Abstract
Pyropia yezoensis (red algae) or commonly known as nori, is highly regarded for its nutritional benefits and distinct taste, leading to its widespread consumption. The bio-activity and sensory characteristics of P. yezoensis are attributed to the metabolites it contains. In this study, identification and quantification of the diverse range of metabolites of P. yezoensis and metabolomic analysis were conducted using gas chromatography-mass spectrometry (GC-MS). Furthermore, the impact of high temperature on its metabolites regulation was also investigated. Due to metabolomic analysis, a diverse range of metabolites were identified in P. yezoensis, including lipids, amino acids, carbohydrates, and secondary metabolites. Several known bioactive compounds, including alcohol and polyols, amines, amino acids-peptides-analogues, beta hydroxy acids and derivatives, carbohydrates and carbohydrate conjugates, cholestane steroids, dicarboxylic acid and derivatives, and fatty acids and conjugates were detected in abundance, highlighting the nutritional and functional properties of P. yezoensis. Additionally, the metabolites composition of P. yezoensis was significantly affected in high temperatures, which led to up-regulation of considerable primary metabolites and few were down-regulated, and suggested a potential response and adaptation mechanism of P. yezoensis to elevated temperature conditions. This research highlighted the metabolomics of P. yezoensis, provided insights into its metabolite composition and regulatory responses to high temperature conditions, enhanced our knowledge of the biochemical pathways and adaptive mechanisms of P. yezoensis, which can assist the improvement strategies of utilization and cultivation to promote this valuable alga in response to fluctuating environmental conditions.
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Data Availability Statement
The data that support the findings of this study are openly available from the corresponding author upon reasonable request.
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The authors acknowledge with gratitude, the efforts and contributions of Shanghai OE Biotech. Co., Ltd., China
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Supported by the National Key R&D Program of China (No. 2016YFC1402102), the National Natural Science Foundation of China (No. 41976109), the Ministry of Natural Resources Key Laboratory of Eco-Environmental Science and Technology of China (No. MEEST-2020-2), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Zeb, A., Yang, X., Khan, Y. et al. Non-targeted metabolomic analysis of Pyropia yezoensis metabolites using GC-MS and its regulation under high temperature. J. Ocean. Limnol. (2024). https://doi.org/10.1007/s00343-024-4081-1
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DOI: https://doi.org/10.1007/s00343-024-4081-1