Abstract
Intestinal parasitic infections (IPIs) remain a widespread public health concern causing severe implications in both developed and developing countries. Globally, numerous studies have been carried out ranging from various communities to schoolchildren as well as indigenous communities. The infections are commonly caused by helminths (e.g. Ascaris lumbricoides, Trichuris trichiura and hookworm) and protozoa (e.g. Blastocystis hominis, Cryptosporidium sp., Entamoeba histolytica and Giardia duodenalis). Poor sanitation and poverty are some of the factors associated with IPIs. With the ever-increasing impact of IPIs, newer detection approaches have been developed and studied. The efficacy of diagnostic method is crucial to give an accurate identification of these parasites. Recent developments of diagnostic tools such as serology- and molecular-based assays are assisting the conventional method of microscopy in detecting and further confirming current or past infections and the specific species of parasites. Ongoing investigations in parasitic infections using these advanced tools will provide useful information that will enable the evaluation of the effectiveness of the current control program and thus, assist future planning for improved strategies in eradicating these parasitic infections.
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1 Introduction
The world’s population has long been threatened by infectious diseases throughout the centuries. Currently, intestinal parasites are one of the major contributors to the global disease burden with a wide range of parasites that are reported to be prevalent around the world (Mehraj et al. 2008; Pullan et al. 2014; Mama and Alemu 2016), especially in sub-Saharan Africa, USA and Asia. Although these parasites are highly reported in underdeveloped countries, the emergence of intestinal parasitic infections (IPIs) has continued to compromise the quality of human life in developed nations. Infections are commonly caused by helminths (e.g. Ascaris lumbricoides, Trichuris trichiura and hookworm) and protozoa (e.g. Blastocystis hominis, Cryptosporidium sp., Entamoeba histolytica and Giardia duodenalis) resulting in significant morbidity and mortality, especially in endemic countries (Haque 2007). An estimation of 2 billion people are infected with intestinal parasites (Chan 1997) and the numbers have rapidly increased each year with 4 billion reported to be at risk in acquiring infections (Hotez et al. 2014). Extreme poverty, poor sanitation and social stigma as well as lack of education on the prevention and treatment are some of the factors contributing to these diseases (Liese et al. 2010). Furthermore, Basuni et al. (2012) had stated that the effects caused by IPIs depended on the species of parasites, the affected organ and the host immunological status. Although IPIs rarely cause death, the infection can impair the physical and mental growth, particularly among children (Varkey et al. 2007).
The challenges that arise due to the elevation of parasitic diseases have propelled newer, advance approaches and opportunities towards parasites diagnosis (Elsheikha 2014). In addition, latest techniques should be less time-consuming without compromising the quality of results. Therefore, rapid diagnosis is crucial and remained a top priority in determining the accurate identification of the parasites and eventually providing appropriate treatment as well as preventing fatalities among patients (Tavares et al. 2011). This mini review paper will briefly discuss the techniques commonly used in laboratory diagnosis with each method having its own advantages and disadvantages. The methods that are used for the diagnosis of several parasites that caused IPIs are further summarized in Table 1 as tabulated by Ndao (2009) and Ricciardi and Ndao (2015) with additional data by Wang et al. (2016).
1.1 Advancements in Parasite Diagnosis
For the past decades, various tests had been developed to increase the specificity and sensitivity in identifying parasites. The advancement of knowledge and technology had catapulted the diagnosing of parasitic infections to a new level. These techniques have been employed in numerous studies throughout the world, hence, enabling disease-combating efforts (Ricciardi and Ndao 2015).
1.2 Microscopy-Based Approach
Routine laboratory diagnosis that includes conventional microscopy technique has been widely used for morphological identification of parasites and was then the only tool for the detection of parasites obtained from cerebrospinal fluid, faeces, blood smears and tissue specimens (Tavares et al. 2011). This method is commonly employed as it requires inexpensive reagents or dyes and using only the microscope alone. However, throughout the years, although microscopy examination is considered as a gold standard, it is rather difficult to determine or distinguish the species through naked eye as it is largely dependent on an experienced microscopist to ensure quality results and it consumes time to process starting from sample collection to concentration of the parasite’s identification (Jamil et al. 2016). This situation can be further proven on the inability to distinguish E. histolytica and E. dispar through only morphological observation. Despite the disadvantage, dual techniques such as formalin-ether sedimentation, trichrome and Ziehl-Neelsen staining are usually applied together as conducted by Ngui et al. (2011) and Shahrul Anuar et al. (2013).
