Abstract
Coral mucus secreted by mucocytes provides a protective physicochemical and physiological barrier between coral tissue and external environmental threats. The biomolecules and nutrients of the secreted mucus are derived from endosymbionts, coral polyps and support coral functions, such as feeding, sediment clearing and protection, against numerous biotic and abiotic stressors. The surface mucus layer also houses a diverse community of beneficial microorganisms that defend against pathogens. Enzymes including peptidases, esterases, and glycosidases were observed and described in mucus. Most importantly, the presence of phenoloxidase, an intracellular enzyme in secretory mucus, generally triggers melanin synthesis, providing fast-acting components of invertebrate immunity in disease resistance. However, the purpose and the mechanism of mucus release, effects of mucus components on defense properties, and functional roles in intra- and interspecific interactions are not well described. Thus, the present review aims to understand the mucus components and the complex roles played by mucus in microbial associations, feeding, and resilience that influence coral health.
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Introduction
According to recent studies, 20% of coral reefs have already been irreparably damaged, and additionally 24% of the remaining reefs are facing serious threats due to anthropogenic stresses, such as overfishing and eutrophication (Sharma and Ravindran 2020; Galli et al. 2021). Biotic and abiotic stressor can cause coral bleaching, a process in which the algal symbionts are expelled from the host coral (Hafezi et al. 2020; Ravindran et al. 2022). The absence of the endosymbiont will weaken coral health, and mucus secretion will be affected (Ravindran et al. 2022). Environmental impacts, including temperature rise, on the secretion of mucus have not been studied in detail (Vaughan et al. 2021). For example, mucus release is enhanced during high sedimentation (Reynaud and Ferrier-Pages 2019), and when corals are exposed to air at low tide (Wild et al. 2004, 2005). In addition, mucus aggregations in the water column increase sedimentation and nutrient recycling (Wild et al. 2005; Plass-Johnson et al. 2015), which control microbial activities (Bythell and Wild 2011). Therefore, a better understanding of the changes in mucus caused by biological and physical stressors may help us to monitor coral health under different environmental conditions.
Mucus is a slimy substance produced by epithelial cells called mucocytes utilizing the organic carbon synthesized by photosynthetic endosymbiotic algae (Brown and Bythell 2005; Bakshani et al. 2018). Mucus production varies between and within coral species (Coffroth 1990; Hadaidi et al. 2019). It is mainly composed of proteolytic enzymes, microbes, mucins, proteins, polysaccharides, lipids and their substructures, such as monosaccharides, amino acids and mycosporines (Brown and Bythell 2005; Stabili 2019; Vilas Bhagwat et al. 2023). The beneficial microbes within the mucus form a barrier against potential coral pathogens, competing for nutrients and space or producing antibiotics (Ritchie 2006; Krediet et al. 2013; Raina et al. 2016; Irudayarajan et al. 2023). Coral mucus has several important functions, including protection against pathogens and UV radiation, capturing food, acting as an energy carrier, producing antibiotics, and serving as a nutrient source for coral-associated microbial communities (Bakshani et al. 2018; Bythell and Wild 2011; Vilas Bhagwat et al. 2023). Coral mucus contains up to 1000 times more bacteria than seawater (Nakajima et al. 2015). Vibrio shiloi, the causative agent of bleaching in the Mediterranean coral, and Oculina patagonica bind to the receptors of the surface mucus layer during temperature rise (Thompson et al. 2014). However, it has been demonstrated that beneficial probiotic bacteria enable O. patagonica to become resistant to V. shiloi infection (Reshef et al. 2006). Thus, studying the components of coral mucus is of great importance for a better understanding of the entire mucosal system, its interactions with other microorganisms (commensals and pathogens), and their involvement in coral immunity and health. Further, although the composition of coral mucus has been described in some studies (Coddeville et al. 2011; Hadaidi et al. 2019), it is limited to a few coral species and the sources of mucus components in mucus are not clearly understood. Therefore, the present study aims to describe the significant role of coral mucus in maintaining the health and functionality of coral reef ecosystems, especially the synthesis and constituents of coral mucus and their critical roles in protecting coral health.
