Abstract
Fruit set is a developmental process involving the transition of an ovary to a fruit. The process is generally stimulated by successful pollination and fertilization and is intricately controlled by plant hormones such as auxin and gibberellin. In tomato crop production, efficient fruit set is crucial for yield, and so parthenocarpy, or fruit set without pollination and fertilization, is a valuable commercial trait. In this study, we review fundamental and practical studies of fruit set and parthenocarpy, focusing on genes related to plant hormones as well as other factors, such as MADS-box transcription factors and anther development. We also propose strategies to genetically improve fruit set efficiency in tomato using molecular breeding techniques. Recently, genes and loci of potential use for improving fruit set have been discovered; however, more diverse genetic resources, as well as improved technical procedures (e.g., strict silencing of target genes in ovarian tissues), may still be required to develop diverse varieties that stably produce high-quality fruits under various environmental conditions. Further understanding of fruit set mechanisms and identification of the genes responsible for parthenocarpy in natural varieties will provide such resources and aid in designing future approaches to improve fruit set efficiency without introducing undesirable traits in reproductive and vegetative growth.
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7.1 Introduction
Breeding objectives have become more multifaceted over the centuries, but yield performance remains the most important trait for numerous crops. In fruit crops, yield is determined by the number and weight of fruits, and so efficient fruit set is essential for achieving good yield. Fruit set is a developmental process in which ovaries differentiate into fruits, and it is generally stimulated by successful pollination and fertilization, leading to embryo and seed development. Fruit set in tomato (Solanum lycopersicum) is very sensitive to environmental conditions and in particular to temperature because pollination is inhibited under excessively low or high temperatures (Iwahori and Takahashi 1963; Charles and Harris 1972). To avoid unfavorable conditions and achieve multiple cropping, greenhouse production is adapted to optimal conditions, but the maintenance of suitable temperatures is costly and consumes large amounts of energy. Greenhouse production also poses another problem for pollination in tomato, since closed greenhouses are barriers for wind- or pollinator-dependent flower vibration, which stimulates pollen diffusion from the anthers and hence self-pollination. Greenhouse growers often use insect pollinators, such as bumblebees, or hormone treatments to facilitate fruit set, but these methods are costly or time-consuming.
Parthenocarpy, or fruit set without pollination and fertilization, is a valuable trait for efficient tomato fruit production, in particular under unfavorable conditions for pollination and fertilization, such as during hot summers and cold winter weathers. Furthermore, parthenocarpic fruits are seedless, which is also generally desirable for both processing and fresh market production. In the processing industry, tomato seeds are usually removed from the paste, and seedless fruits often have longer shelf life than seeded fruits because seeds may produce ethylene, a ripening hormone (Varoquaux et al. 2000; Fei et al. 2004; Martinelli et al. 2009).
There is considerable evidence that fruit set is tightly controlled by several plant hormones, such as auxin, gibberellin (GA), and cytokinin (CK) (Gillaspy et al. 1993; Ruan et al. 2012; Ariizumi et al. 2013; McAtee et al. 2013), and application of these phytohormones to unpollinated tomato ovaries can induce parthenocarpic fruit set (Serrani et al. 2007a; Matsuo et al. 2012). In addition, several parthenocarpic tomato varieties have been developed, but most of the genes responsible for the traits remain unknown. The ovaries of major parthenocarpic varieties contain high amounts of GAs, even before pollination, and it has therefore been suggested that their parthenocarpy may be caused by altered hormonal activities (de Jong et al. 2009a). Tomato mutants and transgenic plants exhibiting parthenocarpy have also been developed, based on altered expression of hormone-related genes. Studies with Arabidopsis thaliana and tomato have led to model of fruit set mediated by auxin and GA (McAtee et al. 2013), wherein successful pollination and fertilization triggers the synthesis of auxin in the ovule. Auxin is then transported to the pericarp, where it induces de novo GA synthesis and the newly synthesized GA promotes pericarp expansion. In the early stages of tomato fruit development, CKs are also synthesized and they function to stimulate cell division (Matsuo et al. 2012). Application of N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU), a synthetic CK, to unpollinated tomato ovaries can induce parthenocarpic fruit development, and auxin and GA concentrations in the ovaries will transiently increase after CPPU treatment (Ding et al. 2013). Thus, modification of genes associated with CK biosynthesis and/or signaling pathway may lead to further understanding of the role of CK and the cross talk between CK and other plant hormones in fruit set.
Even though parthenocarpy represents a potentially valuable trait for many fruit crops, parthenocarpic varieties have been widely cultivated for only a few species (e.g., cucumber and banana) because high-quality fruits and a high yield are seldom combined with the parthenocarpic trait (Varoquaux et al. 2000; Pandolfini et al. 2002). This limitation is also true for tomato. In addition, the environment in which the fruits are cultivated has a major influence on parthenocarpy, and so the consistent control of the phenotypes in a commercial setting has proven difficult. These barriers have made it difficult to develop parthenocarpic varieties that stably produce high-quality fruits. Molecular techniques, such as genetic modification and marker-assisted selection (MAS), represent powerful tools for the development of new practical varieties showing parthenocarpy in the form of efficient introduction, selection, and pyramiding of useful genes. Here, we review studies of tomato fruit set and parthenocarpy, focusing particularly on parthenocarpic genes, loci, and variations associated with plant hormones and other components (Table 9.1). We also provide suggestions for genetically improving tomato fruit set using molecular breeding strategies. The induction of fruit set by modification of plant hormone-related genes is described, and we review other genetic modifications leading to fruit formation, such as MADS-box transcription factors, anther ablation, and the flavonoid synthesis pathways. Parthenocarpic variation developed through intervariety or interspecies crossing is also discussed.
