Abstract
Physiological responses from sensitive (S156) and resistant (R123) genotypes of ozone bioindicator, snap bean, were investigated after exposing the plants to cumulative, phytotoxic ozone amounts. Daily course of gas-exchange parameters showed delayed stomatal response in S156 leaves to environmental changes comparing to the response of R123 leaves. Potential photosynthetic quantum conversion, Stern-Volmer nonphotochemical quenching (NPQ), and maximum photochemical efficiency of PSII (Fv/Fm) values changed differently in the two genotypes between the first and last measuring days. We concluded that the higher ozone sensitivity originated at least partly from inferior regenerating and/or antioxidant capacity. Experimental protocol proved to be determinant on chlorophyll fluorescence parameters: Fv/Fm and NPQ declined at midday, and only the sensitive leaves showed a slight increase in NPQ between 12 h and 16 h. We explained these results by moderately high temperatures and shade-adapted state of our experimental plants under substantial ozone stress. On the base of temperature dependence of minimal fluorescence yield (F0), critical temperature proved to be higher than 32.7°C for Phaseolus vulgaris under these conditions. We found a strong linear correlation between NPQ and nonphotochemical quenching of F0, indicating that NPQ was determined mostly by energy-dependent quenching (qE). The qE is the light-harvesting complex located component of NPQ and depends on the amount of zeaxanthin molecules bound in PSII proteins. Thus, difference between daily courses of NPQ in the two genotypes was probably due to different ways of utilization of the zeaxanthin pool under the interactive effect of ozone and moderate heat stress.
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Abbreviations
- ABA:
-
abscisic acid
- AOT40:
-
accumulated O3 exposure over a threshold of 40 ppb
- C i :
-
intercellular CO2 concentration
- E :
-
transpiration
- F0 :
-
minimal fluorescence yield of the dark-adapted state
- Fv/Fm :
-
maximum photochemical efficiency of PSII
- g s :
-
stomatal conductance
- LHCIIb:
-
the major chlorophyll-protein complex of LHC associated with PSII
- NPQ:
-
Stern-Volmer nonphotochemical quenching
- P N :
-
net photosynthetic rate
- QA :
-
primary quinone acceptor of PSII
- qE :
-
energy-dependent component of NPQ
- qF0 :
-
nonphotochemical quenching of F0
- qNP :
-
nonphotochemical quenching coefficient
- qP :
-
photochemical quenching coefficient
- R123:
-
ozone-resistant bean genotype
- RFd:
-
potential photosynthetic quantum conversion
- ROS:
-
reactive oxygen species
- S156:
-
ozone-sensitive bean genotype
- Tc :
-
critical temperature
- Tl :
-
leaf temperature
- VPD:
-
vapour pressure deficit
- ΦPSII :
-
effective quantum yield of PSII
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Acknowledgements: This research has been supported by the Hungarian-Slovenian Intergovernmental Science and Technology Cooperation Programme (SI-2/08). This paper could not be written without the support of the Research Station of Cegléd of Fruit Research Institute, National Agricultural Research and Innovation Centre. Authors also thank the referees for providing constructive comments and suggestions. We would like to acknowledge Zoltán Nagy for revising the final version of this paper.
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Villányi, V., Ürmös, Z., Turk, B. et al. Photosynthesis of ozone-sensitive and -resistant Phaseolus vulgaris genotypes under ambient ozone and moderate heat stress. Photosynthetica 52, 604–613 (2014). https://doi.org/10.1007/s11099-014-0070-5
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DOI: https://doi.org/10.1007/s11099-014-0070-5