Abstract
This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using the TKSA-MC program based on the optimization of surface charge-charge interactions and were mutated to glutamate for shifting the enzyme’s isoelectric point off its optimum pH value. Three mutants K75E, K363E and K420E showed enhanced thermostability. The triple mutant, K75E/K363E/K420E, was found to be the best with a 7.3-fold increase in half-life (t1/2) at 33 °C compared to that of the wild-type (WT). Most importantly, this mutant showed comparable enzymatic activity to that of the WT protein. Structural modelling demonstrated that increased surface charge-charge interactions and optimization of surface hydrophobic and electrostatic contacts contributed to the improved thermostability displayed by K75E/K363E/K420E.
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Introduction
Microbial dextran is a homoglycan of α-d-glucopyranose molecule connected primarily by α-1,6 linkages, with various extents of branches via -1,2, -1,3, or -1,4 linkages to the main chain. Dextranases hydrolyze dextran to oligosaccharides and are widely used in pharmaceutical, sugar, and dental industries (Khalikova et al. 2005). In sugar industry, dextranase is used to reduce the viscosity and to increase sucrose recovery of juices (Jiménez 2009). In pharmaceutical industry, dextranase can be used with commercial dextran to produce low molecular weight clinical grade dextran as plasma substitutes (Wu et al. 2011) or isomaltooligosaccharides as prebiotics (Gan et al. 2014; Goulas et al. 2004). In dental industry, dextranase is added to oral care products, such as toothpaste and mouthwash, for degrading dextran in dental plaques and dislodging biofilm produced by Streptococcus mutans, a primary pathogen of dental caries (Pleszczynska et al. 2017).
Dextranases are classified into two broad categories, endo- and exo-dextranases, depending on their action patterns on dextran. Three types of exo-dextranases have been reported, glucodextranase (also referred to as dextran glucosidase, EC 3.2.1.70) (Linder and Sund 1981; Mizuno et al. 2004; Morimoto et al. 2004; Saburi et al. 2015; Takahashi 1982), isomalto-dextranase (EC 3.2.1.94) (Okazawa et al. 2015) and isomaltotrio-dextranase (EC 3.2.1.95) (Mizuno et al. 1999). These exo-dextranases hydrolyze dextran from the non-reducing end to produce glucose, isomaltose and isomaltotriose, respectively. We have expressed and characterized an α-glucoside hydrolase, PspAG97A, produced by the deep-sea bacterium Pseudoalteromonas sp. K8 (Li et al. 2016). PspAG97A represents the first reported glucodextranase from marine bacteria that belongs to glycoside hydrolase GH97 family with high catalytic efficiency for dextran and unique features, like alkaline-adaption, moderate-temperature catalysis and halophilic. When p-nitrophenyl α-d-glucopyranoside was used as substrate, PspAG97A showed the highest activity at pH 7.5 and 30 °C in 0.8–1 M NaCl. Dextranases working efficiently under both alkaline and moderate-temperature conditions have been seldomly reported (Khalikova et al. 2005). PspAG97A from marine microorganism was an exception and may have application potential in dental products since alkaline oral care products are more protective to enamel than acidic products (Majeed et al. 2011). However, the relative instability and moderate rate of inactivation (maintaining only 50% of its activity after incubation at 30 °C for 7 h) severely limits the industrial applications of PspAG97A.
The crystal structure of PspAG97A (He et al. 2016) makes it possible to improve the thermostability of this unique dextranase by rational design. PspAG97A possesses an isoelectric point (pI) of 7.33, propinquant to the enzyme’s optimum pH of 7.5. The close proximity of the enzyme’s pI to its optimum pH generally prevents the application of the protein at optimum condition due to protein aggregation and instability. On the other hand, Makhatadze et al. has explored the optimization of the energy of the charge-charge interactions on protein surface via the Tanford–Kirkwood solvent accessibility (TKSA) model (Makhatadze 2017). Based on this model, optimization of charge-charge interactions via directed mutations has successfully enhanced the thermal stability of many different proteins (Gribenko et al. 2009; Tu et al. 2015). However, this strategy has not been applied to a dextran glucosidase. Therefore, we have constructed several mutants of PspAG97A using a free web server that calculates protein charge-charge interactions via the TKSA model with the Monte Carlo sampling method (Contessoto et al. 2018). All three single point mutants, K75E, K363E and K420E, showed enhanced thermostability. The triple mutant, K75E/K363E/K420E, was found to be the best with a 7.3-fold increase in half-life (t1/2) at 33 °C and essentially identical activity compared to that of the WT protein. This work demonstrates the successful application of rational design guided by the TKSA-MC model in improving the stability and activity of target proteins.
