Abstract
The betaproteobacterial Azoarcus/“Aromatoleum”/Thauera cluster harbors a large variety of denitrifiers capable of anaerobic degradation of aromatic compounds and hydrocarbons. The application of genomics and proteomics (proteogenomics) in conjunction with targeted metabolite analysis has proven instrumental to the discovery of a wide variety of novel reactions and pathways, the reconstruction of complex catabolic networks, and first steps into understanding how the latter are tuned to adapt to environmental changes. Over the past two to three decades, major advances have been achieved along three major organism-/approach-specific directions: (i) biochemistry of novel enzymatic reactions with Thauera aromatica K172 and “Aromatoleum aromaticum” EbN1, (ii) physiology and proteogenomics with “A. aromaticum” EbN1, and (iii) molecular mechanisms of transcriptional regulation with Azoarcus sp. strain CIB.
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Rabus, R., Wilkes, H. (2019). Functional Genomics of Denitrifying Bacteria Degrading Hydrocarbons. In: Boll, M. (eds) Anaerobic Utilization of Hydrocarbons, Oils, and Lipids. Handbook of Hydrocarbon and Lipid Microbiology . Springer, Cham. https://doi.org/10.1007/978-3-319-33598-8_11-1
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