Abstract
Prolonged exposure to abiotic stresses causes oxidative stress, which affects plant development and survival. In this research, the overexpression of ZmARF1 improved tolerance to low Pi, drought and salinity stresses. The transgenic plants manifested tolerance to low Pi by their superior root phenotypic traits: root length, root tips, root surface area, and root volume, compared to wide-type (WT) plants. Moreover, the transgenic plants exhibited higher root and leaf Pi content and upregulated the high affinity Pi transporters PHT1;2 and phosphorus starvation inducing (PSI) genes PHO2 and PHR1 under low Pi conditions. Transgenic Arabidopsis displayed tolerance to drought and salt stress by maintaining higher chlorophyll content and chlorophyll fluorescence, lower water loss rates, and ion leakage, which contributed to the survival of overexpression lines compared to the WT. Transcriptome profiling identified a peroxidase gene, POX, whose transcript was upregulated by these abiotic stresses. Furthermore, we confirmed that ZmARF1 bound to the auxin response element (AuxRE) in the promoter of POX and enhanced its transcription to mediate tolerance to oxidative stress imposed by low Pi, drought and salt stress in the transgenic seedlings. These results demonstrate that ZmARF1 has significant potential for improving the tolerance of crops to multiple abiotic stresses.
Key message
ZmARF1 is a pleiotropic gene which confers tolerance to multiple abiotic stresses in ZmARF1-OE transgenic Arabidopsis as revealed by stress-response phenotypes of the transgenic lines relative to WT. ZmARF1 confers stress tolerance through a mechanism involving its association with an antioxidant enzyme, AtPOX, whose expression was simultaneously up regulated by low Pi, drought and salt stresses, whiles its promoter activity was significantly activated by ZmARF1.
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Introduction
Plants, as sessile organisms, are predominantly exposed to numerous abiotic stresses during their growth cycles, such as extreme temperatures, nutrient deficiencies, drought, salinity, and metal toxicity, which pose a serious threat on global food security (Dixit et al. 2018). Abiotic stresses induce major constraints on crop productivity and have become one of the leading limitations to maximizing crop yields globally, causing up to an average crop yield loss of up to 50% (Pandey et al. 2017). The limitations imposed by environmental stresses on crop productivity are expected to worsen because of changes in global climate. The effect of global climate change are projected to decrease global yields of major staple crops such as maize, rice, wheat, and soybean by 3–7% per °C rise in temperature (Zhao et al. 2017). Moreover, with the global population projected to reach 10 billion by 2050 (Rockstrom et al. 2017), competition for arable land for non-agricultural activities, will push agricultural production into environments less suited for crop cultivation (Bajaj & Mohanty 2005; Miao et al. 2016). Consequently, the development of stress-tolerant crops has become imperative to sustaining yield stability under adverse environmental conditions to minimize the impact of environmental stress on crop production (Zhang et al. 2018).
Drought stress, or water deficit, is one of the major abiotic stresses that have significantly limited crop survival, development, and yields worldwide over the last few decades (Wang et al. 2019). An estimated 1,820 million Mg of cereal (maize, rice, and wheat) production has been lost globally due to drought stress alone over the past four decades (Lesk et al. 2016). Drought stress is projected to impose major limitations on the productivity of more than half of the world’s arable lands in the next 50 years (Dhankher & Foyer 2018). Both drought and salinity stresses decrease the availability of water to plant cells by inducing lower water potential, which inhibits overall crop yield and quality. Salt stress causes ion imbalance and disrupts nutrient acquisition due to osmotic stress and Na+ toxicity, consequently inhibiting the normal physiological functioning of plants (Nan et al. 2018). Salt stress also stimulates swift the production and accumulation of reactive oxygen species (ROS) such as superoxides (O2−) and hydrogen peroxide (H2O2), as secondary stress responses, which are extremely toxic to plants (Mittler 2017; Yang & Guo 2018). Approximately 20% of global arable lands are affected by salt stress, a figure projected to increase in the immediate future due to climate change and anthropogenic activities (Shrivastava & Kumar 2015). Additionally, phosphate (Pi) is a key constituent of important cellular molecules such as ATP, nucleic acids, and phospholipids, and participates in numerous biochemical pathways such as energy transmission, gene expression and signal transduction. Pi deficiency limits plant growth and development, and remains one of the main challenges affecting crop productivity (Chen et al. 2018). An estimated 70% of global arable lands are reported to contain suboptimal Pi concentration, which restricts optimal plant growth and development (Herrera-Estrella & Lopez-Arredondo 2016). The reduction in crop yield and productivity occasioned by these abiotic stresses could be mitigated by developing broad-spectrum abiotic stress-tolerant plants that can yield better on lands affected by these stress conditions. However, the mechanisms of gene–gene networks governing tolerance to multiple abiotic stresses are complex and not well understood.
