Abstract
During the past several decades, anal physiology testing has afforded us a more complete understanding of the complex interactions between pelvic anatomy and physiology. Techniques have been improved and validated in both normal volunteers and in states of disease. This chapter will give a detailed overview of the differing methods of anal physiology testing and provide clinical correlations as to when these tests may be useful, or conversely, in which situations clinical judgment should supersede testing.
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Introduction
Throughout the past several decades, we have learned a great deal about the complex physiology of the distal rectum, pelvic floor, and anal canal. The majority of these discoveries have come through the advent of testing modalities including anal manometry, electromyography (EMG), cinedefecography, rectal compliance measurements, and measurements of specific anorectal reflexes. These testing modalities have led to a better understanding of the complex interplay between pelvic muscle and nerve functions as they relate to normal physiology as well as the ways that these mechanisms change in the setting of various disease states.
As knowledge of physiologic parameters has increased over time, the differing techniques have had ranges of “normal” values reported. Though these can be helpful guides in interpreting these studies, any given value needs to be evaluated in context because variations in measurement technique may provide differing results [1, 2]. It is most important for the surgeon to have knowledge of their own testing equipment and interpret testing values in the context of those typically seen with their own devices. Anal physiology testing has also allowed us to understand many different reflex arcs such as the bulbocavernosus reflex [3, 4], the cough reflex [5,6,7], cutaneous-anal reflex [8], the rectoanal excitatory reflex [9, 10], and rectoanal inhibitory reflex [11, 12]. Though most of these reflexes can be an important part of determining overall spinal nerve function, the rectoanal inhibitory reflex (RAIR) is the most relevant to the study of colorectal disease as it has been noted to affect such conditions as Hirschsprung’s disease [13] and fecal incontinence [14]. Similarly, its abolition after low anterior resection may be associated with many of the post-operative functional disorders that affect patients. In recent years, many of the techniques have been modified and enhanced with the addition of modalities such as magnetic resonance defecography (MR defecography) [15, 16], high resolution anal manometry [17, 18], and anal canal vector volume manometry [19].
This chapter will provide a broad overview of the techniques commonly used to evaluate anorectal and pelvic floor anatomy and physiology. We will first describe the techniques in detail and describe the interpretation of the results both in the instance of normal findings as well as in states of disease. Finally, we will address present day clinical correlations, and how testing methodology can be used to guide clinical decision-making, or conversely, in which instances clinical judgment should supersede the need for testing.
Techniques
Anorectal Manometry
Instrumentation and Technique
There are a variety of methods for performing anorectal manometry testing. The essential components involve a pressure measuring probe, pressure transducers, a recording component, and in the setting of water perfusion methods, a hydraulic pump. Many modern devices are now self-contained systems, offering advanced functionality (Fig. 3.1). The most common difference in setup is in the transducing catheter, where small balloons filled with air or water, water-perfusion catheters, and solid state catheters have been used [20]. Currently, the most commonly utilized transduction system uses a soft multichannel catheter, which is perfused with water or air. The unit then measures the pressure needed to overcome the sphincter pressure during various states such as resting or squeeze (Fig. 3.2).
A variety of techniques to measure pressures throughout the anal canal are used. Some techniques include stationary measurements, where the catheter is left in one location. However, the more common techniques involve slowly withdrawing the catheter from the rectum by hand. Many systems are using an automated rather than manual pullback method, including those, which use vector volume techniques (Fig. 3.3) [19, 21,22,23,24].
The standard pull through technique involves placing the catheter into the rectum until it is above the sphincter complex. Subsequently, resting and squeeze pressures are measured at each station, usually in 1 cm intervals. Directional pressures (anterior, posterior, and left or right lateral) can be measured at each station. Squeeze duration may also be measured to determine the stamina exhibited by the sphincter muscles. During this process, rectal compliance and the RAIR can also be elucidated [20].
The newest techniques are the vector volume manometry technique and high-resolution anal manometry. The vector volume technique involves a continuous pull through in which the system creates vector diagrams, which are used to generate a three dimensional reconstruction of anal canal pressures [19]. As algorithms have improved over the years, fairly accurate representations of anal canal squeeze pressure, resting pressure, length, and symmetry can be reasonably calculated (Fig. 3.4). The results of this technique suffer from a lack of generalizability, as there are a myriad of techniques and algorithms used for vector volume manometry .
