Abstract
Vegetation gradients in Central Brazil encompass sharp transitions from savanna to forests, representing an iconic example of how interactions between plants and soils regulate biogeographical boundaries. Here we describe how canopy productivity regulates nutrient inputs to soils, affecting fertility and influencing ecosystem distribution. Based on soil and litter systematically collected during 12 months along a gallery forest-savanna transition, we determined associations between canopy cover (leaf area index—LAI) and the (re)cycling of essential macronutrients. This evaluation was aimed at aggregating information about biogeochemical controls of ecosystem distribution/productivity, to support conservation and management efforts in the region. We confirmed two hypotheses: (i) nutrient inputs via litterfall are significantly higher in forest than in adjacent savanna, and (ii) litter quality varies with canopy productivity and litter nutrient concentrations influence soil fertility reinforcing forest and savanna as alternate stable states. These observations delineate a productivity-efficiency tradeoff in which savannas communities are more efficient in the use of limiting nutrients, yet, less productive than forest communities. The relative importance of different nutrients, apparent on recovery rates in the litter regressed against LAI, revealed that the expansion of forest ecosystems is limited by P > Mg > K > N, with highest conservation observed for P. Differences in Ca input were also significant among ecosystems, but depended solely on the amount of deposited litter, with no differences in recovery rates observed between forests and savannas. A tradeoff-based framework could be used to predict ecotonal stability in the region, with transitions between savannas and forests marked by clear changes in species composition, productivity, litter deposition, and soil fertility.
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Introduction
In the cerrado region of Central Brazil, riparian forests and savannas, known as gallery forests and cerrado sensu stricto, respectively (Ribeiro and Walter 2008), occur side by side. Their transitions are characterized by abrupt changes in vegetation structure and many factors have been found to be associated with the long-term persistence of these sharp ecotones. For instance, groundwater availability (Eiten 1994; Rossatto et al. 2012, 2014), topography (Ruggiero et al. 2006), nutrient availability (Haridasan 1998; Silva et al. 2008, 2013), and fire frequency (Coutinho 2006; Hoffmann et al. 2012) are some of the main drivers of variations in vegetation physiognomies across the regional landscape. Since the strongest associations are found between vegetation structure and soil nutrient status (Furley 1992, 1999), it has been suggested that forest expansion or retraction result from vegetation-induced changes in soil fertility (Durigan and Ratter 2006; Furley 1999; Hopkins 1992; Ratter 1992; Silva and Anand 2011; Silva et al. 2008, 2010, 2013). These associations between soil and vegetation are also observed globally in other savanna ecosystems in Africa, Australia (Bond et al. 2004; Lehmann et al. 2011), and North America (Dahlgren et al. 2003). However, the mechanisms involved in this process remain poorly understood. Causal links between species performance, community composition, resource use, and nutrient (re)cycling at the ecosystem level remain to be established. Here we investigate how canopy production translates into nutrient depositional gradients along a sharp forest-savanna transition. Forest encroachment into ancient savanna and grassland ecosystems has been observed throughout South America (Franco et al. 2014; Silva 2014; Silva and Anand 2013), and represents a new challenge for developing sound management practices and conservation policies in the region.
Gallery forests cover approximately 5 % of the cerrado region, which encompasses approximately 2,000,000 km2 (Ribeiro and Walter 2008). These forests follow streambeds and rivers as corridors throughout the landscape and typically have greater soil water and nutrient availability than the neighboring savanna (Haridasan 1998). Gallery forests woody vegetation is mostly evergreen (Ribeiro and Walter 2008) while some savanna tree species lose leaves seasonally (Franco et al. 2005). Gallery forests also have higher productivity than savannas, as measured, for example, by total vegetation cover per land area (leaf area index—LAI; Silva et al. 2008, 2013), suppressing virtually all flammable grass cover that dominates open savannas (Hoffmann et al. 2005, 2012; Silva et al. 2013). Savanna and forest tree species represent different functional types, as tradeoffs between nutrient requirements and adaptations to resist fires reinforce savanna and forest as alternate stable states and explain the long-term persistence of vegetation mosaics in this region (Silva et al. 2013). Woody savanna species are adapted to low fertility conditions that predominate in the region (Haridasan 2008), whereas only a small group of forest species colonizes open areas leading to forest expansion (Geiger et al. 2011).