In addition, Kato-katz and McMaster counting methods are also common nowadays and regarded as a standard technique for the detection and quantification of IPIs for nearly forty years as reported by Komiya and Kobayashi (1966), Uga et al. (2002) and Belizario et al. (2015) and had since been recommended by WHO (1991). Meanwhile, McMaster counting method is extensively used to assess soil-transmitted helminths or STHs. It is also rather usual to include McMaster and Kato-katz technique in the same study as stated by previous studies (Pullan et al. 2010; Geiger et al. 2011; Periago et al. 2015). A study done by Levecke et al. (2011) revealed Kato-katz was more sensitive in detecting A. lumbricoides but not for T. trichiura and hookworm. Both methods were reported to have a considerable variation in sensitivity between different trials as Kato-Katz method covers larger quantity of stool but its drawback is when the infection intensity is rather low (Kongs et al. 2001) while McMaster technique is based on eggs flotation. Furthermore, both techniques are valid for diagnosis of IPIs with the latter being more suitable for further standardization due to its robust factor (Levecke et al. 2011).
1.3 Serology-Based Approach
Indirect identification of parasites using serology-based technique is employed if the parasite density is low or is unable to be directly demonstrated due to its life cycle in the host such as Toxoplasma gondii (Ambrosio and Waal 1990). The development of serology-based approach allows for faster and more practical diagnosis of IPIs that further provides an additional insight together with microscopic observation of the parasites. Serology-based diagnosis is further divided into two categories namely antigen detection assays and antibody detection assays that include enzyme-linked immunosorbent assay (ELISA), hemagglutination (HA) test, indirect or direct immunofluorescent antibody (IFA or DFA), complement fixation (CF) test and immunoblotting and rapid diagnostic tests (RDTs) (Ndao 2009). ELISA test is the most popular antibody detection assay in laboratory diagnosis while dipstick assays have also considered to be a more practical choice due to its simplicity and achieved higher sensitivity as compared to microscopy in detecting intestinal schistosomiasis (Sousa-Figueiredo et al. 2013).
Other serology-based assays namely indirect hemagglutination (IHA) and indirect immunofluorescence (IIF) are commonly performed in laboratories due to its sensitivity but limited studies had been conducted to analyse their reproducibility (Lescure et al. 2010). Furthermore, immunoassays have also become a main tool in diagnosing parasites (Castelino 1986; Okangba et al. 2010). For the detection of Giardia and Cryptosporidium, several commercial kits available in the market use immunoassay-based technique to test the parasites using FITC-monoclonal antibodies that target cell wall antigens (Ricciardi and Ndao 2015). The results from the assay is easier to interpret and consume less time to perform the test. However, the disadvantage of serology-based approach is that the diagnosis is retrospective due to the presence of antibodies that varies in different periods after infection occurred (Ndao 2009; Ricciardi and Ndao 2015).