Coral mucus synthesis and secretion
The mucocyte cells of the coral polyp synthesize mucus, with the major component being carbon-based carbohydrates, and are then secreted out as a coral surface layer (Brown and Bythell 2005). The coral-associated endosymbiotic algae are believed to provide the maximum carbon required for mucus synthesis although the source of the entire carbon requirement is not clear (Crossland 1987; Brown and Bythell 2005; Fang 2015) (Fig. 1). The other compounds required for mucus, such as amino acids, fatty acids, lipids, glycerol and nicotinamide adenine dinucleotide phosphate (NADPH), come from the coral diet (Hadaidi 2018). Only mucocytes contribute to mucus synthesis in corals, unlike in humans where multiple cell types are involved (Hadaidi et al. 2019; McShane et al. 2021). Some other gland cells are observed in coral tissues, but their roles are still unclear (Bruckner 2015). However, in vertebrates, the transformation of similar gland cells to mucus cells has been observed, which may be because these cells are either specialized secretory cells or developing mucocytes (Que 2015). The distribution of mucocytes varies in both abundance and location, not just within individual colonies but also between coral species (Baker et al. 2022). The production of coral mucus can be triggered by various factors, such as changes in light intensity, water flow, and the presence of predators or food particles (Zetsche et al. 2016). For example, an increase in the sea surface temperature increases the mucocyte density in host tissue; however, it decreases after bleaching (Piggot et al. 2009). In addition, more organic components are found in mucus from stressed than in healthy corals (Lee et al. 2016).
The synthesis of coral mucus is unique because it is derived from a significant portion of the photosynthetically fixed carbon (Hadaidi et al. 2019; Brown and Bythell 2005), and contains a high concentration of arabinose and xylose, which is not typically found in animal cells. Different coral species have varied rates of mucus production (Crossland et al. 1980; Kurihara et al. 2018), depending on the endosymbiont contribution of energy-rich glycerol, alanine, and glucose to the host (Burriesci et al. 2012; Gabay 2018) (Fig. 1). Excess photosynthetic carbon is eliminated by the coral through mucus release (Bakshani et al. 2018) along with lipids, such as wax esters and triglycerides for mucus synthesis (Krueger et al. 2018). Acropora spp. release up to 4.8 L of mucus from a 1m2 patch in one day and 56–80% of the mucus is dissolved in water (Wild et al. 2004), of which 20–40% of mucus forms strings and filaments (Stabili 2019). This mucus net filters seawater up to 100 ml cm2 of tissue per hour (Lewis 1976). Subsequently, heterotrophic coral reef organisms utilize the energy from the materials trapped in the coral mucus and the energy derived from zooxanthellae (Wild et al. 2004). Invertebrate mucus protects, lubricates, and helps in locomotion, mating, homing, and foraging, performing much more diverse functions than in vertebrates (Denny 1989). In addition, mucus released on the epithelial surface aims to attract particulate matter.
Mucus composition
Mucins and the importance of mucus rheology
The glycoprotein core, mucin, is made up of 20% polymers and 80% sugars and contains cysteine, which undergoes cleavage during mucus dispersion (Bakshani et al. 2018). O-glycosylation of mucin occurs on oligosaccharides in the core between serine and threonine and on the N-acetylgalactosamine carbohydrate chains, and N-glycosylation occurs on asparagine (Corfield 2015). The side chains of mucins are either branched or linear and up to 20 monosaccharides long and are linked with either β1 → 2 or β1 → 3 bonds (Krediet et al. 2009). The structural diversity of mucin is due to its carbohydrate composition (Thornton and Sheehan 2004). The ability of mucin to form a gel affects mucus function depending on its extensive glycosylation and polymerization ability (Younan et al. 1982). The von Willebrand D (VWD) domain is involved in the polymerization of mucin monomers via intermolecular disulfide bonds (Ambort et al. 2012). The outer layer of the epidermis of fishes continuously produces new goblet cells because they cannot produce mucus more than once. But coral mucus goblet cells can continuously produce mucus from the same cells (Subramanian et al. 2007).
Corals require relatively fluid mucus for their ciliary feeding and to prevent the desiccation of coral polyps by air exposure during low tide (Brown and Bythell 2005; Bessell-Browne et al. 2017). Based on rheology, coral mucus is distributed as fluid mucus, string, web or flocs, and mucus sheets (Bessell-Browne et al. 2017). Distinct mucin characteristics are present across various layers within the coral surface mucus layer (SML) (Guppy et al. 2019). The mucin concentration and gel-forming properties of mucus are necessary for its protective and lubricating functions (Thornton and Sheehan 2004). Human mucus becomes hydrated and mucus contents turn it into a gel (Piludu et al. 2003). As polymeric mucins have been detected in coral mucus, similar to vertebrates (Jatkar et al. 2010a), it is possible that coral mucus also contains different types of mucin molecules that convert it from gel to liquid and liquid to gel as needed (Horricks et al. 2019). Because coral mucus is rich in plant-associated sugar arabinose, it may have some carbohydrate chains that differ from those of mammalian mucins (Wild et al. 2005). Human mucus changes its rheology from viscoelastic gel to viscous fluid at high stress and returns to a viscous gel at low stress (Jatkar et al. 2010b) making it a lubricant and helping it move under various physiological conditions (Hill et al. 2022). A similar property may also be present in the coral SML, which remains to be investigated. However, corals can produce two types of mucus. The milked mucus has no gel-forming properties but can be converted to a gel by a higher concentration of mucin (Jatkar et al. 2010a), which is very similar to human respiratory mucus (Zetsche et al. 2016). Mucus sheets are sticky mucus layers derived from liquid mucus (Bessell-Browne et al. 2017). These are then transferred into the food web of the reef (Brown and Bythell 2005).
