Abstract
The Aegean archipelago has long been the main research area of numerous biogeographers, plant ecologists and taxonomists due to its intricate palaeogeography and high environmental and topographical heterogeneity. Nevertheless, some parts of this archipelago are essentially unexplored and the processes driving spatial variation in species composition remain unaddressed. Aiming to fill these gaps, we investigated the flora and plant diversity patterns of the Northern Sporades island group, as well as its biogeographical relationships. The study area lies in the biogeographical region of the West Aegean islands and comprises 23 islands and islets. The total flora of the study area consists of 1202 infrageneric taxa, belonging to 517 genera and 120 families, reflecting its geographical and bioclimatic characteristics. The endemic element consists of 41 taxa (3.4% of the flora), eight of which are restricted to the West Aegean islands and two are single island endemics. Area emerged as the most important variable in shaping plant species richness, while niche-based processes played a lesser role in driving these patterns. Regarding the taxonomic and phylogenetic beta-diversity patterns, environmental filtering and not dispersal limitation seems to shape the plant assemblages of the Northern Sporades islets. Biogeographically, the Northern Sporades island group seems to be closer connected to the Kiklades rather than to Evvia or the adjacent mainland, due to their longer isolation and separate palaeogeographical history during the Quaternary.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Islands are biodiversity hotspots and regarded as natural laboratories because they provide natural sites for experiments on the processes of island colonization, species turnover (Darwin 1859; Greuter 1995; Denslow 2001) and represent rather simplified real-world systems (Kueffer and Fernández-Palacios 2010). Nevertheless, island insularity differs in intensity, depending on proximity to the mainland (Vogiatzakis et al. 2016) and displays more research advantages compared to the mainland, as ecological and evolutionary processes are relatively easier to detect on islands due to their geographical isolation (Whittaker and Fernández-Palacios 2007). Furthermore, islets are recognized as fragile ecosystems on which random factors, intense environmental variation or human impact can shape the insular flora and vegetation to a great extent, particularly because of the small plant population size (Panitsa et al. 2006).
The Mediterranean Basin, a terrestrial biodiversity hotspot (Médail and Quezel 1997; Myers et al. 2000; Médail and Myers 2004), is the second largest hotspot in the world (Critical Ecosystem Partnership Fund 2017) and it hosts ca. 10,000 islands and islets. It is characterized by immense biogeographical intricacy (Médail 2017), extremely high diversification rates (Rundel et al. 2016) and high endemism (Rundel et al. 2016), due to its high topographic and climate heterogeneity. Most of these endemics have a very narrow geographical range, often restricted to single islands, mountains or coastal plains, as a result of the region’s intricate geography and orography (Rundel et al. 2016 and references therein), thus leading to high taxonomic beta diversity (Thompson 2005). The European part of the Mediterranean alone harbours 10% of all known higher plants (Médail and Quezel 1997). The Aegean archipelago is an important component of the Mediterranean region (Triantis and Mylonas 2009), and its more than 8000 islands and islets, the unique biogeographical position between Africa, Europe and Asia, the high levels of environmental and topographical heterogeneity, and a complex palaeogeographic history contribute to high diversity and endemism (Triantis and Mylonas 2009; Kougioumoutzis et al. 2017), and render it an ideal stage for biodiversity and biogeographical studies (Strid 1996, 2016a, b), especially for land-bridge, continental islands (Panitsa et al. 2018).
Beta diversity compared to alpha diversity, allows testing of different hypotheses about the processes driving species distributions and biodiversity (Valli et al. 2019). In addition, comparison of the taxonomic and phylogenetic beta diversity may reveal the mechanisms that generate and maintain biodiversity, such as geographic isolation, environmental filtering and convergent adaptation (Weinstein et al. 2014). The use of phylogenies is commonly used in community ecology in order to understand the origins and histories of species within a community (i.e. alpha diversity) and explore theories about the influence of historical and ecological factors in structuring communities (Webb et al. 2002; Graham and Fine 2008).
The Aegean archipelago has fascinated many biogeographers, and many studies concerning the flora, endemism and factors affecting plant species richness in the Aegean Sea were conducted (e.g. Panitsa et al. 2006, 2010, 2018; Kallimanis et al. 2010, 2011; Kagiampaki et al. 2011; Iliadou et al. 2014a; Kougioumoutzis and Tiniakou 2014; Kougioumoutzis et al. 2014a, b, 2017). However, most of the biogeographical studies on the Aegean islands have focused on factors influencing plant diversity and endemism, either separately or in comparison with other archipelagos, while there has been limited research on the renewed emphasis on understanding spatial variation in species composition, or beta diversity (Panitsa et al. 2008; Sfenthourakis and Panitsa 2012; Valli et al. 2019). A better understanding of the environmental and geographical drivers that affect beta-diversity patterns and the way that these relationships vary between regions with different ecological and evolutionary histories may gain insights into the processes of structuring ecological communities (Kraft et al. 2011; Fitzpatrick et al. 2013) and shaping species assemblages on continental island systems (Valli et al. 2019).
However, several factors affecting the diversity and structure of Aegean island plant assemblages have yet to be addressed, such as the phylogenetic aspect of species and beta diversity. By incorporating phylogenetic information in biodiversity and biogeographical studies, the distinct roles of geohistorical and ecological processes in shaping diversity patterns and community structure may be revealed (Webb et al. 2002; Cavender-Bares et al. 2009; Siefert et al. 2013; Swenson 2013; Mazel et al. 2017). Consequently, the evolutionary relatedness of species co-occurring within and across regions can be quantified, and the community phylogenetic structure can then be associated to niche-related, neutral and historical processes that mediate species coexistence (Cavender-Bares et al. 2009; Graham et al. 2009).
The Northern Sporades island complex seems well-suited to better understand the evolutionary and ecological processes that have shaped plant assemblages within the Aegean archipelago, due to the morphology, the geographical isolation, the limited degree of human impact mainly on small islets and the environmental conditions of the Northern Sporades. The vegetation of the islands is mainly composed of evergreen sclerophyllous and phryganic plant communities, while halophytic and chasmophytic communities occur on the coasts and cliffs, respectively.
The main objectives of the present study are to investigate and interpret the (a) taxonomic and phylogenetic alpha and beta-diversity patterns, (b) factors affecting island taxonomic and phylogenetic diversity and (c) the phytogeographical relationships among the studied islets and the Aegean biogeographical regions.
Materials and methods
The Northern Sporades islands and islets complex
The Northern Sporades island group (Fig. 1) belongs to the phytogeographical region of West Aegean Islands sensu Dimopoulos et al. (2013); this is a landmass that separated from the Greek mainland during the Pliocene (Dermitzakis 1990) and according to Sakellariou and Galanidou (2017) it included former peninsulas that were connected to Europe during the Last Glacial Maximum (LGM; ca 21 kyr BP) and had become isolated by sea-level rise. A shallow sea separated the Sporades ridge from the exposed land attached to north-western Anatolia (Sakellariou and Galanidou 2017). The Northern Sporades Archipelago constitutes a series of stepping stones between Central Greece and north-western Anatolia (Sakellariou and Galanidou 2017).
Northern Sporades consist of 29 islands and islets of which 22 are uninhabited. Northern Sporades were declared as the first national marine park ‘National Marine Park of Alonnisos and Northern Sporades’ of Greece in 1992 (Presidential Decree 519/28-5-92). Also, it is part of four overlapping NATURA 2000 sites, two Special Areas of Conservation (GR1430004 & GR1430003) and two Special Protection Areas (GR1430005 & GR1430009).
In the present study we focused on 23 islands and islets of the Northern Sporades (Fig. 1, Table 1). The largest island of the study area is Skopelos and the smallest one is Melissa. The highest elevation of the studied islands is the peak ‘Delfoi’ of Skopelos and has a height of 682 m. According to Bornovas and Rondogianni-Tsiambaou (1983), the study area is characterized by nine geological units (Upper Cretaceous limestones, Upper Jurassic limestones, Triassic-Jurassic formations, Flysch, Jurassic schist-chert formations, Upper Palaeozoic marine deposits, Upper Miocene marine deposits, Holocene alluvial deposits and Mio-Pliocene volcanic rocks). However, most islets are composed of limestones. Except for a part of Kyra Panagia and Piperi that is composed of marine deposits, the largest islands (Skiathos, Skopelos, Alonnisos) are composed of various geological formations. Most islets and islands are hilly with steep cliffs and many caves. All islands are of continental origin as most of the Aegean islands, apart from Psathoura and Psathonisi, which are volcanic in origin.
