Abstract
Cd2+, Zn2+ and Cu2+ have expressed effect on QB protonation and reduction in bacterial reaction centers (BRC) (Utschig et al., 2001). This work is dedicated to investigating influence of Cd2+, Pb2+, Hg2+, Zn2+, and Cu2+ at the level of acceptor side of photosystem II (PS II). Light-dependent proton uptake decreased in the presence of 80 μM Cu2+ by 90%, and of 200 μM Zn2+—by 70%. At that, considerable reductions of electron transport rate were not observed. In the concentration range of 50–200 μM Cu2+ and Cd2+ caused an insignificant growth of [PSII-QB-non-reducing]rel, whereas in the presence of Zn2+ and Pb2+ this value grew more than by 25%. At all concentrations of ions of heavy metals the [PSII-QB-non-reducing]rel value did not exceed 60%. By their effect on dark relaxation of variable chlorophyll fluorescence, heavy metals used might be divided in two groups: (1) Cu2+ Hg2+, accelerating the fast and medium components of fluorescence decay; (2) Zn2+, Pb2+ and Cd2+, slowing down medium and fast components. An assumption was made that blocking the reaction of QB protonation by ions of heavy metals activates the alternative acceptor site in PS II.
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Polishchuk, O.V., Topchiy, N.M., Podorvanov, V.V. (2013). Activation of Alternative Electron Transfer in PS II by Inhibition of Proton Transfer at the Acceptor Side. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_42
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DOI: https://doi.org/10.1007/978-3-642-32034-7_42
Publisher Name: Springer, Berlin, Heidelberg
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