7.2 Genetic Modifications and Mutations Influencing Fruit Set
7.2.1 Modifying the Expression of Genes Related to Auxin Synthesis, Responses, and Transport
The effect of auxin-related compounds on fruit set was first described in the early twentieth century (Gustafson 1936, 1937, 1939), and several natural and synthetic auxins were subsequently shown to induce parthenocarpic fruit set (Ho and Hewitt 1986). For example, application of the synthetic auxin, 4-chlorophenoxyacetic acid (4-CPA), can induce parthenocarpic fruits with comparable size to pollination-induced fruits (Bünger-Kibler and Bangerth 1982). More recently, several reports have described the induction of tomato parthenocarpy by altering the expression of several genes related to auxin metabolism and signaling (de Jong et al. 2009a; Ariizumi et al. 2013). For example, a chimeric gene, DefH9-iaaM, was used in early trials to produce transgenic parthenocarpic fruits in Solanaceae. This chimeric gene comprises the following two components: (1) the promoter of Deficiens homolog 9 (DefH9), which is an Antirrhinum majus MADS-box gene that is expressed specifically in the ovule, and (2) iaaM, derived from Pseudomonas syringae, which can induce auxin biosynthesis via synthesis of the indoleacetic acid (IAA) precursor indolacetamide. The expression of DefH9-iaaM was detected in the developing buds of the transgenic plants (Ficcadenti et al. 1999), which showed normal vegetative growth and developed fruits from emasculated flowers at a rate similar to that of pollinated flowers from non-transformed control plants. The fresh weights and soluble solid concentrations of the parthenocarpic fruits were unchanged or higher than the seeded fruits of control plants; however, the fruits produced by several transgenic lines were misshapen, likely because of excessive auxin levels (Pandolfini et al. 2002). To solve this problem, Pandolfini et al. (2002) altered the 5′ untranslated leader region (5′ ULR) of iaaM (DefH9-RI-iaaM), leading to a reduction in the translation efficiency of iaaM mRNA and, consequently, reducing the IAA content in flower buds compared with that in buds of the DefH9-iaaM transgenic plants. This resulted in optimal parthenocarpy, and when fruit productivity and quality of the DefH9-RI-iaaM transgenic plants were then evaluated under open-field conditions (Rotino et al. 2005), no obvious differences in fruit quality were detected, with the exception of a higher β-carotene level. The fresh weight of individual transgenic fruits was lower, but was compensated for by an increased fruit number, resulting in a yield comparable with that of parental non-transgenic lines. Expression of iaaM under the promoter of the ovule-specific INNER NO OUTER (INO) gene, which is expressed in only one cell layer in the ovule outer integument, has also been shown to induce parthenocarpy (Martinelli et al. 2009), but with fruits showing no significant morphological abnormalities.
Another chimeric gene, comprising Agrobacterium rhizogenes rolB fused to the ovary- and young-fruit-specific promoter, TPRP-F1, has also been used to generate parthenocarpic fruits (Carmi et al. 2003). Tomato plants transformed with rolB showed auxin-responsive phenotypes, although the underlying molecular mechanism is not yet known. The highest expression of rolB under the control of the TPRP-F1 promoter was detected in early developing fruits. The transgenic plants developed seedless fruits with a size and morphology comparable to those of seeded fruits from the parental lines, but fruit yield and several other qualities were different in greenhouse-cultivated plants. Expression of rolB under the control of the DefH9 and INO promoters also conferred parthenocarpy, but altered fruit qualities were observed (Martinelli et al. 2009).
AUXIN CUM SILENCING ACTION (AUCSIA) was identified as a gene that was repressed in parthenocarpic flower buds of DefH9-iaaM and DefH9-RI-iaaM transgenic plants (Molesini et al. 2009). The tomato genome has two AUCSIA genes (AUCSIA1 and AUCSIA2) that encode small polypeptides. RNA interference (RNAi)-mediated simultaneous suppression of AUCSIA1 and AUCSIA2 has been shown to result in parthenocarpy and an approximately 100-fold increase in total IAA content in the buds. The parthenocarpic fruit size and weight were smaller than those of wild-type fertilized fruits. AUCSIA1 and AUCSIA2 are highly expressed in flower buds, although these expression levels were substantially reduced after pollination (Molesini et al. 2009). The role of AUCSIA during fruit set is still unclear, but it might be involved in either auxin synthesis or transport.
PIN-FORMED (PIN) auxin efflux transporters play important roles in fruit set by controlling polar auxin transport between ovules and nearby tissues. Of the ten PIN genes (SlPIN1–SlPIN10) identified in tomato (Pattison and Catalá 2012), SlPIN4 has been shown to participate in fruit set (Mounet et al. 2012). SlPIN4 is predominantly expressed in flower buds and young developing fruits, where the expression level is higher in the placenta than in the locular tissue and pericarp. Specific silencing of SlPIN4 using an RNAi strategy resulted in parthenocarpy, suggesting a negative role in fruit set (Mounet et al. 2012).
7.2.2 Modifications and Mutations of Genes Associated with Auxin Signal Transduction
Auxin signaling is mediated by both transcription-dependent and -independent pathways, but only a few molecular components underlying the latter pathway have been identified (Mockaitis and Estelle 2008; Hayashi 2012). In the transcription-dependent pathway, protein–protein interactions among several key components lead to auxin responses. At low auxin levels, auxin/IAA (Aux/IAA) transcriptional repressors interact with auxin response factor (ARF) transcription factors, which repress the transcriptional expression of auxin-responsive genes (Tiwari et al. 2004; Guilfoyle and Hagen 2007). At high auxin levels, auxin promotes the degradation of the Aux/IAA proteins via a ubiquitin–proteasome system (Gray et al. 1999; Dharmasiri and Estelle 2002). Auxin is perceived by the TIR1/AFB family of F-box proteins acting as auxin receptors, which form SCF E3 ubiquitin ligase complexes, leading to the ubiquitination of Aux/IAA (Gray et al. 2001; Kepinski and Leyser 2005; Dharmasiri et al. 2005; Maraschin et al. 2009). Auxin-dependent proteolysis of Aux/IAA leads to the induction of auxin-responsive gene expression via activation of ARF transcription factors.
A total of 26 Aux/IAA repressor family genes (SlIAA1–SlIAA26) have been found in tomato (Wu et al. 2012). SlIAA9, which is highly expressed throughout the plant, has been show to play a regulatory role in fruit development, and antisense lines of SlIAA9 show a wide range of auxin-related growth alterations, including reduced leaf complexity and the production of parthenocarpic fruit with size, color, and flesh consistency that are similar to those of wild-type fruits (Wang et al. 2005). The accumulation of SlIAA9 transcripts at anthesis forms a gradient, where the transcript levels are higher in the ovule, sporogenous tissue, placenta, and funiculus but lower in the ovary wall and columella (Wang et al. 2009). Rapid dissipation of the signal gradient occurs approximately one day after pollination, suggesting an important role for SlIAA9 in the early stages of fertilization-induced fruit set. Three independent mutants of SlIAA9 (iaa9-3, iaa9-4, and iaa9-5) exhibiting altered vegetative phenotypes and parthenocarpy have been identified in ethyl methyl sulfonate (EMS)-mutagenized or γ-ray-irradiated populations (Saito et al. 2011). The rates of parthenocarpy and seedless fruit expansion vary among the mutants, suggesting that the functional activity and extent to which parthenocarpy is conferred vary for the different SlIAA9 alleles.