Carbohydrates
The relative concentration of carbohydrates varies among coral species, yet their composition remains constant, with certain sugars being shared across multiple species, and this variability is intricately linked to host phylogeny, microbial associations, and symbiont composition (Hadaidi et al. 2019). The mucosal sugars glucose, mannose, xylose, and galactose are found in all coral species that have been analyzed, whereas arabinose, N-acetylglucosamine, ribose, fucose, and rhamnose are present in only a few species (Table 1). Thermal stress has been shown to induce changes in both the enrichment and abundance of various carbohydrate pools, with a notable impact on galactose levels (Hillyer et al. 2017). For example, a significant decrease in glucose, galactose, and mannose was described under rising temperature-stress conditions (Lee et al. 2016). Thus, mannose, glucose, and galactose can serve as markers for temperature stress, while N-acetyl glucosamine exhibits anti-inflammatory properties and reduces the production of free radicals (Salvatore et al. 2000). N-acetyl galactosamine also helps in the O-glycosylation of coral mucin molecules. Xylose and arabinose are types of plant sugars commonly utilized as energy sources, indicating that they may have come from the endosymbiont (Teplitski et al. 2016). Arabinose is widespread in Acropora spp., suggesting that it is transferred from algal symbionts to the host (Wild et al. 2005, 2010; Lee et al. 2016).
The carbohydrate composition of mucus differs among the corals Acropora pharaonis, Porites verrucosa, and Porites lobata (Hadaidi et al. 2019). However, Stylophora pistillata and Pocillopora verrucosa, two pocilloporid corals, have similar carbohydrate (Neave et al. 2017) and zooxanthellae composition (LaJeunesse et al. 2018). Differences in mucus components could affect microbial community composition in coral reef habitats (Schottner et al. 2009). The most common mucus components observed in corals that occur across a large geographic range (e.g., A. muricata, Pachyseris speciosa, Fungia fungites, Stylophora sp., and Pocillopora sp.) are fucose, arabinose, glucose, galactose, the amino sugar N-acetylglucosamine, xylose, and mannose (Wild et al. 2005; Lee et al. 2016) (Table 1). Thus, mucus carbohydrates are essential substrates that support bacterial growth and contribute to the flux of dissolved organic matter (DOM) in seawater (Riemann and Azam 2002).
Fatty acids of mucus
The precise understanding of the fatty acid (FA) composition and its role in coral mucus is still inadequate and remains one of the least studied aspects of coral mucus. It was reported that the FA contents of Acropora and Porites species were almost the same, but the proportions were different not only in the mucus but also in comparison with the tissues (Ravindran et al. 2020). The most common and abundant FAs observed in the mucus of Acropora sp. in Discovery Bay, Jamaica, were palmitic acid and palmitoleic acid (Means and Sigleo 1986; Wild et al. 2005). These were also found more frequently in corals and zooxanthellae as structural and storage lipids (Patton et al. 1977; Means and Sigleo 1986). Other FAs, such as myristic acid and linoleic acid, have also been observed in mucus, but in lower amounts (Wild et al. 2005). The most abundant saturated FAs in marine organisms are palmitic acid (C16:0), palmitoleic acid (C16:1), and myristic acid (C14:0), similar to those found in certain unicellular marine algae (Uslu et al. 2021). Even though the presence of FAs in mucus has been reported, their origins have not been documented.
FAs in coral mucus are essential for the health and functioning of coral reefs, providing energy, supporting microbial communities, and aiding in defense mechanisms (Bachok et al. 2006). Polyunsaturated fatty acids (PUFAs), especially n−3 and n−6 PUFAs, regulate metabolism, including growth, respiration, energy generation, energy reserves, membrane constituents, photosynthesis, planula larvae and egg production, biomass formation and general enzyme activity, survival, and stress resistance (Bachok et al. 2006). C20:5n−3 FAs are essential for sperm maturation and C20:4n−6 is essential for egg maturation, and saturated (SAFA) and monounsaturated FAs (MUFA) are stored as triglycerides and wax esters (Grottoli et al. 2004; Tchernov et al. 2004; Treignier et al. 2008). In all of the previously studied coral mucus samples, higher concentrations of SAFAs were observed (Fig. 2). In addition, FAs in corals serve as markers for bacterial colonization and diversity, coral health, and food source tracking (Volkman et al. 1980). As the synthesis of FAs in corals and in coral mucus is directly coupled to photosynthesis, there is a significant correlation between dinoflagellate biomarkers and FA concentrations (Oku et al. 2003). Free fatty acids (FFAs) have been found to be involved in maintaining cell membrane stability and cell signaling (Binienda et al. 2013). FFAs are physiologically active as dietary components and participate in cell signaling (Zbigniew et al. 2013). FAs are also required for reproduction and membrane fluidity (Lin et al. 2012). However, the purpose and the function of FAs present in mucus are not well understood and need to be thoroughly investigated in various genera and species of corals based on their habitat and other environmental parameters.