Revaluation of standard manometry techniques utilizing a variety of measurement methods (water perfused side hole, water perfused end hole, microtransducer, or microballoon) have demonstrated relatively consistent results across platforms [20]. However, evidence suggests that vector volume manometry may yield higher estimations of anal canal pressures [25]. Yang et al. conducted a prospective analysis comparing vector volume manometry against standard pull through manometry in 50 consecutive patients with fecal incontinence. Their conclusion was that lower pressures may be measured during standard techniques because patients are given more time to rest between squeezes as opposed to the continuous pull through used in the vector volume methods [25]. These data suggest that surgeons need to become comfortable with the data generated by their own manometry system, and be cognizant of the fact that values generated on a given machine may not be directly correlated to external controls. Proponents of the vector volume imaging technique suggest that algorithms have improved over time and there is greater reproducibility in the results [19]. What is less clear is to what degree this technique adds clinical value over standard techniques, and whether it is cost-effective.
High-resolution manometry techniques were initially developed to investigate esophageal motility and have been adapted to study anorectal disease. This technique has the potential to generate 3-dimensional maps of pressure gradients throughout the anal canal (Fig. 3.5). Although only small pilot studies have examined this technique in the setting of various disease states [17, 26, 27], data is beginning to emerge on the suggested “normal” values of this technique when measured in healthy volunteers [18]. The challenge for future research is to determine whether this technique can offer more useful information than traditional techniques, or whether other techniques such as dynamic distensibility measurements may correlate better with various disease states [28].
Anal resting pressure receives as much as 55–85% of its contribution from the internal sphincter, while squeeze augmentation is mostly from the external sphincter [20, 29,30,31,32,33]. Studies of controls as well as patients with pelvic floor disorders have generated several “normal values ” (Table 3.1). Differences have been noted between different gender and age, with generally higher pressures in males and decreased pressures in elderly patients [34]. Pressures are relatively low in the anterior aspect of the upper third of the anal canal, which corresponds to the area not surrounded by the puborectalis sling, and in the posterior aspect of the lower third of the anal canal.
Anal canal length is also assessed by manometric measurement [35]. In many instances, the length of the anal canal has been shown to correlate with sphincter function, and can be predictive of outcomes in disease states such as fecal incontinence [35,36,37]. Length of the sphincter can almost more be construed as a physiologic, rather than an anatomic length. Many modern systems are now able to reproduce dynamic pressure tracing curves of the anal canal pressures at rest and during maneuvers such as squeeze and push (Figs. 3.6 and 3.7).
Balloon Expulsion
An inexpensive simple test for obstructed defecation is balloon expulsion . Many of the current generation manometry catheters are equipped with a balloon, which can be utilized for this purpose. Though multiple different patient positions and balloon inflation methods have been examined, asking the patient to lay in a supine position and expel a balloon with a 60 mL volume appears to be the most reproducible method (Fig. 3.8) [38]. However, one of the editors (DEB) prefers to place an air or water filled 60 cc Helium type balloon into the anus and have the patient sit on a commode to pass the balloon. This is less embarrassing and more physiologic to the passage of stool [39].
Some investigators have cited this test as a reliable means of ruling out pelvic floor dyssynergia in the setting of constipation [40,41,42,43]. Minquez et al. studied two groups of constipated patients (106 with functional constipation, and 24 with pelvic floor dyssynergia based upon manometry and defecography assessments.) Balloon expulsion testing was pathologic in 21 of 24 with pelvic floor dyssynergia and only 12 of 106 with functional constipation [41]. However, a more recent study by Kassis et al., demonstrated a sensitivity of 33% and positive predictive value of 71% of balloon expulsion testing in patients who were diagnosed with pelvic floor dyssynergia suggesting that balloon expulsion is only a complementary test to other modalities in the diagnosis of pelvic floor dyssynergia [40].
Electromyography
Electromyography (EMG) has been used to study both normal anatomy as well as sphincter muscle and pelvic floor muscle in various pathologic states such as fecal incontinence [44,45,46,47,48,49], paradoxical puborectalis contraction (Figs. 3.9 and 3.10) [51, 52], solitary ulcer syndrome [53], rectal prolapse [48], and perineal descent. One of the difficulties in interpretation of the literature regarding EMG is the multitude of techniques, which have been described. Based on the type of recording electrode used, there are four commonly described techniques available to evaluate pelvic floor muscles. These include concentric needle electrode, monopolar wire electrode, single fiber electrode and surface anal plug, which now include multi-channel devices.