The auxin response factor (ARF) family proteins are a group of highly conserved plant specific transcription factors that play key regulatory roles in the expression of auxin-responsive genes (Liscum & Reed 2002). To date, numerous ARFs have been described in various plant species, such as Arabidopsis thaliana (Guilfoyle & Hagen 2007), Oryza sativa (Wang et al. 2007), Zea mays (Wang et al. 2012) and Musa acuminata (Hu et al. 2015). Besides mediating several aspects of plant growth and development, ARF proteins have been reported to participate in plant response to multiple abiotic stresses (Bouzroud et al. 2020, 2018; Chen et al. 2019; Song et al. 2023). The molecular mechanism by which auxin controls gene expression occurs either through canonical or non-canonical signaling pathways. The canonical pathway, also called the nuclear auxin pathway (NAP), involves three families of proteins: the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX (TIR1/AFB) family, which acts as the auxin receptor, AUXIN/INDOLE 3-ACETIC ACID INDUCIBLE (Aux/IAA) repressor proteins, and ARF transcription factors (Kong et al. 2016; Wright & Nemhauser 2015). When auxin is low or absent, Aux/IAA recruits the transcription co-repressor TOPLESS/TOPLESS-RELATED (TPL/TPR) and interacts with ARF transcription factors through the shared PB1 domains, thus repressing gene transcription and auxin responses (Szemenyei et al. 2008). In the presence of auxin, auxin binds TIR1/AFBs and increases their affinity toward Aux/IAA transcriptional repressors to facilitate their proteasome-dependent degradation by targeting them for ubiquitination, thereby relieving the repression of ARF transcription factors to regulate the expression of auxin-responsive genes (Dharmasiri et al. 2005; Kepinski & Leyser 2005). The non-canonical auxin pathway can occur through two distinct routes. The first pathway involves the receptor-like kinase TRANSMEMBRANE KINASE 1 (TMK1), which interacts with AUXIN BINDING PROTEIN 1 (ABP1) and activates Rho-like guanosine triphosphatases (GTPase) from plants (ROP2/ROP6) in an auxin-dependent manner (Xu et al. 2014). Moreover, after auxin sensing, TMK1 phosphorylates and stabilizes IAA32 and IAA34, thus regulating ARF transcription factors (Cao et al. 2019). Alternatively, auxin binding to the atypical ARF3/ETTIN(ETT) modulates chromatin and interacts with other transcriptional regulators (Simonini et al. 2016). Direct auxin binding to ARF3 leads to its release from co-repressors of the TOPLESS/TOPLESS-RELATED family, resulting in histone acetylation and induction of gene expression, creating a reversible switch between repressive and de-repressive chromatin states (Kuhn et al. 2020; Liu et al., 2024). Constitutive expression of ARF genes in various plant species modulates tolerance to environmental stresses such as drought, cold, salinity and low Pi stress (Jain & Khurana 2009; Shen et al. 2013). For instance, overexpression of a sweet potato auxin response factor (IbARF5) modulated carotenoid biosynthesis and conferred salt and drought tolerance in transgenic Arabidopsis (Kang et al. 2018). Overexpression of ZmARF4 conferred low Pi stress tolerance in Arabidopsis by enhancing lateral root development, Pi mobilization and expression of Pi responsive gene AtANS1 (Li et al. 2022).
Under the natural growing environment of plants, they encounter two or more stresses that affect their survival and limit their productivity. Researchers have outlined different approaches to achieving tolerance to multiple stresses in plants. It has been outlined that tolerance to multiple stresses can be achieved in plants by the gene pyramid approach that involves concurrent expression of multiple genes (Hema et al. 2014; Hong et al. 2016). In this study, we demonstrated that overexpression of ZmARF1 conferred tolerance to low Pi, drought, and salt stresses in transgenic Arabidopsis compared to wild-type (WT). Low Pi tolerance in ZmARF1-transgenic Arabidopsis was manifested by better root phenotypic traits, higher Pi mobilization and upregulation of Pi stress inducible genes under low Pi conditions. Meanwhile, transgenic Arabidopsis manifested tolerance to drought and salt stress by maintaining lower ion leakage and water loss rates, higher chlorophyll parameters and survival rates compared to WT respectively. Transcriptome profiling identified POX, a member of the peroxidase superfamily, whose transcript level was upregulated under low Pi, drought, and salt stresses. Furthermore, ZmARF1 bound to the AuxRE in the promoter of POX and enhanced its transcription, and this interaction is believed to have contributed to conferring tolerance to oxidative stress imposed by low Pi, drought and salt stress in the transgenic seedlings.
Materials and methods
Plant material and stress treatment
The Arabidopsis thaliana WT plants were of the Columbia-0 (Col-0) ecotype. Seeds of the WT and ZmARF1 overexpressing lines were surface sterilized with 75% (v/v) ethanol and 5% (v/v) NaOCl followed by five times rinse with sterilized distilled water as outlined by Hu et al. (2023). Seeds were subsequently placed on P-sufficient (HP) 1/2 MS medium and cold treated at 4 °C for 2 days. The 1/2 MS medium plates were then transferred to a growth chamber and kept upright at 22 °C and a 16 h light/8 h dark photoperiod for 7 days. Uniform seedlings were then transferred to P-sufficient (HP) and P-deficient (LP) 1/2 MS medium and cultured for 7 days. HP condition was made up of 1/2 MS medium (Phytotech, Cat#M519) with 5% (w/v) sucrose and 0.6% (w/v) agar powder (Solarbio, Cat#A8190). LP condition was made up of 1/2 MS without phosphate (Caisson Lab. REF: MSP11-10LT) with 5% (w/v) sucrose and 0.6% (w/v) agar powder, supplemented with HP (100:1). Root phenotypic traits, Pi accumulation and expression of Pi stress inducible genes were determined after HP and LP treatments.