Materials and methods
Selection of mutation sites and site-directed mutagenesis
Charge-charge interaction energy ΔGqq for each ionizable residue was calculated by the TKSA-MC model (Contessoto et al. 2018) with input file of PDB ID: 5HQ4 and input parameters of pH 7.5 and temperature 308 K. The recombinant plasmid with the wild-type PspAG97A gene (residues 20–680, without the predicted N-terminal signal peptide), pET-28a-pspag97a, was used as the template. Site-directed mutagenesis was conducted using the Site Directed Mutagenesis Kit (TaKaRa) according to the manufacturer’s instructions. Once the sequence of the mutant enzyme gene was confirmed by sequencing, the expression plasmids were transformed into E. coli BL21 (DE3).
Protein expression and purification
The transformant was cultured in LB medium at 37 °C until OD600 reached about 0.8. Protein expression was induced with 0.1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) at 16 °C for 24 h. The cell pellet was collected by centrifugation of the culture media at 4000 rpm for 10 min and resuspended and disrupted in buffer A (20 mM Tris–HCl, pH 8.5, 500 mM NaCl). After centrifugation, the supernatant was loaded onto a HisTrap nickel-Sepharose column (GE Healthcare), and the protein was eluted with buffer A containing a step gradient of 50–250 mM imidazole. The purified protein was then concentrated for subsequent analysis. Protein purity was examined by SDS-PAGE and protein concentration was determined by the Bradford protein assay using bovine serum albumin as the standard.
Dextran substrate specificity
For substrate specificity of PspAG97A, we investigated the activities by using 5% (w/v) commercial dextrans T5, T10, T20, T40 and T70 (obtained from Shanghai Aladdin Bio-Chem Technology Co., LTD) in buffer B (50 mM Na2HPO4–KH2PO4, pH 7.5, 800 mM NaCl). After incubation at 35 °C for 5 min, the reaction was quenched with boiling water and centrifugation. The amount of glucose released was colorimetrically determined using the Glucose Assay kit (Shanghai Rongsheng Biological Pharmaceutical Co., Ltd.) at 505 nm. Glucose was used as the standard. One unit of activity was defined as the amount of enzyme required to liberate 1 μmol of glucose per minute at 35 °C and pH 7.5. Relative activity was expressed as percentage of the highest activity (100%) toward T5. All assays were conducted in triplicate.
Effects of temperature on activity and stability of enzymes
The optimal temperature of the enzymes was determined by measuring the enzyme activity at different temperatures (10–50 °C) in buffer B, using 5% (w/v) T5 as substrate. Relative activity was expressed as percentage of the highest activity (100%). All assays were conducted in triplicate. To assess thermal stability, the enzyme solution was pre-heated at 35 °C or 33 °C for different times and rapidly cooled. The residual activity was measured using the assay described above.
Effects of pH on enzyme activity
To determine the optimal pH of the enzymes, activity was measured in buffers of different pH containing 800 mM NaCl and 5% (w/v) dextran T5 at 35 °C. Solutions with different pH values were used as follows: 50 mM Na2HPO4-KH2PO4 buffer (pH 6.0–7.0) and 50 mM Tris–HCl buffer (pH 7.0–9.0). Relative activity was expressed as percentage of the highest activity (100%). All trials were conducted in triplicate.
Products of enzyme hydrolysis
Recombinant WT PspAG97A and K75E/K363E/K420E mutant catalyzed hydrolysis of 5% (w/v) dextran T5 took place at 35 °C in buffer B. For TLC analysis, reaction products were developed twice on a silica gel plate (Haiyang Chemical Co., Ltd, Qingdao, China) with a mobile phase consisting of trichloromethane/acetic acid/water (4:9:1 by volume) and then visualized using sulfuric acid/ethanol (1:9 by volume), followed by heating the plate at 80 °C for 5 min.
Determination of kinetic parameters
Kinetic parameters of purified WT PspAG97A and its mutant enzymes were determined with 5% (w/v) dextran T5 at 35 °C in buffer B. The initial velocity was determined by plotting absorbance versus time for each substrate concentration. The kcat, Km, and kcat/Km were calculated by fitting the data to the Michaelis–Menten equation using nonlinear regression with Origin.