Mucus lipids
Photosynthetic carbon is stored in the cytoplasm of zooxanthellae, in the form of lipids, which are then incorporated into the coral mucus, where it serves as an important source of energy for a variety of marine organisms (Crossland et al. 1980; Pasaribu et al. 2015). Many galactolipids synthesized by marine diatoms have been shown to induce apoptosis starting at micromolar concentrations in mammalian cells (Andrianasolo et al. 2008), suggesting the function of membrane lipids in regulating the symbiosis between corals and zooxanthellae. Coral mucus is composed of high-energy lipids which are also an important source of energy for fish (Wright et al. 2019). Upon thermal stress, corals may acquire more thermotolerant symbionts (Lajeunesse et al. 2010) having lipids with more SAFAs with a high melting point. This altered membrane lipid composition is observed in bleached corals, indicating differences in heat sensitivity among zooxanthellae lineages (Tchernov et al. 2004).
Zooxanthellae provides precursors and metabolites required for endogenous lipid synthesis in coral hosts mainly through photosynthesis (Oku et al. 2003; Iluz and Dubinsky 2015), and several studies have demonstrated the rapid conversion of photosynthetically fixed carbon to lipids (Radice et al. 2019). The presence of lipids (e.g., wax esters) in coral mucus has been demonstrated in significant amounts (Crossland 2021). Corals are rich in lipids, which are the biochemical link between the symbiotic mutualistic association of coral and zooxanthellae (Sikorskaya et al. 2022). Lipids are involved in coral physiological and biochemical processes and are an important source of long-term stored energy as mucus serves as an energy-rich source of nutrition for various organisms (Rodrigues et al. 2008). During bleaching, a change in FA composition and also a reduction in the rate of lipid production may affect the coral’s metabolic needs (Grottoli et al. 2004). This reduced energy reserve increases disease susceptibility and mortality (Smith et al. 2020), which may be due to a change in the microbial community in the damaged tissues.
Proteins/peptides of mucus
According to Benavides et al. (2017), a low C:N ratio in coral mucus indicates the presence of a large amount of protein. Previous studies on Palythoa sp. and Fungia sp. showed that their mucus contains 8–20% organic matter and 1–2% protein (Daumas and Thomassin 1977; Stabili et al. 2015). Coral mucus from Acropora, Fungia, and Sarcophyton species contains important enzymes, such as peptidases, esterases, and glycosidases (Vacelet and Thomassin 1991; Vilas Bhagwat et al. 2023). Hydroxyproline, found in coral mucus, is crucial for collagen synthesis and maintaining the thermodynamic stability of collagen's triple-helical conformation and associated tissues (Ortega et al. 2018). Fish mucus, on the other hand, is known for its high protein content and enzymes, such as lysozyme, alkaline phosphatase, complement, cathepsin B, transferrin, and C-reactive protein (Subramanian et al. 2007; Zhao et al. 2008). The mucus of the zoanthid Palythoa caribaeorum contains lectins and proteases (Guarnieri et al. 2018). However, phenoloxidase (PO), peroxidases (POD), and antioxidant enzymes like superoxide dismutase, catalase, and glutathione peroxidase, which have been reported from coral tissues (Anithajothi et al. 2014), have not yet been reported in coral mucus. Thus, mucus reported with antimicrobial properties has to be investigated for its potential peptides and proteins.
Amino acids
Corals and zooxanthellae can synthesize 20 different amino acids, with some produced by the coral polyps, others by the zooxanthellae, and yet others by both partners. However, different coral species have different amino acid synthesis abilities (Fitzgerald and Szmant 1997). Amino acids can also be taken up via plankton, detritus, and the water column. The acquired amino acids are used by the coral to synthesize proteins for tissue growth, calcification, and other processes (Grover et al. 2008; Shinzato et al. 2014). The production of methionine could be a result of the synergistic action of enzymes from both coral and zooxanthellae, indicating that these symbiotic partners have evolved to mutually benefit each other (Shinzato et al. 2014). Although corals acquire many amino acids, only 16 have been observed in the mucus, including aspartate, glutamine, serine, glycine, threonine, arginine, alanine, tyrosine, valine, phenylalanine, isoleucine, leucine, lysine, histidine and methionine, of several coral species (i.e., Acropora formosa, Pachyseris speciosa, Fungia fungites, Sarcophyton sp., Lemnalia sp., Cespitularia sp., Platygyra lamellina, Heteroxenia fuscesens, Oculina arbuscula (Meikle et al. 1988; Ducklow and Mitchell 1979; Means and Sigleo 1986; Coddeville et al. 2011).
