Abstract
To reveal responses of photosynthetic physiological characteristics in Phoebe bournei seedlings to different light qualities and provide a basis for light environment regulation in seedling cultivation and under-canopy regeneration, we analyzed the photosynthetic physiological characteristics of P. bournei seedlings under five types of light qualities (white, red, blue, green, red: blue = 1:1) with the same photosynthetic photon flux density of 100 ± 5 μmol·m−2·s−1. The results showed that blue light significantly promoted chlorophyll content, Rubisco, RCA and photosynthetic rate (Pn), but water use efficiency (WUE) was reduced by blue light. The red light treatment exhibited the highest initial fluorescence (Fo), but wasn't conducive to the accumulation of photosynthetic pigments and had the lowest maximum photochemical efficiency (Fv/Fm) and potential photochemical efficiency (Fv/Fo). The seedlings treated by green light showed the lowest Pn, Tr, WUE, rubisco, with the highest Fv/Fm and Fv/Fo. Red-blue light significantly increased WUE, photosynthetic electron transfer rate (ETR), actual photosynthetic efficiency Y(II), and quantum yield of regulated energy dissipation Y(NPQ) in PSII of P. bournei seedlings, with the second highest Pn across treatments. In conclusion, the effects of single light quality on photosynthetic characteristics of P. bournei seedlings exhibited both advantages and disadvantages. The combination of red and blue light, which integrated the advantages of single light quality, enhanced the photosynthetic performance and photoprotective ability of P. bournei seedlings. It promoted the energy conversion and utilization efficiency of PSII, resulting in the best carbon assimilation efficiency. Therefore, red-blue light promoted the growth and development of Phoebe bournei seedlings.
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Introduction
Light is the source of energy for plant photosynthesis, and the photomorphogenesis of plants is controlled by light quality (Fang et al. 2021). Plant photoreceptors and photosynthetic pigments jointly receive light signals, which regulate both stomatal movement and chloroplast structure, as well as photosynthetic pigments (Li et al. 2013). They can activate or inhibit a series of physiological responses in plants and control plant photomorphogenesis (Li et al. 2013). Various light qualities or wavelengths exert distinct biological effects on morphological structure, chemical composition, photosynthesis, organ growth, and plant development.
Many studies on the effect of light quality on plant photosynthetic characteristics have shown that controlling spectral composition can optimize plant quality and yield (Paradiso and Proietti 2022). It has been reported that red light (R) can regulate the development of photoreceptors and photosynthetic organs in some species, activate the photosynthetic process and regulate morphogenesis, thereby increasing photosynthetic products and promoting plant growth (Sb et al. 1995; Yu et al. 2017). However, some studies showed that red light itself was not suitable for cultivating seedlings. For instance, Lactuca sativa, Boehmeria nivea, and Fragaria × ananassa grown under red light exhibited lower photosynthetic capacity compared to tose cultivated under blue light (Johkan et al. 2012; Rehman et al. 2020; Yoshida et al. 2016). These results indicated that there are differences in the response of different plants to light quality. Light quality plays an important role in regulating the biosynthesis of photosynthesis-related pigments (Zhang et al. 2021). Blue light is very important for chloroplast development and photosynthesis (Hogewoning et al. 2010; Sb et al. 1995). The chlorophyll content, carotenoid content, and chlorophyll a/b ratio of increased under blue light, which increased stomatal conductance and intercellular CO2 concentration through inducing stomatal opening (Hogewoning et al. 2010; Sb et al. 1995). High-intensity short-wave green (G) light can effectively activate the photosynthetic rate of Lactuca sativa leaves and significantly increase plant growth and net photosynthetic rate (Johkan et al. 2012). Green light plays a special role in regulating plant growth and metabolism, which can penetrate deeper foliage and understory plants, promoting plant growth by stimulating the carbon assimilation of plants below the plant canopy (Kim et al. 2004). Wang et al. (2015) showed that a single red or blue light could not meet the growth requirements of plants. A high ratio of red-blue light was beneficial to the growth of plants at the seedling stage (Wang et al. 2015). Blue light (425–490 nm) and red light (610–700 nm) have been identified as the optimal spectra for plant photosynthesis (Chang and Chang 2014). Red-blue light can increase the accumulation of nitrogen in leaves, which improves photosynthetic rate, light energy utilization and product quality by increasing leaf area and biomass, and promotes plant growth and development (Han et al. 2015; Ohashi-Kaneko et al. 2006). At present, there are few reports on the response mechanisms of woody seedlings to light quality, and more studies on crops (Riikonen 2021). Previous studies have shown that light quality can affect the nutrient content of both aboveground and underground parts of Pinus koraiensis seedlings (Wei et al. 2020). Red light promotes the growth of Picea abies seedlings (OuYang et al. 2021). Song et al. (2020) explored the effect of light quality on Camellia oleifera at the physiological and transcriptomic levels, and found that plants under blue light showed better growth. The importance of light quality for plant growth and its role as a pivotal signal in plant development are evident (Fu and Chin 1992; Zheng 1983).