Only the largest islands (Skiathos, Skopelos, Alonnisos) and Peristera are inhabited. Some of the islets are seasonally or constantly grazed and none is currently cultivated, but relicts of cultivation and human interference were observed. In contrast to Skiathos, Skopelos and Alonnisos that support permanent human population, many villages and agriculture (focused on wine cultivation), grazing, cattle-raising and fishing are the main human activities in the studied N Sporades island complex. During the last years, there have been great tourist pressures as well as various other human interventions. The study area is characterized by a Mediterranean type of climate with wet winters and dry summers, belonging to the subhumid bioclimatic zone characterized by mild winters (Gouvas and Sakellarios 2011). The xerothermic index (Bagnouls and Gaussen 1953) indicates that the study area belongs to the Meso-Mediterranean zone.
Floristic data
We compiled a presence/absence island-plant matrix for the Sporades archipelago, containing 23 islands and islets and 1202 plant taxa (species and subspecies), based on (1) collections and field observations made by Panayotis Dimopoulos (UPA) and Ioannis Bazos (NKUA), who visited the islands together in late April 1999 and April and May 2000, (2) the Atlas of the Aegean Flora (Strid 2016a, b), (3) the Flora Hellenica Database (2019, unpublished data), (4) detailed floristic studies (von Halácsy 1897; Phitos 1967; Economidou 1969, 1973, 1975; Gustafsson and Snogerup 1974; Snogerup et al. 1980, 1991; Liebertz 1981; Kamari et al. 1988; Tsimburla and Yannitsaros 1992; Lowe 1999; Trigas 2003; Biel 2005; Burri and Broggi 2011; Cattaneo and Grano 2012, 2014; Cattaneo et al. 2014), (5) the Atlas of the Greek Orchids (Antonopoulos and Tsiftsis 2017; Tsiftsis and Antonopoulos 2017) and (6) Sweden’s virtual herbarium (2019+). Species nomenclature, life forms, chorological categories and habitat categories follow Dimopoulos et al. (2013, 2016) and their updates included in Dimopoulos et al. (2018). We characterized the occurrence of ‘islet specialists’ according to Bergmeier and Dimopoulos (2003) and Snogerup and Snogerup (2004). For simplicity, the term “taxa” is used hereafter for both species and subspecies (when a species has more than one subspecies) as in Dimopoulos et al. (2013, 2016).
We estimated the total number of endemics (TE) of a single island as the sum of the taxa with a distribution range restricted to Greece (i.e. Greek endemics). We did not include single island endemics in our analyses, since most of the islands and islets of the Northern Sporades island complex do not host such taxa (except for Skiathos and Piperi). The total number of plant taxa (N) and endemics (TE), as well as the geographical, climatic, and topographical data of the islands and islets of the Northern Sporades island complex are given in Table 1.
Abiotic data
We obtained current climatic data (19 bioclimatic variables) from the WorldClim database (Hijmans et al. 2005) at a 30 s resolution. Soil variables were obtained from the Soil Grids 250 m database (Hengl et al. 2017; https://www.soilgrids.org). We used seven soil variables (i.e. pH determined in KCl) that provide predicted values for the surface soil layer at varying depths (0–200 cm). Elevation data were derived from the CGIAR-CSI data-portal (http://srtm.csi.cgiar.org–Jarvis et al. 2008) and then were aggregated and resampled using functions from the ‘raster’ 2.6.7 (Hijmans 2017) package, so as to match the resolution of the other environmental variables. We also constructed 16 more climatic variables at the same resolution via the ‘envirem’ 1.1 (Title and Bemmels 2018) package based on the 19 bioclimatic variables from WorldClim. Island area was determined from 1:50,000 scale digital topographical maps obtained from the Hellenic Military Geographical Service (http://web.gys.gr/GeoSearch/). Geodiversity was compiled from the 1:500,000 scale Geological Map of Greece (Bornovas and Rondogianni-Tsiambaou 1983).
From this initial set of 45 predictors, only six were not highly correlated (Spearman rank correlation < 0.7 and VIF < 5; Online Resource 1—Rogerson 2001; Dormann et al. 2013). Multicollinearity assessment was performed with the ‘usdm’ 1.1.18 (Naimi et al. 2014) and ‘psych’ 1.8.12 (Revelle 2018) R packages. All analyses and graphs were carried out in the R 3.5.3 (R Development Core Team 2019).
Phylogenetic tree
Since molecular data are not available for many of the plant taxa occurring in the study area, in order to generate a phylogenetic tree we used the GBMB tree (Smith and Brown 2018), i.e. GenBank taxa with a backbone provided by Magallón et al. (2015) that contains 79,874 taxa. We appended any species that were present in our presence/absence matrix, but were missing from the phylogeny by adding them next to a randomly selected congener, following Bruelheide et al. (2019) and Maitner et al. (2018). We did not add the missing taxa as polytomies to their respective genera, as this approach adds substantial bias to any ensuing analyses (Davies et al. 2012). All analyses and graphs were carried out in the R 3.5.3 (R Development Core Team 2019) using functions from the ‘ape’ 5.3 (Paradis and Schliep 2018) and ‘phangorn’ 2.5.5 (Schliep 2011) R packages.
Land phylogenetic diversity
In order to quantify phylogenetic alpha diversity (PAD) and structure of island floras, we estimated PAD (sensu Faith 1992) of the species inhabiting each of the islands and islets included in the present study with the ‘picante’ 1.6-2 R package (Kembel et al. 2010). We tested for non-random patterns in PAD by estimating their standardized effect size (SES) scores as
where Xobs is the observed score within each island and mean (Xnull) and standard deviation (Xnull) are the mean and standard deviation of a null distribution of scores generated by shuffling taxa labels of the island-by-species matrix 999 times. We assessed the statistical significance of the SES scores by calculating two-tailed p values (quantiles) as
where rankobs is the rank of the observed scores compared with those of their null distributions, and runs is the number of randomizations (Kembel et al. 2010). SES scores with p < 0.05 and p > 0.95 were considered as significantly lower and higher than expected for a given PAD value, respectively. Positive SES values indicate phylogenetic overdispersion, whereas negative SES values indicate phylogenetic clustering. We calculated the standardized effect size scores with the ‘PhyloMeasures’ 2.1 (Tsirogiannis and Sandel 2017) R package. The greater sensitivity of SESPAD to more terminal structure makes it better suited to explore assembly processes working at finer temporal and spatial scales (Mazel et al. 2015).
Regression analysis of alpha diversity
In order to explore the effect of key biological, geographical and climatic variables on species richness of the Sporades archipelago, we modelled the determinants of taxonomic (N and TE) and phylogenetic (PADN and PADTE) island diversity by running a best subset regression analysis (Burnham and Anderson 2002).
A value of 1 was added to TE before log10-transformation, because zero values were reported for some islands (Brunet and Medellín 2001). Most of the variables had frequency distributions that were strongly positively skewed. Hence, the variables were log10-transformed to normalize their distribution so that they could be compared with bivariate and multivariate regression methods without heteroscedastic biases and improve the linearity of the relationships in the regression models. A goodness-of-fit test (Shapiro–Wilk and Kolmogorov–Smirnov with 95% level of confidence) to a normal distribution was used to confirm that each transformed variable was successfully transformed to an approximately normal distribution. We compared the models using R2 values as a measure of their goodness-of-fit. As the models have the same number of fitted parameters, the R2 values are directly comparable, without any modification (Triantis et al. 2003, 2005).
We used Akaike’s Information Criterion (AIC) to identify the minimum adequate models. This process also allowed calculating the relative importance of each explanatory variable, which captured the percentage of variation explained by each factor when the other factors were held constant. The variance inflator factors (VIF) were below 2.5, thus indicating that multicollinearity was not a problem in any of our obtained models. By fitting the full model, the total adjusted coefficient of multiple determination (R 2adj ) was assessed.
All analyses and graphs were carried out in the R 3.5.3 (R Development Core Team 2019) using core functions and functions from the leaps (Lumley 2009) package.
Generalized dissimilarity modelling
We used generalized dissimilarity modelling (GDM; Ferrier et al. 2007) to model pairwise plant community compositional dissimilarity between all the islands and islets included in our analysis as a response to environmental and spatial variables. Taxonomic and phylogenetic beta diversity (TBD and PBD, respectively) and their components were computed using the ‘betapart’ 1.3 package (Baselga and Orme 2012).
