Abstract
In cows, there is a drastic metabolic stress caused by violations of the rumen microbiome composition during lactation. The aim of the present study was to investigate the α-and β-diversity of the rumen microbiome of lactating cows using next-generation sequencing (NGS) and gene expression assessed by qPCR, as well as evaluation of the corrective properties of a probiotic strain introduced into the rumen. The results showed that the addition of the probiotic to the diet contributed to an effective increase in the fat content of milk during the early lactation period (P ≤ 0.05). The NGS-based analysis of the rumen microbiome of the studied cows using the 16S rRNA gene sequences showed that the Shannon and Chao1 indices of α-diversity of prokaryotic communities remained unchanged during various feeding phases, as well as due to the probiotic effects. In the early lactation period and under the probiotic's impact, the number of representatives of the Clostridia_UCG-014 and Clostridiaceae families declined by 2.4 and 1.6 times, respectively (in Group 2 as compared to Group 1, P ≤ 0.05). Expression of the bacterial Ldh-L and ldhD genes was lower by 2.9 and 13.5 times, respectively (P ≤ 0.05), when adding the probiotic to the diet in the early lactation period.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Rodionov, G.V., Kostomakhin, N.M., Tabakova, L.P.: Cattle breeding. Textbook (2020). (In Russ.)
Giniyatullin, Sh.Sh.: Feeding of cows by lactation periods and organization of cows’ milking. Russ. Electron. Sci. J. 1(19) (2016). (In Russ.)
Plaizier, J.C., Danesh Mesgaran, M., Derakhshani, H., Golder, H., Khafipour, E., Kleen, J.L., Lean, I., Loor, J., Penner, G., Zebeli, Q.: Review: enhancing gastrointestinal health in dairy cows. Animal 12(2), 399–418 (2018). https://doi.org/10.1017/S1751731118001921
Monteiro, H.F., Faciola, A.P.: Ruminal acidosis, bacterial changes, and lipopolysaccharides. J. Anim. Sci. 98(8), skaa248 (2020). https://doi.org/10.1093/jas/skaa248
Fu, Y., He, Y., Xiang, K., Zhao, C., He, Z., Qiu, M., Hu, X., Zhang, N.: The role of rumen microbiota and its metabolites in subacute ruminal acidosis (sara)-induced inflammatory diseases of ruminants. Microorganisms 10(8), 1495 (2022). https://doi.org/10.3390/microorganisms10081495
Steele, M.A., Croom, J., Kahler, M., Al Zahal, O., Hook, S.E., Plaizier, K., McBride, B.W.: Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis. Am. J. Physiol. Regul., Integr. Compar. Physiol. 300(6), R1515–R1523 (2011). https://doi.org/10.1152/ajpregu.00120.2010
Bannink, A., Gerrits, W.J.J., France, J., Dijkstra, J.: Variation in rumen fermentation and the rumen wall during the transition period in dairy cows. Anim. Feed Sci. Technol. 172(1–2), 80–94 (2012). https://doi.org/10.1016/j.anifeedsci.2011.12.010
Uyeno, Y., Shigemori, S., Shimosato, T.: Effect of probiotics/prebiotics on cattle health and productivity. Microbes Environ. 30(2), 126–132 (2015). https://doi.org/10.1264/jsme2.ME14176
Kalashnikov, A.P., Fisinin, V.I., Shcheglov, V.V., Pervoe, N.G., Kleimenov, N.I., Strekozov, N.I., Kalyshtsky, B.D., Egorov, I.A., Makhaev, E .A., Dvalishvili, V.G., Kalashnikov, V.V., Vladimirov, V.L., Gruzdev, N.V., Mysik, A.T., Balakirev, N.A., Fitsev, A.I., Kirilov, M.P., Krokhina, V.A., Naumepko, P.A., Vorobieva, SV., Trukhachev, V.I., Zlydnev, N.E., Sviridova, T.M., Levakhin, V.I., Galiev, B.Kh., Arilov, A.N., Bugdaev, I.E.: Norms and diets for feeding farm animals. 3rd edn. Moscow, Russia (2003). (In Russ.)
Makartsev, N.G.: Feeding farm animals: textbook for universities. Kaluga, Russia (2012). (In Russ.)
Nekrasov, R.V., Golovin, A.V., Makhaev, E.A., Anikin, A.S., Pervov, N.G., Strekozov, N.I., Mysik, A.T., Duborezov, V.M., Chabaev, M.G., Fomichev, Yu.P., Gusev, I.V.: Nutrient Requirements for Dairy Cattle and Pigs. Russia, Moscow (2018). (In Russ.)
QIIME 2. https://docs.qiime2.org/2020.8/. Accessed 15 January 2023
SILVA. https://www.arb-silva.de/documentation/release-138.1/. Accessed 15 Jan 2023
Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25(4), 402–408 (2001). https://doi.org/10.1006/meth.2001.1262
Posit Connect. https://rstudio.com. Accessed 15 Jan 2023
Chiquette, J., Allison, M.J., Rasmussen, M.A.: Prevotella bryantii 25A used as a probiotic in early-lactation dairy cows: effect on ruminal fermentation characteristics, milk production, and milk composition. J. Dairy Sci. 91(9), 3536–3543 (2008). https://doi.org/10.3168/jds.2007-0849
Tian, R., Ning, D., He, Z., Zhang, P., Spencer, S.J., Gao, S., Shi, W., Wu, L., Zhang, Y., Yang, Y., Adams, B.G., Rocha, A.M., Detienne, B.L., Lowe, K.A., Joyner, D.C., Klingeman, D.M., Arkin, A.P., Fields, M.W., Hazen, T.C., Stahl, D.A., Alm, E.J., Zhou, J.: Small and mighty: adaptation of superphylum Patescibacteria to groundwater environment drives their genome simplicity. Microbiome 8(1), 51 (2020). https://doi.org/10.1186/s40168-020-00825-w
Qiu, Q., Gao, C., Aziz, Ur Rahman, M., Cao, B., Su, H.: Digestive ability, physiological characteristics, and rumen bacterial community of Holstein finishing steers in response to three nutrient density diets as fattening phases advanced. Microorganisms 8(3), 335 (2020). https://doi.org/10.3390/microorganisms8030335
Ransom-Jones, E., Jones, D.L., McCarthy, A.J., McDonald, J.E.: The Fibrobacteres: an important phylum of cellulose-degrading bacteria. Microb. Ecol. 63(2), 267–281 (2012). https://doi.org/10.1007/s00248-011-9998-1
Flint, H.J., Duncan, S.H., Scott, K.P., Louis, P.: Links between diet, gut microbiota composition and gut metabolism. Proc. Nutrit. Soc. 74(1), 13–22 (2015). https://doi.org/10.1017/S0029665114001463
Acknowledgements
The work was carried out within the framework of state task No. 1022041400153-7-2.8.1 with the financial support of the Ministry of Agriculture of the Russian Federation.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Laptev, G. et al. (2023). Effect of a Probiotic Strain Administration in Different Feeding Phases on α- and β-diversity and Gene Expression of the Rumen Microbiome in Lactating Cows. In: Ronzhin, A., Kostyaev, A. (eds) Agriculture Digitalization and Organic Production. ADOP 2023. Smart Innovation, Systems and Technologies, vol 362. Springer, Singapore. https://doi.org/10.1007/978-981-99-4165-0_17
Download citation
DOI: https://doi.org/10.1007/978-981-99-4165-0_17
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-99-4164-3
Online ISBN: 978-981-99-4165-0
eBook Packages: Intelligent Technologies and RoboticsIntelligent Technologies and Robotics (R0)