1.4 Molecular-Based Approach
Polymerase chain reaction (PCR) method has become an important tool in the quantification of parasites as well as determining the efficacy of treatment process. This approach offers greater sensitivity and specificity in comparison to the current diagnostic examinations. With the advancement of technology, traditional PCR has evolved to nested, multiplexed and real-time PCR. For protozoan infections, PCR assay has successfully detected Cryptosporidium from the environmental samples by targeting the 18S rRNA (Johnson et al. 1995). In addition, multiplex real-time PCR assay used to detect E. histolytica, G. lamblia and C. parvum/C. hominis was reported to be comparable to microscopy as mentioned by ten Hove et al. (2007) and allows to detect multiple sequences simultaneously within the same reaction tube. Previous study carried out by Basuni et al. (2011) has successfully detected four species of soil-transmitted helminths namely Ancylostoma, N. americanus, A. lumbricoides and Strongyloides stercoralis through a pentaplex real-time PCR method. Meanwhile, nested PCR has revealed 100% of sensitivity and specificity for the detection of Taenia solium DNA by targeting the TSO31 gene (Mayta et al. 2008). Other previous findings showed that real-time PCR has proven to be sensitive in detecting Giardia and Cryptosporidium (oo)cysts (Guy et al. 2004; Gasser 2006). Conventional PCR-based method is rather time-consuming and does not provide quantitative data (Lin et al. 2000). However, although cost is a problem for multiplex PCR and real-time PCR, both have given rapid response as compared to the conventional method (Tavares et al. 2011). Furthermore, restriction fragment length polymorphism (RFLP) is also one of the most commonly used approaches in diagnosing parasites such as Toxoplasma gondii (Quan et al. 2008; Tavares et al. 2011). This technique is based on the digestion of PCR products by restriction enzymes and proven to be suitable for environmental samples as it is able to detect multiple genotypes from the same sample (Monis and Andrews 1998).
Besides PCR-based method, several other amplification techniques have also been developed. Notomi et al. (2000) have introduced a novel gene amplification technique, loop-mediated isothermal amplification (LAMP) that has been used in numerous studies. The advantages of LAMP technique are in its ability to amplify DNA with high efficiency under isothermal conditions and highly specific for the target sequence (Notomi et al. 2000). The method is also deemed simple and easy to perform as it only requires four primers, DNA polymerase and a regular laboratory water bath or heat block for reaction (Tavares et al. 2011). In addition, with the combination of reverse transcription, LAMP is able to amplify RNA sequences with high efficiency. Moreover, reagents can be kept at room temperature without any post-PCR steps as mentioned in Ricciardi and Ndao (2015). LAMP has been applied for the detection of both DNA and RNA viruses such as West Nile and SARS viruses as stated from previous study by Parida et al. (2004) and Poon et al. (2005). Previous study was also carried out to compare LAMP with multiplex PCR from stool samples of patients with taeniasis (Nkouawa et al. 2010). Meanwhile, a recent study by Imai et al. (2017) reported a novel diagnostic approach in identifying human Plasmodium species by combining LAMP and MinION sequencer method.
In addition, proteomics work has also rapidly expanded in recent years in analysing proteins expressed by the parasites (Boersema et al. 2015). The current interest in proteomics had led researchers to overcome limitations of early diagnosis and treatment (Petricoin et al. 2002) and has since evolved in the need for sensitivity. The identification of proteins involved two approaches namely bottom-up and top-down. The top-down strategy involves a two-dimensional polyacrylamide gel electrophoresis (Ndao 2009). Several other diseases such as malaria (Nyunt et al. 2005), taeniasis (Deckers et al. 2008) and Chagas disease (Santamaria et al. 2014) have incorporated the study of proteomics. Another new approach named microsatellites consisted of simple sequence tandem repeats and have also been described in reports on parasites obtained from both humans and animals (Temperley et al. 2009) with the ability to mutate rapidly (Johnson et al. 2006). Microsatellites are considered useful genetic markers as it is highly polymorphic (Abdul-Muneer 2014). Meanwhile, Luminex-based assays have also emerged as a possible approach in diagnosing parasitic infections that combine flow cytometry, fluorescent beads, lasers and digital signal processing (Tavares et al. 2011; Chen et al. 2016). Luminex was performed in a study conducted by Bandyopadhyay et al. (2007) that is able to differentiate C. hominis and C. parvum species by a single nucleotide. The differentiation of both species cannot be distinguished by using antigen detection or other serology tests. The study of the assay improves speed, accuracy and reliability of other PCR methods (Tavares et al. 2011).