Phoebe bournei (Hemsl.) Yang belongs to the family of Lauraceae, which is a precious tree species, and a shade tolerant species (Fu and Chin 1992; Zheng 1983). Because of its excellent timber and beautiful wood grain, it became a high-grade building material for the imperial palace in ancient China (Tang et al. 2020). P. bournei is naturally distributed in the subtropical climate zone with a warm and humid conditions (An et al. 2022). The wild resources of P. bournei have significantly depleted due to prolonged logging, exploitation, and utilization. In recent years, P. bournei plantation forestry has gradually increased, and the demand for seedlings has increased. In the investigation of P. bournei natural forests in the field, it was observed that there were significant differences in the number and growth of P. bournei seedlings in different understory light environments (Han et al. 2021), which might be caused by the heterogeneity of light intensity and light quality. Some studies with appropriate shade treatment increased the photorespiration of P. bournei, which can improve photosynthetic CO2 assimilation by decreasing photorespiratory metabolites (Tang et al. 2020). The biomass, chlorophyll content and net photosynthetic rate of P. bournei increased significantly with increasing degree of shading (An et al. 2022). The rapid growth of P. bournei seedlings may be the result of accelerated expression of genes related to photosynthesis and chlorophyll, which enable plants to maintain high photosynthetic rates even under low light conditions (An et al. 2022). However, the effect of light quality on P. bournei seedlings was neglected. The spectral component received by P. bournei seedlings under the canopy will change due to the absorption, reflection and scattering of incident light by the upper layer of the forest canopy. While, it is unclear about how the light quality affects the photosynthetic physiological characteristics and growth of P. bournei seedlings. Therefore, we designed a pot experiment with different types of light qualities to investigate the effects of light quality on photosynthetic pigments, gas exchange parameters, key photosynthetic enzyme activities and chlorophyll fluorescence characteristics of P. bournei seedlings. The objective was to reveal the response and adaptation of P. bournei seedlings to different light quality. Our research aims to provide a scientific basis for regulating seedling growth through artificial light supplementation.
Materials and methods
Experimental material and light treatments
P. bournei seedlings were grown in germination trays (seeds were collected from the same tree in Baili Village, Gangdu Town, Huishui County, Guizhou Province, China). Seedlings with similar growth (7.0 ± 0.85 cm in height; 1.20 ± 0.08 mm in diameter) were selected and transplanted into pots filled with a peat: perlite = 4:1 mixed matrix in May. Two seedlings were planted per pot, with each pot measuring 15.5 cm in diameter and 14 cm in height. After the seedlings have recovered, placed them in a light-light quality incubator (FH-740, Hipoint, Taiwan) with different light quality for culture experiments.
The light quality and light intensity were measured under P. bournei natural forest in Taijiang County, Guizhou Province, China, and the parameters of the photosynthetic photon flux density in incubator were set according to the data from the field environment. All light sources were provided by light-emitting diodes (LEDs). Four kinds of light quality treatments were set, namely red light (R, 660 nm), blue light (B, 450 nm), green light (G, 525 nm) and red-blue light BR (red light: blue light = 1:1). White light was used as a control (CK). Each treatment consisted of three replicates, with 12 seedlings per replicate. The parameters of the incubator were set as follows: photon flux density of 100 ± 5 μmol·m−2·s−1, relative humidity of 70% ± 5%, photoperiod of 14 h/10 h and temperatures of 28 °C ± 0.5 °C/20 °C ± 0.5 °C (day/night). The substrate was kept moist by watering it once every three days. Treatment was started on June 25, 2020 and lasted for 60d. At the end of the treatment, the fully expanded leaves with vigorous growth on the upper portion of the seedlings were selected for determination of the indices.