The tomato ARF family comprises at least 17 members (SlARF1–SlARF17; Kumar et al. 2011). SlARF7 is predominantly expressed in unpollinated tomato ovaries and its expression rapidly decreases after pollination (de Jong et al. 2009b). RNAi transgenic tomato lines with reduced SlARF7 mRNA levels produce parthenocarpic fruits, suggesting that this ARF gene acts as a negative regulator of fruit set. Furthermore, the parthenocarpic fruits showed GA-related phenotypes, such as a thick pericarp due to extensive cell expansion, in addition to auxin-related phenotypes, specifically a heart-shaped fruit and the formation of seedlike structures resembling pseudoembryos. These findings suggest that SlARF7 could be involved in the cross talk between auxin and GA during fruit set, and one model suggests that SlARF7 activates auxin response-attenuating genes (such as Aux/IAAs) in unpollinated ovaries, while downregulation of SlARF7 after pollination results in an activation of both auxin and GA signaling that is required for fruit set (de Jong et al. 2011).
In A. thaliana, atarf8 mutants produce parthenocarpic fruits (siliques) without fertilization, suggesting that AtARF8 is a negative regulator of fruit set (Goetz et al. 2006). Goetz et al. (2007) further showed that the introduction of aberrant forms of AtARF8 led to parthenocarpy in A. thaliana and tomato. Since the expression of AtARF8 was not reduced in the transgenic plants, the mutated form of the AtARF8 protein may have functionally competed with endogenous AtARF8 protein and its tomato homolog. A model was proposed by the authors in which ARF8 forms a regulatory complex with Aux/IAA, and this complex directly or indirectly represses transcription of fruit set-regulating genes (Goetz et al. 2006, 2007).
A putative tomato auxin receptor, SlTIR1, plays an important role in the early stage of fruit set (Ren et al. 2011). SlTIR1 is highly expressed in the ovary and sepal at anthesis, but its expression decreases after pollination. Overexpression of SlTIR1 results in an auxin-responsive phenotype, including altered vegetative morphology, sterility, and parthenocarpy, and SlTIR1 has been suggested to positively regulate the auxin response via the 26S proteasome-mediated signaling pathway (Ren et al. 2011).
7.2.3 Altered Expression and Mutations of GA-Related Genes
The regulatory effect of GAs on fruit development has been well documented (Wittwer et al. 1957; Sastry and Muir 1963; Serrani et al. 2007a). Endogenous GAs in plants are synthesized in two parallel pathways, the non-13-hydroxylation and early 13-hydroxylation pathways, and in tomato fruit set, the early 13-hydroxylation pathway appears to predominate (Bohner et al. 1988; Fos et al. 2000). Expression of genes in the tomato GA20ox family (SlGA20ox1–SlGA20ox3) that mediate bioactive GA synthesis increases in the ovaries after pollination, suggesting a central role for GA20ox genes in GA synthesis during fruit set (Serrani et al. 2007b). Additionally, overexpression of a citrus GA20ox gene (CcGA20ox1) in tomato resulted in pleiotropic phenotypes similar to those of GA-treated plants, including parthenocarpy (García-Hurtado et al. 2012).
DELLA family proteins act as key repressors of GA signaling. GA induces the degradation of DELLA proteins via the ubiquitin–proteasome system, leading to GA responses (Dill et al. 2001; Mcginnis et al. 2003; Sun 2010). Tomato has a single DELLA gene (SlDELLA), and antisense-mediated silencing of this gene results in constitutive GA-responsive phenotypes, such as elongated plant shape and parthenocarpy (Martí et al. 2007). In addition to being smaller and elongated, the parthenocarpic fruit had a reduced number of cells, which were elongated in the pericarp. These features are similar to those seen in GA-induced fruits (Serrani et al. 2007a), suggesting that parthenocarpic fruit development in SlDELLA antisense bypasses auxin-regulated cell division (Martí et al. 2007). A loss-of-function mutant of SlDELLA, procera (pro), also exhibits a constitutive GA-responsive phenotype, including parthenocarpy (Bassel et al. 2008). The pro mutation influences auxin signaling with a reduction of SlARF7 expression during fruit set (Carrera et al. 2012), suggesting a role of SlARF7 in the cross talk between GA and auxin signaling during fruit set.
7.2.4 Modification of the Expression of Ethylene-Related Genes
Ethylene plays a critical role in many developmental processes, such as senescence and abscission of leaves (Lim et al. 2007) and flowers (van Doorn and Woltering 2008), and fruit ripening (Barry and Giovannoni 2007). Llop-Tous et al. (2000) showed that pollination induces transient increases in the production of ethylene in tomato pistils for several hours, although this apparently does not induce ovary senescence (Vriezen et al. 2008) and ethylene production decreases after 12 h of pollination (Llop-Tous et al. 2000). The expression of various genes related to the biosynthesis and signaling of ethylene has been observed to change during the early development of both pollinated and parthenocarpic tomato fruits (Vriezen et al. 2008; Pascual et al. 2009; Wang et al. 2009), all suggesting that ethylene also plays a regulatory role in tomato fruit set; however, further studies are required to elucidate the exact mechanisms.
Overexpression of a tomato signaling component of ethylene (S. lycopersicum TETRATRICOPEPTIDE REPEAT PROTEIN 1, SlTPR1) that interacts with the ethylene receptors NEVER RIPE (NR) and S. lycopersicum ETHYLENE RECEPTOR 1 (SlETR1/LeETR1) results in ethylene-related pleiotropic effects and parthenocarpic fruit set (Lin et al. 2008). The upregulation of an auxin-responsive gene in the buds of SlTPR1-overexpressing plants suggests that SlTPR1 is directly or indirectly involved in auxin signaling, while downregulation of SlIAA9 in the ovaries of SlTPR1 transgenic plants may contribute to parthenocarpic fruit set. Further studies are required to clarify the role of SlTPR in ethylene signaling and fruit set.
The application of ethylene biosynthesis or action inhibitors has been found to induce parthenocarpy in zucchini (Cucurbita pepo), indicating that ethylene negatively regulates its fruit set (Martínez et al. 2013). In A. thaliana, ethylene is involved in ovule senescence and negatively regulates parthenocarpic fruit set induced by GA (Carbonell-Bejerano et al. 2011). Recently, either the treatment of 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, or ethylene-insensitive Sletr1 mutation was found to induce parthenocarpic fruit set from emasculated flowers, most likely due to the accumulation of bioactive GAs (Shinozaki et al. 2015). The efficiency of the pollination-independent fruit set induced by Sletr1 mutation was different depending on the genetic background, and unidentified locus/loci in a dwarf cultivar Micro-Tom, other than dwarf, can enhance the parthenocarpic efficiency. Ethylene may play a role in tomato fruit set by suppressing GA metabolism before pollination, while the molecular mechanism of the suppression has not yet been uncovered.