For salt stress treatment, WT and transgenic seeds germinated on 1/2 MS medium were transplanted to pots containing equal volumes of peat soil and grown in growth chamber for 5 weeks at 22 °C and a 16 h light/8 h dark condition. Salt stress was imposed on seedlings by irrigating with 200 mM NaCl solution at 3 days interval for two weeks. Control treatment was made up of irrigation with water. For natural drought treatment, WT and transgenic seeds germinated on 1/2 MS medium were transplanted to pots containing equal volumes of peat soil and grown in growth chamber for 3 weeks at 22 °C and a 16 h light/8 h dark condition. Pots were fully watered for 3 weeks and then water was withdrawn for 10 days for drought treatment, whiles control plants were continuously watered. After drought treatment, the seedlings were rehydrated for 3 days. Moreover, rosette leaves under drought and salt stress, alongside the respective control treatments, were sampled for physiological measurements. The phenotypic changes and survival rates among the WT and transgenic lines under drought stress and normal conditions were recorded.
Plasmid construction and plant transformation
The full coding sequence (CDS) of ZmARF1 (without the stop codon) was amplified by PCR from the maize P178 inbred line and cloned into the BamH I and Spe I sites of the pCAMBIA2300 expression vector, driven by the cauliflower mosaic virus (CaMV) 35S promoter, with a GFP tag at the C terminus. Gene-specific primers used are stated in Table S1. The pCAMBIA2300-ZmARF1-GFP construct was confirmed by sequencing and transformed into Arabidopsis (Col-0) ecotype by the Agrobacterium tumefaciens (strain GV3101) mediated transformation using the floral dip method (Clough & Bent 1998). T0 seeds were harvested and screened on 1/2 MS medium supplemented with 50 μg ml−1 of G418, resistant seedlings were transplanted into peat soil for selfing. T1 seeds were harvested and screened on 1/2 MS medium containing 50 μg ml−1 of G418 antibiotic for separation ratio identification. Approximately, 100 T3 seeds of independent transgenic lines and WT were screened on half MS strength medium supplemented with 50 μg ml−1 of G418, homozygosity was accessed based on 100% germination, root growth and survival. T3 homozygous positive transgenic lines overexpressing ZmARF1 were selected for subsequent experiments.
RNA extraction and RT-qPCR
Total RNA was extracted from seedlings using the Trizol kit (Invitrogen, CA, USA) according to the manufacturer’s protocol. According to the manufacturer’s instructions of reagent kit for operation of mRNA reverse transcription (Vazyme Biotech, Nanjing, China) and qRT-PCR (TransGen, Beijing, China), respectively. The expression of the housekeeping AtActin was used as an internal control. qRT-PCR was performed in three technical replicates. Specific primers used are listed in Table S1.
Expression pattern of ZmARF1 in response to Pi stress
Responsiveness of ZmARF1 to Pi stress was evaluated through RT-qPCR and western blotting assay. RT-qPCR was performed as described above. Total protein was extracted from the wild-type (Col-0) and ZmARF1 overexpression seedlings (OE8# and OE17#) after high and low Pi treatments using the HEPES cell lysis buffer (1 M HEPES, 2 M KCl, 500 mM EDTA, 20% Triton X-100,1 mM PMSF). Seedlings were ground into fine powder in liquid nitrogen and approximately 0.05 g was homogenized in 100 μl of HEPES cell lysis buffer. The protein extract (10 μl) was separated by gel electrophoresis on 10% SDS–polyacrylamide gel and transferred to a polyvinylidene difluoride membrane (Bio-rad). The membranes were subjected to immunoblotting with a primary anti-GFP antibody (Sangon) overnight and a secondary anti-rabbit antibody (Sangon) for 2 h. Proteins were visualized using an enhanced chemiluminescence kit (Thermo Fisher Scientific).
Measurement of root phenotypic traits
The root traits of WT and ZmARF1-overexpresing seedlings were captured by a scanner (Epson expression 10000XL, Epson Corporation, Japan) and analyzed with WinRHIZO software (WinRHIZO Pro 2008a) after HP and LP treatments. The root traits, including root length, root surface area, root volume and root tips were measured. Means were represented in bar graphs using the GraphPad Prism 8.0.1v software.
Measurement of Pi content
Phosphate content was determined according to previous study (Chiou et al. 2006) with slight modification. Fresh samples were ground into fine powder in liquid nitrogen. Approximately 0.1 g of powdered sample was dissolved in 400 μl of extraction buffer [10 mM Tris–HCl, 1 mM EDTA, 10 mM NaCl, 1 mM Beta-ME, and 1 mM PMSF, pH 8.0] and kept at 25 °C for 30 min. Approximately 100 μl from the previous step and homogenized in 900 μl of 1% acetic acid and centrifuged at 13,000 rpm for 5 min. Then 300 μl of the supernatant was separated into a new EP tube, and 700 μl of analysis buffer [0.35% (NH4)2MoO4, 2.34% H2SO4, and 1.4% VC] was added and incubated in a water bath at 42 °C for 30 min. Pi content was determined by measuring the absorbance of A820.
Calculation of water loss rate
WT and transgenic plants were grown under well-watered condition for 3 weeks. Healthy and uniform seedlings with intact rosette leaves were sampled from each genotype with sharp scissors. The rosette leaves were placed on dry filter papers in a light incubator with constant temperature and humidity and allowed to dehydrate naturally under room temperature. The leaves were weighed at 1, 2, 3, 4, 6, 8 and 10 h after separation, respectively, and the rate of water loss from the excised leaves at each sampling period was obtained by expressing the weights as a fraction of the fresh weight prior to dehydration and expressing it as a percentage.