In the GDM framework, a group of curvilinear monotonic functions is used to regress species turnover along environmental gradients, consequently identifying the nonlinear relationship between ecological and environmental dissimilarity (Fitzpatrick et al. 2013). The significance of all variables was assessed through a Monte Carlo permutation test (999 repetitions; Manion et al. 2018). Hence, we were able to identify the most significant predictor variables. We extracted for each of these variables the fitted I-spline (a curvilinear line expressing the relationship between species turnover and each predictor—each I-spline has three coefficients). We then plotted the I-splines to investigate the magnitude and rate of beta diversity variation along the most significant predictor variables. We used the sum of the I-spline’s coefficients [it defines the proportion of compositional turnover explained by that variable and is determined by the maximum height of its I-spline (Ferrier et al. 2007; Fitzpatrick et al. 2013)] in order to quantify the magnitude of turnover along each gradient. Model fit was assessed via percent deviance explained by the model (Manion et al. 2018). The relative importance of each gradient in driving species turnover was explained as the percent change in deviance explained by the full model and the deviance explained by a model fit with that variable permuted (999 permutations; Manion et al. 2018). All GDM analyses were performed with the ‘gdm’ 1.3.7 (Manion et al. 2018) R package in the R 3.5.3 (R Core Development Team 2019).
Phytogeographical relationships
In order to investigate the phytogeographical relationships among the islands and islets constituting the Sporades archipelago, we used the TBD and PBD dissimilarity matrices of their respective turnover component, since it is least affected by variations in species richness (Baselga and Orme 2012), following the framework of Kreft and Jetz (2010). We then applied the unweighted pair-group method using the arithmetic averages (UPGMA) linkage method within a hierarchical clustering scheme, as recommended by Kreft and Jetz (2010). Finally, in order to identify distinct clusters of biogeographical affinities we used the Kelley–Gardner–Sutcliffe (KGS) penalty function, which maximizes differences between groups and cohesiveness within groups based on the respective compositional dissimilarity matrix (Kelley et al. 1996).
Sørensen’s index (Sørensen 1948): Cs = 2j/(a + b), where, j = the number of taxa common to both islets, a = the number of taxa recorded from islet 1, b = the number of taxa recorded from islet 2, was used for each pair of islets and islands, to calculate taxonomic similarity between Northern Sporades islands and islets.
Based on a presence/absence matrix of the endemic taxa occurring on the Northern Sporades island complex and the phytogeographical regions of Greece, we carried out a hierarchical cluster analysis in order to measure geographic isolation among floristic regions of Greece. Euclidean distances were computed and the Ward method (Ward 1963) was used to create groups, where the variance within the groups is minimized. The outputs were hierarchical dendrograms, using Statistica software (StatSoft Inc. 2001).
Results
Flora
The vascular flora of the Northern Sporades island complex comprises 1202 taxa (Online Resource 2), belonging to 517 genera and 120 families. Twenty-two taxa are Pteridophytes, six are Gymnosperms and 1174 are Angiosperms (Online Resource 3).
About one-third (31.6%) of the taxa found on the Northern Sporades belong to three families: Fabaceae (136 taxa), Asteraceae (122 taxa) and Poaceae (122 taxa). Also, well represented are the families Brassicaceae (50 taxa), Lamiaceae (49 taxa), Apiaceae (45 taxa), Caryophyllaceae (41 taxa) and Orchidaceae (39 taxa). The most genus-rich families of the Northern Sporades flora are Asteraceae (11.6% of all genera), followed by Poaceae (10.8% of all genera), Fabaceae and Brassicaceae (6% of all genera).
Therophytes predominate and make up 45.7% of the flora followed by hemicryptophytes and geophytes (Online Resource 3). The Mediterranean chorological category comprises 740 taxa (61.6%); within this group the circum-Mediterranean element predominates (406 taxa), while the East Mediterranean, Mediterranean-SW Asian and Mediterranean-European elements participate in almost equal proportions (8%, 7.8% and 9.4% respectively), highlighting the geographical position and climatic characteristics of the study area. The widely distributed taxa (27.7%) contribute significantly to the flora of the Northern Sporades; the Boreal, European and Circumtemperate elements are represented by few species (Online Resource 3). Balkan taxa are rather few, while the endemics, the most interesting chorological element from a phytogeographical point of view, represent 3.4% of the total flora (41 taxa).
The alien flora of the Northern Sporades comprises 41 taxa (3.4%) belonging to 33 genera and 23 families. The neophytes amount to 78% of the Northern Sporades alien flora (Arianoutsou et al. 2010; Dimopoulos et al. 2013, 2016). On the other hand, 5 alien taxa (Carpobrotus edulis, Morus nigra, Paspalum distichum, Raphanus sativus and Xanthium spinosum) occur also in small islets of the study area.
Eight islet specialists (Allium commutatum, Anthemis werneri, Atriplex recurva, Brassica cretica subsp. aegaea, Elytrigia sartorii, Malva arborea, Medicago arborea and Trigonella corniculata subsp. rechingeri—Runemark 1969; Snogerup and Snogerup 1987, 2004; Bergmeier and Dimopoulos 2003; Strid 2016a) are present in 22 of 23 islands and islets of the Northern Sporades (except of Kyra Panagia) and are found on cliffs, rocks and coastal habitats (habitat preferences as in Dimopoulos et al. 2013, 2016). More than one-third (37.5%) of the islet specialists are endemic elements and the remaining 62.5% are Mediterranean and East Mediterranean elements.
Endemism
The endemic element consists of 41 taxa making up 3.4% of its total flora, eight of them are endemic to the West Aegean islands and are found on 16 of 23 islets and islands.
The family and genus richest in endemic species are Asteraceae (10 taxa—8.2%—Online Resource 3) and Campanula (6 taxa—66.7%), respectively. The most common of them are Campanula boreosporadum, Scutellaria sporadum and Campanula scopelia. Alonnisos, Yioura (6 WAe endemics, respectively) and Kyra Panagia (5 WAe endemics) are the islands richest in WAe endemics. Moreover, two single island endemics exist in the Northern Sporades, Campanula sciathia and Campanula rechingeri found on Skiathos and Piperi, respectively.
More than two third (Online Resource 3; 73.1%) of the endemic flora are chamaephytes and hemicryptophytes and the endemism of the islets and islands ranges from 0 to 5.7% (Table 1).
Nearly one-third of the endemic taxa (36.6%) found on the Northern Sporades correspond to one or two phytogeographical areas. Seven of 33 endemic taxa (21.2%) are biregional endemics, most of them originating from the phytogeographical region of the Kiklades (Kik—Campanula reiseri, Erysimum senoneri subsp. senoneri and Fritillaria ehrhartii) and the East Aegean Islands (EAe—Centaurea rechingeri and Linum gyaricum subsp. gyaricum) followed by those originating from the phytogeographical regions of the North Aegean Islands (NAe—Malcolmia macrocalyx subsp. macrocalyx) and East-Central Greece (EC—Verbascum aphentulium).
Phylogenetic tree
A phylogenetic tree was generated for the 1202 native plant taxa based on the recently published phylogeny of seed plants by Smith and Brown (2018—Online Resource 4). Only 72 taxa (5.99%) could not be added in the GMBM tree, which was subsequently pruned, to contain only the native taxa present in the Sporades archipelago.
Island taxonomic and phylogenetic diversity
Most of the Sporades island floras show a tendency towards phylogenetic clustering (56.5% and 87% for the endemic and native plant communities, respectively; most communities were non-significantly clustered or over-dispersed; Online Resource 1). Four and five endemic and native island plant assemblages, respectively, showed significant phylogenetic clustering (SES ≤ − 1.96 and p > 0.95), while only one native island plant community (Skiathos) was significantly phylogenetically over-dispersed (SES ≥ 1.96 and p < 0.05).
Area (A), Thornthwaite aridity index (AIT) and pH were retained in the optimal model for N, while A together with AIT, annual potential evapotranspiration (PET) and precipitation seasonality (PS) were retained in the optimal model for TE (Table 2). As for both the phylogenetic diversity metrics, all six independent variables were retained in the optimal models (Table 2). Area emerged as the most important predictor for both the taxonomic diversity metrics, while climate factors and more specifically, PS and PET, were the main drivers of the endemic and native phylogenetic diversity, respectively (Table 2).
Generalized dissimilarity modelling
The GDM models explained 35.6–62.1% of deviance. (Lower values were observed for PBD than for TBD—Table 3) The most important gradient for all metrics was area, followed by pH (Figs. 2, 3, 4). Geography explained a small fraction of the deviance for all diversity metrics (Fig. 3).
The fitted functions describing the turnover rate and magnitude along each gradient were nonlinear; the turnover rate varied with position along gradients and was greatest at the high and low end of the pH and area gradient, respectively (Fig. 4). More specifically, the island plant communities differed significantly even at areas ca. 1 km2 and their composition continued to change with a rapid, but less abrupt trend until the 25 km2 threshold (Fig. 4). Soil and climate factors had a more profound effect on the endemic plant communities, which was more pronounced in TBD rather than PBD (Fig. 4).
