Abstract
The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.
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Acknowledgment
This work was supported by grants from the German Research Foundation (GR 1517/12-1) and Fritz Thyssen Foundation (Az.10.10.2.122).
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Basler, M., Groettrup, M. (2013). Using Protease Inhibitors in Antigen Presentation Assays. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_3
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DOI: https://doi.org/10.1007/978-1-62703-218-6_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-217-9
Online ISBN: 978-1-62703-218-6
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