We studied the effect of selective α2C-adrenergic receptor antagonist JP-1302 in concentrations of 10—9-10—6 M on inotropy, chronotropy, and coronary flow in the Langendorff-isolated rat heart. JP-1302 in all studied concentrations decreased the left-ventricular myocardium force contraction, HR, and coronary flow. The maximum inotropic, chronotropic, and vascular effects were observed when the antagonist was applied to the perfused solution in a concentration of 10—7 M. The least pronounced decrease in the studied parameters was observed at JP-1302 concentrations of 10—8 and 10—9 M. The obtained data indicate the participation of this subtype of α2-adrenergic receptors in the regulation of activity of isolated adult rats heart.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Brodde OE, Bruck H, Leineweber K. Cardiac adrenoceptors: physiological and pathophysiological relevance. J. Pharmacol. Sci. 2006;100(5):323-337. doi: https://doi.org/10.1254/jphs.crj06001x
Civantos Calzada B, Aleixandre de Artiñano A. Alpha-adrenoceptor subtypes. Pharmacol. Res. 2001;44(3):195-208. doi: https://doi.org/10.1006/phrs.2001.0857
El-Ayoubi R, Menaouar A, Gutkowska J, Mukaddam-Daher S. Imidazoline receptors but not alpha 2-adrenoceptors are regulated in spontaneously hypertensive rat heart by chronic moxonidine treatment. J. Pharmacol. Exp. Ther. 2004;310(2):446-451. doi: https://doi.org/10.1124/jpet.104.067595
Porter AC, Svensson SP, Stamer WD, Bahl JJ, Richman JG, Regan JW. Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes. Life Sci. 2003;72(13):1455-1466. doi: https://doi.org/10.1016/s0024-3205(02)02381-0
Brodde OE, Michel MC. Adrenergic and muscarinic receptors in the human heart. Pharmacol. Rev. 1999;51(4):651-690.
Maltsev AV, Kokoz YM, Evdokimovskii EV, Pimenov OY, Reyes S, Alekseev AE. Alpha-2 adrenoceptors and imidazoline receptors in cardiomyocytes mediate counterbalancing effect of agmatine on NO synthesis and intracellular calcium handling. J. Mol. Cell. Cardiol. 2014;68:66-74. doi: https://doi.org/10.1016/j.yjmcc.2013.12.030
Gyires K, Zádori ZS, Török T, Mátyus P. alpha(2)-Adrenoceptor subtypes-mediated physiological, pharmacological actions. Neurochem. Int. 2009;55(7):447-453. doi: https://doi.org/10.1016/j.neuint.2009.05.014
Philipp M, Hein L. Adrenergic receptor knockout mice: distinct functions of 9 receptor subtypes. Pharmacol. Ther. 2004;101(1):65-74. doi: https://doi.org/10.1016/j.pharmthera.2003.10.004
Maltsev AV, Evdokimovskii EV, Kokoz YM. α2-adrenoceptor signaling in cardiomyocytes of spontaneously hypertensive rats starts to impair already at early age. Biochem. Biophys. Res. Commun. 2019;512(4):908-913. doi: https://doi.org/10.1016/j.bbrc.2019.03.117
Evdokimovskii EV, Jeon R, Park S, Pimenov OY, Alekseev AE. Role of α2-adrenoceptor subtypes in suppression of L-type Ca2+ current in mouse cardiac myocytes. Int. J. Mol. Sci. 2021;22(8):4135. doi: https://doi.org/10.3390/ijms22084135.
Zefirov TL, Khisamieva LI, Ziyatdinova NI, Zefirov AL. Selective blockade of α2-adrenoceptor subtypes modulates contractility of rat myocardium. Bull. Exp. Biol. Med. 2016;162(2):177-179. doi: https://doi.org/10.1007/s10517-016-3569-x
Zefirov TL, Khisamieva LI, Ziyatdinova NI, Zefirov AL. Effect of selective blockade of α2C-adrenoceptors on cardiac activity in growing rats. Bull. Exp. Biol. Med. 2015;159(6):697-699. doi: https://doi.org/10.1007/s10517-015-3051-1
Brede M, Nagy G, Philipp M, Sorensen JB, Lohse MJ, Hein L. Differential control of adrenal and sympathetic catecholamine release by alpha 2-adrenoceptor subtypes. Mol. Endocrinol. 2003;17(8):1640-1646. doi: https://doi.org/10.1210/me.2003-0035
Moura E, Afonso J, Hein L, Vieira-Coelho MA. Alpha2-adrenoceptor subtypes involved in the regulation of catecholamine release from the adrenal medulla of mice. Br. J. Pharmacol. 2006;149(8):1049-1058. doi: https://doi.org/10.1038/sj.bjp.0706950
Pimenov OY, Galimova MH, Evdokimovskii EV, Averin AS, Alekseev AE, Nakipova OV, Reyes S. Myocardial α2-adrenoceptors as therapeutic targets to prevent cardiac hypertrophy and heart failure. Biophysics. 2019;64(5):738-750. doi: https://doi.org/10.1134/S000635091905021X
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 5, pp. 545-548, May, 2023
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Zefirov, T.L., Khisamieva, L.I., Khabibrakhmanov, I.I. et al. α2C-Adrenergic Receptor Blockade Inhibits Langendorff-Isolated Rat Heart Work. Bull Exp Biol Med 175, 612–615 (2023). https://doi.org/10.1007/s10517-023-05911-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-023-05911-1