Current features of gallery forests and savannas such as soil organic matter content, litter production, and nutrients concentrations in soil and litter are important to understand how vegetation and soils interact to regulate boundary dynamics. Low nutrient concentrations are generally observed in litter produced under low soil nutrient availability (Vitousek 2004) (i.e., savannas on highly weathered oxisols), whereas the opposite is expected for more fertile sites, where individual plants and entire ecosystems tend to be less efficient, depositing more nutrients as litter per unit of biomass produced (Parron 2004; Parron et al. 2010; Silva and Anand 2011). Litter production and nutrient inputs have been previously evaluated in savanna (Kozovits et al. 2007; Nardoto et al. 2006; Peres et al. 1983; Schiavini 1983; Silva 2004; Silva et al. 2007; Villalobos-Vega et al. 2011) and forest communities (Parron 2004; Parron et al. 2010). Combined, these studies suggest the existence of a tradeoff between productivity and efficiency at the ecosystem level, which remains to be demonstrated.
Here, we investigate litter production and soil fertility as a way of characterizing nutrient dynamics across a typical gallery forest and savanna transition in Central Brazil, using a new approach to interpret litterfall and nutrient deposition as a proxy for nutrient use efficiency at the ecosystem level. Specifically, we tested two hypotheses: (i) variation in canopy productivity across the transition leads to marked differences in total nutrient input to savannas and forests, greater in the latter; and (ii) differences in nutrient use efficiency affect litter quality and influence soil fertility across the transition reinforcing the co-existence forest and savanna as alternate stable states. We analyzed litter and soil macronutrients across a natural (fire-protected) gradient spanning forest-savanna transitions, discussing the role of nutrients in influencing species composition and ecosystem productivity, which will be important to support conservation and management efforts in the region and elsewhere.
Methods
Site description
This study was performed at the Ecological Reserve of the Brazilian Institute of Geography and Statistics (IBGE) located at 35 km south from Brasilia in the Federal District of Brazil (15°57′05″–15°57′15″ S and 47°52′38″–47°52′54″ W), at an elevation of 1100 m a.s.l. and nearly flat terrains (5° slope), along forest-savanna transitions (Fig. 1a). The average annual rainfall recorded at IBGE meteorological station is 1461 mm (1980–2008), with a well-defined dry season from May to September and a mean annual temperature of 22.5 °C. At the time this study was performed, from October 2007 to September 2008, total rainfall was 1635 mm. Designated as a conservation area in 1975, the reserve harbors an area of 1350 hectares protecting different vegetation physiognomies, such as grasslands (open fields known as campo limpo or campo sujo), predominant savanna (cerrado sensu stricto), woodland savanna or dense wooded savannas (cerradão), and gallery forests associated with streams and springs (Furley 2007; Ribeiro and Walter 2008; Walter et al. 2008). Soils at the reserve are well characterized and deep well-drained oxisols predominate. The chosen area for this study was the gallery forest-savanna boundary of the Roncador stream (Fig. 1b), which was disturbed by fire only once in the past 35 years operation, in 1994, when non-prescribed burning occurred. The vegetation has recovered to its original state and there is now a clear (few meters) transition in vegetation structure and composition across this boundary, with a decline of 53 % of leaf area index (LAI) from gallery forest to savanna (Geiger et al. 2011). In total, 129 tree species from 51 botanical families occur along this transition (Geiger et al. 2011; Supplementary Material), including species of the family Fabaceae (Faboideae), typical N2-fixing species, among the most abundant in both forest and savanna (Felfili et al. 2004). This type of abrupt forest-savanna transition is commonly observed throughout the region, even in fire-protected areas, representing an integral component of the landscape.
Sampling design
Four transects of 60 m length separated by a distance of 40 m from each other were delineated according to the vegetation gradient at the Roncador site, so that they were positioned perpendicularly to the forest edge and centered at the border. The central point of each transect is defined as 0 (zero), where an unambiguous interruption of the forest canopy and the decreasing tree density coincide with the appearing of shrubs and grasses. From this central point, the four transects extended 30 m into forest and 30 m into savanna (Fig. 1c).