Measurement of chlorophyll content
Ethanol extraction method was used for determination of chlorophyll (Min et al. 2018). Fresh leaves of P. bournei, were picked, washed and dried, while the main veins were removed. 0.1 g of functional leaves were weighed and put into a scaled test tube and added into 10 ml 95% ethanol to extract for 48 h under dark conditions. After the leaves turned white, the absorbance of chlorophyll was measured by UV spectrophotometer at 665 nm, 649 nm and 470 nm. According to the specific absorption coefficient of chlorophyll in 95% ethanol solution, the chlorophyll content was calculated by following formula (Gao 2006).
where V (ml) is the total volume of ethanol extract, and W (g) is the weight of fresh leaves.
Measurement of gas exchange parameters
In mid-September 2020, the gas exchange parameters of P. bournei seedlings were determined from 9 to 10 a.m. on a sunny day by portable open infrared gas exchange analyzer system (LI-6800; LI-Cor Inc., Lincoln, NE, USA) and a red and blue light source leaf chamber (6800–02). Firstly, the leaves of P. bournei seedlings were induced for 30 min in the leaf chamber with the optical quantum flux density of 1000 μmol·m−2·s−1, CO2 concentration of 400 μmol·mol−1 and temperature of 28℃. The parameters including net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr) were obtained, and the leaf water use efficiency of signal leaf was calculated as: (WUE) = Pn /Tr.
Measurement of chlorophyll fluorescence parameters
The chlorophyll fluorescence parameters were determined using a portable multichannel continuous fluorescence monitor (Monitoring-PAM, Heinz Walz GmbH, Effeltrich, Germany). The leaves were determined after 1 h of dark adaptation. The measured indices include initial fluorescence (Fo) and maximum fluorescence (Fm). The variable fluorescence Fv (Fv = Fm-Fo), maximum photochemical efficiency of PSII (Fv/ Fm), and potential photochemical efficiency of PSII (Fv /Fo) were calculated based on Fo and Fm.
Meanwhile, the rapid light response curves of P. bournei leaves under different light quality treatments were measured by a fluorescence monitor, and the photosynthetically active radiation (PAR) gradients were set to 0, 90, 125, 190, 285, 420, 625, 820, and 1150 μmol·m−2·s−1. According to the method proposed by Ye et al. (2010), the initial slope (α), maximum electron transfer rate (Jmax) and saturated light intensity (PARsat) were fitted with an online model developed by Ye (http://photosynthetic.sinaapp.com/).
Measurement of photosynthetic key enzyme activities
The Rubisco and RCA contents of P. bournei leaves were measured by ELISA kits (Shanghai Keshun Biotechnology Co., LTD., Shanghai, China). The Rubisco and RCA contents of seedlings were determined by the double antibody sandwich assay method. First, the functional leaves of uniform size and at the same node position from each treatment were collected and rapidly frozen in liquid nitrogen, stored at − 80 °C. Second, leaf tissues (1 g) were ground into powder in liquid nitrogen and homogenized in 9 mL precooled enzyme extract buffer. The homogenate was centrifuged in a microcentrifuge at 8000 rpm at 4 °C for 30 min. The supernatant and Reagents 1 through 5 were then added in turn to a 96–well plate according to the instructions at room temperature (25 °C). Finally, absorbance (OD value) was measured at 450 nm using a microplate reader (Tecan Austria GmbH; Untersbergstr, 1A, A-5082 Grodig; Austria), and the enzyme content for each treatment was calculated based on the corresponding standard curve (Shuya et al. 2022; Li et al. 2020).
Microtiter plate wells were coated with purified Rubisco or RCA capture antibodies to prepare solid-phase antibodies. Then Rubisco or RCA was added to the coated micropores, and then combined with the HRP labeled detection antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB was added for color development. The TMB was converted into blue under the catalysis of HRP enzyme and into the final yellow under the action of acid. The shade of the color is positively correlated with the Rubisco or RCA in the plant.
Statistical analysis
Experiments were performed in a comprehensively random design. Values presented are mean ± standard deviation (SD) of three replicates. Microsoft Excel (2010) was used to calculate the basic test data. All data were tested for normality and homogeneity of variance (Levene’s test) before statistical analyses, and when necessary, were log-transformed. The data were analyzed using one-way analysis of variance (ANOVA), and mean values were compared using the Duncan’s multiple range test (p < 0.05). Pearson correlation analysis was used to determine the correlation between photosynthetic parameters (p < 0.05). All statistical analyses were conducted using SPSS 26.0 for Windows. Origin 2021 software was used for chart drawing.