Imaging and determination of chlorophyll fluorescence
Chlorophyll fluorescence was measured from rosette leaves of WT and ZmARF1 transgenic Arabidopsis under normal, drought or salt stress conditions. Rosette leaves were excised with sharp scissors and were immediately subjected to 20 min of dark acclimation. Initial fluorescence (F0), maximum fluorescence (Fm) and variable fluorescence (Fv) were determined with the FluorCam800MF system (Photon Systems Instruments, Ecotech). The experiment was conducted in triplicate, with three biological repeats each time. Chlorophyll fluorescence was calculated by the following formula.
Measurement of ion leakage
For membrane ion leakage measurement, five leaves disks (7 mm in diameter) of each treatment were picked from the fifth rosette leaf of the WT and transgenic seedlings under normal, drought and salt stress treatments. The leaf discs were collected in a 10 ml EP tube with deionized water for surface cleaning. Liquid was replaced with fresh 1 ml of deionized water and oscillated for 10 s, and the initial electrical conductivity (C1) was measured with an electro-conductivity meter. Samples were then placed in a vacuum pump for 30 min at a pressure of 0.8 to 1 MPa. After the vacuum treatment, the electrical conductivity was measured before (C2) and after (C3) boiling for 15 min with an electro-conductivity meter again. Total ion leakage (C) was determined by the following formula and expressed as a percentage.
RNA-seq analysis
Two-week old seedlings of WT and ZmARF1 transgenic Arabidopsis were transplanted in liquid medium with 1/2 MS (normal condition as control), low Pi, salinity (200 mM NaCl), and drought (200 mM d-mannitol) stress conditions, respectively. After 3 days treatment, seedlings were collected and prepared in three biological replicates for RNA-seq analysis. Total RNA extraction, library construction, transcriptome sequencing, transcriptome assembly, and functional annotation were performed by OE Biotech (Shanghai, China). High-quality reads were aligned against the Arabidopsis thaliana reference genome sequence (TAIR10 Genome Release) using HISAT2. Differentially expressed genes (DEGs) were identified using DEseq software with corrected p-value < 0.05. GO functional classification enrichment was performed using the online tools (http://geneontology.org/). GO terms with q < 0.05 were considered to be significantly enriched. The Integrative Genomics Viewer (IGV) (http://software.broadinstitute.org/software/igv) was used to visualize the reads of the selected genes.
Electrophoretic mobility shift assay (EMSA)
The fusion construct of full-length CDS of ZmARF1 fused with MBP and an empty MBP plasmid were used to transform Escherichia coli strain DE3 (TransGen, Beijing, China) and purified using amylose resin (NEB, E8021S#) as previously described (Wu et al. 2024). We synthesized 5ʹ biotin labeled probes of the auxin response element (AuxRE: TGTCTC) motif, together with unlabeled and mutated competitor (Sangon Biotech, Shanghai). EMSA was performed using the MBP-ZmARF1 recombinant protein, a biotin-labeled AuxRE probe, an unlabeled AuxRE, and its mutated competitor. EMSA was performed using a LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, Prod# 89880) following the manufacturer’s instructions, with slight modifications as previously described (Wu et al. 2024). Briefly, ZmARF1 protein and the biotin-labeled probe, with or without the unlabeled WT or mutant unlabeled probes, were incubated in 10 μl reaction volume at 25 °C for 30 min. The reaction mixtures were separated on 7% polyacrylamide gel and transferred to a nylon membrane. Signals were visualized using an imaging system (E-blot, Shanghai, China). All probes used are listed in Table S1.
Dual-luciferase assay
The fusion construct of full-length CDS of ZmARF1 fused with GFP in pGreenII62SK was used as effector. A ~ 2 kb promoter region of AtPOX was amplified and cloned into pGreenII0800-LUC to obtain ProAtPOX::LUC reporter construct. The reporter construct was co-expressed with effector either ZmARF1-GFP or pGreenII62SK-GFP as control in N. benthamiana leaves, as previously described (Hellens et al. 2005). The luciferase activity assay was performed using a dual luciferase reporter assay kit (Vazyme, Nanjing, China) according to the manufacturer’s protocol. Relative LUC activity was calculated as the ratio of LUC to Ren activity. Each sample was tested in triplicate. All primers used are listed in Table S1.
Results
Overexpression of ZmARF1 confers tolerance to low Pi stress via the regulation of root development
We had previously conducted a gene-based association analysis in 356 maize inbred lines and identified that ZmARF1 is associated with Pi stress tolerance in maize (Wu et al. 2024). To determine the potential role of ZmARF1 in low Pi stress response, we generated and selected three independent homozygous T3 ZmARF1-overexpressing lines with a high level of transgene expression (Fig. S1). For phenotypic detection, 7-day-old seedlings grown in 1/2 MS were transferred to either HP (high phosphate, 1/2 MS) or LP (low phosphate) conditions for 7 days (Fig. 1a, b). Ensuing results revealed that overexpression of ZmARF1 promotes root development (Fig. 1a, c–f), especially increasing root densities near the soil surface (Fig. 1a). Generally, low Pi stress reduced root development in all genotypes, but the ZmARF1 overexpressing transgenic plants recorded significantly (P < 0.05) increased root phenotypic traits, including root length, surface area, root volume and root tips compared to the WT (Fig. 1b–f). The results were in accordance with the theory that superior root morphology enhances phosphorus acquisition and promotes low Pi stress tolerance (Strock et al. 2018), suggesting that ZmARF1 confers low Pi stress tolerance by promoting root architecture reconstruction.