Analysis of mucus produced by Palythoa and Fungia species has shown that the amino acids serine, aspartic acid, glutamic acid, and glycine are present in high abundance, and these play important roles in a variety of biological processes, including protein synthesis, cellular signaling, and energy metabolism (Daumas and Thomassin 1977). Glycine is the most abundant amino acid found in both coral tissues and mucus, particularly in species such as Montastraea cavernosa (Daumas and Thomassin 1977). Glutamic acid, which also acts as an antioxidant in many plants, animals, and bacteria, was more abundant in the mucus of Fungia sp., Acropora sp., Platygyra sp., Sarcophyton sp., and Heteroxenia sp., compared to serine, and methionine (Ducklow and Mitchell 1979). Alanine is generally observed in Porites divaricata, Acropora cervicornis, Orbicella faveolata, and the azooxanthellate corals, Astrangia poculata and Tubastraea coccinea (Fitzgerald and Szmant 1997). Similarly, Fungia scutaria was reported to utilize tyrosine, lysine, glycine and aspartic acid, from surrounding water (Stephens 1962). This suggests that corals acquire some amino acids through heterotrophic feeding from the surrounding water. In addition, both host and zooxanthellae can synthesize amino acids and this varies from species to species.
Mucus antioxidants against oxidative stress
A decrease in zooxanthellae density is due to oxidative stress causing coral bleaching (Downs et al. 2002; Weis 2008). Primarily, oxidative stress is generated during photosynthesis (Krieger-Liszkay 2005). Excessive production of reactive oxygen species (ROS) causes disorganization and rupture of the thylakoid membrane (Tchernov et al. 2004). Free radicals, such as hydrogen peroxide (H2O2) and hydroxyl radicals (–OH), degrade mucus glycoproteins at the histidine molecule, so that mucus property, molecular weight, and viscosity is affected (Dailah 2022). ROS imparts an adverse impact on the mucus defense properties which are then controlled by the mucus antioxidants (Suggett et al. 2008).
The presence of phenoloxidase (PO), an enzyme that activates the invertebrate defense system, was reported in coral secretory mucus (Rivera-Ortega and Thome 2018; Vilas Bhagwat et al. 2023). The mechanism of secretion of this enzyme into coral mucus is not known. However, the expulsion of similar molecules from tissue to the mucus was reported previously (Ritchie 2006). Thus, phenoloxidase is thought to be an intracellular enzyme, but similar activity was also observed in the mucus of other organisms, such as in lancelets (Zhang and Li 2000) and some insect cuticles (Arakane et al. 2016). The pro-phenoloxidase (proPO) activates PO, resulting in the synthesis of melanin which in turn activates innate immune responses. The proPO system itself is activated by tyrosine and phenylalanine, which is synthesized by both the coral host as well as zooxanthellae (Fitzgerald and Szmant 1997). To reduce the impact of toxic by-products, such as reactive oxygen (RO) and other free radicals, antioxidant enzymes are up-regulated to offset self-harm (Gardner et al. 2016). However, the source and the presence of such antioxidant enzymes and non-enzymatic antioxidants in coral mucus are not well studied (Vilas Bhagwat et al. 2023). Some enzymes, such as superoxide dismutase and glutathione peroxidase, have been reported as stress markers in corals (Gardner et al. 2016). Elevated PO activity triggers melanin production, serving as a signal for corals to react to pathogens, and concurrently functioning as a potent antioxidant against UV radiation (Palmer et al. 2011). The antioxidant capabilities of mucus against oxidative stress may determine the survival of the corals against bleaching and other stressors.