Litter traps of 0.25 m2 and 2.0 mm nylon mesh were arranged every 5 m along each transect (Fig. 1c). All traps were placed 15 cm above the ground. Since our goal was to evaluate changes in overstory litterfall and composition, litter from ground layer grasses and small eudicot species bellow 15 cm height were not included. In terms of mass and nutrient stock, the contribution of the herbaceous layer in both forest and savanna represents only a small fraction of total litter (Abdala et al. 1998), here better represented by the combination of contributions from the tree and shrub components. Over an entire year, paper bags were used to collect the litter intercepted and accumulated by each litter trap every 15 days. These samples were combined to obtain monthly samples. After sorting into two fractions: leaves and miscellaneous (leaf fragments, reproductive parts, and twigs with diameter ≤1 cm), the samples were washed using distilled water, oven-dried in paper bags at 70 °C for 72 h, and then weighed. Branches and wood debris greater than 1 cm diameter and occasional animal tissues were discarded.
Soil samples of 100 g were collected using a drill, along each transect immediately adjacent to each litter trap (Fig. 1c). Soils were sampled once at the end of the dry season (September 2008), from 0 to 10 cm and from 10 to 20 cm depths, totaling 52 samples per depth. Soil samples were sieved through a 2.0 mm mesh to separate fine roots, placed in plastic bags, and dried at room temperature.
Also adjacent to litter traps, LAI was obtained every 5 m along the four transects (Fig. 1c) using the CI-110 Plant Canopy Imager (CID Inc., Camas, WA, USA). This instrument captures LAI indirectly, based on digital hemispherical images (150° field of view) of the vegetation overhead to estimate canopy LAI as a measure of total vegetation cover per land area (vegetation density quantification rather than green leaf area per se). At each point of transects, images were taken at 30 cm above ground level in order to measure trees and shrubs LAI, excluding herbaceous layer. Images were taken in May 2008 under dawn conditions at the end of rainy season, when all species even deciduous ones had full foliage. For trees, our values included stem area, as well as leaf area.
Sample analysis
Samples obtained every fifteen days in the litter traps positioned along the four transects were combined to obtain monthly samples, totaling 52 samples per month (26 of leaves and 26 of miscellaneous). Chemical analyses of litter correspond to the leaf fraction (leaf litterfall), which was powdered and homogenized after being dried. Total N concentration in leaves was determined by the micro-Kjeldahl digestion and distillation method (Bremner and Mulvaney 1982), while P, K, Ca, and Mg concentrations were determined by digestion with nitric, sulfuric, and perchloric tri-acid solution (10:1:2). The cations were determined with atomic absorption spectrophotometry and available P by colorimetry (Allen 1989).
Soil organic carbon was determined using the Walkley and Black method (Walkley and Black 1934) and total N was determined by micro-Kjeldahl digestion and distillation method (Bremner and Mulvaney 1982). Exchangeable calcium and magnesium were extracted by a 1 M KCl solution and available P and K were determined using the method of Mehlich (Mehlich 1953). The cations concentrations in soils were determined with atomic absorption spectrophotometry and available P by colorimetry (Allen 1989). Soil physics data were not included in our analysis, since differences of soil texture (i.e., clay) along forest and savanna transitions at the IBGE Reserve are not crucial to determine the distribution of both vegetation types (Hoffmann et al. 2009, 2012).
Statistical analysis
Total nutrient input was calculated by multiplying leaf litterfall mass by the concentration of macronutrients at every sampling point along the transition. We performed repeated measures analyses of variance, using a mixed effect model to test the significance of fixed (distance from the border) and random (date) effects on leaves and miscellaneous, leaf litterfall nutrients, and soil nutrient concentrations. We tested fixed and random effects, using interaction terms to examine the significance of variations in litter deposition and nutrient input along the gradient at different sampling dates. Data were log transformed prior to analysis to meet assumptions of normality (Shapiro-Wilks test) (Shapiro and Wilk 1965) and homogeneity of variance, detected as departures from homoscedasticity (Bartlett’s test), both at <0.05 significance levels. Regression analyses were performed and angular coefficients of least square regressions used to interpret changes in canopy productivity (total annual leaf litterfall mass) and recovery of each measured macronutrient (dependent variables), separately regressed against LAI values across the transition. As such, litterfall mass and nutrient deposition could be interpreted as a proxy for plants and ecosystems efficiency and recycling patterns. All statistical analyses were performed using the SAS-created JMP statistical software (SAS Institute 2012).
Results
Leaf area index of trees and shrubs increased from savanna toward forest, with values ranging from 1.4 to 3.9, where the lowest values were found at 20 m from border into the savanna and the highest values measured at 30 m from border into the forest. Measurements of LAI characterized the differences in canopy productivity and vegetation structure across the transition, represented as the ratio of upper leaf surface per unit of land surface area (Fig. 2a).