Structural analysis
The structural model of the K75E/K363E/K420E mutant was generated on the basis of the PspAG97A structure (PDB ID: 5HQ4) by Modeller 9.16 (Webb and Sali 2016). The PyMol Molecular Graphics System (DeLano Scientific LLC, San Carlos, CA, USA) was used for the visualization and analysis of the protein structure.
Circular dichroism (CD)
Far-UV CD spectra were recorded at 25 °C with circular dichroism spectrometer (Applied Photophysics) between 180 and 260 nm using a 1 mm path length cell and approximately 500 μg/ml protein sample in buffer B. A buffer only reference was subtracted from each curve.
Fluorescence spectroscopy
Intrinsic fluorescence measurements of the proteins were recorded at 25 °C with a Shimadzu RF 5301 spectrofluorophotometer in a 0.1 cm path length quartz cell using approximately 200 μg/mL protein sample in buffer B. Fluorescence emission spectra were recorded from 300 to 450 nm using an excitation wavelength of 280 nm.
Results
PspAG97A hydrolyzes various molecular weight dextrans
The activities of PspAG97A in the catalyzed hydrolysis of dextrans of different molecular weights were determined. PspAG97A showed the highest activity at 35 °C and pH 7.5 with dextran T5, which has an average molecular weight of 5 kDa (Table 1). As the molecular weight of the dextran substrate increased, the specific activity of the enzyme decreased gradually. The highest specific activity toward 5% (w/v) T5 was about 1086 U/mg. When the specific activity toward T5 was taken as 100%, this enzyme displayed about 97.1%, 77.75%, 67.15% and 62.3% relative activities toward 5% (w/v) T10, T20, T40 and T70 with average molecular weights of 10, 20, 40 and 70 kDa, respectively. These results showed that PspAG97A can hydrolyze dextrans of various molecular weights with the highest activity toward 5% (w/v) T5, the substrate used in the following activity and thermostability assays.
Choice of residues for site-directed mutagenesis
Lysine residues are the most abundant among all positively charged residues present on the surface of PspAG97A. Compared to arginine residues, lysine residues participate less often in multipoint electrostatic interactions. Thus, it was decided to decrease the isoelectric point by substituting lysine residues on the surface of the protein. The TKSA-MC analysis (Contessoto et al. 2018) allows the calculation of the charge-charge interaction free energy (ΔGqq, Fig. 1a) of each ionizable residue. The bars indicate the ΔGqq contribution of each ionizable residue to the stability of protein’s native state when compared to the unfolded state. The red bars indicate the candidate residues to be mutated to increase protein stability. For PspAG97A at the optimal condition (pH 7.5 and 35 °C), the highlighted fourteen residues are D26, E36, K75, K135, K281, D354, K363, K366, K420, K447, K449, K595, K598, D622, eleven of which are lysine (Fig. 1a). Moreover, the candidate for lysine substitution should not be conserved. Multiple sequence alignment revealed that K281 and K447 are relatively conserved in GH97 inverting subfamily (Fig. 1b). Based on the above analysis, nine lysine residues at positions 75, 135, 281, 363, 366, 420, 449, 595 and 598 were chosen as targets for introducing mutations. As K281 presents the highest unfavorable ΔGqq, it was also included as a target despite being conservative. The negatively charged glutamate was chosen to replace the positively charged lysine residue(s) to achieve the expected dramatic reduction in the enzyme’s pI value.
Thermostability assays of WT and the nine single point mutants
The genes encoding the WT and mutant enzymes were cloned and expressed in E. coli BL21(DE3). After purification by Ni-affinity chromatography, each of the mutant proteins migrated as a single band and displayed the same molecular weight as WT on SDS-PAGE (Supplementary Fig. 1). PspAG97A showed poor thermostability at its optimum temperature of 35 °C, with only 57.8% activity retained after incubation for 20 min (Fig. 2a). We then selected a lower temperature 33 °C to determine the thermostability of the WT and mutant enzymes. As shown in Table 2 and Fig. 2b, the t1/2 values at 33 °C for the K363E, K420E and K75E mutants were about 26 h, 17 h and 14 h, respectively, higher than that for the WT (only 6 h). The other six mutants, K135E, K281E, K366E, K449E, K595E and K598E, showed similar or lower t1/2 values than that for the WT. In addition, the three thermostability-enhanced single point mutants had similar optimum temperature and pH (35 °C and pH 7.5–8.0) (Table 2), all retaining more than 80% activity within the ranges of 35–40 °C and pH 7.0–8.0 (Supplementary Fig. 2).
