Abstract
Folate-containing dextran ligand (FA-Dextran-DTPA) was synthesized by the incorporation of diethylenetriaminepentaacetic acid (DTPA) and folate (FA) as a tumor-targeting group into dextran as a polymer carrier. This ligand was further reacted with gadolinium chloride to make a dextran gadolinium complex FA-Dextran-DTPA-Gd. The ligand and its gadolinium complex were characterized by 1H-NMR, FTIR, UV-Vis, average particle sizes and zeta potential, as well. In vitro properties including relaxivity, cytotoxicity assay, cellular uptake assay, and magnetic resonance imaging (MRI) were also evaluated. Compared with Gd-DTPA, FA-Dextran-DTPA-Gd possessed obviously higher relaxation effectiveness and lower cytotoxicity to HeLa cells. FA-Dextran-DTPA-Gd had a high affinity to the H460 and MDA-MB-231 tumor cells and can be taken up selectively by these tumor cells. Moreover, FA-Dextran-DTPA-Gd showed enhanced signal intensities (SI) of MRI and enhanced the contrast of MR images of tumor cells. These results indicated that FA-Dextran-DTPA-Gd showed the potential as a tumor-targeting contrast agent in MRI.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Lauterbur, P.C., Nature, 1973, 242: 190
Wedeking, P., Sotak, C.H., Telser, J., Kumar, K., Chang, C.A. and Tweedle, M.F., Magn. Reson. Imaging, 1992, 10: 97
Gong, F.M., Zhang, Z.Q., Chen, X.D., Zhang, L., Yu, X.S., Yang, Q.H., Shuai, X.T., Liang, B.L. and Cheng, D., Chinese J. Polym. Sci., 2014, 32(3): 321
Villaraza, A.J.L., Bumb, A. and Brechbiel, M.W., Chem. Rev., 2010, 110: 2921
Yan, G.P., Ai, C.W., Zong, R.F. and Liu, F., Chin. Sci. Bull., 2001, 55(27–28): 3085
Yan, G.P., Hu, B., Liu, M.L. and Li, L.Y., J. Pharm. Pharmacol., 2005, 57(3): 351
Yan, G.P., Zhuo, R.X., Zhang, X., Xu, M.Y., Li, L.Y. and Ye, Z.H., Polym. Int., 2002, 51: 892
Comblin, V., Gilsoul, D., Hermann, M., Humblet, V. and Jacques, V., Coord. Chem. Rev., 1999, 185: 451
Weinmann, H.J., Brash, R.C., Press, W.R. and Wesby, G.E., Radiology, 1984, 142: 619
Wen, J., Zhuo, R.X. and Wang, L., Chin. J. Magn. Reson., 1998, 15(3): 217
Driksen, A., Langereis, S., de Waal, B.F.M., van Genderen, M.H.P., Meijer, E.W., de Lussanet, Q.G. and Hackeng, T.M., Org. Lett., 2004, 6(26): 4857
Bligh, S.W.A., Chowdhury, A.S., Kennedy, D., Luchinat, C. and Parigi, G., Magn. Reson. Med., 1999, 41: 767
Yan, G.P. and Zhuo, R.X., Chin. Sci. Bull., 2001, 46(15): 1233
Zhou, X., Yang, L., Yan, G.P., Xu, W., Zhou, C.K., Zhang, Q., Li, L., Liu, F., Guo, J.F. and Zhao, Q., J. Rare Earths, 2012, 30(9): 884
Jung, K.H., Kim, H.K., Lee, G.H., Kang, D.S., Park, J.A., Kim, K.M., Chang, Y.M. and Kim, T.J., J. Med. Chem., 2011, 54(15): 5385
Jain, T.K., Foy, S.P., Erokwu, B., Dimitrijevic, S., Flask, C.A. and Labhasetwar, V., Biomaterials, 2009, 30: 6748
Wi, X.M., Feng, Y., Jeong, E.K., Mohs, A.M., Emerson, L. and Lu, Z.R., Pharm. Res., 2009, 26: 2202