Consistent with changes in vegetation structure, total annual litterfall was significantly higher in the forest physiognomy (6.3 t ha−1 year−1) than in the savanna (3.5 t ha−1 year−1) (Fig. 2b). The leaf fraction accounted for approximately 75 % of total litterfall, whereas the remaining fraction was classified as miscellaneous. The outputs of a repeated measures analysis showed highly significant (p < 0.01) effects of both date and distance from the border, in which the forest environment received in average 4.7 t ha−1 of leaves and 1.6 t ha−1 of miscellaneous litter throughout the year. No significant interactions occurred between leaf litter input along the gradient and sampling date (p = 0.59), but significant interactions (p = 0.01) occurred between miscellaneous litter input and time. In both cases, most of the litter input occurred between the months of August and September, which corresponds to the dry season. However, during the annual production of the miscellaneous component, it was observed an increase in the amount of twigs, wood debris, flowers, fruits, and seeds fragments due to the impact of the first rainfalls after the prolonged dry season (data not shown).
The total input of macronutrients from leaf litter along the forest-savanna transects followed the same trend as observed for the mass of leaves and miscellaneous produced across the transition, with all nutrients analyzed showing higher inputs in litter produced by the forest ecosystem (Fig. 3b). Only Ca concentration in leaf litterfall between savanna and forest was significantly different (p < 0.05), with higher values found in the forested habitat (Fig. 3a).
Using least square regressions using the total annual leaf litterfall against LAI, we found a strong positive relationship (R 2 = 0.88, p < 0.01), with an increase of approximately 255.54 kg of leaf litter per ha−1 year−1 for each unit increased in LAI measured during the rainy season (Fig. 2a). We used this relationship as a baseline for comparisons with least square regressions performed using litter nutrient inputs, by comparing the angular coefficients of each of these linear regressions as a proxy for nutrient use efficiency; i.e., biomass produced per unit of each nutrient recovered. In this sense, the angular coefficient of each regression (Fig. 4) was interpreted as an indicator of the degree of conservative use of each nutrient. The regression line closest to that of litterfall mass corresponds to the least conservative scenario. In this scenario, litter mass explains most of the variation in nutrient input, while the most conservative scenario (i.e., where nutrients are recycled within plants and variations in litter deposition do not affect input) would be represented by low angular coefficients (i.e., flat lines; Fig. 4).
The results of these regressions revealed highly significant relationships between nutrient inputs and LAI (R 2 > 0.82) with slope values of 0.12 (P), 0.51 (Mg), 1.99 (K), 2.67 (N), and 4.35 (Ca), indicating the following sequence P > Mg > K > N as representative of high to low conservation, corresponding to differences in efficiency caused by distinct limitation, where P is the most limiting nutrient. Importantly, levels of deposited Ca cannot be compared directly with other nutrients through this method, as it is a structural component of the plant tissue that is not recycled (i.e., reassimilated) prior to litter deposition. This is evidenced by the large angular coefficient of Ca deposition, which closely matches total litterfall production along the gradient, even though Ca is considered a major limiting nutrient for biomass production and forest expansion in the region (Silva et al. 2013).
Soil organic carbon and total N varied significantly across the gradient with an abrupt change at the border and consistently higher values observed in the forest than in the adjacent savanna (Fig. 5). Reinforcing the notion of a P-limited transition, available P levels showed no significant changes across the gradient, ranging from 0.77 to 1.35 mg kg−1 (0 to 10 cm depth) and from 0.77 to 1.15 mg kg−1 (10 to 20 cm depth). Similarly, levels of K were not significantly different across the gradient, ranging from 0.18 to 0.30 cmol (+) kg−1 (0 to 10 cm depth) and from 0.16 to 0.23 cmol (+) kg−1 (10 to 20 cm depth). Exchangeable Ca and Mg concentrations at both soil depths showed divergent patterns. Levels of Ca were found to be depleted at the border, but reached maximum values 30 m deep into the savanna for both soil layers (5.86 and 2.75 cmol (+) kg−1); and at 25 m (7.88 cmol (+) kg−1, 0 to 10 cm depth) and at 20 m (4.44 cmol (+) kg−1, 10 to 20 cm depth) into the forest. Levels of Mg peaked at the border in both soil layers (1.88 and 1.23 cmol (+) kg−1) (Fig. 5), but no relationship was found between soil and leaf litterfall nutrient concentrations along the forest and savanna transition (Supplementary Material).