Needle Electrode EMG
Older systems tended to utilize needle electrodes for EMG testing. These included the concentric needle, which is either a bare tipped 0.1 mm diameter steel wire which is introduced into the external anal sphincter to record electrical activity, or the softer monopolar wire EMG electrode, which was thought to give the same information as concentric needle EMG with less patient discomfort. The electrical activity is recorded from each of the four quadrants of the external sphincter complex to ensure accurate sphincter mapping [49, 54,55,56,57,58,59]. Although accurate measurements may be obtained at the single point that the needle is placed, individual muscle fiber function cannot be reliably tested in this manner. Single fiber electrode techniques improved on this, by providing a representation of the activity of individual muscle fibers within a motor unit. However, the needle EMG techniques are currently less commonly utilized. The most common of these uses was historically, sphincter mapping; however, endoanal ultrasound has largely replaced EMG for this purpose [60,61,62].
Surface Electrode EMG
Currently, the most common clinical application for EMG is in examining external anal sphincter activity and whether contraction and relaxation is occurring appropriately. This is easily accomplished using a surface electrode EMG. The anal plug consists of two longitudinal or circular silver wires mounted on a plastic or sponge surface. Though surface electrodes have been modified in recent years and can afford more accurate depictions of the morphology of the sphincter complex [44, 63,64,65], the most common use currently is in the diagnosis of paradoxical puborectalis contraction (anismus), or as a means of demonstrating muscular activity during biofeedback retraining [51]. The incidence of EMG-documented paradoxical puborectalis contraction in chronically constipated patients ranges from between 42 to 100 [66,67,68,69]. Patient embarrassment plays a significant role in accurate diagnosis of anismus, and functional testing via cinedefecography may be more accurate.
Rectal Pressure Testing (Manometry)
The role of the rectum in normal, healthy people is to act as social organ. It is a storage reservoir, one that accommodates stool without initiating the urge to defecate and subsequently allows defecation at a socially appropriate time. This is dependent upon the complex interplay between rectal distensibility and complex defecatory reflexes. Basal pressures within the rectum range from between 5 to 25 cm H2O (or 2–18 mmHg). The initial inflation of an intrarectal balloon is associated with an initial rise in pressure, often followed by a secondary increase in pressure due to rectal contraction. A degree of accommodation then occurs after which the rectal pressure gradually falls to a baseline value. Eventually, as intrarectal pressure increases above a certain threshold, a person will feel an urge to defecate. This threshold is different across individuals and can be affected by conditions, which reduce the capacity of the rectal vault such as low anterior resection [70], or conditions which reduce rectal compliance such as radiation proctitis [71], or ulcerative colitis [72]. The contractile response of the rectum to distention is decreased or absent in patients with spinal cord lesions, suggesting a spinal contribution to this reflex.
Rectal Capacity and Compliance
Rectal capacity determines the frequency of defecation. This is apparent in individuals who have had a low anterior resection for rectal cancer, where increased stool frequency is an expected functional consequence of surgery [73, 74]. Rectal compliance is responsible for the degree of urgency for evacuation. Some of the factors commonly utilized to determine rectal compliance are the rectal volume at first sensation, volume at first urge to defecate, and the maximum tolerable volume (MTV). These measurements are obtained utilizing a balloon attached to the end of the catheter and positioned inside the rectum. Most commonly, the balloon is distended with water and pressure measurements are recorded as cm of H2O. The water in the balloon is usually maintained at 37 °C, and should not be lower than room temperature, or higher than body core temperature. Prior to injecting water, the patient needs to be instructed on the purpose of the test, and informed of what is being asked of them. Baja et al. demonstrated that a single injection of water can be used with accurate results, and that the technique of multiple injections to permit “conditioning” appear to be unnecessary [75]. Commonly, the volume in the balloon at the first urge to defecate is recorded. Maximum tolerable volume is not often recorded due to patient discomfort. One of the only studies demonstrating utility in maximum tolerable volume demonstrated that patients with a maximum tolerable volume <60 cc had a high incidence of fecal incontinence [76], however, this predictive value was shown to be no better than the predictive value of anal manometry [77], thus, the added patient discomfort of this test is not justified. Rectal compliance, by definition, is 1/slope ΔP/ΔV. Put simply, compliance measures the response of the rectum (by change of pressure) in response to a change in volume [78,79,80]. Rectal compliance , measured as mL/cm H2O have been shown to vary, and normal values ranging from 5.1–15.7 mL/cm H2O have been reported [80]. Conditions associated with low rectal compliance include radiation proctitis and inflammatory bowel disease, while idiopathic constipation with megarectum may be associated with abnormally high compliance [80]. Recent evidence suggests that patients with impaired continence after anal fistulotomy may have impaired rectal compliance due to scarring in addition to diminished muscle pressures, and this may be an additional mechanism leading to incontinence [81]. Due to the wide range of values reported as normal in the literature, some have suggested that the accuracy of this measurement needs to be interpreted with caution due to limitations with the technique [80]. Other factors have been shown to impact rectal compliance measurements including the contribution of extrarectal tissues to the measurement, as well as differences in rectal size. Newer techniques have been developed to attempt to control for variations in rectal size such as the barostat technique, which uses a large volume bag (with infinite compliance to the limit of its capacity) to test rectal compliance . The proposed advantage of this technique is to attempt to control for variation in capacity. This can hopefully address the issue where a patient with a larger volume rectum will appear more compliant due to the volume of water it can accommodate, as opposed to basing this measurement simply on wall distensibility [78].