To further evaluate the responsiveness of ZmARF1 to low Pi stress, RT-qPCR and western blotting assays were performed to determine the transcript and protein abundance in ZmARF1-transgenic seedlings under HP and LP conditions, respectively. The results showed that transcript levels of ZmARF1 in the transgenic lines showed an undiscernible difference between the HP and LP conditions (Fig. 2a). On the contrary, protein abundance of ZmARF1 was significantly increased in the transgenic plants after LP treatment compared to HP (Fig. 2b). In addition to the initial comparison, a specific transgenic line (OE8#) with relatively lower ZmARF1 protein abundance was selected and used to carefully assess the accumulation dynamic of ZmARF1 protein following low Pi treatment. The results showed that ZmARF1 protein steadily accumulated after low Pi treatment and continued to increase throughout the entire day of treatment (Fig. 2c). This pattern of continuous increase in protein levels suggests that ZmARF1 protein abundance is directly induced by Pi starvation and that the response is time-dependent. The disparity between transcript levels and protein abundance of ZmARF1 due to low Pi treatment suggests that ZmARF1 might be exclusively regulated at the post-transcriptional level in response to low Pi stress. Moreover, protein abundance of ZmARF1 stabilized with MG132 treatment compared to the mock treatment (Fig. 2d), suggesting that ZmARF1 was regulated by the 26S proteasome mechanism and that addition of the proteasome inhibitor MG132 protected it from ubiquitination. This further indicates that ZmARF1 could potentially be exposed to some level of post-translational regulation.
ZmARF1 positively modulates Pi homeostasis by regulating the expression of PSI genes
Plants require sufficient phosphorus for optimal growth and development. Phenotypes analysis revealed that the transgenic plants adapted better to low Pi stress compared to the WT (Fig. 1). We hypothesized that the transgenic plants might be better at mobilizing, translocating, and utilizing Pi. Therefore, we quantified the Pi concentration in roots and leaves of the transgenic plants and WT. Interestingly, there was no difference in the leaf Pi concentration among all the genotypes, and the root Pi concentration was significantly (P < 0.05) higher in WT than in transgenic lines under HP condition (Fig. 3a). However, under LP condition, the transgenic lines had significantly higher Pi concentration compared to WT in both leaf and root tissues (Fig. 3b). Low Pi stress resulted in a dramatic decrease in Pi concentration, especially in leaves. The Pi concentration ratio under LP to HP in the root was significantly higher than in leaves, with 2.74, 1.96 and 1.25 folds increases in OE17#, OE8# and WT, respectively (Fig. 3c). Since root system architecture (RSA) is critical for Pi uptake, the lower Pi concentration in transgenic roots and the indistinguishable measure in leaves could herald a more efficient phosphorus transport than the wild type under HP. Under LP conditions, the transgenic lines could maintain higher phosphorus uptake and recycling efficiency compared to WT to sustain growth. This may also be the reason why the transgenic lines manifested favorable RSA-related traits (Fig. 1b). These results suggest that ZmARF1 plays a regulatory role in Pi homeostasis under low Pi stress, probably by influencing the mobilization and translocation of phosphates from source (roots) to sink (shoot).
To verify this hypothesis, we further investigated the expression of phosphorus starvation inducing (PSI) genes such as PHR1, PHT1;2, and PHO2 in different genotypes after high and low Pi treatments by RT-qPCR. Under HP conditions, the transcript abundance of the three target genes showed no noted differences between transgenic plants and WT (Fig. 3d–f). The expression levels of PHR1 and PHT1;2 even trended to decrease in two overexpressing lines (Fig. 3d, e). However, after LP stress treatment, all the detected PSI genes were dramatically upregulated, and the mRNA levels of PHR1, PHT1;2, and PHO2 in the transgenic plants were significantly higher than in WT (Fig. 3d–f). These results suggest that ZmARF1 modulates Pi homeostasis via the regulation of PSI genes expression, downregulating their transcription under sufficient phosphorus conditions, and upregulated when phosphorus is insufficient. The reduced expression of PSIs under HP conditions might be due to the superior RSA of transgenic plants, which increases the phosphorus absorption surface area. In contrast, the upregulated expression of PHR1, PHT1;2, and PHO2 under LP induction helps promote phosphorus absorption and transport from roots to shoots.