Discussion
Litter deposition across forest-savanna boundaries
Our results confirmed the hypothesis that an increase in litter production from savanna to forest determines differences in total nutrient input. Inputs of N, P, K, Ca, and Mg significantly increase from open to closed canopies, mostly due to the effect of leaf litter mass, but also determining changes in litter quality across the forest-savanna transition. This corroborates the second hypothesis that differences in nutrient use efficiency and litter quality promote higher fertility in forest soils. A more in-depth analysis reveals that nutrient inputs vary along the gradient, with the influx of different macronutrients reflecting the degree in which each element limits productivity. These results show that patterns previously identified at the individual and species levels also hold at the ecosystem level, delineating a productivity-efficiency tradeoff, in which savannas communities are more efficient in the use of limiting nutrients, yet, less productive than forest communities. Under similar edaphic conditions, forest species grow faster and have higher leaf nutrient concentration than their congeneric savanna counterparts (Hoffmann et al. 2005; Rossatto et al. 2009). This is attributed to differences in stem to crown biomass allocation, which characterize forest and savanna trees as separate functional groups (Silva et al. 2013) and emphasize the role of species traits in generating gradients of resource accumulation through litter deposition.
Previous studies attempted to quantify the amount of litterfall produced in forest ecosystems of Central Brazil. At the same reserve, Parron (2004) registered an average of 6.7 t ha−1 year−1 for three gallery forest communities. These values are similar to ours (6.3 t ha−1 year−1), but litterfall in other forests under constraining moisture regime, range from 11.1 t ha−1 to 8.8 t ha−1 (Pagano and Durigan 2000), whereas in woodland savanna (cerradão) of the same region, annual litterfall has been reported to be 7.8 t ha−1 (Peres et al. 1983) and 5.6 t ha−1 (Cianciaruso et al. 2006). In regional deciduous forests litterfall ranges from values close to ours (6.1 t ha−1 year−1; Werneck et al. 2001) to almost twice as large (12.2 t ha−1 year−1; Hora et al. 2008). Notably, in all these cases litterfall produced by the canopies of forest species surpassed values that we observed in open savanna (3.5 t ha−1 year−1). This pronounced difference in total litterfall happens within a few meters from the forest border and makes the total amount of nutrients derived from litter to the soil surface much lower than values observed in forests. Other surveys in savannas areas ranged from 1.5 to 2.4 t ha−1 year−1 (Kozovits et al. 2007; Nardoto et al. 2006; Peres et al. 1983; Schiavini 1983), indicating an even lower contribution from litter inputs.
Differences in environmental conditions may also account for oscillations in litter deposition throughout the year (Peres et al. 1983). Seasonal water shortage, for instance, provokes greater shed of vegetative material in savannas (Dias and Oliveira-Filho 1997), while continuous water supplies in gallery forests buffers the effects of seasonality. Beyond such effects, phenology of the dominant species across the vegetation transition could be a potential cause for differences in seasonal litter production and nutrient input (Marimon-Júnior 2007).
Across the transition, the most important differences in soils were organic carbon and N contents. This contrasts with patterns of litter nutrient composition and indicates that nutrient fluxes via litterfall are not simply a function of soil fertility. Instead, they reflect the large differences in nutrient accumulation in forest and savanna vegetation, evidenced in the much greater aboveground biomass and nutrient stocks of forests (Silva et al. 2013). When litter quality is low, such as observed in the savannas, decomposition is expected to take longer, causing a lag in nutrient release and constraining both soil fertility and plant productivity (Dahlgren et al. 2003; Vitousek 2004). Here, corroborating this expectation, total nutrient inputs were found to be positively associated with canopy productivity, but there are important differences concerning each nutrient. The following sequence represents the order of limiting nutrients according to their degree of conservation (P > Mg > K > N), where P was the element used most conservatively across the vegetation gradient. Despite close associations between N and P levels in plant across biomes (Elser et al. 2007), our results show large N fluxes via leaf litterfall in contrast with low P fluxes as LAI increases, indicating a high P use efficiency.
