Abstract
pH of cell culture medium and dissolved oxygen are important criteria for cell proliferation and differentiation and are also crucial for the performance of cell culture systems. Therefore, this study aims to investigate the performance of a novel hydrostatic bioreactor while measuring the changes of pH and dissolved oxygen in bicarbonate-buffered and HEPES-buffered cell culture media. pH changes of media samples, which were subjected to hydrostatic pressure with varying pressure amplitudes, frequencies and exposure times, were determined via a colorimetric approach. The concentration of dissolved oxygen was measured using a micro optical oxygen sensor. An increase in pressure, frequency and time resulted in an increase on pH. However, after an exposure time of 30 minutes no further increase in pH was observed and the pH of the media samples were adjusted to the cell culture environment of the incubator. Similar trends were observed for bicarbonate-buffered and HEPES-buffered media. Initial results suggest further that pressure had a stronger influence on pH than frequency.
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© 2013 Springer-Verlag Berlin Heidelberg
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Reinwald, Y., El Haj, A.J. (2013). Evaluation of the Performance of a Novel Hydrostatic Bioreactor. In: Long, M. (eds) World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China. IFMBE Proceedings, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29305-4_518
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DOI: https://doi.org/10.1007/978-3-642-29305-4_518
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-29304-7
Online ISBN: 978-3-642-29305-4
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