Phytogeographical relationships
The floristic relationships between the islets studied expressed by Sørensen’s index revealed that the individual islet floras differ from each other from 15.8 to 97.1%.
The floristic independence of the islets and their floristic diversity are illustrated by the fact that 28.6% of the taxa occur on only one of the 23 studied islets and islands. None of the taxa was common to 23 islets and islands, while only 10 taxa (0.8%) were found on at least 19 islets and islands.
Among the islets and islands of the Northern Sporades, hierarchical cluster analysis based on the taxonomic diversity (Online Resource 3) and phylogenetic diversity of Greek endemic taxa (Online Resource 3) show different levels of clustering and divided them into 5 and 4 distinct groups, respectively.
Hierarchical cluster analysis (Fig. 5) revealed that West Aegean Islands (WAe) were clearly clustered with Kiklades (KiK) and East Aegean islands (EAe) and are distant from the other floristic regions based on the distribution of the Greek endemic taxa.
Discussion
Flora
Fabaceae, Asteraceae and Poaceae comprise nearly one-third of the taxa occurring in the Northern Sporades island complex and are among those best-adapted to the ecological conditions of the Mediterranean area as is confirmed by many floristic studies on Greek islands (e.g. Panitsa et al. 2003; Kougioumoutzis et al. 2012a; Iliadou et al. 2014a, b). The high percentage of therophytes (45.7%; Online Resource 3) in conjunction with the high percentage of Mediterranean (61.6%; Online Resource 3) and leguminous taxa (11.3%) reflect the Mediterranean character of the Northern Sporades flora, indicating human-induced disturbances, like grazing and cattle, in Mediterranean ecosystems (Panitsa et al. 2003; Kougioumoutzis et al. 2014b). At present, the natural ecosystems of the Northern Sporades are not endangered by the invasion of alien taxa (3.4%; Online Resource 3) which range below the percentage recorded for the entire Greek flora (c. 5% according to Arianoutsou et al. 2010; c. 3.8% according to Dimopoulos et al. 2013, 2016). The vast majority (87.8%) of the alien taxa are found only on the 3 largest, inhabited islands of Skiathos, Skopelos and Alonnisos that are most affected by human impact (tourism, grazing, fires, cultivation, etc.).
The islet specialists, known from various archipelagos around the world, occur mainly on small, low, uninhabited islets exclusively on cliffs, rocks and coastal habitats. The ‘islet phenomenon’ was first described from the Aegean, where the islet specialists constitute a characteristic phytogeographical element (Rechinger and Rechinger-Moser 1951). The islet specialists occur on 22 of the 23 islands and islets of the Northern Sporades (Online Resource 5), their distribution is restricted to the Aegean floristic regions (Runemark 1969; Snogerup and Snogerup 1987, 2004; Bergmeier and Dimopoulos 2003) and they play an important role in the floristic composition of islet vegetation (Panitsa and Tzanoudakis 2010) as a consequence of reduced competition among species, founder and isolation effects, and random eliminations by reproductive drift (Runemark 1969; Bergmeier and Dimopoulos 2003).
Endemism
The patterns of plant endemism richness we observed in Northern Sporades are similar to those occurring in Greece in general (Georghiou and Delipetrou 2010; Dimopoulos et al. 2013, 2016), at the family and genus level. Most of the endemic taxa present in the study area are chamaephytes and hemicryptophytes, scattered mainly in the numerous steep cliffs, rocks and coastal habitats, which very often harbour endemic taxa (Kypriotakis and Tzanoudakis 2001; Trigas et al. 2008; Panitsa and Kontopanou 2017).
The endemism rate of the Northern Sporades (3.4%) is relatively low compared to the total endemic flora of West Aegean islands (c. 9.5% according to Dimopoulos et al. 2013, 2016). However, the endemism rate of WAe is due to the inclusion of Evvia. Evvia is the second-richest Aegean island in terms of single island endemics (40 taxa), is characterized by three centres of endemism (the serpentine regions of N Evvia, Mt Dirphis and Mt Ochi) and hosts 175 Greek endemic elements (Trigas and Iatrou 2006; Trigas et al. 2008). Moreover, the long-lasting separation of Evvia from the Northern Sporades (Sakellariou and Galanidou 2016), in combination with its large size, mountainous landscape and environmental complexity explain why Evvia seems to be biogeographically separated from Northern Sporades, corroborated by the rather low number of WAe endemics occurring on those islands and islets. This is in line with the fact that the endemism rate grew larger the more distant islands and islets were from the adjacent mainland (Table 1); small islets generally exhibited higher endemism rates than the larger islands.
The only single island endemic of Skiathos is the schizoendemic and obligate chasmophyte Campanula sciathia that belongs to the section Quinqueloculares (Boiss.) Phitos. The section’s taxa and their distributional patterns (Campanula cymaea near Kymi, Campanula constantinii on Mt Dirphis and Campanula scopelia found on Skopelos and Kyra Panagia) have emerged as a result of the region’s palaeogeographical history (Phitos 1965; Eddie and Ingrouille 1999; Crowl et al. 2015).
Island taxonomic and phylogenetic diversity
Island diversity patterns
Area emerged as the most important variable shaping taxonomic plant diversity (Table 2), while niche-based processes played a lesser role in driving these patterns. This phenomenon is also observed in other Aegean island complexes and biogeographical regions, where niche-based processes play a lesser role in shaping the geographical distribution and species richness patterns of the Aegean island plant communities (Panitsa et al. 2010; Kagiampaki et al. 2011; Kallimanis et al. 2011; Kougioumoutzis and Tiniakou 2014; Kougioumoutzis et al. 2017). On the other hand, island phylogenetic diversity is predominantly determined by climate, implying that the region’s spatial configuration plays a minor role in shaping these diversity patterns, a phenomenon also observed in oceanic archipelagos as well (Cabral et al. 2014). This concurs with the presence of niche conservatism in Mediterranean ecosystems (Rundel et al. 2016; Skeels and Cardillo 2017) and with the fact that most endemics occurring in the Aegean seem to use the same ecological niche (Kallimanis et al. 2011).
Most island and islet floras showed a general tendency for phylogenetic clustering. Nevertheless, Skiathos was the sole exception to this rule, suggesting that the relative importance of different processes shaping species composition varies among the Northern Sporades islands. More specifically, phylogenetic clustering could be due to either increased island isolation, in situ speciation or strong environmental filtering (e.g. Webb et al. 2002; Weigelt et al. 2015). On the other hand, phylogenetic overdispersion could arise from competitive exclusion of closely related species or environmental filtering across multiple habitats on a given island (e.g. Cavender-Bares et al. 2009). The phylogenetic overdispersion observed in Skiathos, the largest island in our study system and the one closer to the adjacent mainland, could be due to reduced environmental filtering, since larger islands tend to be topographically and environmentally more complex and thus provide more opportunities for the establishment and long-term survival of different clades with different niche requirements (Parent and Crespi 2006; Cardillo et al. 2008). Skiathos’ flora may have also been enriched with migrating lineages from adjacent mainland regions, due to its longer-lasting connection with Evvia and Sterea Ellas during Pleistocene glacial periods. Putative refugia tend to be phylogenetically over-dispersed (e.g. Kooyman et al. 2011, 2013), a phenomenon recently highlighted for some forest communities in mainland Greece (Mastrogianni et al. 2019).
Beta-diversity patterns and affecting factors
The Mediterranean region’s intricate geography and orography (Rundel et al. 2016 and references therein) have resulted in high taxonomic beta diversity (Thompson 2005), a phenomenon apparent in our study area as well, since most Northern Sporades islands and islets have very few endemic species in common (floristic differences range: 15.8–97.1%). The palaeogeographical history of Northern Sporades and the subsequent sea-level and island area changes may have resulted in reduced species dispersal, thus promoting isolation and high beta diversity among the island plant communities, which could be the result of differential allopatric speciation and extinction (e.g. Campanula sciathia, C. scopelia, C. rechingeri and other taxa belonging to the section Quinqueloculares (Boiss.) Phitos). Moreover, the responses of endemic PBD were consistently smaller than those of TBD. This implies that phylogenetically close relatives have probably been responsible for the spatial variation in Northern Sporades plant diversity.