Overexpression of ZmARF1 confers tolerance to drought stress in transgenic Arabidopsis
Since ZmARF1 promotes root development under normal conditions, we hypothesized that ZmARF1 might be involved in other abiotic stress response processes, especially dehydration stress which is related to root function. Both drought and salinity initially result in osmotic stress, directly affecting the acquisition of water resources. To investigate the potential role of ZmARF1 in drought stress regulation, 3-week-old seedlings of the transgenic plants and WT were subjected to dehydration for 10 days. There was no discernible difference in the aboveground phenotype between the WT and transgenic plants prior to drought treatment (Fig. 4a). However, after drought treatment, the transgenic plants displayed strong tolerance to dehydration, while the WT showed susceptibility, as manifested by wilting of rosette leaves (Fig. 4a). Moreover, virtually all the WT plants could not recover after 3 days of rehydration, with survival rates below 15% (Fig. 4b). In contrast, the transgenic plants swiftly recovered after rehydration, with OE8# and OE17# recording more than 90% recovery rates, while OE22# recorded over 85% recovery rates (Fig. 4b). Analysis of the chlorophyll fluorescence, as indicated by Fv/Fm values showed no significant difference between the WT and transgenic plants under normal well-watered conditions (Fig. 4c). The Fv/Fm values of the WT plants decreased from ~ 0.8 under normal conditions to ~ 0.5 after drought treatment and rehydration. By contrast, Fv/Fm values of the transgenic lines remained greater than or equal to 0.79, which is indicative of healthy plants (Fig. 4c). The role of ZmARF1 in drought tolerance response was further examined by transferring the WT and transgenic plants to dry filter papers and allowing them to dehydrate at room temperature (Fig. 4d). After 10 h of dehydration, the rosette leaves of the WT plants were almost completely wilted, while the transgenic plants manifested a more stretched leaf morphology. After 12 h rehydration, the leaves of the transgenic lines showed be little or no wilting, while the damage to the WT plants appeared to be irreversible (Fig. 4d). Consistent with the drought stress response phenotype, the water loss and ion leakage rates of the WT plants were significantly higher than those of the transgenic plants (Fig. 4e, f). This suggests that overexpression of ZmARF1 confers tolerance to drought stress in transgenic Arabidopsis.
ZmARF1 is a positive regulator of salt stress response in Arabidopsis
To elucidate the function of ZmARF1 in regulating salt stress tolerance, 5-week-old WT and ZmARF1 overexpressing plants were irrigated with either normal water (Fig. 5a) or 200 mM NaCl solution (Fig. 5b) for two weeks and evaluated for salt stress tolerance phenotypes. There were no discernible differences in the phenotypic and physiological indices between the ZmARF1 transgenic plants and WT under normal growth condition (Fig. 5). On the contrary, salt stress impaired multiple aspects of plant growth, resulting in significant reduction in plant height, leaf color, flowering time, and overall plant vitality (Fig. 5a–d). Despite this, the transgenic plants overexpressing ZmARF1 displayed stronger tolerance, with significantly increased plant height (Fig. 5c) and chlorophyll content (Fig. 5d) compared to WT under salt stress. Consistently, the chlorophyll fluorescence (Fv/Fm) of the WT plants was about 2.5 folds lower under salt stress treatment compared to the transgenic plants (Fig. 5f, g). Moreover, salt stress caused approximately a fold higher ion leakage in the WT plants compared to the transgenic lines (Fig. 5e). Taken together, overexpression of ZmARF1 contribute to salt stress tolerance by reducing ionic stress and preventing chlorophyll degradation.
Transcription profiling revealed that POX is a downstream target of ZmARF1
To investigate the mechanism governing the role of ZmARF1 in regulating various abiotic stresses, transgenic plants overexpressing ZmARF1 were subjected to multiple stresses: low Pi, salt (200 mM NaCl) and osmotic (200 mM d-Mannitol) stresses. RNA-seq analysis showed that different stress treatments induced specific transcriptomic response profiles. There were 674/210, 1540/513, and 4067/3364 genes identified as specifically upregulated/downregulated by osmotic stress, low Pi stress, and salt stress, respectively (Fig. 6a). Each treatment had significantly more upregulated genes than downregulated genes (Figs. 6a and S2a–f), suggesting a positive role of ZmARF1 in enhancing stress tolerance in Arabidopsis. Notably, the number of gene expression changes induced by salt stress was significantly higher compared to the changes caused by low Pi and osmotic stress (Fig. 6a, b). This suggests that ZmARF1 plays a more dominant role in the response to salt stress compared to its involvement in low Pi and osmotic stress responses. Gene ontology enrichment analysis revealed an array of signaling-mediated biological processes under the respective stress treatments: response to oxidative stress, response to salt stress, response to osmotic stress under salt stress, root morphogenesis, response to osmotic stress and root development under low Pi stress, and root morphogenesis, response to oxidative stress and response to water deprivation under osmotic stress (Fig. S3).
Moreover, we identified 18/3 commonly upregulated /downregulated genes among the three abiotic stresses (Figs. 6b, c and S2g–j). Among these commonly upregulated DEGs, a peroxidase gene POX (AT4G30170) showed significant induction under all three stress conditions, with the highest relative expression level observed under salt stress compared to low Pi and d-Mannitol (salt > low Pi > d-Mannitol > normal) (Fig. 6d). Additionally, the IGV visualizated the differential transcript levels using the RNA-seq data (Fig. 6e), and the upregulated expression of POX under salt, low Pi and osmotic stress was corroborated by an RT-qPCR analysis (Fig. 6f). The consistent trend of induced expression of POX under the tested abiotic stresses suggests that it may be a potential downstream target of ZmARF1 and function as an intersection in the ZmARF1s regulated pathway for multiple abiotic stress response.
