different at different soil layers and distances (p > 0.05). However, there was a higher NH4+–N content in the 20–60-cm soil layer at a 60-cm distance from the plant (Table 3). Soil MBN was higher in the 0–20-cm soil layer and showed an increasing trend with the distances from the plant and a decreasing trend with soil depths (Table 3).
3.3 Soil C, N and P and LOC fractions and their stoichiometric characteristics
SOC, DOC, MBC and ROOC concentrations ranged from 0.33 to 1.40 g kg−1, from 8.91 to 27.04 mg kg−1, from 5.55 to 39.33 mg kg−1 and from 49.70 to 294.80 mg kg−1, respectively (Table 4). The contents of SOC, DOC, MBC and ROOC accumulated in the surface layer (0–20 cm), which were much higher than those in soil layers greater than 20 cm (Table 4). The distance from the plant and the soil layers and their interaction significantly affected SOC, MBC and ROOC (Table S1; p < 0.05). The contents of SOC, DOC, MBC and ROOC in the 0–10-cm soil layer at a 20-cm distance from the plant were significantly higher than those in Sland (Table 4). The DOC, MBC, and ROOC accounted for 2.79%, 1.88% and 19.41% of SOC, respectively. The SIC and DIC concentrations ranged from 0.15 to 0.20 g kg−1 and from 3.88 to 7.09 mg kg−1, respectively (Table 4). However, there were no significant differences in SIC and DIC at different distances and soil layers (p > 0.05).
SOC stocks at 20 cm (0.86 kg m2) from the plant were significantly higher than those at 60 cm (0.79 kg m2) and 120 cm (0.73 kg m2) and in Sland (0.50 kg m2; Fig. 2a; p < 0.05). However, SIC stocks had no significant differences among distances from the plant and in Sland (Table 4). SOC stocks at 20 cm were 16.98% (0.07 kg m2) higher than those at 120 cm from the plant. SOC stocks at 20, 60 and 120 cm were 71.62% (0.36 kg m2), 58.14% (0.29 kg m2) and 46.72% (0.24 kg m2) higher than those in Sland, respectively. SOC stocks in the southeastern direction were higher than those in the northwestern direction of the plant (p > 0.05), and SIC stocks in the eastern and western directions were slightly higher than those in the northern and southern directions (Fig. S1; p > 0.05).
Soil TN was highest at the soil surface layer and decreased with greater distance from the plant and greater soil depths (Table 4). TP content was not affected by distance from the plant and soil depths. The TN and TP concentrations ranged from 0.04 to 0.11 g kg−1 and from 0.16 to 0.20 g kg−1, respectively (Table 4). The stoichiometric characteristics of soil C:N, C:P and N:P were 6.77–17.03, 2.93–22.72 and 0.32–1.76, respectively. The ratio of C:N:P generally decreased with greater distance from the plant and deeper soil layers (Fig. 3a-c). The ratios of soil C:N, C:P and N:P varied significantly at different soil layers, whereas the ratios of soil C:P and N:P varied significantly at different distances from the plant (Table S1; p < 0.05). The ratios of soil C:N, C:P and N:P in 0–10-, 0–40- and 0–40-cm soil layers at different distances from the plant were higher than those in Sland, respectively. The ratios of soil C:P and soil N:P in the 0–40-cm layer were lower than those in soil layers greater than 40 cm (p < 0.05). The soil C:P ratio was positively correlated with the soil C:N and N:P ratios (Fig. 4a, c; p < 0.001).
3.4 The relationship among SOC, TN and TP and soil physical–chemical factors
SOC was positively correlated with TN, RLD, DOC, MBC, ROOC, MBN, EC, AK, AP, NO3−–N, NH4+–N, EC and Fsand, whereas it was negatively correlated with soil depth, pH and Csand (Table 5). TN was positively correlated with RLD, DOC, MBC, ROOC, MBN, AK, AP, NO3−–N, NH4+–N, EC and Fsand and negatively correlated with soil depth, pH and Csand (Table 5). TP was positively correlated with SIC, DIC, pH and Csilt. DOC, MBC and ROOC were all positively correlated with AK, AP, NO3−–N and EC, respectively (Table 5). Soil pH and Csand were negatively correlated with the physical–chemical factors (Table 5).
The results of principal component analysis showed that four principal components could be extracted from all parameters, accounting for 76.04% of the total load (Table 6). The first, second, third and fourth principal components contained 44.76%, 14.81%, 9.46% and 7.01% explanatory variance, respectively (Table 6). The first principal component was composed of SOC, TN, LOC, RD, available P, available K and the stoichiometric characteristics of C, N and P in soil. The second principal component was TP, DIC, SIC, Csilt and Csand; the third principal component was composed of Fsand; and the fourth principal component was composed of NH4+ and MBN (Table 6).