The Rectoanal Inhibitory Reflex (RAIR)
One notable aspect of the complex neuromuscular network of the anal canal is the rectoanal inhibitory reflex (RAIR) . Distention of the rectum leads to a consequent relaxation of the internal sphincter allowing the rectal contents access to the specialized sensory epithelium lining the upper anal canal. This mechanism allows for sampling and conscious or subconscious discrimination between solid, liquid, or gas contents [82]. Increasing degrees of rectal distention lead to complete internal sphincter inhibition. While the internal sphincter relaxes, the external sphincter contracts to maintain continence. During this episode, there is a small decrease in anal pressure noted: this normal reflex is what defines the RAIR [11, 83]. RAIR is thought to play an important role in maintenance of continence, as it facilitates “sampling” of rectal contents to the specialized sensory apparatus of the anal canal; this is what allows a person to distinguish between solid, liquid, and gas contents. The RAIR is mediated by nitric oxide and relies on the presence of the interstitial cells of Cajal to mediate its effect [84, 85]. RAIR is noted to be absent in conditions with an impaired myenteric nerve plexus such as Hirschsprung’s disease or Chagas’ disease, or after surgical resection of the rectum [11, 83, 86, 87].
The test is performed utilizing a balloon catheter. The balloon is placed 2 cm proximal to the anal verge. The expected result is a 50% drop in resting pressure in at least one channel in response to balloon insufflation. The test is interpreted as normal if this condition is met. If the RAIR is absent, this suggests impaired neuromuscular function, and disease states such as Hirschsprung’s disease, rectal prolapse, scleroderma, or dermatomyositis should be considered in the proper clinical context [86]. RAIR is also often absent in the setting of chronic rectal prolapse due to a neuropathy induced by chronic stretching of the prolapsed tissue, resulting in continuous stretching of the receptors.
Cinedefecography
Defecography is a technique utilized to assess the process of defecation in a dynamic manner (Fig. 3.11). The primary clinical indication is for the workup of obstructed defecation, or pelvic organ prolapse [88]. Through the past decades the imaging protocols have been modified, but the goal remains to assess the functional interaction of the pelvic floor during the defecatory process.
The patient is placed on the left lateral position, with instillation of 50 mL of liquid barium into the rectum, followed by insufflation of a small quantity of air. In addition, 100–200 mL of barium paste is injected into the rectum [89,90,91]. Though various other techniques for contrast administration (intravesical [92], oral [93], intravaginal barium soaked tampon [93], or intraperitoneal [94, 95]) have all been described, the most common configuration is rectal barium combined with a vaginal barium paste. This allows for accurate identification of additional pathology such as enterocele or vaginal vault prolapse, while minimizing patient discomfort and inconvenience [96,97,98]. Often, a radio-opaque marker is placed on the perineum, which allows measurement of perineal descent.
The patient is then seated on a commode, and lateral radiographs, both static and dynamic are obtained during the process of defecation. The patient is coached to attempt to recreate a normal bowel movement and evacuate the contents of the rectum as completely as possible.
X-ray images are recorded at rest, as well as during squeezing and pushing maneuvers.
Parameters commonly measured are the anorectal angle, degree of perineal descent, and whether paradoxical contraction of the puborectalis is observed [99, 100]. However, many other diagnoses such as internal or complete rectal prolapse, sigmoidocele, enterocele, rectocele, or vaginal vault prolapse may be demonstrated (Figs. 3.12 and 3.13).