Beta-diversity patterns at a global scale are better predicted by topographical heterogeneity as expressed by elevation rather than biotic transitions (e.g. Peixoto et al. 2017), while in the Mediterranean, these patterns seem to be driven by climatic factors as well (e.g. Molina-Venegas et al. 2017; Xystrakis et al. 2019). This concurs with our results, since PBD and TBD patterns are mainly shaped by area and several environmental gradients, such as PET, AIT and PWM (Figs. 2, 3, 4). As in the Ionian archipelago (Valli et al. 2019), geographical distance only plays a minor role in establishing the beta-diversity patterns (Figs. 2, 3, 4), pointing out that dispersal filtering is not the dominant process regarding the assembly of the Northern Sporades island flora. This is in line with previous works stating that climate has also contributed to the biogeographical configuration of the Aegean archipelago (Kougioumoutzis et al. 2014a, 2017; Kougioumoutzis and Tiniakou 2014). This is also in harmony with the fact that nearly 40% of the present Aegean flora (excluding the endemics) has reached the Aegean Islands owing to human action in prehistoric or early historic times (Greuter 1979).
The presence of these human-dispersed species may explain the lesser part dispersal limitation has in shaping the PBD patterns of the Northern Sporades island complex.
Phytogeographical relationships
Our results on phytogeographical relations are based on the analysis of the Greek endemic taxa for two reasons: (1) when we ran the analysis based on the total number of taxa (i.e. the native taxa), the results were similar, yet the trends not so obvious as those obtained from the Greek endemics and more importantly, (2) when dealing with phytogeographical issues in the Aegean, only these taxa, rather than the whole flora, are phytogeographically meaningful and informative (Greuter 1975).
Most of the island and islet pairs showed rather high floristic dissimilarity, with the notable exception of Yioura and Kyra Panagia. The flora of these islands is very similar and is probably the result of their long-lasting isolation from the other Northern Sporades islands and islets (Kamari et al. 1988; Snogerup 1991; Snogerup et al. 1991). The vast majority of taxa occurring on Yioura and Kyra Panagia are relicts of different age which may have survived on these two islands due to the long-lasting isolation that may have hindered the arrival and establishment of competitors (Snogerup 1991). The high floristic similarity between Yioura and Kyra Panagia is in harmony with the land connection between these two islands since the late Messinian, c. 4.5 Mya (Kamari et al. 1988—Online Resource 3).
Hierarchical cluster analyses based on the taxonomic and phylogenetic diversity of Greek endemics (Online Resource 3) divided the Northern Sporades into distinct groups clearly based on area and elevation of the islets and islands, as these factors according to Kallimanis et al. (2011) and Kougioumoutzis et al. (2017), are the major drivers of plant diversity patterns in the Aegean area.
West Aegean islands (WAe) were grouped with Kiklades (Kik) and East Aegean islands (EAe) based on the distribution of Greek endemic taxa. On the other hand, Evvia which is phytogeographically very well separated from the Northern Sporades clusters together with Sterea Ellas and Peloponnisos.
According to Georghiou and Delipetrou (2010), the Northern Sporades show higher phytogeographical affinities to the adjacent mainland regions of Sterea Ellas, East-Central Greece and the Peloponnese (StE, EC and Pe, respectively) and then with the phytogeographical region of the Kiklades (Kik). According to our results, seven taxa are biregional endemics [i.e. taxa useful for inferring phytogeographical relationships in the Aegean (Kougioumoutzis et al. 2012a, 2012b, 2014b, 2015)] and three of them—namely Campanula reiseri, Erysimum senoneri subsp. senoneri and Fritillaria ehrhartii—are exclusively found in WAe and Kik, two occur exclusively in WAe and EAe (Centaurea rechingeri and Linum gyaricum subsp. gyaricum), while only one occurs either in NAe or EC.
Therefore, we argue that the Northern Sporades seem to be closer connected to Kik and EAe rather than to StE, EC and Pe as previously suggested for the entire WAe phytogeographical region (Georghiou and Delipetrou 2010), which was probably heavily influenced by the inclusion of Evvia—an entity entirely different from the Northern Sporades island complex in biogeographical terms (Kougioumoutzis et al. 2017).
Our results thus lend weight to the hypothesis that the Northern Sporades (along with Skyros) seem to be closer connected to the Kiklades rather than to Evvia or the adjacent mainland, due to their longer isolation and separate palaeogeographical history during the Quaternary (Sakellariou and Galanidou 2017; Simaiakis et al. 2017). These results are also in accordance with the findings of Kougioumoutzis et al. (2017) that two main distributional and (palaeo-) geographical barriers exist in the Aegean Archipelago: the Mid-Aegean trench and the North Aegean Trough that limit the phytogeographical connections between the Northern Sporades with Kriti and North Aegean islands, respectively.
References
Antonopoulos Z, Tsiftsis S (2017) Atlas of the Greek orchids, vol 2. Mediterraneo Editions, Rethymno
Arianoutsou M, Delipetrou P, Celesti-Grapow L et al (2010) Comparing naturalized alien plants and recipient habitats across an east-west gradient in the Mediterranean Basin. J Biogeogr 37:1811–1823. https://doi.org/10.1111/j.1365-2699.2010.02324.x
Bagnouls F, Gaussen H (1953) Saison séche et indice xérothermique. Doc Cart Prod Vég 3:1–47
Baselga A, Orme CDL (2012) betapart: an R package for the study of beta diversity. Meth Ecol Evol 3:808–812. https://doi.org/10.1111/j.2041-210X.2012.00224.x
Bergmeier E, Dimopoulos P (2003) The vegetation of islets in the Aegean and the relation between the occurrence of islet specialists, island size, and grazing. Phytocoenologia 33:447–474. https://doi.org/10.1127/0340-269X/2003/0033-0447
Biel B (2005) Contributions to the flora of the Aegean islands of Santorini and Anafi (Kiklades, Greece). Willdenowia 35:87–96. https://doi.org/10.3372/wi.35.35106
Bornovas I, Rondogianni-Tsiambaou T (1983) Geological Map of Greece. Scale 1:500,000, 2nd edn. Institute of Geology and Mineral Exploration, Division of General Geology and Economic Geology, Athens
Bruelheide H et al (2019) sPlot—a new tool for global vegetation analyses. J Veg Sci 30:161–186. https://doi.org/10.1111/jvs.12710
Brunet AK, Medellín RA (2001) The species–area relationship in bat assemblages of tropical caves. J Mammal 82:1114–1122. https://doi.org/10.1644/1545-1542(2001)082%3c1114:tsarib%3e2.0.co;2
Burnham KP, Anderson DR (2002) Model selection and multimodel inference: a practical–theoretical approach, 2nd edn. Springer, New York
Burri C, Broggi M (2011) Zur Orchideenflora der Insel Alonnisos (Nordsporaden, Griechenland). J Eur Orch 43:378–400
Cabral JS, Weigelt P, Kissling WD, Kreft H (2014) Bio-geographic, climatic and spatial drivers differentially affect alpha, beta and gamma diversities on oceanic archipelagos. Proc Roy Soc B Biol Sci 281:20133246
Cardillo M, Gittleman JL, Purvis A (2008) Global patterns in the phylogenetic structure of island mammal assemblages. Sci Proc Roy Soc B Biol 275:1549–1556. https://doi.org/10.1098/rspb.2008.0262
Cattaneo C, Grano M (2012) Indagine preliminare sulla flora dell’isola egea di Alonissos (Sporadi settentrionali, Grecia). Boll Soc Nat “Silvia Zenari” 35:81–100
Cattaneo C, Grano M (2014) Note sul paesaggio vegetale e sulla flora vascolare estivia dell’isola egea di Skiathos e delle sue isole circonvicine Tsougriá e Aspróniso (Sporadi settentrionali, Grecia). Ann Mus Civ Rovereto 29:243–288
Cattaneo C, Grano M, Pasta S (2014) Note sul paessagio naturale e sulla flora vascolare di Skopelos (Sporadi settentrionali, Grecia). Nat Sicil Ser IV 38:3–42
