Abstract
Proteases generate peptides that bind to MHC class II molecules to interact with a wide diversity of CD4+ T cells. They are expressed in dedicated organelles: endosomes and lysosomes of professional antigen-presenting cells (pAPCs) such as B cells, macrophages, and dendritic cells. The identification of endosomal proteases which produce antigenic peptides is important for example for better vaccination and to prevent autoimmune diseases. Here, we describe a panel of techniques (in vitro digestion assays of protein with recombinant proteases or purified endosomes/lysosomes, T cell stimulation) to monitor the production of MHC class II ligands.
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Acknowledgments
This work was supported by INSERM (ANR 2010 MIDI 008 01), an ARC Ph.D. fellowship to S.M. and an Institut Curie Ph.D. fellowship to M.T.
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Tohmé, M., Maschalidi, S., Manoury, B. (2019). In Vitro Digestion with Proteases Producing MHC Class II Ligands. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_21
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DOI: https://doi.org/10.1007/978-1-4939-9450-2_21
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