The anorectal angle is the angle created between straight lines traversing the anal canal and the rectum. This angle is thought to be largely created by the function of the puborectalis muscle at rest. Normal values have been reported to be 90–110° at rest, and 110–180° at evacuation (Fig. 3.11) [99, 101, 102]. Though the examination of the dynamic change in anorectal angle in a given patient can be clinically useful, the absolute numbers generated are not very useful because there is disagreement among experts as to the suggested normal values. Though techniques vary, a common reference point for measurement is between the axis of the anal canal and the tangential line of the posterior rectal wall [103].
Perineal decent is measured in relation to a line drawn from the most anterior portion of the symphysis pubis to the coccyx (the pubococcygeal line) [104]. In general, the pelvic floor is observed to rise during squeeze maneuvers and descend with defecation. Perineal descent of more than 3 cm in the resting phase or an increase of more than 3 cm during the pushing phase are the definitions of fixed and dynamic perineal descent, respectively [105, 106]. Additionally, the degree of emptying of the rectum is assessed. Normal rectal emptying should take less than 30 s, and less than 10% of the contrast should remain in the rectum in order for emptying to be read as normal. The degree of emptying should be carefully assessed, as anatomic “abnormalities” can appear on defecography, and correlation of these findings to the patient’s symptoms as well as a dynamic analysis of the defecatory process is paramount. Shorvon et al. illustrated that in a mixed gender group of “normal” volunteers, half showed radiological evidence of mucosal prolapse and intussusception. Additionally, 17 of the 21 women studied also had evidence of a rectocele [107]. Other studies have corroborated these findings as well, suggesting that the mere presence of a rectocele on physical examination or defecography is not enough to warrant a repair [108,109,110,111,112,113]. We would suggest that symptoms of difficult evacuation, need for vaginal splinting to precipitate evacuation, and evidence of non-emptying of the rectocele on defecography should be requisite conditions which should be met prior to consideration of a rectocele repair. We will go into further detail correlating defecography findings to clinical outcomes later in this chapter.
Magnetic Resonance Defecography
More commonly, MRI technology is being utilized in the study of pelvic floor disorders [114,115,116,117,118,119,120,121,122]. Magnetic resonance (MR) defecography has been proposed as an alternative to fluoroscopic defecography. Proponents of this technique cite the absence of ionizing radiation and excellent depiction of anatomy. Detractors to this approach cite that the supine patient positioning may alter the normal physiologic process of defecation, which can only be recreated in the upright position. Ideally, an open MRI configuration would be utilized, allowing a patient to sit upright during the examination, however this equipment is not readily available in most institutions. The procedure is typically carried out using a 1.5 T MRI detector, and a body coil is used rather than an endorectal coil. Though fluoroscopic defecography still appears to be the gold standard radiographic test for pelvic floor disorders, a recent study by Vitton et al. demonstrated that MRI techniques are improving. Though the concordance rate of MRI to conventional defecography in diagnosing rectocele (82%), or enterocele (93%) were reasonably good, the concordance of MRI to standard defecography in diagnosing perineal decent was only 57% [123]. This is likely because the supine positioning of MRI is not able to reproduce perineal descent in an accurate manner. There is however, some evidence that MR may be better than cinedefecography at demonstrating internal prolapse [120]. Though MR techniques continue to emerge, the current gold standard modality for assessing pelvic floor function radiographically remains conventional cinedefecography.
Pudendal Nerve Terminal Motor Latency Testing (PNTML)
Pudendal nerve terminal motor latency testing has been performed for the past several decades in an attempt to determine whether the neuromuscular function of the pelvic floor is intact. The technique is accomplished by using a finger-mounted transanally inserted electrode (St. Marks electrode). The fingertip portion of the electrode contains the stimulating portion, while a sensor at the base of the finger measures the response (Figs. 3.14 and 3.15). The clinician places the fingertip on the pudendal nerve as it traverses over the ischial spine. The latency period between pudendal nerve stimulation and electromechanical response of the muscle is then measured. Generally, stimulation is checked bilaterally a total of two to three times to be sure that the measurement is reproducible.