As mentioned above, Ca cannot be compared directly with other nutrients based on its relationship with LAI, due to its structural role in plant cell wall (Lambers et al. 1998), which does not permit reassimilation prior to litter deposition (Vergutz et al. 2012). However, Ca is considered a major limiting nutrient for biomass production and forest expansion in the region (Silva et al. 2013). Due to its low mobility within plants (Kazakou et al. 2007), Ca input from litter is dependent solely on the total amount of deposited residues (König et al. 2002), which would be enhanced in forest soils as a result of greater canopy productivity and, consequently, mass of litter deposited (Fig. 4).
The role of litter nutrient inputs in driving forest expansion
Based on both individual level measurements of congeneric savanna and forest tree and estimates of soil and biomass nutrient stocks, Silva et al. (2013) concluded that Ca and P are the most limiting nutrients to build high-biomass forests in forest-savanna transitions. In fact, these nutrients are the most limiting ones to support a transition to any forested state and appear to be higher in the aboveground biomass than in soils of savannas and gallery forests. This is supported by our findings that around the border there is a decline in soil Ca availability (Fig. 5), which suggests a substantial constraint to forest expansion. The amount of Ca required to build low (woodland savanna) and high (gallery) biomass forests vary from 46 to 645 kg ha−1, whereas P requirements range from 3 to 57 kg ha−1 to allow the canopy cover typical of forest ecosystems found in the region (Silva et al. 2013). The slight variation of P concentrations in litter across the gradient (Fig. 3a), in spite of large variations in litter deposition, corroborates other studies using data collected from hundreds of surveys analyzing the response of diverse terrestrial ecosystems to P enrichment, considered particularly limiting in tropical ecosystems (Elser et al. 2007).
Soil stocks of N, K, and Mg are sufficient to allow forest cover at the study site, but the addition of P and Ca from litter would be required to allow the establishment and development of forest communities (Silva et al. 2013), favoring a transition to stable forested states (Hoffmann et al. 2009). The observed variation in litter nutrient content in relation to canopy production according to their respective degree of limitation has two important implications: (i) more litter would mean more nutrient inputs to the system except in the case of P which is recycled most efficiently; and (ii) soil nutrient gradients would be reinforced by vegetation with the most important nutrient determining forest distribution as a result of soil-plant feedbacks being Ca. Since Ca, as well as P, K, and Mg are originated from the parent material (Brady and Weil 2007), preexisting differences between organic nutrients (i.e., N) in the highly weathered soils typical of the studied region would also be important in determining the potential for forest expansion.
Implications for conservation and management
The continuous input of nutrient derived from litter is the main nutrients source maintaining gallery forest’s structure and species diversity. The fire-protected area of the Roncador vegetation boundary currently has forest species comprising 14 % of mature species and more than 33 % of juveniles (seedlings and saplings) in the adjacent savanna community, which supports the notion that floristic shifts precede structural changes (Geiger et al. 2011). Because of greater nutrient investments in leaf canopy and stem biomass, forest species differ from savanna species when both are growing under nutrient-poor soils. Intrinsic species differences lead to differences at the ecosystem level (Silva et al. 2013), determining new suppression and resistance thresholds (Hoffmann et al. 2012), which increase the possibility of forest encroachment (Hoffmann et al. 2003). Causal links between efficiency-productivity tradeoffs at the species level and community dynamics can influence ecosystem responses to environmental change at large spatiotemporal scales, as, for example, when refugia populations expand or contract in response to climate fluctuations (Silva and Anand 2011, 2013). Similarly, we provide evidence that a tradeoff-based approach is useful to explain ecosystem dynamics and ecotonal stability, with transitions between savanna and gallery forest marked by measurable changes in litter and soil composition. Long-term assessments and fertilization experiments would be useful in validating the generality of these patterns and to guide conservation and management efforts based on nutritional controls of species performance, as it propagates to communities and ecosystems.
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Acknowledgments
We thank the staff of IBGE Ecological Reserve for infrastructure and logistical support. We also thank William Horwath for valuable discussion, William Hoffmann, Augusto Franco, Erika Geiger, Sybil Gotsh, and many other colleagues who helped with data collection, and Mara Rúbia Suzana Chaves who helped with laboratory analysis. This research was supported by CAPES Brazil.
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Communicated by Erik P Hamerlynck.
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Paiva, A.O., Silva, L.C.R. & Haridasan, M. Productivity-efficiency tradeoffs in tropical gallery forest-savanna transitions: linking plant and soil processes through litter input and composition. Plant Ecol 216, 775–787 (2015). https://doi.org/10.1007/s11258-015-0466-8
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DOI: https://doi.org/10.1007/s11258-015-0466-8