1.5 Challenges in Management of Parasitic Infections
Our human body is constantly exposed to parasites daily from our surroundings causing diseases to occur. Intestinal parasites which were once considered as harmless commensals are now shown to be potential pathogens (Lukes et al. 2015). Nowadays, it is quite a trend among researches to focus on improving the current diagnostic techniques rather than inventing a new method, hence, with further improvement of the procedures, more parasites can be detected simultaneously. The useful feature of mass screening and rapid diagnostic will improve the understanding of the parasites as well as to reduce transmission of disease (Yansouni et al. 2014). Besides, the development of field-based diagnosis is also necessary to avoid critical delays. However, sensitivity and specificity as well as cost are still an issue. Renewed and sustainable intervention must be carried out especially in endemic regions. There are various ways that can be implemented to enhance the status of public health, notably in the field of medical parasitology, throughout the world such as by incorporating proper guidelines or policies, monitoring, evaluating and strengthening parasitic disease surveillance (Colley 2000; CDC CDC 2012). There is a crucial need for the monitoring of anti-parasite drugs resistance and other alternatives in developing better treatment for patients. Improved awareness such as regular deworming (Traversa 2012) and other preventive measures need to be carried out consistently especially in targeted areas. Finally, increasing the funding towards parasitological research and interventions is also needed to improve and eradicate potential pathogens (Zilungile et al. 2012).
2 Conclusion
Microscopy-based technique still remains as a useful tool in diagnosing patients with parasitic infections, despite the overwhelming development of new approaches. However, serology- and molecular-based methods are considered as excellent alternatives especially in low range of parasitic infections. The ongoing investigations and current available techniques in detecting the diseases provide a better platform in developing more efficient, reliable and inexpensive methods, hence, improving the quality of life as well as future reductions in global disease burden. The implementation of these recommendations requires full commitment from higher authorities that includes public health and healthcare agencies, medical professionals and funding providers. The support from all stakeholders will boost the efforts in combating IPIs. Future studies need to be carried out to further narrow the major gaps in science.
References
Abdul-Muneer PM (2014) Application of microsatellite markers in conservation genetics and fisheries management: recent advances in population structure analysis and conservation strategies. Genet Res Int 2014. https://doi.org/10.1155/2014/691759
Ali IKM, Haque R, Siddique A et al (2012) Proteomic analysis of the cyst stage of Entamoeba histolytica. PLoS Neglected Trop Dis 6(5):e1643
Ambrosio RE, De Waal DT (1990) Diagnosis of parasitic diseases. Sci Tech Rev Office Int des Epizooties 9(3):759–778
Bandyopadhyay K, Kellar KL, Moura I et al (2007) Rapid microsphere assay for identification of Cryptosporidium hominis and Cryptosporidium parvum in stool and environmental samples. J Clin Microbiol 45:2835–2840
Basuni M, Muhi J, Othman N et al (2011) A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths. Am J Trop Med Hyg 84(2):338–343
Basuni M, Mohamed Z, Ahmad M et al (2012) Detection of selected intestinal helminths and protozoa at Hospital Universiti Sains Malaysia using multiplex real-time PCR. Trop Biomed 29(3):434–442
Belizario VY Jr, Liwanag HJ, Naig JR et al (2015) Parasitological and nutritional status of school-age and preschool-age children in four villages in Southern Leyte, Philippines: Lessons for monitoring the outcome of Community-Led Total Sanitation. Acta Trop 141:16–24