Antimicrobial activity of mucus and mucus-associated microbes
Coral mucus from different habitats is composed of unique components that alter the presence of specific microbes on the surface of different coral species (Guppy and Bythell 2006; McShane et al. 2021). The natural flora of healthy coral is presumably beneficial and any shift in this bacterial population may be an early indicator of coral stress, such as disease (Nithyanand et al. 2011). Therefore, the SML of coral is a selective medium determined by host–microbe–environment interactions, where the commensal microbiota act against potential pathogens (Ritchie 2006; Alagely et al. 2011; Krediet et al. 2013; Teplitski et al 2016; Irudayarajan et al. 2023). The microbial gene abundance in the coral mucus is known to be involved in stress resistance, virulence, chemotaxis and motility, utilization of FAs and lipids, sulfur and nitrogen metabolism, and secondary metabolism making the mucus-associated bacterium a first line of defense (Bythell and Wild 2011; Irudayarajan et al. 2023). Thus, some microbial communities in the SML are described as producing defensive antimicrobial chemicals (Orland and Kushmaro 2009; Irudayarajan et al. 2023). The Caribbean coral Acropora palmata demonstrated with antibacterial inhibition against the pathogen that causes white pox disease (Nissimov et al. 2009). Similarly, antibacterial activity was found in many cultivable bacteria of the coral mucus of six scleractinian corals from the Gulf of Eilat (Orland and Kushmaro 2009). Varied bacterial genera of both tissue and mucus of A. digitifera, including Vibrio sp. and Bacillus sp. have been elucidated for their antibacterial activity. Bacillus sp. synthesizes a significant peptide group of antibiotics including gramicidin, bacitracin and polymyxin B (Wiese et al. 2009). Thus, the highly specific mucus bacterial community is crucial for first line of defense of corals against diseases and other environmental stress factors (Kemp et al. 2015). Moreover, specific bacteriophages of the coral SML have been described to treat various bacterial infections as phage therapy (Nguyen et al. 2015; van Oppen 2019). The SML antimicrobial activity exhibited species specificity, while the mucus-associated beneficial bacteria, such as Pseudoalteromonas sp. and Roseobacter sp., have been described for their antibacterial activity against a number of coral pathogens, including V. shiloi (Ortega and Thome 2018). As a result, beneficial microorganisms of corals (BMCs) are used as probiotic drugs in order to increase coral resistance to environmental stress, and the rotifers, Brachionus plicatilis, are employed as carriers to impart BMCs into the coral Pocillopora damicornis (Assis et al. 2020).
The biochemicals present in the surface mucus of Acropora palmata and Pseudodiploria strigosa are responsible for regulating the coral-associated microbial community (Rivera-Ortega et al. 2018). Studies have shown that the mucus of A. palmata suppresses the growth of various bacterial strains, including Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium, and the white pox disease causative agent, Serratia marcescens (Ritchie 2006; Vilas Bhagwat et al. 2023). The mucus also exhibits antibacterial activity against both Gram-positive and Gram-negative bacteria (Ritchie 2006; Vilas Bhagwat et al. 2023). Similarly, healthy coral mucus obtained from P. strigosa has been found to possess antimicrobial activity against Serratia marcescens and Aurantimonas sp. pathogens (Ortega and Thome 2018). In addition to its antimicrobial properties, Galaxea fascicularis mucus has been found to have potential health benefits for humans, such as apoptotic and anticancer activity, DNase activity, and activity against Topoisomerase I and II enzymes (Ding et al. 1999; Khalesi 2015). Homarine, an organic compound with antibiotic potential, has been documented in corals for its ability to potentially inhibit the growth of a range of bacteria and fouling diatoms (Maldonado et al. 2016). Fish mucus contains a variety of components, including disulfides, glycoconjugates, antimicrobial peptides (AMPs), lysozyme, cathepsin B, complement, alkaline phosphatase, C-reactive protein, transferrin, and others, which act as effective protection (Meyer et al. 2007; Subramanian et al. 2007; Zhao et al. 2008). AMPs in fish mucus are effective antimicrobial agents against human pathogens, including algae, bacteria, fungi, viruses, or parasites (Komatsu et al. 2009; Fernandes et al. 2010). Although the antimicrobial properties of coral mucus and associated bacteria have been mentioned in numerous papers, the specific components responsible for these properties are not well documented (Wright et al. 2019; Wijayanti et al. 2020), and further research is needed to better understand the immunological properties of coral mucus and the mechanisms by which it provides protection.
Mucus as a sunscreen and effective antioxidant under heat stress
Mycosporine-like amino acids (MAAs) are responsible for shielding corals from the harmful effects of solar ultraviolet (UV) radiation by absorbing approximately 10% of UV radiation. This mechanism is critical for the survival of corals, as exposure to high levels of UV radiation can cause damage to coral DNA, resulting in a range of negative impacts on coral health and the overall health of the surrounding ecosystem. By providing this important protection against UV radiation, MAAs play a key role in maintaining the health and resilience of coral populations (Teai et al. 1998; Kageyama and Waditee-Sirisattha 2019). Mycosporine-glycine, mycosporine-2glycine, palythine, mycosporine-methylamine-serine, and mycosporine-methylamine-threonine, which were characterized from Lobophyllia, Acropora, Fungia, and Pocillopora species, have all been reported from coral mucus (Teai et al. 1998). In addition to their ability to combat oxidative stress, MAAs can protect many marine organisms from the harmful effects of light (Shick and Dunlap 2002). Similarly, mycosporine-glycine showed an additional antioxidant property in coral mucus along with sunscreen, probably protecting endosymbionts from oxidative damage produced through photosynthesis (Teai et al. 1998). Biologically harmful UV rays between 310 and 360 nm are absorbed by MAAs. Endosymbionts and the coral hosts are both protected from the harmful effects of UV radiation by mucus MAAs. It was reported that the UV-absorbing molecules in the coral mucus of Fungia fungites reduced with depth (Drollet et al. 1997). The reduction in UV radiation, light, and photosynthesis may be attributed to increased depth, as water absorbs and scatters UV radiation more effectively with increasing depth, leading to a decrease in UV radiation intensity.