Cavender-Bares J, Kozak KH, Fine PVA, Kembel SW (2009) The merging of community ecology and phylogenetic biology. Ecol Lett 12:693–715. https://doi.org/10.1111/j.1461-0248.2009.01314.x
Critical Ecosystem Partnership Fund (2017). Mediterranean basin biodiversity hotspot. Ecosystem profile. Prepared by Birdlife International
Crowl AA, Visger CJ, Mansion G et al (2015) Evolution and biogeography of the endemic Roucela complex (Campanulaceae: Campanula) in the Eastern Mediterranean. Ecol Evol 5:5329–5343. https://doi.org/10.1002/ece3.1791
Darwin C (1859) On the origin of species by means of natural selection, or the preservation of favoured races in the struggle for life. John Murray, London
Davies TJ, Kraft NJ, Salamin N, Wolkovich EM (2012) Incompletely resolved phylogenetic trees inflate estimates of phylogenetic conservatism. Ecology 93:242–247. https://doi.org/10.1890/11-1360.1
Denslow JS (2001) The ecology of insular biotas. Trends Ecol Evol 16:423–424. https://doi.org/10.1016/S0169-5347(01)02153-X
Dermitzakis DM (1990) Paleogeography, geodynamic processes and event stratigraphy during the Late Cenozoic of the Aegean area. In: International symposium on biogeographical aspects of insularity, Roma 1987. 85. Accademia Nazionale dei Lincei, pp 263–288
Dimopoulos P, Raus T, Bergmeier E, et al (2013) Vascular plants of Greece: an annotated checklist. Englera, 31. Botanischer Garten und Botanisches Museum Berlin-Dahlem, Freie Universitat Berlin and Hellenic Botanical Society, Berlin and Athens. https://doi.org/10.3372/wi.46.46303
Dimopoulos P, Raus T, Bergmeier E et al (2016) Vascular plants of Greece: an annotated checklist. Supplement. Willdenowia 46:301–347. https://doi.org/10.3372/wi.46.46303
Dimopoulos P, Raus T, Strid A (ed.) (2018) Flora of Greece web. Vascular plants of Greece. An annotated checklist. Version II (June 2018). Available at: http://portal.cybertaxonomy.org/flora-greece/. Accessed 20 Aug 2018
Dormann CF, Elith J, Bacher S et al (2013) Collinearity: a review of methods to deal with it and a simulation study evaluating their performance. Ecography 36:27–46. https://doi.org/10.1111/j.1600-0587.2012.07348.x
Economidou E (1969) Geovotaniki erevna nisou Skiathou. Fitogeografia ton Vorion Sporadon. PhD Thesis, University of Athens, Athens (In Greek)
Economidou E (1973) Contribution à l’étude de la flore et de la phytogéographie de l’île de Skopelos. Phytogéogr Sporades Nord II. Biol Gall 5:77–121
Economidou E (1975) La végétation des Îles des Skiathos et Skopelos (Sporades du Nord). Comparaisons gé obotaniques. Veröff Geobot Inst ETH Zürich 55: 198–237
Eddie WMM, Ingrouille MJ (1999) Polymorphism in the Aegean “five-loculed” species of the genus Campanula, Section Quinqueloculares (Campanulaceae). Nordic J Bot 19:153–169. https://doi.org/10.1111/j.1756-1051.1999.tb00659.x
Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biol Conserv 61:1–10. https://doi.org/10.1016/0006-3207(92)91201-3
Ferrier S, Manion G, Elith J, Richardson K (2007) Using generalized dissimilarity modelling to analyse and predict patterns of beta diversity in regional biodiversity assessment. Diversity Distrib 13:252–264. https://doi.org/10.1111/j.1472-4642.2007.00341.x
Fitzpatrick M, Sanders N, Normand S et al (2013) Environmental and historical imprints on beta diversity: insights from variation in rates of species turnover along gradients. Proc Roy Soc B Sci 280:20131201
Georghiou K, Delipetrou P (2010) Patterns and traits of the endemic plants of Greece. Bot J Linn Soc 162:130–422. https://doi.org/10.1111/j.1095-8339.2010.01025.x
Gouvas M, Sakellarios N (2011) Climate and forest vegetation of Greece. National Observatory of Athens, Athens
Graham CH, Fine PVA (2008) Phylogenetic beta diversity: linking ecological and evolutionary processes across space in time. Ecol Lett 11:1265–1277. https://doi.org/10.1111/j.1461-0248.2008.01256.x
Graham CH, Parra JL, Rahbek C, McGuire JA (2009) Phylogenetic structure in tropical hummingbird communities. Proc Natl Acad Sci 106:19673–19678. https://doi.org/10.1073/pnas.0901649106
Greuter W (1975) Historical biogeography of the southern half of the Aegean area. Problems of Balkan flora and vegetation. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp 17–21
Greuter W (1979) The origins and evolution of Island Floras as exemplified by the Aegean Archipelago. In: Bramwell D (ed) Plants and Islands. Academic Press, Cambridge, pp 87–106
Greuter W (1995) Origin and peculiarities of Mediterranean island floras. Ecol Médit 21:1–10
Gustafsson M, Snogerup S (1974) Studies in the Aegean flora. XXII. The flora of the island of Skantzoura. Bot Not 127:364–372
Hengl T, de Jesus JM, Heuvelink GB et al (2017) SoilGrids250m: global gridded soil information based on machine learning. PLoS ONE 12:e0169748. https://doi.org/10.1371/journal.pone.0169748
Hijmans RJ (2017) raster: geographic data analysis and modeling
Hijmans RJ, Cameron SE, Parra JL et al (2005) Very high resolution interpolated climate surfaces for global land areas. Int J Climatol 25:1965–1978. https://doi.org/10.1002/joc.1276
Iliadou E, Kallimanis AS, Dimopoulos P, Panitsa M (2014a) Comparing the two Greek archipelagos plant species diversity and endemism patterns highlight the importance of isolation and precipitation as biodiversity drivers. J Biol Res 21:1–10. https://doi.org/10.1186/2241-5793-21-16
Iliadou E, Panitsa M, Raus T, Dimopoulos P (2014b) Flora and factors affecting species diversity in protected “Natura 2000” sites of the Ionian area: the Echinades islet group (Greece). Willdenowia 44:121–136. https://doi.org/10.3372/wi.44.44113
Jarvis A, Reuter HI, Nelson A, Guevara E (2008) Hole-filled SRTM for the globe: version 4. CGIAR Consortium for Spatial Information. Available at: http://srtm.csi.cgiar.org/
Kagiampaki A, Triantis K, Vardinoyannis K, Mylonas M (2011) Factors affecting plant species richness and endemism in the South Aegean (Greece). J Biol Res 16:282–295
Kallimanis AS, Bergmeier E, Panitsa M et al (2010) Biogeographical determinants for total and endemic species richness in a continental archipelago. Biodivers Conserv 19:1225–1235. https://doi.org/10.1007/s10531-009-9748-6
Kallimanis AS, Panitsa M, Bergmeier E, Dimopoulos P (2011) Examining the relationship between total species richness and single island palaeo- and neo-endemics. Acta Oecol 37:65–70. https://doi.org/10.1016/j.actao.2010.11.008
Kamari G, Phitos D, Snogerup B, Snogerup S (1988) Flora and vegetation of Yioura, N Sporades, Greece. Willdenowia 17:59–85
Kelley LA, Gardner SP, Sutcliffe MJ (1996) An automated approach for clustering an ensemble of NMR derived protein structures into conformationally related subfamilies. Protein Engin Design Selec 9:1063–1065. https://doi.org/10.1093/protein/9.11.1063
Kembel SW, Cowan PD, Helmus MR et al (2010) Picante: R tools for integrating phylogenies and ecology. Bioinformatics 26:1463–1464. https://doi.org/10.1093/bioinformatics/btq166
Kooyman R, Rossetto M, Cornwell W, Westoby M (2011) Phylogenetic tests of community assembly across regional to continental scales in tropical and subtropical rain forests. Global Ecol Biogeogr 20:707–716. https://doi.org/10.1111/j.1466-8238.2010.00641.x
Kooyman RM, Rossetto M, Sauquet H, Laffan SW (2013) Landscape patterns in rainforest phylogenetic signal: Isolated islands of refugia or structured continental distributions. PLoS ONE. https://doi.org/10.1371/journal.pone.0080685
Kougioumoutzis K, Tiniakou A (2014) Ecological factors driving plant species diversity in the South Aegean Volcanic Arc and other central Aegean islands. Pl Ecol Diversity 8:173–186. https://doi.org/10.1080/17550874.2013.866989
Kougioumoutzis K, Tiniakou A, Georgiadis T, Georgiou O (2012a) Contribution to the flora of the South Aegean Volcanic Arc: The Methana Peninsula (Saronic Gulf, Greece). Edinburgh J Bot 69:53–81. https://doi.org/10.1017/S0960428611000394
Kougioumoutzis K, Tiniakou A, Georgiou O, Georgiadis T (2012b) Contribution to the flora of the South Aegean Volcanic Arc: Anafi Island (Kiklades, Greece). Willdenowia 42:127–141. https://doi.org/10.3372/wi42.42115
