Abstract
Ascorbate (AsA) is an important antioxidant that can scavenge reactive oxygen species to protect plant cells against oxidative stress. Guanosine 5'-diphosphate (GDP)-L-galactose phosphorylase (GGP) is a key enzyme in the AsA biosynthetic pathway. To investigate the functions of GGP in AsA synthesis and oxidative stress tolerance in tomato, antisense lines with a reduced expression of SlGGP were obtained. Photobleaching after treatment of leaf disks with methyl viologen was more severe in transgenic lines compared to wild type (WT) plants. Moreover, compared with the WT plants, the transgenic plants showed a higher content of hydrogen peroxide, superoxide anion, malondialdehyde, as well as ion leakage, but a lower content of AsA and chlorophylls, ascorbate peroxidase activity, net photosynthetic rate, and maximal photochemical efficiency of photosystem II. Results of real-time quantitative polymerase chain reaction show that suppression of the SlGGP gene in the transgenic plants reduced their oxidative stress tolerance.
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Abbreviations
- APX:
-
ascorbate peroxidase
- AsA:
-
ascorbate
- CAT:
-
catalase
- DHA:
-
dehydroascorbate
- D-Man/L-Gal:
-
D-mannose/L-galactose
- Fv/Fm :
-
variable to maximum chlorophyll fluorescence ratio (maximal photochemical efficiency of photosystem II)
- GDP:
-
guanosine 5'-diphosphate
- GGP:
-
GDP-L-galactose phosphorylase
- MDA:
-
malondialdehyde
- MV:
-
methyl viologen
- O2 •− :
-
superoxide anion radical
- PFD:
-
photon flux density
- PN :
-
net photosynthetic rate
- PS II:
-
photosystem II
- REC:
-
relative electric conductance
- ROS:
-
reactive oxygen species
- SlGalLDH :
-
L-galactono-1,4-lactone dehydrogenase
- SOD:
-
superoxide dismutase
- WT:
-
wild type
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Acknowledgements: This research was supported by the State Key Basic Research and Development Plan of China (2015CB150105), the Natural Science Foundation of China (31171474, 31371553), and the Research Award Foundation for Outstanding Young Scientist of Shandong Province (2014BSB01031). The first two authors contributed equally to this work.
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Yang, DY., Ma, NN., Liu, ZM. et al. Suppression of tomato SlGGP aggravates methyl viologen-mediated oxidative stress. Biol Plant 60, 677–685 (2016). https://doi.org/10.1007/s10535-016-0628-0
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DOI: https://doi.org/10.1007/s10535-016-0628-0