Abstract
Yeast two-hybrid allows for investigation of interactions between two proteins in vivo. Yeast media can be supplemented with phycocyanobilin, allowing the formation of photoactive phytochromes, and the investigation of their light-regulated interactions. Three reporter assays are described; the histidine growth selection assay for the HIS3 reporter, and the filter lift and quantitative ONPG assays for the LacZ reporter. Design considerations for yeast two- and three-hybrid experiments are also covered.
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Sheerin, D.J. (2019). Investigation of Light-Regulated Protein–Protein Interactions Using Yeast Two-Hybrid Assays. In: Hiltbrunner, A. (eds) Phytochromes. Methods in Molecular Biology, vol 2026. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9612-4_1
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DOI: https://doi.org/10.1007/978-1-4939-9612-4_1
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