Results
Response of photosynthetic pigments to light quality in P. bournei seedlings
Different light quality treatments had different effects on photosynthetic pigments of P. bournei seedlings (Fig. 1), and the content of Chla and Chlb differed significantly between treatments (p < 0.05). Under blue light, the contents of Chla, Chlb, and Car in P. bournei seedling leaves reached their highest values, which were significantly higher than those under other light quality treatments (p < 0.05). Specifically, Chla, Chlb, and Car increased by 9.58%, 19.42%, and 12.77%, respectively, compared with the control (CK). However, the Chla/b was the lowest under the blue light, which was 8.52% lower than CK. The differences in content of Chla and Chlb between the green light and red-blue light treatments were not significant (p > 0.05). The contents of Chla and Chlb of P. bournei seedling leaves under red light treatment were lower, but its Chla/b was the highest, which was 1.15 times higher than that of CK g.
Response of photosynthetic characteristics to light quality in P. bournei seedlings
Gas exchange parameters
The results demonstrated that light quality had significant effects on gas exchange parameters of P. bournei seedlings (Table 1). The Pn, Tr and Gs of leaves in P. bournei seedlings under blue light were significantly higher than those under other light treatments (p < 0.05), which were 1.47, 1.60 and 1.85 times of CK, respectively. Its Ci was second only to the green light treatment, 10.26% higher than CK, and the WUE is only higher than that of the green light treatment. The Ci of red-blue light treatment was the lowest, and there was no significant difference compared with CK and red light treatment (p > 0.05). The WUE of red-blue light treatment was 76.95% higher than that of green light treatment. The Pn, Tr and WUE of leaves under green light were lower than CK by 67.41%, 44.14% and 41.35%, respectively. However, its Ci was significantly higher than that of the other light quality treatments, and was 22.46% higher than CK. The stomatal conductance of P. bournei leaves under red light was the lowest, only 50.86% of CK.
Content of photosynthetic enzymes
The Rubisco enzyme content of P. bournei leaves under blue light was the highest, but there was no significant difference among CK, green light and red-blue light treatments (p > 0.05) (Fig. 2). The content of Rubisco enzyme in red light was the lowest, and the Rubisco enzyme content in blue light was increased by 22.58% compared with that in red light. RCA content of different treatments was significantly different (p < 0.05), and the enzyme content in descending order was B > CK > BR > R > G. RCA of blue light treatment was increased by 11.67%, 80.61%, 122.89% and 55.37% compared with CK, red light, green light and red-blue light, respectively.
Response of chlorophyll fluorescence parameters to light quality in P. bournei seedlings
Chlorophyll fluorescence parameters
There were significant differences in fluorescence parameters of P. bournei seedlings under different light qualities (p < 0.05) (Table 2). The initial chlorophyll fluorescence (Fo) was R > CK > G > BR > B. The Fo of red light treatment increased by 10.14% compared with CK, and increased by 55.09% compared with blue light treatment. The maximum fluorescence (Fm) showed G > CK > BR > B > R. The maximum photochemical efficiency (Fv/Fm) and potential photochemical efficiency (Fv/Fo) of PSII were the highest under green light and the lowest under red light. There was no significant difference in Fv/Fo and Fv/Fm between red-blue light and blue light treatments (p > 0.05). The Fm, Fv/Fm and Fv/Fo of P. bournei seedlings treated with green light increased by 80.57%, 16.79% and 137.45% compared with the lowest red light treatment, and increased by 3.2%, 2.21% and 8.35% compared with CK, respectively.
The increase in photosynthetically active radiation (PAR) led to a rapid decline in qP, followed by a gradual stabilization (Fig. 3). When PAR < 300 μmol·m−2·s−1, the qP of blue and red light treatments were higher. When PAR > 300 μmol·m−2·s−1, the qP of red-blue light treatment exceeded that of blue light treatment and was higher than that of other treatments. Under the same PAR condition, the qP of red light treatment decreased the fastest, and the qP of green light treatment was the lowest. This indicated that the PSII reaction center of P. bournei was more open and absorbed more light energy for photosynthesis under red-blue light. Non-photochemical quenching (NPQ) represents the dissipation of light energy absorbed by PSII that cannot be utilized for photosynthetic electron transport, manifested as heat dissipation. The trend in NPQ exhibited an inverse relationship with that of qP. With increasing PAR, NPQ initially increased and subsequently reached a plateau. When PAR < 400 μmol·m−2·s−1, NPQ increased almost linearly. When PAR was between 400 and 1100 μmol·m−2·s−1, the variation of NPQ gradual reached saturation, and the NPQ showed CK > BR > B > G > R. It exhibited that most of the light energy absorbed by leaves of CK could not be used for photosynthetic electron transfer, but dissipated in the form of heat. The NPQ of red and green light treatments were low, so the light protection ability was poor, which may be damaged by light.