Pudendal nerve function has been demonstrated to correlate with age, particularly in women [124,125,126,127]. Though it was previously thought that pudendal neuropathy (PN) correlated with abnormal perineal descent, emerging data suggests that this relationship may be confounded, as both perineal descent and PN are common in older age. PN has been demonstrated to occur in such varied conditions as fecal incontinence [45, 128,129,130], constipation [131,132,133,134,135], rectal prolapse [133,134,135], combined fecal and urinary incontinence [133], and low anterior resection syndrome [136, 137]. A study by Lim et al. in 2006 suggested that patients treated with neoadjuvant chemotherapy and radiation for rectal cancer may develop PN after treatment [138, 139]. The authors suggested that this may contribute to the development of low anterior resection syndrome (LARS) . More recently, Tomita et al. correlated postoperative PN to soiling and incontinent episodes following low anterior resection. Though PN was thought to be important, the factor most highly predictive of soiling was the height of the anastomosis, with lowest anastomoses producing the most severe symptoms. LARS will be discussed in greater detail below [136]. PN is also associated with traumatic vaginal delivery. Surprisingly, up to, 20% of women who undergo vaginal delivery without apparent injury to the external sphincter may also have prolonged pudendal nerve terminal motor latencies. Subsequent recovery occurs approximately in 15% of these patients [139]. Recently, Loganathan et al. also demonstrated that either unilateral, or bilateral PN predicts diminished resting and squeeze tone, even in patients who are found to have an intact internal and external sphincter complex [128].
The technique is interpreted by assessing the amount of time that is taken to elicit a motor response after stimulation of the pudendal nerves. Though different values are reported at different institutions, a normal PNTML is generally considered to be 2 ± 0.2 ms [67, 140]. Though some studies have reported higher “normal” values [1, 124, 141] a surgeon must interpret the results of this test in the context of values typically seen with their own equipment. Pudendal nerve studies are interpreted independently on the left vs. the right side. Additionally, the conduction curve should be examined to be sure that it is reproducible between one test and the next to ensure a reliable measurement of pudendal nerve function.
Clinical Considerations
While a detailed understanding of the various testing modalities is critical to the practice of pelvic floor evaluation, the utility of these tests is best understood in a clinical context. In the following section, we detail several common disease states that may benefit from pelvic floor evaluation. We will review commonly used tests and expected results to help frame the practical utility of pelvic floor testing. A detailed discussion of therapeutic intervention is beyond the scope of this chapter, but will be addressed elsewhere in the text.
Hirschsprung’s Disease
Hirschsprung’s disease , or congenital aganglionosis of the colon , was first detailed by Dr. Hirschsprung in 1887, when describing a detailed report of constipation in newborns due to dilation and hypertrophy of the colon [142]. In 1948, Zuelzer and Whitehouse identified the pathophysiology as aganglionosis in the rectum and distal bowel, which provided the first scientific basis for intervention [143, 144]. The diagnosis of Hirschsprung’s is usually made early after birth, due to the lack of passage of meconium, prompting appropriate surgical intervention. In a small segment of the population, however, with extremely short segment involvement, patients may progress into adulthood, with bowel habits characterized by chronic constipation, and varying degrees of megacolon. Given that the pathophysiology involves a lack of caudal migration of neural crest cells to the distal gut, the end result is aganglionosis, and muscular hypertrophy of the distal rectum and anal canal. Histopathologically, this is characterized by the absence of ganglion cells, and hypertrophied nerve bundles. Functionally, this results in chronic nonrelaxation of the muscular wall of the bowel. Pelvic floor testing is the primary initial step to aid in diagnosis. The most common finding during physiologic testing is absence of the rectoanal inhibitory reflex . Other than as a result of surgical disruption, there are few other pathophysiologic processes that result in the absence of a RAIR, and in the proper clinical setting, it is considered a proxy for diagnosis [145]. In a patient who presents with a lifelong history of chronic constipation, especially in the face of endoscopic evidence of megacolon, RAIR testing provides the first key piece of evidence towards the diagnosis. Once the absence of a RAIR is confirmed, diagnosis is further made by histologic confirmation. Full-thickness biopsy of the rectal wall is required in order to perform microscopic assessment. Figure 3.16 shows the absence of a RAIR as compared to normal.
Low Anterior Resection Syndrome (LARS)
Low anterior resection syndrome (LARS) refers to the constellation of issues that to 80% of patients who undergo a low anterior resection will experience postoperatively. Symptoms include fecal urgency, frequency, bowel fragmentation, evacuation difficulty and incontinence to name a few. Most patients do regain relatively normal function by 6–12 months after surgery, however symptoms persisting after 1 year, are usually representative of permanent changes. The etiology of LARS is multifactorial, possibly due to sphincter injury, pudendal neuropathy, lumbar plexopathy, and in many cases radiation damage [146]. Diagnosis is primarily clinical, though scoring systems have been developed [74, 147,148,149]. Pelvic floor testing offers little in the way of predictive value in diagnosis and management since diagnosis is essentially based on symptoms, however after low anastomosis, there are significant decreases in compliance, as well as threshold volume and maximal tolerated volume [87]. To assess the effect of proctectomy on the RAIR, O’Riordain followed a cohort of 46 patients with pelvic floor testing after surgery. Pre-operatively, the RAIR was present in 93%. On the tenth post-operative day, it was only seen in 18%, and after 6–12 months only an additional 3% of patients had regained the reflex [150]. Thus, it is critical that patients are counseled preoperatively about the likelihood of functional changes after proctectomy. Treatment options include dietary management with bulking agents, antidiarrheal agents, daily enema therapy, biofeedback and sacral nerve stimulation [151].