7.2.5 Altered Expression and Mutations of MADS-Box Transcription Factor Genes
MADS-box proteins are multifunctional transcription factors found in a wide range of eukaryotic organisms, and plant MADS-box proteins function in the regulation of organ and cell differentiation in flower development (Theissen and Saedler 2001). Tomato has at least 36 MADS-box genes (Hileman et al. 2006), and several studies have indicated a relationship between tomato parthenocarpic fruit development and MADS-box proteins. In A. thaliana, three MADS-box genes SEPALLATA1–SEPALLATA3 (SEP1–SEP3) are required for normal floral organ development (Pelaz et al. 2000). Studies with tomato have shown that Tomato MADS-box 29 (TM29), a homolog of A. thaliana SEP1, is continuously expressed in developing flowers and preferentially in the peripheral region of well-differentiated ovaries and fruits (Ampomah-Dwamena et al. 2002). Transgenic tomato plants constitutively expressing an antisense construct of TM29 produce morphologically altered flowers and parthenocarpic fruits, suggesting that TM29 not only is required for normal flower development but may also function as a negative regulator of fruit set. In apple (Malus pumila), antisense suppression of MADS8 and MADS9 (homologs of SEP1 and SEP2, respectively) resulted in an increased expression of auxin biosynthetic genes, a reduced expression of the GH3 auxin-conjugating enzyme genes, and a high accumulation of free auxin in the fruits during the early ripening stage (Schaffer et al. 2013). Although auxin concentration in the TM29-suppressed ovaries had not been measured, production of parthenocarpic fruits by silencing of these SEP family genes might be a consequence of the elevated auxin concentration.
TM5, a tomato ortholog of A. thaliana SEP3, is known to function in both flower and fruit development (Pnueli et al. 1994). TM5 is continuously expressed in the central apical zone of the floral meristem throughout differentiation in the tissues of petals, stamens, and pistils. Antisense suppression of TM5 expression resulted in parthenocarpy and altered identities of floral organs, manifested by sepaloid green petals and abnormal sterile anthers, but with no obvious change in vegetative organs (Pnueli et al. 1994). In addition, parthenocarpic fruit development resulted from a mutation in, or silencing of, the duplicated MADS-box genes, Tomato APETALLA3 (TAP3)/SlDEF and TM6 (de Martino et al. 2006). These genes belong to the AP3 group, a subfamily of class B MADS-box genes that are required for specification of petals and stamens. TAP3 is expressed predominantly in developing petals and stamen primordia until the late stage of flower differentiation, when the expression is restricted to several floral tissues. A tap3 null mutant was shown to have sepaloid petals and carpel-like anthers, and occasionally exhibited parthenocarpy. Recently, two stamenless (sl) mutants (slPr and sl-LA0269), which exhibit floral homeotic conversion to different degrees, were found to develop parthenocarpic fruits, and their phenotypes most likely resulted from mutation(s) in the coding sequence and promoter region, respectively, of TAP3 (Quinet et al. 2014). TAP3-antisense plants exhibited similar floral homeotic conversion to tap3 mutants, developing parthenocarpic fruits to which carpel-like stamens remained attached, and the severity was found to correlate with the extent of gene silencing. Similarly, TM6 is weakly but constitutively expressed in the primordia of petals, stamens, and carpels (de Martino et al. 2006), and TM6-RNAi transgenic plants are defective in stamen development and exhibit occasional parthenocarpy.
Even with efficient parthenocarpy and normal vegetative development, the floral reversion-like phenotype in the expression of downregulated or loss-of-function mutants of MADS-box genes described above leads to sterility, which limits their use for breeding. More spatially and temporally specific and/or more finely tuned regulation may be needed to develop practical parthenocarpic varieties using these genes. Both parthenocarpy and anther ablation were observed in the mutants and MADS-box gene transgenic plants described above. In the following section, we provide another example of a transgenic tomato line that supports the idea of a relationship between parthenocarpy and anther ablation.
7.2.6 Early Anther Ablation
It has been suggested that the stamens of A. thaliana play a regulatory role in preventing initiation of fruit in the absence of fertilization (Vivian-Smith et al. 2001). Parthenocarpy with male sterility has been reported in tomato parthenocarpic fruit (pat), a mutant derived from natural parthenocarpic variation (Mazzucato et al. 1998), implying that dysfunction of male organ, stamen, is involved in the parthenocarpic fruit development. Roque et al. (2007) showed that the induction of a Bacillus amyloliquefaciens ribonuclease gene (barnase) under the control of an anther-specific promoter from the Pisum sativum ENDOTHECIUM 1 (PsEND1) gene of pea resulted in specific ablation of the anther at early stages of the development and male sterility in A. thaliana, tobacco, oilseed rape, and tomato. Interestingly, tomato plants transformed with the PsEND1::barnase construct showed not only early anther ablation but also highly efficient parthenocarpy, while the other species completely impaired fruit development (Roque et al. 2007; Medina et al. 2013). The fruit productivity of the transgenic tomato plants was not significantly changed compared to wild-type plants, since although several transgenic lines produced smaller fruits, a significant increase in the number of fruits per plants was observed in all of the transgenic lines. Furthermore, PsEND1::barnase tomato plants produced high-quality fruits with respect to nutritional components such as γ-aminobutyric acid (GABA), glutamic acid, neoxanthin, and tocopherols. This approach in tomato crop production is attractive because it confers effective parthenocarpy without adverse effects on vegetative tissues. Further studies are required to understand the mechanisms underlying the link between early anther ablation accompanied by male sterility and parthenocarpic fruit development.
7.2.7 Modifications of Flavonoid Synthesis-Related Pathways
Genetic engineering of the flavonoid biosynthesis pathway represents yet another method to obtain parthenocarpic fruit. RNAi-mediated suppression of chalcone synthase (CHS), which encodes an important enzyme in flavonoid biosynthesis, has been reported to reduce total flavonoid levels and induce parthenocarpy (Schijlen et al. 2007). Transgenic plants showed normal vegetative growth, but their fruits were seedless and smaller than those of the seeded control fruits. Flavonoids play an essential role in reproductive processes such as pollen development and pollen tube growth. The parthenocarpic fruit development in the CHS RNAi plants appeared to be pollination associated, as pollen tube growth was impaired, and so fertilization was prevented. These findings suggest that pollination is required and sufficient to trigger fruit set and that fertilization leads to subsequent normal fruit development and expansion.