The light response of chlorophyll fluorescence and energy partitioning
The simulation of the ETR-response curve revealed distinct trends among the various light quality treatments. With the increase of photosynthetically active radiation, the apparent electron transfer rate continued to increase, and finally tended to be stable and reached saturation (Fig. 4A). Within the range of 200–1200 μmol·m−2·s−1 of PAR, the order of ETRs was BR > B > CK > R > G under identical conditions. The α values of CK and green light treatment were lower than those of red light, blue light and red-blue light (Table 3). The PARsat values of CK and red-blue light treatment were higher, which was 75.58% and 70.63% higher than that of red light. The Jmax of red-blue light treatment was the highest, 21.30% higher than CK. Red-blue light significantly increased ETR and α rapidly. Under different light qualities, the energy distribution of PSII in the leaves of P.bournei seedlings changed with PAR (Fig. 4B-F). As the PAR increased, the Y(II) initially decreased and then reached a plateau, while the Y(NPQ) exhibited an initial increase followed by stabilization. The Y(NO) fluctuated in accordance with the values of Y(II) and Y(NPQ). In energy allocation, the proportion of Y(II) in CK was slightly lower than that in blue and red-blue light treatments, while Y(NPQ) was the highest. This indicated that the leaves accept excess light intensity under white light, but white light treatment can dissipate excess light energy into heat energy to protect themselves, and most of the energy is used for light protection. Compared with other treatments, Y(II) and Y(NPQ) were lower in green and red light treatments, while Y(NO) was higher. These results indicated that the regulation mechanism of photochemical energy conversion and protection (such as heat dissipation) of leaves under red and green light were insufficient to fully dissipate the light energy absorbed by seedlings. The seedlings were exposed to more light than they can receive and may be damaged by light. Y(II) was higher under blue and red-blue light treatments. However, compared with blue light treatment, red-blue light treatment had higher Y(NPQ) and lower Y(NO), so as to achieve the best photosynthetic efficiency.
Correlation analysis
The correlation analysis (Fig. 5) showed that Rubisco in the leaves of P. bournei seedlings was extremely significantly correlated with Chla and Chlb (p < 0.001), and was significantly correlated with Pn, Tr and RCA (p < 0.05). The RCA was significantly correlated with Pn, Tr, Chla and Chlb (p < 0.05). The Tr was significantly correlated with Pn (p < 0.01), and was significantly correlated with Chla and Chlb (p < 0.05). The Chla was significantly correlated with Chlb (p < 0.001). The Fv/Fm was significantly correlated with Fv/Fo (p < 0.001). A significant negative correlation was observed between Gs and Fo, as well as between Ci and WUE (p < 0.05). However, no significant correlation was found between Car and the other measured parameters.
Discussion
Relationship between light quality and photosynthetic pigments
Plants sense light signals through photoreceptors (Galvao and Fankhauser 2015). Among these photoreceptors, phytochromes are responsible for perceiving red light, while cryptochromes and certain phytochromes perceive blue light. Additionally, some phytochromes are also capable of perceiving green light (Galvao and Fankhauser 2015). Upon perceiving changes in the light quality of the environment, the photoreceptor initiates a cascade of cellular responses, governing its own metabolic processes, energy regulation, and exerting control over plant photomorphogenesis, photosynthetic pigments, enzyme synthesis (Taiz and Zeiger 2010). Previous studies have suggested that spectra including blue wavelengths enhance the production of photosynthetic pigments (Dieleman et al. 2019; hogewoning et al. 2010). Blue light is required for chlorophyll synthesis and chloroplast formation in higher plants (Taiz and Zeiger 2010). Blue light stimulates the production of chlorophyll and carotenoids through cryptochrome, which is conducive to the accumulation of pigments (Fan et al. 2013a, 2013b; Sood et al. 2005; Tanaka et al. 1998; Weller et al. 2001). It can reverse the inhibitory response induced by red light, while red light is not conducive to pigment accumulation (Fan et al. 2013a, 2013b; Sood et al. 2005; Tanaka et al. 1998; Weller et al. 2001). However, in the study of Boehmeria nivea and Lactuca sativa, it was found that the content of chlorophyll and carotenoids increased in red light treatment, suggesting that red light was more conducive to the synthesis of photosynthetic pigments than blue light (Rehman et al. 2020; Shimizu et al. 2011). In this study, the contents of chlorophyll components and carotenoids in the leaves of P. bournei seedlings under blue light treatment was the highest, while that under red light treatment was lower. The chlorophyll content of red-blue light treatment was significantly increased because blue light reversed the inhibitory response induced by red light. Chlorophyll a is a component of both photosynthetic reaction centers and the light-harvesting