Abstract
In processes where ionic concentrations vary significantly, the standard cable equation fails to accurately predict the transmembrane potential. Such processes call for a mathematical description able to account for the spatiotemporal variations in ion concentrations as well as the subsequent effects of these variations on the membrane potential. We here derive a general electrodiffusive formalism for consistently modeling the dynamics of ion concentration and the transmembrane potential in a one-dimensional geometry, including both the intra- and extracellular domains. Unlike standard cable theory, the electrodiffusive formalism accounts for diffusive currents and concentration-dependent variation of the longitudinal resistivities.
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Keywords
- Ion concentrations
- Spatiotemporal variations
- Membrane potential
- Electrodiffusive formalism
- Two-domain model
1 Introduction
In standard cable theory, the effect of ionic diffusion on the net electrical currents is neglected. Longitudinal resistivities, which in reality depend on ion concentrations, are assumed to be constant [6, 9, 12]. These are typically good approximations when modelling short-term electrical neural activity, as ion concentration typically vary little at the relevant time scale ( < 100 ms). However, in small intracellular volumes, such as dendritic spines, the local ion concentration can change quite dramatically within a few milliseconds [11], and in slower, macroscopic transport processes, concentration gradients may build up over time [2, 7]. For processes involving significant ion concentration gradients, the cable model will fail to give accurate predictions.
Qian and Sejnowski [11] have previously developed a consistent, electrodiffusive scheme for modelling the dynamics on v M and ion concentrations [11]. Like the standard cable model, the electrodiffusive model assumes that transport phenomena are essentially one-dimensional. Unlike the standard cable model, the electrodiffusive model derived v M from the ion concentration dynamics, accounting for all ionic movements (membrane fluxes, longitudinal diffusion, and longitudinal electrical migration), as well as for the concentration-dependent variation of the intracellular resistivities. An important limitation with this model [11] is that it only includes the dynamics in the intracellular space (ICS), whereas the extracellular space (ECS) was assumed to be isopotential and with constant ion concentrations.
Here expand the electrodiffusive formalim [11] to explicitly include both the ICS and the ECS. The result is a general mathematical framework for consistently modelling the dynamics of the membrane potential and ion concentrations in the intra- and extracellular domain. We believe that this framework will be of general value for the field of neuroscience. In the discussion, we give a few examples of processes that the formalism can be applied to.
2 Electrodiffusive Formalism
We seek a general mathematical framework for consistently modelling the dynamics of the membrane potential (v M ) and the concentrations in the ICS ([k] I ) and ECS ([k] E ) of a set (k) of ionic species in a geometry as that depicted in Fig. 1.
2.1 Particle Conservation
We consider the continuity equations for an ion species k with valence z k in domains I and E:
with the sealed-end boundary conditions (n = I or E):
Here a I and a E are the cross sections of the ICS and ECS, respectively, and O M is the circumference of the membrane. The longitudinal flux densities are given by the generalized Nernst-Planck equation (to keep notation short, we skip the functional arguments (x, t) from here on):
where the first term on the right represents the diffusive flux density (j kn d) and the last term is the flux density due to ionic migration in the electrical field (j kn f). The effective diffusion constant \(D_{k}^{{\ast}} = D_{k}/\lambda _{n}^{2}\) is composed of the diffusion constant D k in dilute solutions and the tortuosity factor λ n , which summarizes the hindrance imposed by the cellular structures [2, 10]. We use \(\psi = RT/F\), where R is the gas constant, T the absolute temperature, and F is Faraday’s constant. The formalism we derive is general to the transmembrane flux density (j kM ), as long as j kM is a local function of v M , ionic concentrations in I and E, and possibly some additional local state variables. The formalism can be combined with any external input (j kn in) which fulfills the constraint:
as we shall explain later.
With N ion species, Eqs. 1 and 2 (with j kn , j kM and j kn in as described above) represent a system of 2N + 3 variables which are functions of x and t. These are the 2N concentration variables ([k] n for \(k = 1,2,\ldots N\) and n = E, I), and the three additional variables (\(v_{M},\partial v_{I}/\partial x\) and \(\partial v_{E}/\partial x\)) occurring in the expressions for the flux densities. We now seek to express \(v_{M},\partial v_{I}/\partial x\) and \(\partial v_{E}/\partial x\) as functions of ionic concentrations, so that Eqs. 1 and 2 constitute a fully specified (and numerically solvable) system of equations.
2.2 Voltage Expressions
To reduce the number of independent variables to the 2N state variables ([k] n ) we use three additional constraints:
Equation 6 is the assumption that the membrane is a parallel plate capacitor. Then v M is determined by the density of charge on the inside of the membrane, which in turn is determined by the ionic concentrations:
For practical purposes, we have included a density of static charges (ρ sn ) in Eqs. 6 and 7, representing contributions from ions that are not considered in the conservation equations. If the set [k] n include all present species of ions, then ρ sn = 0.
As a capacitor separates a charge Q from a charge − Q (equal in magnitude, opposite in sign), the ECS charge density must be also consistent with v M . This is the second constraint (Eq. 7). To our knowledge, we are the first to make use of it in an electrodiffusive model. When using Eqs. 6 and 7, we implicitly assume that all local net charge in the system is stuck on the capacitive membrane (in the Debye-layers), and that the bulk solutions in the ICS and ECS are electroneutral [4, 8]. In order not to violate Eq. 7, the external input to the system must also be locally electroneutral (cf. Eq. 5).