Anismus
Anismus , otherwise known as nonrelaxation , or paradoxical contraction of the puborectalis during defecation is one of the more common etiologies for obstructive defecation syndrome [152]. In the normal state, the puborectalis muscle relaxes during defecation, straightening the anorectal angle, and allowing for unimpeded passage of stool. When this normal reflex is disordered, it is termed anismus. The cause of this dysfunction is unclear. It is felt to be multifactorial, involving both electro myogenic and psychological mechanisms [153,154,155,156,157]. Nonrelaxation of the puborectalis can be diagnosed in the office, both on physical examination as well as with anorectal manometry. Pressure over the puborectalis posteriorly during digital rectal exam while asking the patient to bear down and simulate defecation can reveal abnormal nonrelaxation. This finding can be more objectively confirmed during the “pushing” phase of manometric testing (Fig. 3.6) or EMG testing (Fig. 3.10). However, patient embarrassment during manometric testing may contribute to false positive results. Thus, diagnosis is made not only based on testing but also careful clinical history. The diagnosis is best confirmed by defecography, as the anorectal angle fails to open during defecation (Fig. 3.12). Once diagnosed, anismus may be treated with a variety of approaches including botulinum toxin injection, transanal electrostimulation and pelvic floor physiotherapy.
Rectocele, Sigmoidocele and Enterocele
Rectocele, sigmoidocele, and enterocele are clinical entities, which are often associated with constipation. Though clinical history can be suggestive of these disorders, defecography is the diagnostic modality of choice. Rectocele is an outpouching of the rectal wall during defecation. This is far more commonly found in females due to the relatively thin rectovaginal septum [158,159,160]. Rectoceles are classified anatomically depending on the location as low mid or high. Etiology is most commonly due to sphincter injury during childbirth, but may also be a result of chronic distention and straining with constipation [161]. The most common symptom of rectocele is a sense of incomplete evacuation, commonly associated with post-defecatory stool loss as the rectocele empties [162]. Rectocele is often a concomitant diagnosis with other pelvic organ prolapse including cystocele, enterocele and sigmoidocele, as well as uterine prolapse. Physical examination yields a prompt diagnosis. Rectoceles are graded relative to the degree of bulging into the vagina. Grade 1 rectocele is mild with little bulging, grade 2 rectocele is defined as bulging to the vaginal introitus, and grade 3 rectocele is bulging outside of the vaginal introitus. Dynamic studies such as defecography provide the most accurate and objective measure of rectocele (Fig. 3.13). A careful distinction must be made regarding the existence of a rectocele and its clinical importance. Studies have found that up to 80% of women have some degree of asymptomatic rectocele [163, 164]. Indication for surgical repair is based not only on symptomatology, but also predictive factors for successful repair. Karlbom found that the most predictive factor related to successful surgical treatment was resolution of obstructive symptoms with digital vaginal or perineal splinting (23/27 in the improved group vs. 3/7 in the non-improved group; p = 0.04) whereas a previous hysterectomy, large rectal area on cinedefecography and preoperative use of enemas, motor stimulants or several types of laxatives related to a poor result [165]. Other predictive factors that indicate prior success rates after surgical intervention include barium retention on defecography. Thus, patient selection is critical to successful surgical resolution. Good selection results in success rates of over 80% at 1-year follow-up [159, 164].