ZmARF1 positively regulates POX by directly binding to AuxRE in its promoter
To further investigate how ZmARF1 regulates the expression of POX, we carried out a transient expression assay using Nicotiana benthamiana leaves. Co-expression of ZmARF1-GFP with Pro-POX-LUC recorded the highest luciferase activity compared to co-expression of GFP with Pro-POX-LUC or Pro-POX-LUC alone (Fig. 7a–c). Members of the ARF family protein have been reported to positively regulate abiotic stress responses in plants by binding to the auxin response element (AuxRE: TGTCTC) in the promoters of stress-responsive genes. Analysis of the 2 kb promoter region of POX identified a putative AuxRE motif, suggesting that ZmARF1 could directly bind to its downstream target POX (AT4G30170) to form a cascade in the stress response pathway. We investigated this possibility through an EMSA. The EMSA was conducted using an MBP-ZmARF1 recombinant protein and a biotin-labeled AuxRE: TGTCTC motif. The results showed that recombinant MBP-ZmARF1 could bind to the biotin-labeled AuxRE probe, which was revealed by the presence of a supershifted band when recombinant MBP-ZmARF1 was incubated with the labeled probe (Fig. 7d). Moreover, the unlabeled competitor probes effectively interfered and reduced the binding ability of ZmARF1 in a dose-dependent manner, while the mutant competitor did not significantly interfere with the binding ability of ZmARF1 (Fig. 7d). These results confirmed that ZmARF1 physically binds to AuxRE in the promoter of POX (AT4G30170) and activates its transcription. To detect whether the POX’s transcriptional activity affected by abiotic stresses is mediated by ZmARF1, we redetected the luciferase activity after co-injection of ZmARF1-GFP with Pro-POX-LUC, followed by the addition of 200 mM NaCl, 200 mM mannitol, and H2O. Fluorescence imaging and subsequent LUC/REN ratio estimation revealed that osmotic stress such as salt and mannitol enhanced the transcriptional activation ability of ZmARF1 on the target POX. Thus, we identified that ZmARF1 acts as a key positive regulatory factor in multiple abiotic stresses and clarified its potential molecular mechanisms in activating the stress response factors in the reactive oxygen species metabolism pathway.
Discussion
The inception of the green revolution birthed the development of many crop varieties with improved stress tolerance and enhanced yields (Bailey-Serres et al. 2019). Due to global warming and climate change, the limitations imposed by abiotic stresses on crop productivity have been more menacing (Mora et al. 2015; Sanchez-Bermudez et al. 2022). Plants display tailored physiological and molecular responses when exposed to multiple stresses (Bai et al. 2018; Mota et al. 2021). In this study, we characterized ZmARF1 and its biological function in response to multiple abiotic stresses. It has been reported that ARF transcription factors are involved in the abiotic stress response (Bouzroud et al. 2020; Du et al. 2013). In this study, athough the transcript level of ZmARF1 in the transgenic plants did not show any discernable difference between high Pi and low Pi treatment (Fig. 1a), low Pi treatment increased the protein abundance of ZmARF1 over time (Fig. 1b, c). This suggests that ZmARF1 is an early Pi stress responsive factor whose responsiveness to low Pi stress is regulated at the protein level, and that the stability of its protein is highly regulated by low Pi stress. A sharp disparity between mRNA and protein accumulation of WRKY46 in response to multiple abiotic stress has been reported (Chi et al. 2013). Protein blot assays showed that 26S proteasome inhibitor MG132 stabilized ZmARF1 and mitigated its ubiquitination under Pi-sufficient condition (Fig. 1d). This suggests that ZmARF1 protein accumulation is not exclusively regulated by Pi stress imposition but also by 26S proteasome -mediated post-translational regulation.
Furthermore, transgenic plants overexpressing ZmARF1 displayed significant increase in root phenotypic traits (root length, root surface area, root volume, and root tip number) compared to the WT under low Pi stress (Fig. 2). This is consistent with a previous study in which ZmARF1 was reported to confer low Pi stress tolerance in maize by promoting root development under low Pi stress (Wu et al. 2024). Overexpression of ZmARF1 increased Pi accumulation in transgenic plants compared to WT under low Pi stress (Fig. 3a–c), suggesting that ZmARF1 may help maintain Pi homeostasis under low Pi stress. We hypothesized that this homeostasis is governed by ZmARF1’s role in regulating the expression of some low Pi stress inducible genes. The results showed that transcriptional levels of PHR1, PHT1 and PHO2 increased significantly under low Pi stress in transgenic lines (Fig. 3d–f). The role of high affinity Pi transporters in maintaining Pi homeostasis under low Pi stress is well documented (Chen et al. 2009; Guo et al. 2015; Liu et al. 2018). Members of the ARF family have been reported to regulate the expression of some high-affinity Pi transporters under low Pi stress (Huang et al. 2018; Wang et al. 2014).
