Overview
- Features numerous techniques to study membrane proteins and membrane lipids as well as their interactions
- Includes detailed, lab-ready protocols and methodologies
- Contains expert tips and key implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 1033)
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About this book
Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
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Keywords
Table of contents (24 protocols)
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Investigation of Membrane Lipids
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Analysis of Structure and Topology of Membrane Proteins
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Studying Protein-Protein and Protein-Lipids Interactions within Membranes
Editors and Affiliations
Bibliographic Information
Book Title: Membrane Biogenesis
Book Subtitle: Methods and Protocols
Editors: Doron Rapaport, Johannes M. Herrmann
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-487-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-486-9Published: 01 September 2013
Softcover ISBN: 978-1-4939-6304-1Published: 23 August 2016
eBook ISBN: 978-1-62703-487-6Published: 31 August 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 404
Number of Illustrations: 32 b/w illustrations, 48 illustrations in colour
Topics: Cell Biology, Membrane Biology