Construction of double and triple mutants and their thermostability assays
To further improve the thermostability of PspAG97A, we performed multiple mutations by constructing three double mutants K75E/K363E, K75E/K420E and K363E/K420E, and one triple mutant K75E/K363E/K420E. The theoretical pI value was reduced from 7.33 to 6.44 for the triple mutant K75E/K363E/K420E. All mutants had good protein purity (Supplementary Fig. 3). As shown in Table 2 and Fig. 2c, the t1/2 values at 33 °C for K75E/K420E, K75E/K363E, K363E/K420E and K75E/K363E/K420E mutants were 24, 32, 36 and 44 h, respectively, significantly higher than that of the WT protein. The triple mutant also showed improved thermostability at 35 °C with 58.8% activity retained after 90 min incubation (Fig. 2a). In addition, the four combinational mutants had similar optimum temperature and pH (40 °C and pH 7.0–7.5) (Table 2), all retaining more than 80% activity within the ranges of 35–40 °C and pH 7.0–8.0 (Supplementary Fig. 4). These results demonstrated that the triple mutant K75E/K363E/K420E was the best in terms of improving the thermostability of PspAG97A.
Kinetic parameters and hydrolysis products of WT and the mutants
The kinetic parameters of the WT and thermostability-enhanced mutants were determined at 35 °C and pH 7.5 using ten different substrate concentrations of dextran T5. As shown in Table 3, the Km, kcat and kcat/Km values of these mutants were almost similar to that of WT, suggesting that although these mutations improved the thermostability of PspAG97A, they did not affect the binding affinity between protein and substrate, the turn-over number, and the catalytic efficiency of the enzyme. Moreover, the hydrolysis products catalyzed by WT and the best performing mutant K75E/K363E/K420E were analyzed through TLC with 5% dextran T5 as substrate at 35 °C and pH 7.5 for different periods (10 min, 20 min and 30 min). As the reaction progressed, the amount of glucose produced increased (Fig. 3), confirming that both PspAG97A WT and its best performing mutant are glucose-forming exodextranases.
Structural modelling and explanation for the improved thermostability
To explain why thermostability was enhanced for these mutants, the overall structures of the thermostability-enhanced mutants were compared with that of the WT protein. The CD spectra of these mutants and WT were similar (Fig. 4a), indicating no significant secondary structural changes were induced by the mutations. In addition, fluorescence spectra showed that these mutants and WT exhibited the maximum emission at the same wavelength of 340 nm (Fig. 4b), suggesting that the overall tertiary structures of these mutants remained similar as that of the WT. Structural modelling of the mutant K75E/K363E/K420E was then performed. According to the model, the hydrophobic and electrostatic interactions between residue-75 and its surrounding residues Gln77 and Thr74 are likely optimized for the K75E mutation (Fig. 5a, b). Additional electrostatic interactions might be formed between E363 and K366 (Fig. 5c, d), and between E420 and R417 (Fig. 5e, f). Therefore, we postulated that additional charge-charge interactions and optimization of surface hydrophobic and electrostatic contact resulted in the improved thermostability of the K75E/K363E/K420E mutant.
Discussion
In enzyme engineering, it is important to retain the enzyme’s activity when manipulating enzyme stability. Some earlier attempts to improve enzyme thermostability have resulted in the reduction of enzyme activity (Guo et al. 2015; Wang et al. 2014). In this study the enzyme stability was increased via optimization of surface charge-charge interactions using the TKSA-MC model (Contessoto et al. 2018), combined with alteration of the enzyme’s isoelectric point. Consequently, the mutants generated in this study displayed improved thermostability without compromising the catalytic activity of the WT protein. In addition, earlier studies have shown that beneficial amino acid mutations commonly show additive (Lin et al. 2018) or sometimes asynergistic effect (Wang et al. 2018) on the protein’s thermostability. In this study, beneficial amino acid mutations of K75E, K363E and K420E exhibited synergistic effect on the thermostability of the target protein.