antennae, while chlorophyll b is synthesized from chlorophyll a and is only located in the antenna complexes (Fang et al. 2021). Chlorophyll b has a strong absorption around 450 nm, which is a region of light that chlorophyll a cannot effectively absorb. It has a spectral absorption region compensation effect and can capture a wider range of light (Tanaka and Tanaka 2011). Chlorophyll b is also required for the stabilization of the major of the structure of light-harvesting complex (LHC) proteins (Tanaka and Tanaka 2011). The peak wavelengths of chlorophyll a and b absorption spectra differ by about 20 nm (Kume et al. 2018; Xu et al. 2020). Chlorophyll a/b is an important determinant of the light absorption efficiency of photosynthesis (i.e. antenna size), which can reflect the changes of the optical system (Kume et al. 2018; Xu et al. 2020). The results of this study showed that the chlorophyll a/b value was lowest under blue light. Blue light may promote the conversion of chlorophyll a to chlorophyll b to a large extent, and the light energy absorption capacity is was improved. Then the chlorophyll a/b value was positively correlated with the red/blue ratio. This contradicts previous studies investigating the effects of light quality on Fragaria × ananassa, Lycopersicon esculentum and Dendrobium huoshanense. Their results showed that the chlorophyll a/b value increased under blue light (Johkan et al. 2010; XUKai et al. 2004; Yang et al. 2018). The observed disparity between our findings and previous results could be attributed to the divergent light requirements among plant species, with each species exhibiting specific responses to varying light qualities. Then, variations in photosynthetic pigment content may arise from the differential light perception capabilities of different plant photoreceptors.
Relationship between light quality and photosynthetic characteristics
Rubisco is an enzyme that catalyzes the assimilation of carbon dioxide in photosynthesis and is the most abundant leaf protein on earth (Carmo-Silva et al. 2015). As the core of plant productivity, it is the only enzyme that can support the net assimilation of carbon and increase biomass (Carmo-Silva et al. 2015). Rubisco activase (RCA), a catalytic chaperone of Rubisco, which can regulate the activity of Rubisco enzyme and is one of the key regulatory mechanisms of light on carbon fixation (Nagarajan and Gill 2018). Like other AAA + ATPases, RCA uses the energy from ATP hydrolysis to remodel the conformation of its target protein, Rubisco (Carmo-Silva and Salvucci 2013; Nagarajan and Gill 2018; Snider et al. 2008). Studies by Lorimer (2003) and Vicente et al. (2011) showed that the content of chlorophyll and Rubisco was often considered as indicators of the light capture and Calvin cycle ability of leaves. The rate of photosynthesis and biomass accumulation largely depended on the content and activity of Rubisco (Lorimer 2003; Vicente et al. 2011). In the study of Lycopersicon esculentum, Izzo et al. (2020) found that the Rubisco content and photochemical efficiency of seedlings under red light were the lowest, while the heat dissipation was the highest, and the photosynthetic capacity decreased. In addition, most studies showed that blue light significantly increased Gs and Rubisco activity, up-regulated the transcription levels of genes encoding Calvin cycle enzymes and Rubisco small subunits, and significantly improved the photosynthetic efficiency of genes encoding which mediated regulation of Rubisco enzyme activity (RCA) (Hung et al. 2022; Kuno and Furuya 2000; Li et al. 2021b; Su et al. 2014; Tepperman et al. 2001; Wang et al. 2009). The biosynthesis of Rubisco and the expression of RCA increased in seedlings grown under blue light (Hung et al. 2022; Kuno and Furuya 2000; Li et al. 2021b; Su et al. 2014; Tepperman et al. 2001; Wang et al. 2009). In this study, Rubisco and RCA in the leaves of P.bournei seedlings were significantly correlated with Chla, Chlb, Pn and Tr. The indicators exhibited the highest values under blue light, lower values under green and red light, and the red-blue light treatment showed values comparable to CK. These findings were consistent with previous studies on cabbage and cucumber (Hu et al. 2017; Su et al. 2014). Studies conducted by Hu et al. (2017) and Su et al. (2014) indicated that alterations in Pn were consistently accompanied by changes in Gs, Rubisco synthesis, as well as the transcription and expression of key genes involved in the Calvin cycle. Among them, blue light was conducive to photosynthesis of seedlings. The content of Rubisco was the highest in the seedlings induced by blue light, which was more important for the function of photosynthetic organs than red light. Therefore, we speculated that blue light mainly acted on the cryptochrome in the leaves of P.bournei seedlings, promoted the synthesis of more photosynthetic pigments, and significantly increased the content of Rubisco enzyme and RCA protein. Then it enhanced the ability of Calvin cycle, catalytic carbon dioxide assimilation. Therefore, blue light increased the Pn and Tr of leaves, and the photosynthetic performance of P. bournei seedlings was improved.