Sigmoidocele and enterocele refer to descent of the bowel into a deep rectovaginal sulcus. These are additional etiologies that can contribute to functional pelvic outlet obstruction. Previous hysterectomy is one of the risk factors, with incidences ranging from 6–25% [166]. Obliteration of the rectovaginal cul-de-sac is the typical approach for enterocele. Like with rectocele, the mere presence of enterocele or sigmoidocele may be an incidental finding on defecography. Only patients with clinically significant obstructive defecation merit consideration for surgical intervention. The pathophysiologic mechanism of obstruction may be multifactorial including collapse of the rectal wall due to extrinsic pressure by the sigmoidocele , as well as obstruction or stasis of the relatively entrapped bowel loop. This process is frequently accompanied by other manifestations of pelvic floor dysfunction including internal rectal prolapse, rectocele and anismus. Jorge and Wexner proposed classification based on the degree of descent of the lowest portion of the sigmoid. First-degree was defined as descent above the pubococcygeal line. Second-degree was descent below the pubococcygeal line and above the ischiococcygeal line, and third-degree was defined as descent below the ischiococcygeal line. In this study, the majority of third-degree sigmoidoceles were treated with sigmoid resection with or without rectopexy. The majority of first and second-degree sigmoidoceles were managed conservatively. In all patients who were treated surgically but in only one third of patients were treated surgically did post treatment symptoms improve [167]. Despite this success rate, surgery is rarely advised as although the anatomic deformity of the sigmoidocele is likely to be corrected, functional symptoms may persist or even be exacerbated.
Perineal Descent
Perineal descent is defined by excessive pelvic floor relaxation, resulting in descent of the perineum relative to the ischial tuberosities. It was first observed by Parks in 1966 in association with chronic constipation [106]. Subsequently it was found to be associated with other anorectal disorders such as incontinence and solitary rectal ulcer syndrome. Excessive perineal descent can force the anterior rectal wall to protrude into the anal canal, which may result in a sensation of incomplete evacuation, and pelvic floor weakening. This may feed forward, causing more straining, further stretching of pelvic floor musculature and further perineal descent. Parks postulated that such chronic straining of the pelvic floor anatomy could result in pudendal neuropathy [48]. Despite this logical association, no reliable correlation has been found between perineal descent and pudendal nerve terminal motor latency prolongation [168]. Perineal descent can be best measured by perineometry or defecography. Typically, during defecography a radio opaque marker is placed on the perineum and the pubococcygeal line is marked on static spot films. During the straining portion of defecography, the degree of descent can be measured directly.
Fecal Incontinence
The evolution of the utility of pelvic floor testing in the management of fecal incontinence has changed dramatically over the last 5 years. Prior to FDA approval in the United States, of sacral nerve stimulation in 2011, management of the majority of fecal incontinence was related to anatomic repair of sphincter injury. As a result, significant attention was paid to pelvic floor imaging and testing techniques. Although the gold standard, sphincter repair has poor long-term functional results. Thus, much attention was paid to identifying predictive factors for success or failure. Endoanal ultrasound is the most effective test for identifying the degree of sphincter injury. More recently, the use of three-dimensional ultrasound has enhanced our ability to image the sphincter and pelvic floor. Pudendal nerve testing showed conflicting results when relating to success after sphincteroplasty. Many investigators, including Barisic, Londono-Schimmer and Gilliland found significant differences in incontinence scores after sphincteroplasty in patients with and without pudendal neuropathy [169,170,171], however other, contemporaneous studies found no differences [172,173,174]. Since the advent of sacral nerve stimulation, the role of pelvic floor testing has been further weakened. Ratto and others have demonstrated equivalent efficacy of sacral nerve stimulation in the setting of sphincter injury, thus essentially obviating the need for preoperative endoanal ultrasound [175, 176]. Similarly, pelvic floor manometry has no predictive utility, likely since the overall success rate of sacral nerve stimulation is so high. In Hull’s publication on 5-year outcomes of sacral neuromodulation, multiple variables were examined to assess predictive value for success, including presence of sphincter defects and pudendal neuropathy as well as prior pelvic floor pathology, and no predictive values emerged [177]. Further studies have confirmed that manometry pressures, pudendal neuropathy, presence of a sphincter defect, or history of a prior sphincter repair do not predict the success of sacral nerve stimulation [178, 179]. Thus, in the setting of fecal incontinence , clinical judgment is more important than physiologic testing.
Summary
Pelvic floor testing can provide important objective information regarding the function of the pelvic floor. A careful understanding of the clinical significance of the information that can be gleaned aids the clinician in characterization, and in many cases, guides diagnosis and management. It is always important to interpret such data in the relevant clinical context, since only in selected cases, does such data provide clinically useful information.
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Paquette, I.M., Bleier, J.I.S. (2019). Anorectal Physiology Testing. In: Beck, D., Steele, S., Wexner, S. (eds) Fundamentals of Anorectal Surgery. Springer, Cham. https://doi.org/10.1007/978-3-319-65966-4_3
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