Yu, K.C., Hu, H.B., Liu, M.L., Yuan, H.Z., Ye, C.H. and Zhuo, R.X., Chinese J. Polym. Sci., 1999, 17(5): 471
Yan, G.P., Zhuo, R.X., Yang, Y.H., Xu, M.Y., Li, L.Y. and Ye, Z.H., J. Bioact. Compatible Polym., 2002, 17(2): 139
Lauffer, R.B., Chem. Rev., 1987, 87: 901
Caravan, P., Ellison, J.J., Mcmurry, T.J. and Lauffer, R.B., Chem. Rev., 1999, 99: 2293
Kobayashi, H., Kawamoto, S., Star, R.A., Waldmann, T.A., Tagaya, Y. and Brechbiel, M.W., Cancer Res., 2006, 63(2): 271
Yan, G.P., Bottle, S.E., Zhuo, R.X., Wei, L., Liu, M.L. and Li, L.Y., J. Bioact. Compatible Polym., 2004, 19(6): 453
Yan, G.P., Li, Z., Xu, W., Zhang, Q., Li, L., Liu, F., Han, L., Ge, Y.X. and Guo, J.F., Int. J. Pharmaceut., 2011, 407: 119
Rinaudo, M., Polym. Int., 2008, 57(3): 397
Yan, G.P., Xu, W., Yang, L., Li, L., Liu, F. and Guo, Q.Z., Pharm. Res., 2010, 27(9): 1884
Sega, E.I. and Low, P.S., Cancer Metastasis Rev., 2008, 27: 655
Sun, X.M., Xu, J.X., Tang, J.B., Sui, M.H. and Shen, Y.Q., Chinese J. Polym. Sci., 2011, 29(4): 427
Leamon, C.P., Curr. Opin. Invest. Drugs, 2008. 9(12): 1277
Leamon, C.P., Cooper, S.R. and Hardee, G.E., Bioconjugate Chem., 2003, 14: 738
Majoros, I.J., Williams, C.R, Becker, A. and Baker, J.R.Jr., WIREs Nanomed. Nanobiotechnol., 2009, 502–510.
Shukla, S., Wu, G., Chatterjee, M., Yang, W., Sekido, M., Diop, L.A., Muller, R., Sudimack, J.J., Lee, R.J., Barth, R.F. and Tjarks, W., Bioconjugate Chem., 2003, 14: 158
Kyung, M.W., Lin, Y.H., O’Loughlin, T., Tang, Y., Kim, D.E., Weissleder, R. and Tung, C.H., Bioconjugate Chem., 2003, 14: 539
Konda, S.D., Aref, M., Wang, S., Brechbiel, M. and Wiener, E.C., Magn. Reson. Mater. Phys., Biol. Med., 2001, 12: 104
Yan, G.P., Liu, M.L. and Li, L.Y., Bioconjugate Chem., 2005, 16: 967
Yan, G.P., Shao, C.T., Han, L., Zou, T.J., Liu, F., Gong, X.Y. and Chang, X.P., Journal of Wuhan Institute of Technology, 2013, 7: 43
Shi, F.Q., Medical animal experiment method, Beijing: People’s Medical Publishing House, China, 1990
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was financially supported by the National Natural Science Foundation of China (Nos. 51173140 and 51373128), Wuhan Scientific and Technological Project (No. 2013010501010131), Wuhan Science and Technology Innovation Team of Hi-tech Industrial Project, Hubei Province (No. 2015070504020217) and Innovation Fund for Post-graduate Education (Nos. CX2014058 and CX2013083), Wuhan Institute of Technology, China.
Rights and permissions
About this article
Cite this article
Du, Hj., Shen, Yc., Liu, Yp. et al. Dextran gadolinium complex containing folate groups as a potential magnetic resonance imaging contrast agent. Chin J Polym Sci 33, 1325–1333 (2015). https://doi.org/10.1007/s10118-015-1681-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10118-015-1681-4