Blue light is an environmental signal mediated by its specific photoreceptors that induces stomatal and chloroplast movement and development (Terashima et al. 2016; Inoue and Kinoshita 2017). Higher stomatal conductance can promote higher net photosynthetic rate, but at the cost of more water loss (Matthews et al. 2017; Naumburg and Ellsworth 2000). In this study, Ci was significantly negatively correlated with WUE. Blue light promoted Ci and Gs of P.bournei seedlings, resulting in the highest Pn and Tr, while the WUE of leaves was lower under blue light. It indicated that blue light enhanced the ability of carbon dioxide in and out of P. bournei leaves, thus improved the photosynthetic rate, but also caused more water loss, which confirmed the previous conclusions. Under red and blue light treatment, Ci concentration was the lowest and WUE was the highest, while Pn and Tr were second only to blue light treatment. The results showed that the seedlings used carbon dioxide and water more effectively and harvested a good photosynthetic rate under red-blue light. Therefore, we speculated that the leaves of P.bournei seedlings treated with red-blue light had higher photosynthetic potential and photosynthetic utilization efficiency, as well as stronger photosynthetic utilization ability, which was consistent with the results of Fu et al. (2016) and Han et al. (2015).
Photoprotection mechanism and light energy distribution model
Chlorophyll fluorescence refers to the re-emission of light by chlorophyll molecules from the excited state to the non-excited state, which is used to regulate the photosynthetic energy conversion of plant leaves (Maxwell and Johnson 2000). The study of Lysenko et al. (2020) showed that the absorbed light energy was redistributed from the upper thin mesophyll layer to the lower cell layer throughout the entire leaf. The green light sensing system can harmoniously regulate plant growth and development with red and blue light sensors (Folta and Maruhnich 2007). Blue/red light is mainly absorbed by the upper layer cells, while the lower layer cells by low radiation to emit low fluorescence and have little influence on the upper layer saturation (Lysenko et al. 2020). The upper layer cells exhibit weak absorption of green light, while the lower layer consists of thick tissues that predominantly absorb green light and emit fluorescence (Smith et al. 2017; Nishio 2000). The fluorescence can enter the upper cells upward, fully affecting their saturation (Smith et al. 2017; Nishio 2000). Therefore, green light can better induce chlorophyll fluorescence kinetic behavior, thus significantly increases Fv/Fm and Fv/Fo. Kalaji et al. (2017) and Li et al. (2021b) found that F'v /F'm and ΦPSII levels were higher in plants under red-blue light, then leaf openness and electron transfer efficiency of PSII were improved. Therefore, more electrons can be absorbed, captured and transferred, which improves the efficiency of excitation energy captured by PSII reaction center and enhances the resistance of plants to photoinhibition (Li et al. 2021b). In this study, Fm, Fv/Fm and Fv/Fo were the highest under green light treatment, followed by Fv/Fm and Fv/Fo under red-blue light treatment, which was consistent with previous research conclusions. Therefore, this further indicated that the efficiency of capturing excitation energy of the PSII reaction center in leaves treated with green light was higher than that of red-blue light, that is, the inherent light energy conversion ability and potential light energy conversion efficiency were high, and green light may play a special role in regulating photosynthetic characteristics of plants (Li et al. 2021a; Su et al. 2014). Under the same PAR condition, the qP of red-blue light treatment was the highest, indicating that the PSII reaction center was more open. In the PAR-NPQ fitting curve, the NPQ of CK was significantly higher than that of other light quality treatments, followed by red-blue and blue light treatments, while red and green light treatments were lower. It showed that the light energy absorbed by the leaves under white light was mostly dissipated in the form of heat. However, red light and green light treatments have poor photoprotection ability, which may be damaged by light. Red-blue light and blue light treatments' leaves have strong heat dissipation capacity. Even if the light intensity received by the plant is excessive, they can also protect themselves by dissipating excess light energy into heat.
