Abstract
Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.
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Acknowledgments
We acknowledge Bert Essenstam for excellent plant care. We are grateful to Yin Song and Yan Wang for their help with making the pictures.
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Zhang, Z., Thomma, B.P.H.J. (2014). Virus-Induced Gene Silencing and Agrobacterium tumefaciens-Mediated Transient Expression in Nicotiana tabacum . In: Birch, P., Jones, J., Bos, J. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 1127. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-986-4_14
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DOI: https://doi.org/10.1007/978-1-62703-986-4_14
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Publisher Name: Humana Press, Totowa, NJ
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