High levels of acid phosphatase activity in nerve cells allow methods for its detection to be used in morphofunctional studies of nervous tissue elements. Using neurons in the stellate ganglion in cows as an example, the bodies and processes of nerve cells are shown to have different levels of enzyme activity. Nerve endings have consistently high levels. This method provides for detailed morphological investigation of neurons and assessment of their interactions. This report provides a detailed description of an improved method for identifying acid phosphatase activity in nervous tissue structures.
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References
G. G. Aminova, “Activity of phosphatases in sympathetic and spinal ganglia in cattle,” Trudy Mosk. Veter. Akad., 22, No. 2, 21–29 (1958).
M. Berston, Enzyme Histochemistry [Russian translation], Mir, Moscow (1965).
T. V. Davydova, “Comparative morphology and histochemistry of the midbrain in various ruminants,” Trudy Mosk. Veter. Akad., 22, No. 1, 49–56 (1958).
M. Dixon and E. Webb, Enzymes [Russian translation], Mir, Moscow (1982), Vol. 1.
G. Sh. Zhanchipov, Acid Phosphatase Activity and the ‘Orthophosphate Concentration’ in De- and Regenerating Nerves and Nerve Fibers: Auth. Abstr. Master’s Thesis in Biol. Sci., Moscow (1957).
N. N. Petropavlovskaya, “Reactions of nerve cells in the gangliose and cranial cervical ganglia to damage to their processes,” Trudy Mosk. Veter. Akad., 22, No. 1, 23–26 (1958).
A. N. Trukhachev, Morphofunctional State of the Sensorimotor Cortex in Nonuniform Electromagnetic Irradiation: Auth. Abstr. Master’s Thesis in Med. Sci., Yaroslavl (2009).
I. L. Tsvetkov, S. L. Zarubin, G. A. Urvantseva, et al., “Acid phosphatase in hydrobionts as an indicator enzyme for biochemical adaptation to the actions of toxic substances,” Izv. Akad. Nauk. Ser. Biol, No. 5, 539–545 (1997).
A. M. Chilingaryan, Some Data on the Histochemical Cytoarchitectonics of Nerve Plexuses in the Intestine: Auth. Abstr. Master’s Thesis in Biol. Sci., Moscow (1954).
E. I. Chumasov, E. S. Petrova, and D. E. Korzhevskii, “Innervation of the heart in rats (an immunohistochemical study),” Morfologiya, 135, No. 2, 33–37 (2009).
M. E. El’bert, Some Data on the Histochemistry of Acid Phosphatase in Intestinal Neurons. Morphology and Cytoarchitectonics Auerbach’s Plexus in Cats and Dogs: Auth. Abstr. Master’s Thesis in Biol. Sci., Moscow (1956).
D. A. Bodian and R. C. Mellors, “The regenerative cycle of motoneurons with special reference to phosphatase activity,” J. Exp. Med., 81, No. 5, 469–487 (1945).
G. Gomori, “Distribution of acid phosphatase in the tissues under normal and pathologic conditions,” Arch. Pathol., 32, 189–199 (1941).
A. Wolf, E. A. Kabat, and W. Newman, “Histochemical studies on tissues enzymes. III. A study of the distribution of acid phosphatases with special reference to the nervous system,” Am. J. Pathol., 19, 423–439 (1943).
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Translated from Morfologiya, Vol. 150, No. 6, pp. 66–68, November–December, 2016.
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Aminova, G.G. Determination of Acid Phosphatase Activity in Nervous Tissue. Neurosci Behav Physi 47, 1015–1018 (2017). https://doi.org/10.1007/s11055-017-0505-0
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DOI: https://doi.org/10.1007/s11055-017-0505-0