The ability of plants to maintain water balance is crucial for overcoming drought and salt stresses. In this study, overexpression of ZmARF1 resulted in better growth and higher survival rates of transgenic plants under drought and salt stress treatments compared to WT (Figs. 4 and 5), indicating a positive response of ZmARF1 to these stresses. In terms of physiological traits, the ZmARF1-transgenic seedlings had the least excised leaf water loss and ion leakage after drought stress treatment compared to the WT (Fig. 4e, f). Similarly, the transgenic plants recorded the least ion leakage after salt stress treatment compared to the WT (Fig. 5e). Water loss and ion leakage are important physiological indices related to osmotic stress imposed by drought and salinity and can be used as accurate metrics for assessing plant resistance to abiotic stress (Lu et al. 2019; Mehari et al. 2022). One of the most prominent phenotypic manifestations of salt stress is abnormal development and inhibition of vegetative growth (van Zelm et al. 2020). Although salt stress generally affected vegetative growth of all the genotypes, the transgenic seedlings displayed superior vegetative growth compared to the WT, which was manifested by relatively higher plant heights (Fig. 5c). Chlorophyll fluorescence, mostly quantified as Fv/Fm, is a true indicator of photosynthetic capability of plants under adverse environmental stresses (Baker 2008; Huang et al. 2021). The results of this study revealed that the ZmARF1-transgenic Arabidopsis had higher chlorophyll content and Fv/Fm values than the WT under drought (Fig. 4c) and salt stress (Fig. 5d, g) respectively. The decrease in chlorophyll content in the WT compared to the ZmARF1-transgenic lines under drought and salinity stress is likely due to the ability of ZmARF1 overexpression to mitigate pigment photo-oxidation and chlorophyll degradation caused by oxidative stress imposed by drought and salinity treatments (Ma et al. 2020). Drought and salinity stresses have been shown to trigger a reduction in leaf chlorophyll content (Taïbi et al. 2016).
The molecular mechanisms by which plants respond to abiotic stresses encompass the activation of signal pathways that mediate biochemical and physiological responses (Su et al. 2020). The class II peroxidases have been reported to respond to multiple abiotic stresses. To unravel the molecular mechanism underlying the role of ZmARF1 in multiple abiotic stress responses, ZmARF1 transgenic Arabidopsis and WT seedlings under low Pi, drought and salt stress treatment or normal conditions were subjected to RNA-seq analysis. Transcriptomic profiling identified a member of the peroxidase superfamily gene, POX, whose expression was upregulated by the three abiotic stresses (Fig. 6), suggesting its possible role in the response of transgenic Arabidopsis to low Pi, drought, and salt stresses. Members of the peroxidase family genes are upregulated under oxidative stress conditions (Jin et al. 2019; Kim et al. 2008; Su et al. 2023). When plants are exposed to prolonged oxidative stress, it triggers the induction of many ROS such as O2−, H2O2 and OH−, which can cause cellular injuries through protein oxidation, lipid peroxidation, and DNA damage that may eventually result in cellular death (Hasanuzzaman et al. 2019, 2020). Members of the class III peroxidases catalyze the reduction of H2O2 in the peroxidative cycle by transferring electrons from various donors (Dat et al. 2000; Gill & Tuteja 2010). Our results showed that POX was a downstream target of ZmARF1, and that ZmARF1 bound to the AuxRE in the promoter of POX and upregulated its transcription (Fig. 7). We belief that ARF-POX module contributes to the plants’ ability to manage and mitigate oxidative stress under these adverse conditions.
Conclusion
This study characterized ZmARF1, a member of the ARF protein family predominantly localized to the nucleus. ZmARF1 acted as an important regulator of phosphate starvation responses, such that overexpression of ZmARF1 resulted in enhanced tolerance to low Pi stress, with marked increased in root length, surface area, volume and root tip number, and increased expression of low Pi stress responsive genes in transgenic Arabidopsis compared to WT. Additionally, our results showed that ZmARF1 conferred tolerance to drought and salt stress, with transgenic plants exhibiting increased chlorophyll parameters, reduced water loss and ion leakage under drought and salt stress compared to WT. Transcriptome profiling suggested that AtPOX was multi-stress responsive gene whose expression was induced by low Pi, drought and salt stresses in the transgenic plants. EMSA and dual-luciferase assays suggested that AtPOX was a downstream gene of ZmARF1 and that its transcription was upregulated by ZmARF1, contributing to anti-oxidative stress tolerance in the transgenic plants and conferring tolerance to low Pi, drought and salt stress. The results of this study suggest that ZmARF1 is a pleiotropic gene conferring tolerance to multiple abiotic stresses in transgenic Arabidopsis.
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Funding
This study was supported by Innovative Research Group Project of the National Natural Science Foundation of China (grant numbers 32030078, 32101785 and U21A20209), General Project of Natural Science Foundation of Sichuan Province (2022NSFSC0147 and 2023NSFSC0221), the key Research Program of the Department of Science and Technology of Sichuan province, China (2021YFN0034), and The Foundation of Major Science and Technology Application Demonstration Project of Chengdu, China (2022-YF09-00054-SN).
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F.W. and Y.L. conceived the research; L.L. and Y.G. participated in its design and coordination; Y.G., B.S.Y. Y.C., and D.S. performed the experiments; L.L., F.L., J.G., and Z.Z. collected and analyzed the data; L.L. and B.S.Y. drafted the manuscript; L.L., F.W., and Y.L. revised the manuscript. L.L., Y.G. and B.S.Y. contributed equally to this study. F.W. advised on all the experiments and revised the manuscript. All the authors have read and approved the final version of this manuscript.
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Liu, L., Gong, Y., Yahaya, B.S. et al. Maize auxin response factor ZmARF1 confers multiple abiotic stresses resistances in transgenic Arabidopsis. Plant Mol Biol 114, 75 (2024). https://doi.org/10.1007/s11103-024-01470-9
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DOI: https://doi.org/10.1007/s11103-024-01470-9