Boersema PJ, Kahraman A, Picotti P (2015) Proteomics beyond large-scale protein expression analysis. Curr Opin Biotechnol 34:162–170
Bueno EC, Scheel CM, Vaz AJ et al (2005) Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay. Am J Trop Med Hyg 72:278–283
Bungiro RD Jr, Cappello M (2005) Detection of excretory/secretory coproantigens in experimental hookworm infection. Am J Trop Med Hyg 73:915–920
Castelino JB (1986) Immunodiagnosis of parasitic infections (Tools are evolving to help control diseases afflicting over 900 million people): atoms for better health care. IAEA Bull:15–19
CDC (2012) Centers for disease control and prevention global health strategy 2012-2015. https://www.cdc.gov/globalhealth/strategy/pdf/cdc-globalhealthstrategy.pdf. Accessed 18 Apr 2017
Chan MS (1997) The global burden of intestinal nematode infections—fifty years on. Parasitol Today 13:438–443
Chen T-H, Lee F, Lin Y-L et al (2016) Development of a multiplex Luminex assay for detecting swine antibodies to structural and non-structural proteins of foot-and-mouth disease virus in Taiwan. J Microbiol Immunol Infect 13:196–207
Cnops L, Soentjens P, Clerinx J et al (2013) A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travellers and migrants. PLoS Neglected Trop Dis 7(8):e2413
Colley DG (2000) Parasitic diseases: opportunities and challenges in the 21st century. Mem Inst Oswaldo Cruz, Rio de Janeiro 95(1):79–87
Danciger M, Lopez M (1975) Numbers of Giardia in the feces of infected children. Am J Trop Med Hyg 24:237–242
Deckers N, Dorny P, Kanobana K et al (2008) Use of ProteinChip technology for identifying biomarkers of parasitic diseases: the example of porcine cysticercosis (Taenia solium). Exp Parasitol 120(4):320–329
Del Brutto OH, Rajshekar V, White AC Jr et al (2001) Proposed diagnostic criteria for neurocysticercosis. Neurology 57:177–183
Elsheikha HM (2014) The future of parasitology: challenges and opportunities. Front Vet Sci 1(25)
Fotedar R, Stark D, Beebe N et al (2007) Laboratory diagnostic techniques for Entamoeba species. Clin Microbiol Rev 20:511–532
Garcia LS, Shimizu RY (1997) Evaluation of nine immunoassay kits (Enzyme Immunoassay and Direct Fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J Clin Microbiol 35:1526–1529
Gasser RB (2006) Molecular tools—advances, opportunities and prospects. Vet Parasitol 136(2):69–89
Geiger SM, Alexander ND, Fujiwara RT et al (2011) Necator americanus and helminth co-infections: further down-modulation of hookworm-specific type 1 immune responses. PLoS Neglected Trop Dis 5:e1280
Gonin P, Trudel L (2003) Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and Enzyme-linked Immunosorbent Assay. J Clin Microbiol 41:237–241
Guy RA, Xiao C, Horgen PA (2004) Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens. J Clin Microbiol 42(7):3317–3320
Haque R (2007) Human intestinal parasites. J Health Popul Nutr 25(4):387–391
Hira PR, Iqbal J, Al-Ali F et al (2001) Invasive amebiasis: challenges in diagnosis in a non-endemic country (Kuwait). Am J Trop Med Hyg 65:341–345
Hotez PJ, Alvarado M, Basanez M-G et al (2014) The global burden of disease study 2010: interpretation and implications for the neglected tropical diseases. PLoS Neglected Trop Dis 8(7):e2865
Imai K, Tarumoto N, Misawa K et al (2017) A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION nanopore sequencer. BMC Infect Dis 17:621
Jamil R, Waqas A, Sarfraz R et al (2016) Comparison of microscopic method and immune-chromatographic technique in detecting Plasmodium species. Biomedica 32(1):21–24
Johnson DW, Pieniazek NJ, Griffin DW et al (1995) Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl Environ Microbiol 61:3489–3855
Johnson PC, Webster LM, Adam A et al (2006) Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat. Mol Biochem Parasitol 148(2):210–218
Johnston SP, Ballard MM, Beach MJ et al (2003) Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens. J Clin Microbiol 41:623–626
Jothikumar N, da Silva AJ, Moura I et al (2008) Detection and differentiation of Cryptosporidium hominis and Cryptosporidium parvum by dual TaqMan assays. J Med Microbiol 57:1099–1105