Seedless fruits with reduced flavonoid levels were also generated by introduction of the grape (Vitis vinifera) stilbene synthase (StSy), a key enzyme in the synthesis of the antioxidant resveratrol (Giovinazzo et al. 2005; Ingrosso et al. 2011). It was suggested that the altered flavonoid metabolism in StSy-transformed plants was caused by the competition between the biosynthetic pathways of resveratrol and chalcone and the parthenocarpic fruits contained high concentrations of soluble antioxidants, ascorbate and glutathione (Giovinazzo et al. 2005). Flowers of StSy-transformed plants displayed an open anther structure and were disturbed in pollen development, resulting in reduced seed set. The possibility that male sterility in the transgenic plants may be associated with its parthenocarpy was also suggested (Ingrosso et al. 2011; Medina et al. 2013).
7.3 Natural Variants and Introgression Lines Associated with Parthenocarpy
Several parthenocarpic variants have been developed for efficient fruit production, even under unfavorable conditions (Ho and Hewitt 1986), and three major natural variants for facultative parthenocarpy, pat, pat-2, and pat-3/pat-4, have been reported. In addition, two different lines exhibiting parthenocarpy have been described and used for quantitative trait locus (QTL) analysis (Gorguet et al. 2008). Identification of the associated genes will provide not only completely linked (so-called perfect) markers for parthenocarpy but also the identification of important pathways that confer parthenocarpy.
The pat mutant, harboring a recessive mutation in a single gene associated with parthenocarpy, was independently found in the tomato cultivars “Soressi” and “Montfavet 191.” The pat mutation results in several growth defects, including abnormal anthers, female gametes with lower viability and fewer seeds, and reduced fruit size and weight (Mazzucato et al. 1998). Altered development of stamens and parthenocarpy in the pat mutants suggests homeotic functions of PAT; however, while the pat locus has been mapped to the long arm of chromosome 3 (Beraldi et al. 2004), the candidate gene has not yet been identified.
The tomato cultivar “Severianin” exhibits strong parthenocarpy and its fruits reach almost normal size even under unfavorable conditions (Philouze and Maisonneuve 1978). “Severianin” was developed from crosses between several cultivars of tomato (S. lycopersicum) and the wild tomato species S. habrochaites. A genetic analysis suggested that the resulting parthenocarpy is controlled by two recessive loci, with major (pat-2) and minor (mp) effects (Vardy et al. 1989). Difference in yield and vigor has been observed in plants in which pat-2 was introduced, depending on the genetic background (Gorguet et al. 2008). Another strong parthenocarpic cultivar, “PR75/59,” was developed from a cross between the two weaker parthenocarpic cultivars, “Atom” and “Bubjekosoko,” and so two loci are thought to be responsible for the parthenocarpic phenotype, which have been designated pat-3 and pat-4 (Philouze 1989). However, the considerable variation in fruit size in pat-3/pat-4 makes this a less attractive cultivar for commercial production and hence for breeding.
The high accumulation of GAs in pat, pat-2, and pat-3/pat-4 ovaries may be associated with their parthenocarpic fruit set (Fos et al. 2000, 2001; Olimpieri et al. 2007). Olimpieri et al. (2007) showed that GA20ox1 is constitutively expressed in the ovaries of the pat mutant, while GA20ox3 has been shown by others to be highly expressed in pat-3/pat-4 ovaries (Pascual et al. 2009). In addition, the early growth of parthenocarpic pat-2 fruits is associated with polyamine metabolism. Polyamines are required for the parthenocarpy in pat-2, and that treatment of wild-type unpollinated tomato ovaries with exogenous polyamines induces partial parthenocarpy, proposing a model that the elevated GA content in the pat-2 ovaries induces polyamine synthesis (Fos et al. 2003).
In a QTL analysis using two tomato introgression lines (IL5-1 and IVT-line 1) carrying S. habrochaites chromosome segments and exhibiting highly stable parthenocarpy (Gorguet et al. 2008), four QTLs associated with parthenocarpy on chromosomes 4 (pat4.1, pat4.2), 5 (pat5.1), and 9 (pat9.1) were found. The two QTLs, pat4.1 from IL5-1 and pat4.2 from IVT-line 1, are allelic and closely linked to SlARF8, indicating that this gene is potentially responsible for parthenocarpy in these ILs, providing new target loci for breeding.
7.4 Perspectives
Parthenocarpic tomato fruits can be artificially produced using chemicals or via genetic modification. Although parthenocarpic varieties may contribute to efficient fruit production, as well as reductions in labor and cost for many crops, they have not yet been widely introduced in tomato cultivation, mainly because of a lack of genetic resources. The parthenocarpic plants described above provide possible resources for the development of commercially viable tomato varieties; however, several parthenocarpic genotypes exhibit undesirable traits, such as small-sized fruit and morphological defects. Techniques targeting spatial and temporal regulation of gene expression at adequate levels, for instance, using promoters that function specifically in ovarian tissues such as ovule, pericarp, and placenta during early stages of fruit development, may be helpful in eliminating such undesirable traits. In addition, the examples of varied phenotypes among mutant alleles of SlIAA9 (Saito et al. 2011) and TAP3 (Quinet et al. 2014) indicate that screening or generating various mutant alleles of genes associated with fruit set, via techniques such as targeting-induced local lesions in genomes (TILLING) (Gady et al. 2009; Okabe et al. 2011) and genome editing (Osakabe and Osakabe 2015), may contribute to increasing genetic variation and obtaining better breeding resources for parthenocarpic cultivars. Until now, only a few parthenocarpic genotypes have been evaluated for productivity performance, such as fruit quality, vegetative phenotypes, and yield, in commercial field environments that include exposure to low and high temperatures. To identify appropriate breeding resources, further practical trait evaluation is necessary. Parthenocarpic pro plants often show severe fruit malformation under greenhouse conditions (Carrera et al. 2012), but we have found that a different mutated allele of DELLA results in fewer defects in morphology of the parthenocarpic fruits under both moderate- and high-temperature condition compared with the pro mutant (unpublished data). This illustrates the necessity and benefit of trait evaluation in practice. Genes and loci with potential for improving tomato fruit set are now emerging (Table 9.1), but genetic resources and understanding of the fruit set mechanisms are still incomplete. Although molecular pathways and genes that are required for fruit set, including those downstream of hormone signaling, are poorly understood, continuing advances in genetic, genomic, and molecular tools and associated bioinformatic platforms are likely to accelerate their discovery.