Kougioumoutzis K, Simaiakis SM, Tiniakou A (2014a) Network biogeographical analysis of the central Aegean archipelago. J Biogeogr 41:1848–1858. https://doi.org/10.1111/jbi.12342
Kougioumoutzis K, Tiniakou A, Georgiou O, Georgiadis T (2014b) Contribution to the flora of the South Aegean volcanic arc: Kimolos Island (Kiklades, Greece). Edinburgh J Bot 71:135–160. https://doi.org/10.1017/S0960428614000055
Kougioumoutzis K, Tiniakou A, Georgiou O, Georgiadis T (2015) Contribution to the flora and biogeography of the kiklades: Folegandros Island (Kiklades, Greece). Edinburgh J Bot 72:391–412. https://doi.org/10.1017/S0960428615000128
Kougioumoutzis K, Valli AT, Georgopoulou E et al (2017) Network biogeography of a complex island system: the Aegean Archipelago revisited. J Biogeogr 44:651–660. https://doi.org/10.1111/jbi.12920
Kraft NJB, Comita LS, Chase JM et al (2011) Disentangling the drivers of β diversity along latitudinal and elevational gradients. Science 333:1755–1758. https://doi.org/10.1126/science.1208584
Kreft H, Jetz W (2010) A framework for delineating biogeographical regions based on species distributions. J Biogeogr 37:2029–2053. https://doi.org/10.1111/j.1365-2699.2010.02375.x
Kueffer C, Fernández-Palacios JM (2010) Comparative ecological research on oceanic islands. Perspect Pl Ecol Evol Syst 12:81–82. https://doi.org/10.1016/j.ppees.2010.02.001
Kypriotakis Z, Tzanoudakis D (2001) Contribution to the study of the Greek insular flora: the chasmophytic flora of Crete. Bocconea 13:495–503
Liebertz J (1981) Die Orchideen der Insel Skiáthos. Orchid 32:59
Lowe MR (1999) Notes sur les orchidées des Sporades du Nord (Grèce). Nat Belges 80:155–172
Lumley T (2009) Using Fortran code by Alan Miller, leaps: regression subset selection. R package, Version 2.3. Available at: https://CRAN.R-project.org/package=leaps. Accessed Aug 2017
Magallón S, Gómez-Acevedo S, Sánchez-Reyes L, Hernández-Hernández T (2015) A metacalibrated time-tree documents the early rise of flowering plant phylogenetic diversity. New Phytol 207:437–453. https://doi.org/10.1111/nph.13264
Maitner BS, Boyle B, Casler N et al (2018) The bien r package: a tool to access the Botanical Information and Ecology Network (BIEN) database. Meth Ecol Evol 9:373–379. https://doi.org/10.1111/2041-210X.12861
Manion G, Lisk M, Ferrier S, et al (2018) gdm: generalized dissimilarity modeling. R package ver. 1.3.8. Available at: https://cran.r547project.org/package=gdm
Mastrogianni A, Kallimanis AS, Chytrý M, Tsiripidis I (2019) Phylogenetic diversity patterns in forests of a putative refugial area in Greece: a community level analysis. Forest Ecol Managem 446:226–237. https://doi.org/10.1016/j.foreco.2019.05.044
Mazel F, Renaud J, Guilhaumon F, Mouillot D, Gravel D, Thuiller W (2015) Mammalian phylogenetic diversity–area relationships at a continental scale. Ecol 96:2814–2822
Mazel F, Wüest RO, Lessard JP, Renaud J, Ficetola GF, Lavergne S, Thuiller W (2017) Global patterns of β-diversity along the phylogenetic time-scale: the role of climate and plate tectonics. Global Ecol Biogeogr 26:1211–1221. https://doi.org/10.1111/geb.12632
Médail F (2017) The specific vulnerability of plant biodiversity and vegetation on Mediterranean islands in the face of global change. Regional Environm Change 17:1775–1790. https://doi.org/10.1007/s10113-017-1123-7
Médail F, Myers N (2004) Mediterranean basin. In: Mittermeier RA et al (eds) Hotspots revisited. Earth’s biologically richest and most endangered terrestrial ecoregions. CEMEX, Mexico City, pp 144–147
Médail F, Quezel P (1997) Hot-spots analysis for conservation of plant biodiversity in the Mediterranean Basin. Ann Missouri Bot Gard. https://doi.org/10.2307/2399957
Molina-Venegas R, Aparicio A, Lavergne S, Arroyo J (2017) Climatic and topographical correlates of plant palaeo- and neoendemism in a Mediterranean biodiversity hotspot. Ann Bot 119:229–238. https://doi.org/10.1093/aob/mcw093
Myers N, Mittermeier RA, Mittermeier CG et al (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858. https://doi.org/10.1038/35002501
Naimi B, Hamm NAS, Groen TA et al (2014) Where is positional uncertainty a problem for species distribution modelling? Ecography 37:191–203. https://doi.org/10.1111/j.1600-0587.2013.00205.x
Panitsa M, Kontopanou A (2017) Diversity of chasmophytes in the vascular flora of Greece: floristic analysis and phytogeographical patterns. Bot Serb 41:199–211. https://doi.org/10.5281/zenodo.1026483
Panitsa M, Tzanoudakis D (2010) Floristic diversity on small islands and islets: Leros islets’ group (east Aegean area, Greece). Phytol Balcan 16:271–284
Panitsa M, Snogerup B, Snogerup S, Tzanoudakis D (2003) Floristic investigation of Lemnos island (NE Aegean area, Greece). Willdenowia 33:79–105. https://doi.org/10.3372/wi.33.33108
Panitsa M, Tzanoudakis D, Triantis KA, Sfenthourakis S (2006) Patterns of species richness on very small islands: the plants of the Aegean archipelago. J Biogeogr 33:1223–1234. https://doi.org/10.1111/j.1365-2699.2006.01481.x
Panitsa M, Tzanoudakis D, Sfenthourakis S (2008) Turnover of plants on small islets of the eastern Aegean Sea within two decades. J Biogeogr 35:1049–1061. https://doi.org/10.1111/j.1365-2699.2007.01846.x
Panitsa M, Trigas P, Iatrou G, Sfenthourakis S (2010) Factors affecting plant species richness and endemism on land-bridge islands—an example from the East Aegean archipelago. Acta Oecol 36:431–437. https://doi.org/10.1016/j.actao.2010.04.004
Panitsa M, Kagiampaki A, Kougioumoutzis K (2018) Plant diversity and biogeography of the Aegean Archipelago: A new synthesis. In: Sfenthourakis S, Pafilis P, Parmakelis A, Poulakakis N, Triantis K (eds) Biogeography and biodiversity of the Aegean. In honour of Prof. Moysis Mylonas. Broken Hill Publisher, Lefkosa
Paradis E, Schliep K (2018) ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R. Bioinformatics 35:526–528
Parent CE, Crespi BJ (2006) Sequential colonization and diversification of Galapagos endemic land snail genus Bulimulus (Gastropoda, Stylommatophora). Evolution (Lancaster) 60:2311–2328. https://doi.org/10.1554/06-366.1
Peixoto FP, Villalobos F, Melo AS et al (2017) Geographical patterns of phylogenetic beta-diversity components in terrestrial mammals. Global Ecol Biogeogr 26:573–583. https://doi.org/10.1111/geb.12561
Phitos D (1965) Die quinquelokulären Campanula-Arten. Österr Bot Z 112:449–498
Phitos D (1967) Florula Sporadum. Phyton 12:102–149
R Development Core Team (2019) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available at: http://www.r-project.org
Rechinger KH, Rechinger-Moser F (1951) Phytogeographia Aegaea. Denkschr Akad Wiss Wien, Math-Naturwiss Kl 105:1–208
Revelle W (2018) psych: procedures for personality and psychological research, version 1.8.12. Northwestern University, Evanston, Illinois. Available at: https://CRAN.R-project.org/package=psych
Rogerson PA (2001) Statistical methods for geography. SAGE Publications, Ltd, London. https://doi.org/10.4135/9781849209953
Rundel PW, Arroyo MTK, Cowling RM et al (2016) Mediterranean biomes: evolution of their vegetation, floras, and climate. Annual Rev Ecol Evol Syst 47:383–407. https://doi.org/10.1146/annurev-ecolsys-121415-032330
Runemark H (1969) Reproductive drift, a neglected principle in reproductive biology. Bot Not 122:90–129
Sakellariou D, Galanidou N (2016) Pleistocene submerged landscapes and Palaeolithic archaeology in the tectonically active Aegean region. Geol Soc London, Spec Publ 411:145–178. https://doi.org/10.1144/SP411.9
Sakellariou D, Galanidou N (2017) Aegean pleistocene landscapes above and below sea-level: palaeogeographic reconstruction and hominin dispersals. In: Bailey GN, Harff J, Sakellariou D (eds) Under the sea: archaeology and palaeolandscapes of the continental shelf. Springer, Berlin, pp 335–336
Schliep KP (2011) phangorn: phylogenetic analysis in R. Bioinformatics 27:592–593
Sfenthourakis S, Panitsa M (2012) From plots to islands: species diversity at different scales. J Biogeogr 39:750–759. https://doi.org/10.1111/j.1365-2699.2011.02639.x
