Abstract
Aiming at creation of the chemical basis for serotyping of strains of Yersinia spp., we studied the O-specific polysaccharide (OPS) of Yersinia frederiksenii H56-36/81 belonging to serotype O:60. A branched polysaccharide was obtained by mild alkaline degradation of the lipopolysaccharide (LPS) isolated from bacterial cells by the phenol—water extraction and found to contain d-ribose, l-rhamnose, and 4-deoxy d-arabino-hexose (d-ara4dHex). The last monosaccharide occurs rarely in nature and earlier has been found only in OPS of Citrobacter spp. Mild acid hydrolysis of the LPS resulted in cleavage of the d-ara4dHex side-chains to give a linear polysaccharide. Structure of the hexasaccharide repeating unit of the OPS was established by studies of both isolated polysaccharides by 1D and 2D 1H and 13C NMR spectroscopy.
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Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 6, pp. 1625—1629, June, 2016.
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Sizova, O.V., Shashkov, A.S., Shaikhutdinova, R.Z. et al. Structure of the O-polysaccharide of Yersinia frederiksenii H56-36/81 (serotype O:60) containing 4-deoxy-d-arabino-hexose. Russ Chem Bull 65, 1625–1629 (2016). https://doi.org/10.1007/s11172-016-1490-0
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DOI: https://doi.org/10.1007/s11172-016-1490-0