References
Ampomah-Dwamena C, Morris BA, Sutherland P, Veit B, Yao JL (2002) Down-regulation of TM29, a tomato SEPALLATA homolog, causes parthenocarpic fruit development and floral reversion. Plant Physiol 130:605–617
Ariizumi T, Shinozaki Y, Ezura H (2013) Genes that influence yield in tomato. Breed Sci 63:3–13
Barry CS, Giovannoni JJ (2007) Ethylene and fruit ripening. J Plant Growth Regul 26:143–159
Bassel GW, Mullen RT, Bewley JD (2008) procera is a putative DELLA mutant in tomato (Solanum lycopersicum): effects on the seed and vegetative plant. J Exp Bot 59:585–593
Beraldi D, Picarella ME, Soressi GP, Mazzucato A (2004) Fine mapping of the parthenocarpic fruit (pat) mutation in tomato. Theor Appl Genet 108:209–216
Bohner J, Hedden P, Bora-Haber E, Bangerth F (1988) Identification and quantitation of gibberellins in fruits of Lycopersicon esculentum, and their relationship to fruit size in L. esculentum and L. pimpinellfolium. Physiol Plant 73:348–353
Bünger-Kibler S, Bangerth F (1982) Relationship between cell number, cell size and fruit size of seeded fruits of tomato (Lycopersicon esculentum Mill.), and those induced parthenocarpically by the application of plant growth regulators. Plant Growth Regul 154:143–154
Carbonell-Bejerano P, Urbez C, Granell A, Carbonell J, Perez-Amador MA (2011) Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in Arabidopsis. BMC Plant Biol 11:84
Carmi N, Salts Y, Dedicova B, Shabtai S, Barg R (2003) Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary. Planta 217:726–735
Carrera E, Ruiz-Rivero O, Peres LEP, Atares A, García-Martínez JL (2012) Characterization of the procera tomato mutant shows novel functions of SlDELLA protein in the control of flower morphology, cell division and expansion and auxin-signaling pathway during fruit-set and development. Plant Physiol 160:1581–1596
Charles WB, Harris RE (1972) Tomato fruit-set at high and low temperatures. Can J Plant Sci 506: 497–506
de Jong M, Mariani C, Vriezen WH (2009a) The role of auxin and gibberellin in tomato fruit set. J Exp Bot 60:1523–1532
de Jong M, Wolters-Arts M, Feron R, Mariani C, Vriezen WH (2009b) The Solanum lycopersicum auxin response factor 7 (SlARF7) regulates auxin signaling during tomato fruit set and development. Plant J 57:160–170
de Jong M, Wolters-Arts M, García-Martínez JL, Mariani C, Vriezen WH (2011) The Solanum lycopersicum AUXIN RESPONSE FACTOR 7 (SlARF7) mediates cross-talk between auxin and gibberellin signalling during tomato fruit set and development. J Exp Bot 62:617–626
de Martino G, Pan I, Emmanuel E, Levy A, Irish VF (2006) Functional analyses of two tomato APETALA3 genes demonstrate diversification in their roles in regulating floral development. Plant Cell 18:1833–1845
Dharmasiri S, Estelle M (2002) The role of regulated protein degradation in auxin response. Plant Mol Biol 49:401–409
Dharmasiri N, Dharmasiri S, Weijers D, Lechner E, Yamada M, Hobbie L, Ehrismann JS, Jürgens G, Estelle M (2005) Plant development is regulated by a family of auxin receptor F box proteins. Dev Cell 9:109–119
Dill A, Jung HS, Sun T (2001) The DELLA motif is essential for gibberellin-induced degradation of RGA. Proc Natl Acad Sci USA 98:14162–14167
Ding J, Chen B, Xia X, Mao W, Shi K, Zhou Y, Yu J (2013) Cytokinin-induced parthenocarpic fruit development in tomato is partly dependent on enhanced gibberellin and auxin biosynthesis. PLoS One 8, e70080
Fei Z, Tang X, Alba RM, White JA, Ronning CM, Martin GB, Tanksley SD, Giovannoni JJ (2004) Comprehensive EST analysis of tomato and comparative genomics of fruit ripening. Plant J 40: 47–59
Ficcadenti N, Sestili S, Pandolfini T, Cirillo C, Rotino GL, Spena A (1999) Genetic engineering of parthenocarpic fruit development in tomato. Mol Breed 5:463–470
Fos M, Nuez F, García-Martínez JL (2000) The gene pat-2, which induces natural parthenocarpy, alters the gibberellin content in unpollinated tomato ovaries. Plant Physiol 122:471–480
Fos M, Proaño K, Nuez F, García-Martínez JL (2001) Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato. Physiol Plant 111:545–550
Fos M, Proan K, Alabadı D, Nuez F, Carbonell J, García-Martínez JL (2003) Polyamine metabolism is altered in unpollinated parthenocarpic pat-2 tomato ovaries. Plant Physiol 131: 359–366
Gady AL, Hermans FW, Van de Wal MH, van Loo EN, Visser RG, Bachem CW (2009) Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations. Plant Methods 5:13
García-Hurtado N, Carrera E, Ruiz-Rivero O, López-Gresa MP, Hedden P, Gong F, García-Martínez JL (2012) The characterization of transgenic tomato overexpressing gibberellin 20-oxidase reveals induction of parthenocarpic fruit growth, higher yield, and alteration of the gibberellin biosynthetic pathway. J Exp Bot 63:5803–5813
Gillaspy G, Ben-David H, Gruissem W (1993) Fruits: a developmental perspective. Plant Cell 5: 1439–1451
Giovinazzo G, D’Amico L, Paradiso A, Bollini R, Sparvoli F, DeGara L (2005) Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene. Plant Biotechnol J 3:57–69
Goetz M, Vivian-smith A, Johnson SD, Koltunow AM (2006) AUXIN RESPONSE FACTOR8 is a negative regulator of fruit initiation in Arabidopsis. Plant Cell 18:1873–1886
Goetz M, Hooper LC, Johnson SD, Rodrigues JCM, Vivian-Smith A, Koltunow AM (2007) Expression of aberrant forms of AUXIN RESPONSE FACTOR8 stimulates parthenocarpy in Arabidopsis and tomato. Plant Physiol 145:351–366
Gorguet B, Eggink PM, Ocaña J, Tiwari A, Schipper D, Finkers R, Visser RGF, van Heusden AW (2008) Mapping and characterization of novel parthenocarpy QTLs in tomato. Theor Appl Genet 116:755–767