Mycosporine-like amino acid concentrations vary depending on coral species and habitat. They have been found in both host tissues as well as in zooxanthellae (Shick and Dunlap 2002). MAAs are thermally stable, making them useful for scavenging reactive oxygen species under heat stress (Banaszak and Lesser 2009). MAAs play a role in cellular osmoregulation during salinity stress or in a high-salinity environment (Oren and Gunde-Cimerman 2007). The combination of mycosporine and glycine, the most common MAAs in coral hosts, protects the coral symbiosis from oxidative damage (Banaszak et al. 2006). MAA also serves as a nitrogen source within the cell, which is required for growth and proliferation (Oren and Gunde-Cimerman 2007).
Mucus as a source of nutrients for marine organisms
Mucus produced by coral is a valuable energy source with high glucose content. As glucose is readily available, it is a highly preferred energy source for microbes and other organisms (Ravindran et al. 2023). Thus, coral mucus is an important component of marine ecosystems, providing a rich source of energy for a wide group of organisms (Wild et al. 2010) (Table 2). Mucus energy levels are far greater than benthic photosynthetic algae. Further, a few soft corals have also been shown to feed on the mucus of other corals (Coffroth 1984). Similarly, fishes, such as blue sprat (Eckes et al. 2015), damselfishes (Coles and Strathmann 1973; McMohan et al. 2016), and other benthic organisms, such as an Acoelomorpha worm (Waminoa sp.), bivalves (Shafir and Loya 1983), shrimp (Coralliocaris superba) (Horka et al. 2016), coral crabs (Trapezia sp.) (Shmuel et al. 2022) and some planktonic organisms, such as crown-of-thorns starfish larvae (Nakajima et al. 2016), copepods (Acartia negligence) (Richman 1975), and mysids, (Mysidium integrum) (Gottfried and Roman 1983) also exhibit feeding on coral mucus. Coral mucus was found to be the best nutritional source with an C:N ratio of mucus fluid of 6.9 to 13.7 and mucus sheet of 4.8 to 5.9 (Coffroth 1990) (Table 2). Since mucus sheets that stay on the coral surface can trap organic materials, their nutritional value is higher than reef benthic algae (~ 1) and reef algal detritus (~ 1) (Wilson et al. 2003). When compared with the other forms of energy sources, reef fish feces contribute to a nutritional value of ~ 10 (Bailey and Robertson 1982), phytoplankton ~ 3.5 (Renaud et al. 1999), and zooplankton ~ 5.5 (Ventura 2006). Furthermore, coral mucus acts as a vehicle for nutrients to reach the mouth (Goldberg 2018). Small reef fish and copepods utilize wax esters and triglycerides as energy sources, both of which are major components of mucus (Ho 2017). Thus, coral mucus helps in the fulfillment of energy requirements as well as the cycling of nutrients (Wild et al. 2004).
Role of mucus in reef ecosystem nutrient cycling
When coral mucus is released into the water column, it is rapidly colonized by microorganisms that break down the organic compounds and release nutrients, such as nitrogen (N2) and phosphorus (Van Oppen et al. 2019). These nutrients are then available for uptake by other organisms in the ecosystem, such as phytoplankton and zooplankton. Corals obtain N2 through predation and from the water column and excrete ammonia (Fig. 3) (Siboni et al. 2008). Further, Archaea use this ammonia, which plays an important role in ocean nitrification. During daytime, when oxygen levels are high due to photosynthesis, certain types of Archaea convert ammonia into nitrite. Conversely, at night, when oxygen is low, different types of Archaea carry out denitrification to convert nitrite into nitrogen (Siboni et al. 2008, 2012). Nitrogen-cycling microbes play a vital role in coral–algae symbiosis, especially under nutrient-rich or -poor conditions for coral productivity (Radecker et al. 2015). Coral mucus contains high concentrations of dissolved organic nitrogen (DON), which can be readily taken up by bacteria for growth and metabolism. Some of these bacteria form symbiotic relationships with coral polyps, where they fix atmospheric nitrogen (N2) (Diaz 2017). Other bacteria in the mucus help us to recycle nitrogen within the holobiont by breaking down waste products and releasing ammonium (NH4+) and nitrate (NO3−) into the surrounding water. These forms of nitrogen can then be taken up by other organisms in the reef ecosystem (Wild et al. 2004).