Karanis P, Thekisoe O, Kiouptsi K et al (2007) Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Appl Environ Microbiol 73:5660–5662
Katz N, Chaves A, Pellegrino J (1972) A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14(6):397–400
Komiya Y, Kobayashi A (1966) Evaluation of Kato’s thick-smear technic with a cellophane cover for helminth eggs in feces. Jpn J Med Sci Biol 19:59–64
Kongs A, Marks G, Verle P et al (2001) The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Tropical Med Int Health 6:163–169
Levecke B, Behnke JM, Ajjampur SSR et al (2011) A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths. Plos Neglected Trop Dis 5(6):e1201
Lescure FX, Le Loup G, Freilij H et al (2010) Chagas disease: changes in knowledge and management. Lancet Infect Dis 10:556–570
Liang SY, Chan YH, Hsia KT et al (2009) Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica. J Clin Microbiol 47:1892–1895
Liese B, Rosenberg M, Schratz A (2010) Programmes, partnerships, and governance for elimination and control of neglected tropical diseases. Lancet 375(9708):67–76
Lin MH, Chen TC, Kuo TT et al (2000) Real-time PCR for quantitative detection of Toxoplasma gondii. J Clin Microbiol 38(11):4121–4125
Lodh N, Naples JM, Bosompem KM et al (2014) Detection of parasite-specific DNA in urine sediment obtained by filtration differentiates between single and mixed infections of Schistosoma mansoni and S. haematobium from endemic areas in Ghana. PLoS ONE 9(3):e91144
Lukes J, Stensvold CR, Jirku-Pomajbikova K et al (2015) Are human intestinal eukaryotes beneficial or commensals? PLoS Pathog 11(8):e1005039
Luna-Nacar M, Navarrete-Perea J, Moguel B et al (2016) Proteomic study of Entamoeba histolytica trophozoites, cysts, and cyst-like structures. PLoS ONE 11(5):e0156018
Mama M, Alemu G (2016) Prevalence and factors associated with intestinal parasitic infections among food handlers of Southern Ethiopia: cross sectional study. BMC Public Health 16:105
Mayta H, Gilman RH, Prendergast E et al (2008) Nested PCR for specific diagnosis of Taenia solium Taeniasis. J Clin Microbiol 46:286–289
Mehraj V, Hatcher J, Akhtar S et al (2008) Prevalence and factors associated with intestinal parasitic infection among children in an urban slum of Karachi. PLoS ONE 3(11):e3680
Monis PT, Andrews RH (1998) Molecular epidemiology: assumptions and limitations of commonly applied methods. Int J Parasitol 28(6):981–987
Ndao M (2009) Diagnosis of parasitic diseases: old and new approaches. Interdiscip Perspect Infect Dis. https://doi.org/10.1155/2009/278246
Ngui R, Ishak S, Chuen CS et al (2011) Prevalence and risk factors of intestinal parasitism in rural and remote West Malaysia. PLoS Neglected Trop Dis 5(3):e974
Nkouawa A, Sako Y, Li T et al (2010) Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans. J Clin Microbiol 48(9):3350–3352
Notomi T, Okayam G, Masubuchi H et al (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28(12):e63
Nyunt N, Pisciotta J, Feldman AB et al (2005) Detection of Plasmodium falciparum in pregnancy by laser desorption mass spectrometry. Am J Trop Med Hyg 73(3):485–490
Okangba CC, Oyibo WA, Obi RK et al (2010) Diagnosis of cryptosporidiosis in Africa: prospects and challenges. Adv Biores 1(1):34–40
Parida M, Posadas G, Inoue S et al (2004) Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1):257–263
Periago MV, Diniz RC, Pinto SA et al (2015) The right tool for the job: detection of soil-transmitted helminths in areas co-endemic for other helminths. PLoS Neglected Trop Dis 9(8):e0003967
Petricoin EF, Zoon KC, Kohn EC et al (2002) Clinical proteomics: translating benchside promise into bedside reality. Nat Rev Drug Discovery 1:683–695
Poon LLM, Wong BWY, Chan KH et al (2005) Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. J Clin Microbiol 43(7):3457–3459
Pullan RL, Bethony JM, Geiger SM et al (2010) Human helminth co-infection: no evidence of common genetic control of hookworm and Schistosoma mansoni infection intensity in a Brazilian community. Int J Parasitol 40:299–306