4 Discussion
4.1 Effects of A. ordosica on soil physical–chemical properties
Vegetation restoration is one primary critical strategy to improve the function of soil ecosystems (Hong et al. 2018, 2020) that can affect the cycle and redistribution of nutrient elements in soils (Deng et al. 2014; Zhang et al. 2020a). A. ordosica has great eco-environmental benefits that can improve the C, N and P cycle and nutrient availability in the Mu Us Desert (Lai et al. 2016; Gao et al. 2018; Li et al. 2018). That RLD was positively correlated with available nutrients suggests that A. ordosica leads to the higher availability of nutrients. The roots of A. ordosica are mainly distributed in the 0–20-cm soil layer within a range of 60 cm from the plant, which is more conducive to the rapid adaptation and utilisation of soil water during the precipitation period (Lai et al. 2016). The “fertility island” effect of A. ordosica positively changed the soil’s physical–chemical properties and spatial distribution (Chaopricha and Marín-Spiotta 2014; Hu et al. 2018). Our results showed that TN, AK, AP, NO3−–N, EC and pH have a substantial spatial heterogeneity, which supports the fertility island theory (Tables 3 and 4) and was found to be similar to the results of Li et al. (2007) and Hu et al. (2018). This finding could be due to the litter and roots of A. ordosica mainly being distributed on the surface (0–20 cm) layer, which increases soil organic matter and root exudate input as well as increases mineral nutrients from their decomposition (Lorenz and Thiele-Bruhn 2019; Zhang et al. 2020b). Therefore, organic acids and amino acids originated from organic matter decomposition, and root exudates decreased pH and increased EC and AP of the topsoil (Hong et al. 2018). However, the distribution of TP was different from that of other nutrients in the soil profiles, a finding that is explained by TP contents being dependent on soil parent materials (Wang et al. 2020). The NH4+–N had a lower concentration in the surface soil layer at less than 60 cm from the plant, a finding that may result from its mainly being absorbed by A. ordosica. Furthermore, NH4+–N concentrations were lower than those of NO3−–N in the whole soil profiles, a finding that may be because the decomposition of litter releases a large amount of NH4+–N, which were adsorbed in the soil profile. Meanwhile, NO3−–N more easily leaches downwards (Yao et al. 2019).
4.2 Effect of A. ordosica on soil carbon
Vegetation restoration is helpful for increasing SOC, SOC stocks and soil LOC contents (Li et al. 2007; Zhang et al. 2020a), which was consistent with our study. Accurate evaluation of carbon sequestration at fine scale (pedon scale) is crucial for understanding soil carbon stocks. Hong et al. (2020) pointed out that it is easier to increase SOC accumulation in poor C soil with vegetation restoration; our results also support this finding. SOC stocks at 20-, 60- and 120-cm distances from A. ordosica were much higher than those in Sland, a finding that is consistent with the study carried out in the Tengger Desert by Li et al. (2016). This is because vegetation restoration increases the input of organic matter from litter and root exudates at closer distances to the plants. However, SIC stocks at different distances were not significantly different from those in Sland (Fig. 3b; p > 0.05). The distribution of SIC stocks is dependent on the primary and secondary carbonate contents in the soil (Gao et al. 2017). SOC and LOC showed distinct “surface accumulation”, which is mainly affected by aboveground litter accumulation (litter thickness) and root distribution in the soil profile (Cotrufo et al. 2015; Lange et al. 2015). Moreover, subsurface and deeper soil layers are mainly affected by root litter and exudates, and soil particle composition (Ahmed et al. 2016; Lai et al. 2016) and surface DOC leaching (Cotrufo et al. 2015). Furthermore, SOC and LOC in the fine scale (pedon scale) are closely related to litter input and root distribution.
Ahmed et al. (2016) and Benbi et al. (2015) discovered that DOC, MBC and ROOC account for 3.1–5.2%, 1.6–3.1% and 5–30% of SOC, respectively, a finding that is consistent with our study. However, DOC was slightly lower than what was found in previous results, which may be because of low fresh organic matter input in the Mu Us Desert compared with other ecosystems. Though DOC and MBC account for relatively low proportions of SOC, both of them are the most active soil C pool (Zhang et al. 2020a). Therefore, the proportions of LOC could reflect the input of fresh carbon (Sokol et al. 2019) and soil microbial activities (Bongiorno et al. 2019). DOC and MBC are soil microorganisms’ main C and energy sources (Bongiorno et al. 2019), which play a critical role in the soil biogeochemical cycle (Lange et al. 2015). RLD was positively correlated with DOC, MBC and ROOC, confirming that root distribution promotes DOC, MBC and ROOC. Moreover, DOC, MBC and ROOC are derived from old soil organic matter decomposition (Cotrufo et al. 2015). Previous studies showed that one-third of DOC is derived from old carbon and two-thirds from fresh organic matter input (Froberg et al. 2007). The proportion of ROOC reflects the stability of SOC. The higher (or lower) the proportion of ROOC, the higher (or lower) the liable SOC (Zhang et al. 2020a). Compared with greater distances and Sland, A. ordosica increases SOC and LOC closer to the plant.
