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Detecting Blue Light-Dependent Protein–Protein Interactions by LexA-Based Yeast Two-Hybrid Assay

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Plant Photomorphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2297))

Abstract

The LexA-based yeast two-hybrid system is one of the most powerful techniques used to detect blue light-dependent protein–protein interactions. In Arabidopsis, many protein–protein interactions in blue light signaling pathway were identified using this system. Here we present an easy and efficient method of the LexA-based yeast two-hybrid assay for testing protein–protein interactions in a blue light-dependent manner.

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Correspondence to Ling Li .

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Hao, X., Li, L. (2021). Detecting Blue Light-Dependent Protein–Protein Interactions by LexA-Based Yeast Two-Hybrid Assay. In: Yin, R., Li, L., Zuo, K. (eds) Plant Photomorphogenesis. Methods in Molecular Biology, vol 2297. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1370-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1370-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1369-6

  • Online ISBN: 978-1-0716-1370-2

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