Pullan RL, Smith JL, Jasrasaria R et al (2014) Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasites Vectors 7(1):37
Quan JH, Kim TY, Choi IU et al (2008) Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis. Korean J Parasitol 46(2):105–108
Ricciardi A, Ndao M (2015) Diagnosis of parasitic infections: what’s going on? J Biomol Screen 20(1):6–21
Santamaria C, Chatelain E, Jackson Y et al (2014) Serum biomarkers predictive of cure in chagas disease patients after nifurtimox treatment. BMC Infect Dis 14:302
Shahrul Anuar T, Al-Mekhlafi HM, Abdul Ghani MK et al (2013) Evaluation of formalin-ether sedimentation and trichrome staining techniques: its effectiveness in detecting Entamoeba histolytica/dispar/moshkovskii in stool samples. J Microbiol Methods 92(3):344–348
Siddiki AZ (2012) Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimensional electrophoresis, image analysis and tandem mass spectrometry. Iran J Biotechnol 10(3):198–207
Sousa-Figueiredo JC, Betson M, Kabeterine NB et al (2013) The urine circulating cathodic antigen (CCA) dipstick: a valid substitute for microscopy for mapping and point-of-care diagnosis of intestinal schistosomiasis. PLoS Neglected Trop Dis 7(1):e2008
Tavares RG, Staggemeier R, Borges ALP et al (2011) Molecular techniques for the study and diagnosis of parasite infection. J Venomous Anim Toxins Incl Trop Dis 17(3):239–248
Temperley ND, Webster LM, Adam A et al (2009) Cross-species utility of microsatellite markers in Trichostrongyloid nematodes. J Parasitol 95(2):487–489
ten Hove RJ, Shuurman T, Kooistra M et al (2007) Detection of diarrhoea-causing protozoa in general practice patients in the Netherlands by multiplex real-time PCR. Clin Microbiol Infect 13(10):1001–1007
ten Hove RJ, Verweij JJ, Vereecken K et al (2008) Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal. Trans R Soc Trop Med Hyg 102(2):179–185
Traversa D (2012) Pet roundworms and hookworms: a continuing need for global worming. Parasites Vectors 5:91
Uga S, Kimura D, Kimura K et al (2002) Intestinal parasitic infections in Bekasi district, West Java, Indonesia and a comparison of the infection rates determined by different techniques for fecal examination. SE Asian J Trop Med Public Health 33:462–467
van Gool T, Vetter H, Vervoort T et al (2002) Serodiagnosis of imported shistosomiasis by a combination of a commercial indirect hemagglutination test with Schistosoma mansoni adult worm antigens and an enzyme-linked immunosorbent assay with S. mansoni egg antigens. J Clin Microbiol 40(9):3432–3437
Varkey P, Jerath AU, Bagniewski S et al (2007) Intestinal parasitic infection among new refugees to Minnesota, 1996-2001. Travel Med Infect Dis 5:223–229
Wang T, Zhao M, Rotgans BA et al (2016) Proteomic analysis of the Schistosoma mansoni miracidium. PLoS ONE 11(1):e0147247
Weber R, Bryan RT, Bishop HS et al (1991) Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods. J Clin Microbiol 29:1323–1327
WHO—World Health Organization (1991) Basic laboratory methods in medical parasitology. World Health Organization, Geneva, pp 25–28
Yansouni CP, Merckx J, Libman MD et al (2014) Recent advances in clinical parasitology diagnostics. Curr Infect Dis Rep 16:434
Young KH, Bullock SL, Melvin DM et al (1979) Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol 10:852–853
Zilungile MK, Musawenkosi M (2012) Status of medical parasitology in South Africa: new challenges and missed opportunities. Trends Parasitol 28(6):217–219
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Richard, R.L., Yusof, H. (2018). Advancements in Parasite Diagnosis and Challenges in the Management of Parasitic Infections: A Mini Review. In: Yacob, N., Mohd Noor, N., Mohd Yunus, N., Lob Yussof, R., Zakaria, S. (eds) Regional Conference on Science, Technology and Social Sciences (RCSTSS 2016) . Springer, Singapore. https://doi.org/10.1007/978-981-13-0074-5_64
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