Gray WM, Pozo JC, Walker L, Hobbie L, Risseeuw E, Banks T, Crosby WL, Yang M, Ma H, Estelle M (1999) Identification of an SCF ubiquitin–ligase complex required for auxin response in Arabidopsis thaliana. Genes Dev 53:1678–1691
Gray WM, Kepinski S, Rouse D, Leyser O, Estelle M (2001) Auxin regulates SCF TIR1-dependent degradation of AUX/IAA proteins. Nature 414:271–276
Guilfoyle TJ, Hagen G (2007) Auxin response factors. Curr Opin Plant Biol 10:453–460
Gustafson FG (1936) Inducement of fruit development by growth-promoting chemicals. Proc Natl Acad Sci USA 22:628–636
Gustafson FG (1937) Parthenocarpy induced by pollen extracts. Am J Bot 24:102–107
Gustafson FG (1939) The cause of natural parthenocarpy. Am J Bot 26:135–138
Hayashi K (2012) The interaction and integration of auxin signaling components. Plant Cell Physiol 53:965–975
Hileman LC, Sundstrom JF, Litt A, Chen M, Shumba T, Irish VF (2006) Molecular and phylogenetic analyses of the MADS-box gene family in tomato. Mol Biol Evol 23:2245–2258
Ho LC, Hewitt JD (1986) Fruit development. In: Atherton JG, Rudich J (eds) The tomato crop. Chapman and Hall, New York, pp 201–239
Ingrosso I, Bonsegna S, De Domenico S, Laddomada B, Blando F, Santino A, Giovinazzo G (2011) Over-expression of a grape stilbene synthase gene in tomato induces parthenocarpy and causes abnormal pollen development. Plant Physiol Biochem 49:1092–1099
Iwahori S, Takahashi K (1963) High temperature injuries in tomato. II. Effect of duration of high temperature on fruit setting and yield. J Jpn Soc Hortic Sci 33:67–74
Kepinski S, Leyser O (2005) The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature 435:446–451
Kumar R, Tyagi AK, Sharma AK (2011) Genome-wide analysis of auxin response factor (ARF) gene family from tomato and analysis of their role in flower and fruit development. Mol Genet Genomics 285:245–260
Lim PO, Kim HJ, Nam HG (2007) Leaf senescence. Annu Rev Plant Biol 58:115–136
Lin Z, Arciga-Reyes L, Zhong S, Alexander L, Hackett R, Wilson I, Grierson D (2008) SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development. J Exp Bot 59:4271–4287
Llop-Tous I, Barry C, Grierson D (2000) Regulation of ethylene biosynthesis in response to pollination in tomato flowers. Plant Physiol 123:971–978
Maraschin FS, Memelink J, Offringa R (2009) Auxin-induced, SCF(TIR1)-mediated poly-ubiquitination marks AUX/IAA proteins for degradation. Plant J 59:100–109
Martí C, Orzáez D, Ellul P, Moreno V, Carbonell J, Granell A (2007) Silencing of DELLA induces facultative parthenocarpy in tomato fruits. Plant J 52:865–876
Martinelli F, Uratsu SL, Reagan RL, Chen Y, Tricoli D, Fiehn O, Rocke DM, Gasser CS, Dandekar AM (2009) Gene regulation in parthenocarpic tomato fruit. J Exp Bot 60:3873–3890
Martínez C, Manzano S, Megías Z, Garrido D, Picó B, Jamilena M (2013) Involvement of ethylene biosynthesis and signalling in fruit set and early fruit development in zucchini squash (Cucurbita pepo L.). BMC Plant Biol 13:139
Matsuo S, Kikuchi K, Fukuda M, Honda I, Imanishi S (2012) Roles and regulation of cytokinins in tomato fruit development. J Exp Bot 63:5569–5579
Mazzucato A, Taddei AR, Soressi GP (1998) The parthenocarpic fruit (pat) mutant of tomato (Lycopersicon esculentum Mill.) sets seedless fruits and has aberrant anther and ovule development. Development 125:107–114
McAtee P, Karim S, Schaffer R, David K (2013) A dynamic interplay between phytohormones is required for fruit development, maturation, and ripening. Front Plant Sci 4:79
Mcginnis KM, Thomas SG, Soule JD, Strader LC, Zale JM, Sun T, Steber CM (2003) The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase. Plant Cell 15:1120–1130
Medina M, Roque E, Pineda B, Cañas L, Rodriguez-Concepción M, Beltrán JP, Gómez-Mena C (2013) Early anther ablation triggers parthenocarpic fruit development in tomato. Plant Biotechnol J 11:770–779
Mockaitis K, Estelle M (2008) Auxin receptors and plant development: a new signaling paradigm. Annu Rev Cell Dev Biol 24:55–80
Molesini B, Pandolfini T, Rotino GL, Dani V, Spena A (2009) Aucsia gene silencing causes parthenocarpic fruit development in tomato. Plant Physiol 149:534–548
Mounet F, Moing A, Kowalczyk M, Rohrmann J, Petit J, Garcia V, Maucourt M, Yano K, Deborde C, Aoki K et al (2012) Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development. J Exp Bot 63:4901–4917
Okabe Y, Asamizu E, Saito T, Matsukura C, Ariizumi T, Brès C, Rothan C, Mizoguchi T, Ezura H (2011) Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries. Plant Cell Physiol 52:1994–2005
Olimpieri I, Siligato F, Caccia R, Mariotti L, Ceccarelli N, Soressi GP, Mazzucato A (2007) Tomato fruit set driven by pollination or by the parthenocarpic fruit allele are mediated by transcriptionally regulated gibberellin biosynthesis. Planta 226:877–888
Osakabe Y, Osakabe K (2015) Genome editing in higher plants. In: Yamamoto T (ed) Targeted genome editing using site-specific nucleases. Springer, Tokyo, pp 197–205
Pandolfini T, Rotino G, Camerini S, Defez R, Spena A (2002) Optimisation of transgene action at the post-transcriptional level: high quality parthenocarpic fruits in industrial tomatoes. BMC Biotechnol 2:1
Pascual L, Blanca JM, Cañizares J, Nuez F (2009) Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3/pat4 parthenocarpic fruit set. BMC Plant Biol 9:67
Pattison RJ, Catalá C (2012) Evaluating auxin distribution in tomato (Solanum lycopersicum) through an analysis of the PIN and AUX/LAX gene families. Plant J 70:585–598
Pelaz S, Ditta GS, Baumann E, Wisman E, Yanofsky MF (2000) B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405:200–203