Up to 80% of mucus released by corals becomes dissolved organic carbon (DOC) (Wild et al. 2004). Sponges transfer dissolved organic matter (DOM) including DOC and DON to the next trophic level (Rix et al. 2017). Bacteria utilize mucus and break down organic compounds and release carbon dioxide (CO2) through respiration. This CO2 can then be taken up by photosynthetic organisms, such as algae and seagrasses, which use it to produce organic carbon through photosynthesis (Haas et al. 2016). This process helps us to maintain the balance of carbon in the reef ecosystem. Coral mucus also contains significant amounts of dissolved organic phosphorus (DOP), which can be taken up by bacteria and recycled within the holobiont (Nakajima et al. 2015). Some of these bacteria are capable of breaking down complex organic phosphorus compounds, such as phospholipids, to release inorganic phosphorus (PO43−) into the surrounding water (Bourne et al. 2013). This inorganic phosphorus can then be taken up by algae and other organisms in the reef ecosystem. Mucus functions as nutrient trapping, thus, higher ammonia, nitrate, and phosphate in mucus than in surrounding water suggest its role in nutrient adsorption. In addition, coral mucus transports nutrients into reef sediment (Wild et al. 2005). Overall, coral mucus plays a crucial role in maintaining the health and productivity of reef ecosystems by facilitating nutrient cycling and supporting the diverse array of organisms that inhabit these ecosystems. Recycling these nutrients within the holobiont and the surrounding water helps us to maintain a balance of nutrients and supports the ecosystem’s productivity. Additionally, the role of coral mucus in nutrient cycling highlights the importance of conserving coral reefs and protecting them from human activities that may disrupt this delicate balance.
Conclusion
The coral surface mucus serves as an interface between the coral epithelium and the seawater environment. It provides a primary line of defense and protects coral surfaces from a variety of physio-chemical and microbial invasions. It acts as a high-energy substrate for bacterial heterotrophic activity. As both host and endosymbiont provide mucus constituents, this symbiotic mucus synthesis is different from many other marine organisms. The exact mechanism of mucus synthesis, secretion, and sources of other important components present in mucus is unknown, and less information is available about the properties and rheological changes of mucus, which are not fully demonstrated. Only certain coral species have been studied for their mucus components, such as carbohydrates, fatty acids, amino acids, proteins, antioxidants, and antimicrobial compounds, but even among those, not all types have been thoroughly explored. Also, mucus defense properties, including antimicrobial peptides, antioxidants, and UV protectants (e.g., Mycosporine-like amino acids), and beneficial microbes, are not well described for their roles in supporting coral health in various stress conditions. Coral mucus serves multiple ecological roles with their associated microorganisms and the surrounding environment that need to be studied to understand reef ecosystem functioning. Thus, the present review aids in understanding the role of coral mucus in coral reef ecosystems; however, more detailed studies of coral mucus components and their roles in the coral reef ecosystem by developing appropriate biochemical and molecular approaches may provide insights into protecting and restoring these valuable ecosystems.
Data availability
All data analysis of the present study are drawn from numerous previous published articles.
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Acknowledgements
The authors thank the Director, CSIR-National Institute of Oceanography for the facilities, including Ministry of Environment, Forest and climate change (MoEFCC), Govt. of India, New Delhi and Forest Department, Principal Chief Conservator of Forests (PCCF) and Chief wildlife warden, Chennai-15, Tamil Nadu and Wildlife Warden, Gulf of Mannar Marine National Park, Tamil Nadu, India, for the entry and as well as for collecting the samples. his is NIO contribution # 7164.
Funding
This study was supported by the Department of Biotechnology (DBT), (Grant No. BT/PR15162/AAQ/3/752/2015) India to Dr. C.R.. PVB was supported by CSIR (UGC) (Grant No.20/12/2015(ii)EU-V).
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PVB wrote the original draft, performed data analysis. CR Conceptualization, fund acquisition, writing and editing the original draft, performed data analysis. LI wrote the original draft, performed data analysis. All authors revised and approved the final manuscript.
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Bhagwat, P.V., Ravindran, C. & Irudayarajan, L. Beneficial properties of mucus in coral adaptations and ecological interactions. Mar Biol 171, 46 (2024). https://doi.org/10.1007/s00227-023-04372-4
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DOI: https://doi.org/10.1007/s00227-023-04372-4