Siefert A, Ravenscroft C, Weiser MD, Swenson NG (2013) Functional beta-diversity patterns reveal deterministic community assembly processes in eastern North American trees. Global Ecol Biogeogr 22:682–691. https://doi.org/10.1111/geb.12030
Simaiakis SM, Rijsdijk KF, Koene EFM et al (2017) Geographic changes in the Aegean Sea since the Last Glacial Maximum: postulating biogeographic effects of sea-level rise on islands. Palaeogeogr Palaeoclimatol Palaeoecol 471:108–119. https://doi.org/10.1016/j.palaeo.2017.02.002
Skeels A, Cardillo M (2017) Environmental niche conservatism explains the accumulation of species richness in Mediterranean-hotspot plant genera. Evolution (Lancaster) 71:582–594. https://doi.org/10.1111/evo.13179
Smith SA, Brown JW (2018) Constructing a broadly inclusive seed plant phylogeny. Amer J Bot 105:302–314. https://doi.org/10.1002/ajb2.1019
Snogerup S (1991) Endemism and disjunct populations on Yioura and Kira Panagia—an urgent case for conservation. Bot Chron 10:293–297
Snogerup S, Snogerup B (1987) Repeated floristical observations on islets in the Aegean. Pl Syst Evol 155:143–164. https://doi.org/10.1007/BF00936296
Snogerup S, Snogerup B (2004) Changes in the flora of some Aegean islets 1968–2000. Pl Syst Evol 245:169–213. https://doi.org/10.1007/s00606-003-0100-4
Snogerup S, von Bothmer R, Gustafsson M (1980) Floristic report from the island of Psathura (Greece). Bot Not 133:145148
Snogerup S, Snogerup B, Phitos D et al (1991) Flora and vegetation of Kira Panagia, N Sporades, Greece. Bot Chron 10:547–566
Sørensen T (1948) A method of establishing groups of equal amplitude in plant sociology based on similarity of species content. Biol Skr Kongel Danske Vidensk Selsk 5:1–34
StatSoft Inc. (2001) STATISTICA (data analysis software system), version 6. Available at: http://www.statsoft.com
Strid A (1996) Phytogeographia Aegaea and the flora Hellenica database. Ann Naturhist Mus Wien 98:279–289
Strid A (2016a) Atlas of the Aegean flora. Part 1: text & plates. Englera 33:1–700
Strid A (2016b) Atlas of the Aegean flora. Part 2: maps. Englera 33:1–878
Swenson NG (2013) The assembly of tropical tree communities—the advances and shortcomings of phylogenetic and functional trait analyses. Ecography 36:264–276. https://doi.org/10.1111/j.1600-0587.2012.00121.x
Thompson JD (2005) Plant evolution in the Mediterranean. Oxford University Press, Oxford
Title PO, Bemmels JB (2018) ENVIREM: an expanded set of bioclimatic and topographic variables increases flexibility and improves performance of ecological niche modeling. Ecography 41:291–307. https://doi.org/10.1111/ecog.02880
Triantis KA, Mylonas M (2009) Greek islands, biology. In: Gillespie R, Glague DA (eds) Encyclopedia of islands. University of California Press Ltd, Berkeley
Triantis KA, Mylonas M, Lika K, Vardinoyannis K (2003) A model for the species-area-habitat relationship. J Biogeogr 30:19–27. https://doi.org/10.1046/j.1365-2699.2003.00805.x
Triantis KA, Mylonas M, Weiser MDD et al (2005) Species richness, environmental heterogeneity and area: a case study based on land snails in Skyros archipelago (Aegean Sea, Greece). J Biogeogr 32:1727–1735. https://doi.org/10.1111/j.1365-2699.2005.01322.x
Trigas P (2003) Contribution to the study of the endemism of the flora of the island of Evvia (W Aegean, Greece). PhD Thesis, University of Patras, Patras
Trigas P, Iatrou G (2006) The local endemic flora of Evvia (W Aegean, Greece). Willdenowia 36:257–270. https://doi.org/10.3372/wi.36.36121
Trigas P, Iatrou G, Panitsa M (2008) Vascular plant species diversity, biogeography and vulnerability in the Aegean islands as exemplified by Evvia island (W Aegean, Greece). Fresenius Environm Bull 17:48–57
Tsiftsis S, Antonopoulos Z (2017) Atlas of the Greek orchids, vol. 1. Mediterraneo Editions, Rethymno
Tsimburla E, Yannitsaros A (1992) A preliminary study of the flora and vegetation of the island Piperi. In: Proceedings 12th annual meeting Hellenic society for biological sciences, Mitilini 27–29.4. 1990, Athine, pp 172–173 (In Greek)
Tsirogiannis C, Sandel B (2017) PhyloMeasures: fast and exact algorithms for computing phylogenetic biodiversity measures. R package version 2.1. Available at: https://CRAN.R-project.org/package=PhyloMeasures
Valli A-T, Kougioumoutzis K, Iliadou E et al (2019) Determinants of alpha and beta vascular plant diversity in Mediterranean island systems: the Ionian islands. Greece. Nordic J Bot 37:e02156. https://doi.org/10.1111/njb.02156
Vogiatzakis IN, Mannion AM, Sarris D (2016) Mediterranean island biodiversity and climate change: the last 10,000 years and the future. Biodivers Conservation 25:2597–2627. https://doi.org/10.1007/s10531-016-1204-9
von Halácsy E (1897) Florula Sporadum. Oesterr Bot Z 47:92–99
Ward JH (1963) Hierarchical grouping to optimize an objective function. J Amer Stat Assoc 58:236–244
Webb CO, Ackerly DD, McPeek MA, Donoghue MJ (2002) Phylogenies and community ecology. Annual Rev Ecol Syst 33:475–505. https://doi.org/10.1146/annurev.ecolsys.33.010802.150448
Weigelt P, Daniel Kissling W, Kisel Y et al (2015) Global patterns and drivers of phylogenetic structure in island floras. Sci Rep 5:12213. https://doi.org/10.1038/srep12213
Weinstein BG, Tinoco B, Parra JL et al (2014) Taxonomic, phylogenetic, and trait beta diversity in South American Hummingbirds. Amer Nat 184:211–224. https://doi.org/10.1086/676991
Whittaker RJ, Fernández-Palacios JM (2007) Island biogeography: ecology, evolution, and conservation. Oxford University Press, Oxford
Xystrakis F, Mitsios-Antonakos D, Eleftheriadou E et al (2019) Inter-regional beta-diversity patterns of the woody flora of Greece. Ann Forest Res 62:33–50. https://doi.org/10.15287/afr.2018.1077
Acknowledgments
The Hellenic Ministry of the Environment and Energy is gratefully acknowledged for funding Panayotis Dimopoulos and Ioannis Bazos while surveying the vegetation of the Northern Sporades islands and islets complex during the years 1999 and 2000, in the framework of the project on habitat types mapping of the Natura 2000 sites in Greece.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Handling editor: Ivana Resetnik.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Contribution to “Plants of the Balkan Peninsula in Space and Time”.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Information on Electronic Supplementary Material
Information on Electronic Supplementary Material
Online Resource 1. Abbreviations and correlation matrix of all the abiotic variables included in the analyses. Standardized effect scores of the phylogenetic alpha diversity for each island and islet included in the analyses. Dissimilarity matrix based on Sørensen’s index.
Online Resource 2. List of species occurring in the Northern Sporades islands and islets complex.
Online Resource 3.a Numbers of vascular plant taxa in the flora of the Northern Sporades islet complex. b Life forms in the total (1202 taxa) and the endemic (41 taxa) native flora of the Northern Sporades islet complex. c Chorological spectrum of the native flora of the Northern Sporades island complex. d Families with Greek-, WAe- and Single-Island endemics and degree of endemism. e Hierarchical cluster analysis showing floristic relations between the Northern Sporades islet complex based on the phylogenetic diversity (PBD) of Greek endemic taxa. f Hierarchical cluster analysis showing floristic relations between the Northern Sporades islet complex based on the taxonomic diversity (TBD) of Greek endemic taxa.
Online Resource 4. The phylogenetic tree of the species included in our analyses based on the GBMB phylogenetic tree published by Smith & Brown (2018).
Online Resource 5. Plant species of special biogeographical interest.
Rights and permissions
About this article
Cite this article
Iliadou, E., Bazos, I., Kougioumoutzis, K. et al. Taxonomic and phylogenetic diversity patterns in the Northern Sporades islets complex (West Aegean, Greece). Plant Syst Evol 306, 28 (2020). https://doi.org/10.1007/s00606-020-01660-0
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s00606-020-01660-0