Philouze J (1989) Natural parthenocarpy in tomato. IV. A study of the polygenic control of parthenocarpy in line 75/59. Agronomie 9:63–75 (in French with English abstract)
Philouze J, Maisonneuve B (1978) Heredity of the natural ability to set parthenocarpic fruits in the Soviet variety Severianin. Tomato Genet Coop Rep 28:12–13
Philouze J, Buret M, Duprat F, Nicolas-Grotte M, Nicolas J (1988) Caractéristiques agronomiques et physico-chimiques de lignées de tomate isogéniques, sauf pour le gène pat-2 de parthénocarpie, dans trois types variétaux, en culture de printemps, sous serre plastique trés peu chauffée. Agronomie 8:817–828 (in French with English abstract)
Pnueli L, Hareven D, Broday L, Hurwitz C, Lifschitz E (1994) The TM5 MADS Box gene mediates organ differentiation in the three inner whorls of tomato flowers. Plant Cell 6: 175–186
Quinet M, Bataille G, Dobrev PI, Capel C, Gómez P, Capel J, Lutts S, Motyka V, Angosto T, Lozano R (2014) Transcriptional and hormonal regulation of petal and stamen development by STAMENLESS, the tomato (Solanum lycopersicum L.) orthologue to the B-class APETALA3 gene. J Exp Bot 65:2243–2256
Ren Z, Li Z, Miao Q, Yang Y, Deng W, Hao Y (2011) The auxin receptor homologue in Solanum lycopersicum stimulates tomato fruit set and leaf morphogenesis. J Exp Bot 62: 2815–2826
Roque E, Gómez MD, Ellul P, Wallbraun M, Madueño F, Beltrán JP, Cañas LA (2007) The PsEND1 promoter: a novel tool to produce genetically engineered male-sterile plants by early anther ablation. Plant Cell Rep 26:313–325
Rotino GL, Acciarri N, Sabatini E, Mennella G, Lo Scalzo R, Maestrelli A, Molesini B, Pandolfini T, Scalzo J, Mezzetti B et al (2005) Open field trial of genetically modified parthenocarpic tomato: seedlessness and fruit quality. BMC Biotechnol 5:32
Ruan Y-L, Patrick JW, Bouzayen M, Osorio S, Fernie AR (2012) Molecular regulation of seed and fruit set. Trends Plant Sci 17:656–665
Saito T, Ariizumi T, Okabe Y, Asamizu E, Hiwasa-Tanase K, Fukuda N, Mizoguchi T, Yamazaki Y, Aoki K, Ezura H (2011) TOMATOMA: a novel tomato mutant database distributing Micro-Tom mutant collections. Plant Cell Physiol 52:283–296
Sastry KKS, Muir RM (1963) Gibberellin: effect on diffusible auxin in fruit development. Science 140:494–495
Schaffer RJ, Ireland HS, Ross JJ, Ling TJ, David KM (2013) SEPALLATA1/2-suppressed mature apples have low ethylene, high auxin and reduced transcription of ripening-related genes. AoB Plants 5:pls047
Schijlen EG, de Vos CH, Martens S, Jonker HH, Rosin FM, Molthoff JW, Tikunov YM, Angenent GC, van Tunen AJ, Bovy AG (2007) RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits. Plant Physiol 144:1520–1530
Serrani JC, Fos M, Atarés A, García-Martínez JL (2007a) Effect of gibberellin and auxin on parthenocarpic fruit growth induction in the cv micro-tom of tomato. J Plant Growth Regul 26:211–221
Serrani JC, Sanjuán R, Ruiz-Rivero O, Fos M, García-Martínez JL (2007b) Gibberellin regulation of fruit set and growth in tomato. Plant Physiol 145:246–257
Shinozaki Y, Hao S, Kojima M, Sakakibara H, Ozeki-Iida Y, Zheng Y, Fei Z, Zhong S, Giovannoni JJ, Rose JKC et al. (2015) Ethylene suppresses tomato (Solanum lycopersicum) fruit set through modification of gibberellin metabolism. Plant J 83:237–251
Sun T (2010) Gibberellin-GID1-DELLA: a pivotal regulatory module for plant growth and development. Plant Physiol 154:567–570
Theissen G, Saedler H (2001) Plant biology. Floral quartets. Nature 409:469–471
Tiwari SB, Hagen G, Guilfoyle TJ (2004) Aux/IAA proteins contain a potent transcriptional repression domain. Plant Cell 16:533–543
van Doorn WG, Woltering EJ (2008) Physiology and molecular biology of petal senescence. J Exp Bot 59:453–480
Vardy E, Lapushner D, Genizi A, Hewitt J (1989) Genetics of parthenocarpy in tomato under a low temperature regime: II. Cultivar ‘Severianin’. Euphytica 41:9–15
Varoquaux F, Blanvillain R, Delseny M, Gallois P (2000) Less is better: new approaches for seedless fruit production. Trends Biotechnol 18:233–242
Vivian-Smith A, Luo M, Chaudhury A, Koltunow A (2001) Fruit development is actively restricted in the absence of fertilization in Arabidopsis. Development 128:2321–2331
Vriezen WH, Feron R, Maretto F, Keijman J, Mariani C (2008) Changes in tomato ovary transcriptome demonstrate complex hormonal regulation of fruit set. New Phytol 177:60–76
Wang H, Jones B, Li Z, Frasse P, Delalande C, Regad F, Chaabouni S, Latché A, Pech J-C, Bouzayen M (2005) The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell 17:2676–2692
Wang H, Schauer N, Usadel B, Frasse P, Zouine M, Hernould M, Latché A, Pech J-C, Fernie AR, Bouzayen M (2009) Regulatory features underlying pollination-dependent and -independent tomato fruit set revealed by transcript and primary metabolite profiling. Plant Cell 21:1428–1452
Wittwer SH, Bukovac MJ, Sell HM, Weller LE (1957) Some effects of gibberellin on flowering and fruit setting. Plant Physiol 32:39–41
Wu J, Peng Z, Liu S, He Y, Cheng L, Kong F, Wang J, Lu G (2012) Genome-wide analysis of Aux/IAA gene family in Solanaceae species using tomato as a model. Mol Genet Genomics 287:295–311
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Shinozaki, Y., Ezura, K. (2016). Tomato Fruit Set and Its Modification Using Molecular Breeding Techniques. In: Ezura, H., Ariizumi, T., Garcia-Mas, J., Rose, J. (eds) Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops. Biotechnology in Agriculture and Forestry, vol 70. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-48535-4_7
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