The best performing mutant, K75E/K363E/K420E, of the glucodextranase PspAG97A not only shows improved thermostability (remaining 58.8% activity at 35 °C for 90 min compared to the WT remaining 57.8% activity at 35 °C for 20 min), but also retains its original unique properties, such as high specific activity for dextran (about 1000 U/mg for dextran T5 and 680 U/mg for T70), alkaline-adaption (the optimum pH 7.0–7.5 and retaining more than 80% activity within the range of 7.0–8.0), moderate-temperature catalysis (the optimum temperature 40 °C and retaining more than 80% activity within the range of 35–40 °C) and halophilic (the highest activity at 0.8–1 M NaCl and retaining about 15% activity at physiological 0.15 M NaCl). This thermostability-improved mutant of PspAG97A would be more suitable for application in dental products due to the following reasons. As mentioned before, alkaline oral care products are more protective to enamel than acidic products. However, dextranases reported till now usually function under acidic and mega-thermal conditions (Khalikova et al. 2005). Among them, only a few have been characterized as exo-dextranases (Li et al. 2016; Linder and Sund 1981; Mizuno et al. 1999, 2004; Morimoto et al. 2004; Okazawa et al. 2015; Saburi et al. 2015; Takahashi 1982). To our knowledge, except for PspAG97A (Li et al. 2016) and an isomaltotriose-forming dextranase from Brevibacterium fuscum (Mizuno et al. 1999), all reported exo-type dextranases have the optimum pH lower than 7.5. Second, salt (NaCl) has antimicrobial properties (Wijnker et al. 2006) and has been added to some oral care products (Sidhu et al. 2014). Michel et al. evaluated the effectiveness of sea salt-containing mouth rinse and noted a decrease in gingival and periodontal indices at the end of the trial period during which each child rinsed with a solution containing 2.5 g of sea salt in 20 ml of water (Michel et al. 2013). Mani et al. reported a significant reduction in all clinical parameters in 30 adults with gingivitis after mouth rinsing with sea salt for a period compared to those who did not use the rinse (Mani et al. 2015). Therefore, a halophilic dextranase might be better compatible with salt-containing oral care products. Third, this mutant has optimum temperature of 40 °C compared to 35 °C for the WT, and shows improved thermostability within the range of 33.2–38.2 °C in oral temperature (Sund-Levander et al. 2002). Recently, Wang et al. successively reported two alkaline and cold-adapted endo-type dextranases from marine bacteria, suitable for development of dental products (Lai et al. 2019; Ren et al. 2018). In this study, we reported a thermostability-improved alkaline-adapted, mesophilic and halophilic exo-type dextranase from marine bacteria that might have application potential in dental products in combination with alkaline-adapted endo-type dextranases.
In conclusion, the thermostability of an alkaline-adapted, mesophilic and halophilic dextran glucosidase, PspAG97A, was remarkably improved via the optimization of surface charge-charge interaction combined with the shift of the enzyme’s pI off its optimum pH value. The best combinational mutant, K75E/K363E/K420E, showed a 7.3-fold increase in the half-life (t1/2) at 33 °C compared to that of the WT without compromising the catalytic activity of the WT protein.
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Acknowledgements
This work was supported by National Natural Science Foundation of China (No. 31570064), and the Key Research Program of the Education Department of Anhui Province (No. KJ2018A0002).
Supporting information
Supplementary Figure 1—SDS-PAGE analysis of the recombinant PspAG97A enzyme and its nine single point mutants. M: protein marker; Lane 1–10: PspAG97A, K75E, K135E, K281E, K363E, K366E, K420E, K449E, K595E, K598E
Supplementary Figure 2—Effect of temperature (A–D) and pH (E–H) on enzyme activities of WT PspAG97A and its three thermostability-enhanced single point mutants.
Supplementary Figure 3—SDS-PAGE analysis of the recombinant PspAG97A enzyme and its combinational mutants. M: protein marker; Lane 1–5: PspAG97A, K75E/K363E, K75E/K420E, K363E/K420E, K75E/K363E/K420E.
Supplementary Figure 4—Effect of temperature (A–D) and pH (E–H) on enzyme activities of four thermostability-enhanced multiple point mutants of PspAG97A.
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Zhang, X., Chen, F., He, C. et al. Improving the thermostability of a GH97 dextran glucosidase by rational design. Biotechnol Lett 42, 2211–2221 (2020). https://doi.org/10.1007/s10529-020-02928-8
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DOI: https://doi.org/10.1007/s10529-020-02928-8