The results showed that in the PAR-ETR fitting curve, the Jmax was the highest under red-blue light, while PARsat ranked second only to white light. Furthermore, the initial slope (α) was also observed to be higher. Red-blue light can rapidly increase ETR and α, enhance PSII antenna pigment absorption and light energy utilization and ETR activity, improve the efficiency of light energy utilization of photosynthetic organs. This confirmed the previous speculation that red-blue light treatment has a stronger ability to utilize photosynthetic. The light energy absorbed by the PSII reaction center is used for photosynthesis, regulatory energy dissipation and non-regulatory energy dissipation, respectively. Y(II) + Y(NPQ) + Y(NO) = 1. Y(II) represents the actual photosynthetic efficiency; that is, the actual efficiency of light energy conversion. The actual photosynthetic efficiency of blue light and red-blue light treatments was higher. The difference was that the blue light treatment allocated a larger proportion of light energy to Y(NO), while the red-blue light treatment allocated more light energy to Y(NPQ). It showed that although P. bournei received excess light energy under red-blue light, it could still avoid photoinhibition and damage to the system through regulated energy consumption (Wang et al. 2022). The blue light treatment had a higher Y(NO), indicating that the energy regulation mechanism of blue light treatment was to obtain as significant Y(II) as possible by maintaining a higher Y(NO) ratio, so as to adapt to changes of light intensity. Therefore, the light damage of blue light treatment consumed more energy, and the light energy utilization efficiency was low. Red-blue light treatment achieved the best photosynthetic efficiency, heat dissipation and light protection ability was also better.
Conclusion
The impacts of varying light qualities on photosynthetic pigment and its proportion, activities of photosynthetic key enzymes, gas exchange parameters and chlorophyll fluorescence parameters in P. bournei seedlings were significant different. The blue light significantly promoted the photosynthetic pigments, activities of photosynthetic key enzyme and photosynthetic rate (Pn), while the water use efficiency (WUE) was lower. Green light treatment had the maximum potential photochemical efficiency (Fv/Fo) and maximum photochemical efficiency (Fv/Fm). Red light treatment inhibited the photosynthetic performance of P. bournei. The combination of red light and blue light was very important for the growth and development of P. bournei seedlings, and the proper ratio of red, blue and green light can better meet the needs of the seedlings for light resources. Therefore, we will further explore the appropriate ratio of red, blue and green light of P. bournei during the stage of seedlings in the future, so as to accurately regulate the spectral demand of the growth and development of P. bournei seedlings, which is conducive to the efficient cultivation of high-quality P. bournei seedlings.
Data availability
The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding authors.
Abbreviations
- Chla :
-
Chlorophyll a
- Chlb :
-
Chlorophyll b
- Ci:
-
Intercellular CO2 concentration
- CK:
-
Control treatment
- ETR:
-
Photosynthetic Electron Transfer Rate
- Fo:
-
Initial fluorescence
- Fm:
-
Maximum fluorescence
- Fv/Fm:
-
Maximum photochemical efficiency
- Fv/Fo:
-
Potential photochemical efficiency
- Gs:
-
Stomatal conductance
- J max :
-
Maximum electron transfer rate
- NPQ:
-
Non-photochemical quenching coefficient
- PARsat :
-
Saturated light intensity
- Pn:
-
Photosynthetic rate
- PSII:
-
Photosystem II
- qP:
-
Photochemical quenching coefficient
- RCA:
-
Rubisco activase
- Rubisco:
-
Ribulose-1,5-bisphosphate carboxylase/oxygenase
- Tr:
-
Transpiration rate
- WUE:
-
Water use efficiency
- Y(NO):
-
Quantum yield of non-regulated energy dissipation
- Y(NPQ):
-
Quantum yield of regulated energy dissipation
- Y(II):
-
Actual photosynthetic efficiency
- α :
-
Initial slope
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This work was supported in full by grants from the National Natural Science Foundation of China (31870613) and Guizhou Province High-level Innovative Talents Training Plan Project (2016) 5661.
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All authors contributed to the study conception and design. WXL and JNQ designed the experiment, JNQ, NRX and CXY coordinated the experiment. NRX performed statistical analysis and formulated the manuscript. NRX, SSC and WXL revised and improved the manuscript.
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Nie, R., Wei, X., Jin, N. et al. Response of photosynthetic pigments, gas exchange and chlorophyll fluorescence parameters to light quality in Phoebe bournei seedlings. Plant Growth Regul 103, 675–687 (2024). https://doi.org/10.1007/s10725-024-01131-3
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DOI: https://doi.org/10.1007/s10725-024-01131-3