Abstract
Virus-induced gene silencing (VIGS) is a reverse genetics technique that is based on the RNA-mediated defense against viruses in plants. VIGS is a method of gene knockdown triggered by a replicating viral nucleic acid engineered to carry a host gene to be silenced. While there are a number of excellent VIGS vectors available for dicots, only a few are available for monocots. Here, we describe the detailed method of the use of a newly developed VIGS vector for rice, based on the rice-infecting Rice tungro bacilliform virus, a pararetrovirus with dsDNA genome. Using a method based on Agrobacterium-mediated injection of the VIGS construct at the meristematic region of young rice plants, silencing of target genes can be achieved and the silenced phenotype can be visualized in 3 weeks.
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Acknowledgments
AP and SS acknowledge the Research Fellowships from the University Grants Commission, New Delhi, and Council of Scientific and Industrial Research, New Delhi, respectively. This work was funded by the Department of Biotechnology, Government of India, New Delhi, Grant no. BT/AB/03/FG-I/2003 to ID.
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Purkayastha, A., Sharma, S., Dasgupta, I. (2013). Virus-Induced Gene Silencing for Rice Using Agroinoculation. In: Becker, A. (eds) Virus-Induced Gene Silencing. Methods in Molecular Biology, vol 975. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-278-0_3
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DOI: https://doi.org/10.1007/978-1-62703-278-0_3
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-278-0
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