We shall now use Eq. 7 together with the general definition of the transmembrane potential (Eq. 8) to derive our expressions for \(\partial v_{I}/\partial x\) and \(\partial v_{E}/\partial x\). We start by summing the particle conservation laws (Eqs. 1 and 2) to obtain:
If we multiply this by Fz k and take the sum over all ion species, k, the terms involving \(j_{kn}^{in}\) disappear due to Eq. 5 and the terms involving [k] n disappear due to Eqs. 7 and 9. We are left with:
The last implication follows from the sealed-end condition (Eq. 3). If the charge symmetry condition is satisfied at a given time t = 0, Eq. 11 is the condition that it remains satisfied at all times t.
The flux densities j kn are defined by Eq. 4. We note that Eq. 11 contains the sum of j kn over all ionic species. These sums can be converted to current densities. For convenience, we distinguish between the current densities due to diffusion (i n d) and migration in the electrical field (i n f), defined as [6]:
and
where r n denotes the resistivity [6]. With Eqs. 4, 12 and 13, Eq. 11 can be rewritten as:
By combining Eq. 8 with Eq. 14 we may finally derive our expressions for the voltage gradients:
Here, r n is given by Eq. 13, i n d by Eq. 12, and v M by Eqs. 6 and 9. All voltage terms are thereby expressed in terms of ionic concentrations, and the conservation equations (Eqs. 1 and 2) represent a fully specified system.
3 Discussion
We presented a one-dimensional, electrodiffusive framework for modeling the dynamics of the membrane potential (v M ) and the ion concentrations ([k] n ) of all included ion species (k) in an intra- and extracellular domain.
3.1 Implementation
A step-wise procedure of how to use this formalism is a follows:
-
1.
Specify initial conditions for the membrane potential (v M0) and k ion concentrations ([k] n0) that is to be simulated. The main charge carriers are believed to be Na+, K+ and Cl-, but other species can be included.
-
2.
Specify the static charge density (ρ sn ) so that [k] n0 and v M0 are consistent according to Eqs. 6 and 7.
-
3.
Specify the membrane mechanisms (functions for j kM ) representing ion channels, ion pumps and other membrane mechanisms relevant for the cell type that is to be modelled.
-
4.
Specify an external input function that fulfills Eq. 5.
-
5.
Solve the 2N conservation equations (Eqs. 1 and 2), with the boundary condition in Eq. 3. This can be done numerically by using a spatial discretization method (e.g., the Matlab-solver pdepe). For each time step, v M , \(\partial V _{I}/\partial x\) and \(\partial V _{E}/\partial x\) are defined algebraically by Eqs. 8, 15 and 16.
3.2 Applications
In the most direct interpretation, the ICS in Fig. 1 represents a single neurite coated with a thin sheath of ECS. For example, the ICS could represent an axon that has been removed from the ionic solution and placed in air or oil, so that only a thin layer of the ionic solution surrounds its membrane [12]. Alternatively, the ICS could represent an individual axon in e.g., the optical nerve, where axons are densely packed in bundles, and separated by constrained gaps of extracellular space [1]. However, cases where it is biologically meaningful to consider the ECS as a relatively thin coating faithfully following a single cell may be limited. For most single cell processes, the assumption that a I ≪ a E , and that conditions in the ECS are constant, may be more reasonable. In this limit the electrodiffusive formalism reduces to the one-domain (ICS) model presented previously by Qian and Sejnowski [11].
A geometrical simplification as that in Fig. 1 has also been justified for certain macroscopic transport processes through a chunk of neural tissue [2, 3, 5]. Typically, the ECS comprises about 20 % of the total neural tissue volume, while the remaining 80 % is the ICS of various cells [2]. Assuming that a large number of cells (e.g., all cells belonging to a specific species) participate in simultaneous ion exchange with the ECS, the impact on the ion concentrations in the ICS and ECS may be of the same order of magnitude. This calls for a two-domain formalism such as ours. When addressing a macroscopic transport process, the ICS in Fig. 1 does not refer to a single cell, but the total amount, within the chunk of tissue, of the participatory cell type. Similarly, ECS refers to the total extracellular volume in the chunk. For the geometrical parameters a E , a I and O M , one could then use, respectively, the ECS volume per total tissue volume, the ICS volume per total tissue volume, and the membrane surface area per total tissue volume. Such a macroscopic interpretation of Fig. 1 allows for a broader range of applications.
We have previously presented the electrodiffusive formalism with the specific application to a model of spatial potassium buffering by astrocytes [5]. We refer to the previous work for a specific, illustrative implementation.
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Acknowledgements
The project was supported by the Research Council of Norway (eVITA program; project numbers 178892 and 178901), and EU Grant 269921 (BrainScaleS).
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Halnes, G., Østby, I., Pettersen, K.H., Omholt, S.W., Einevoll, G.T. (2015). An Electrodiffusive Formalism for Ion Concentration Dynamics in Excitable Cells and the Extracellular Space Surrounding Them. In: Liljenström, H. (eds) Advances in Cognitive Neurodynamics (IV). Advances in Cognitive Neurodynamics. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9548-7_50
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