4.3 Stoichiometric characteristics of C, N and P in soils and A. ordosica
The stoichiometric characteristics of C, N and P in soils and plants are helpful to understand the nutrient status of vegetation and soils in desert ecosystems (Cotrufo et al. 2015; Hu et al. 2018; Zhang et al. 2018). The stoichiometric characteristics of SOC, N and P are reduced with increasing soil depths and distances from the plant (Fig. 4a-c); this is because of a higher litter input in the 0–40-cm soil layer and closer distance to the plant. To support the growth of A. ordosica, more available P is needed to be absorbed from deeper soil layers (Hu et al. 2018). In our study, A. ordosica mainly absorbed P from the 0–40-cm soil layer (Table 3). Güsewell (2004) discovered that P limitation occurred when the leaf N:P ratio is higher than 20, N is limited when the leaf N:P ratio is lower than 10, and N and P colimitations occurred when leaf N:P ratio is in the range of 10–20. A previous study indicated that China’s leaf N:P ratios are very limited in different climatic regions and most are in a colimitation status of N and P (Tian et al. 2010). Our results showed a significant shortage of N and P (leaf N:P = 13.94) in the semiarid Mu Us Desert ecosystems. The soil N sources mainly originate from the biological fixation and atmospheric N deposition, and P mainly derives from mineral weathering. The TP was positively correlated with inorganic carbon parameters, and AP was positively correlated with organic carbon parameters (Tables 3 and S1), which points out that TP content is dependent on soil parent materials and AP content is dependent on the release of soil parent materials and litter input. Therefore, AP release is controlled by SOC or organic acids. The root distribution of A. ordosica means more organic acids are in the topsoil, which promotes soil mineral weathering and P release from the active root zone and reduces the TP content in the subsurface soil (Gao et al. 2019). Previous studies have pointed out that N and P contents in plant tissues are closely linked to soil N and P contents and their stoichiometry (Tian et al. 2010; Gao et al. 2018). The C, N and P contents of litter are related to C, N and P stoichiometry of soil and plants (Dong et al. 2019). In summary, the distribution of SOC, TN, TP and soil nutrients in fine scale (pedon scale) is essential in understanding soil carbon accumulation and the function of the desert soil ecosystems. Moreover, the growth of A. ordosica is limited by the shortage of N and P, which is a significant factor for the vegetation restoration of the desert with A. ordosica.
5 Conclusions
In the semiarid Mu Us Desert, A. ordosica both alleviated and hindered desertification and improved the function of desert soil ecosystem by increasing the SOC, TN and LOC accumulations and soil-available mineral nutrients in fine scale (pedon scale). The roots of A. ordosica were mainly distributed in the 0–40-cm soil layer within a range of 60 cm from the plant, contributing to the biogeochemical cycle of C, N and P and nutrient availability. However, there were N and P limitations in the semiarid Mu Us Desert ecosystems. Introducing leguminous plants and applying P fertiliser could alleviate this issue. In summary, A. ordosica increased SOC accumulation and nutrient availability in the fine scale (pedon scale) and improved the function of the Mu Us Desert ecosystem.
Data availability
All data generated or analysed during this study are included in this published article (and its supplementary information files).
Code availability
Not applicable.
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This research was supported by the National Natural Science Foundation of China (grant number 41877541, 41471222).
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Zhilong Lan and Jianguo Zhang designed the experiments. Xiong Li, Qiang Dong and Guangjun Fu developed the methodology. Zhilong Lan, Shaolei Zhang and Liangchen Xie performed the experiments and analysed the data. Zhilong Lan prepared the manuscript. Tanveer A. Sial, Abdu G. Shar, Jinglong Fan and Jianguo Zhang provided editorial advice.
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Lan, Z., Zhang, S., Xie, L. et al. Effects of Artemisia ordosica on fine-scale spatial distribution of soil C, N and P and physical–chemical properties in the Mu Us Desert, China. J Soils Sediments 22, 172–184 (2022). https://doi.org/10.1007/s11368-021-03067-x
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DOI: https://doi.org/10.1007/s11368-021-03067-x