Abstract
A novel cell culture surface grafted with the temperature-responsive polymer poly(N-isopropylacrylamide) has been developed for controlled attachment and detachment of living cells through temperature changes. This temperature-responsive surface allows us to harvest confluent cells in the form of an intact monolayer cell sheet with deposited extra cellular matrices. Different from scaffold-based tissue engineering, cell sheet-based tissue engineering without the use of scaffolds provides another way for the development of tissue engineering. Taking advantage of the unique ability of cell sheets to generate three-dimensional (3D) tissues, we have also developed several methods to stack cell sheets and create functional tissues for therapy of a vast variety of diseases ranging from periodontitis and corneal disease to esophageal ulceration and heart failure. Moreover, we have created functional anisotropic tissues, thick vascularized tissues, and even organ-like systems by using cell sheet-based tissue engineering. In this chapter, we summarize development of temperature-responsive cell culture surfaces, cell sheet manipulation technologies, fabrication of thick tissues, and organ-like structures with cell sheets as well as applications of cell sheet-based tissue engineering.
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Gao, B., Shimizu, T., Okano, T. (2018). Cell Sorting, Culture, Preconditioning, and Modulation/Cell Aggregates: Sheets. In: Holnthoner, W., Banfi, A., Kirkpatrick, J., Redl, H. (eds) Vascularization for Tissue Engineering and Regenerative Medicine. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-21056-8_18-1
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