Abstract
Many plant immune responses to biotic stress are mediated by the wound hormone jasmonate (JA). Functional and mechanistic studies of the JA signaling pathway often involve plant manipulations that elicit JA production and subsequent changes in gene expression in local and systemic tissues. Here, we describe a simple mechanical wounding procedure to effectively trigger JA responses in the Arabidopsis thaliana rosette. For comparison, we also present a plant–insect bioassay to elicit defense responses with the chewing insect Trichoplusia ni. This latter procedure can be used to determine the effect of JA-regulated defenses on growth and development of insect herbivores.
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Acknowledgments
This work was supported by the National Institutes of Health (grant R01GM57795) and the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, US Department of Energy (grant DE–FG02–91ER20021) for partial support of M.H. M.H. was also the recipient of a fellowship (HE 5949/1-1) from the German Research Foundation (DFG).
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Herde, M., Koo, A.J.K., Howe, G.A. (2013). Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory. In: Goossens, A., Pauwels, L. (eds) Jasmonate Signaling. Methods in Molecular Biology, vol 1011. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-414-2_5
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DOI: https://doi.org/10.1007/978-1-62703-414-2_5
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