Abstract
Castration of young male rats is performed with minimal bleeding in animals weighing less than 60 g. The animal is anesthetized. A small transversal incision is made in the skin on the ventral site over the symphysis. The testis lying in the scrotum is gently pushed into the abdominal cavity. With a pair of fine forceps, the abdominal wall is opened. The epididymal fat pad, easily seen, is grasped with the forceps, and the testis with the epididymis is pulled out from the wound. The ductus deferens with the testicular vessels is crushed with artery forceps and the testis together with the epididymal fad pad cut off with a pair of fine scissors. There is almost no bleeding in young animals. In older animals, ligation of the testicular vessels together with the ductus deferens may be necessary. The same procedure is performed on the other side. The skin wound is closed with one or two wound clips. The animal recovers immediately. With some skill, the operation can be performed very rapidly (Bomskov 1939).
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References and Further Reading
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Sandow, J. (2015). Testicular Steroid Hormones. In: Hock, F. (eds) Drug Discovery and Evaluation: Pharmacological Assays. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27728-3_78-2
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