Abstract
Differential scanning calorimetry (DSC) is a well-established technique for biomolecular stability studies. The technique is based on forced thermal denaturation of biomolecules in solution. Here we describe the use of DSC for characterization and optimization of stability of two proteins, a protein kinase and a mAb protein.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Johnson CM. Differential Scanning Calorimetry (DSC) Theory and Practice, Malvern Instruments White Paper. https://www.malvern.com/en/support/resource-center
Bruylants G, Wouters J, Michaux C (2005) Differential scanning calorimetry in life science: thermodynamics, stability, molecular recognition and application in drug design. Curr Med Chem 12:2011–2020
Jelesarov I, Bosshard HR (1999) Isothermal titration calorimetry and differential scanning calorimetry as complementary tools to investigate the energetics of biomolecular recognition. J Mol Recognit 12:3–18
Choi MH, Prenner EJ (2011) Differential scanning calorimetry: An invaluable tool for a detailed thermodynamic characterization of macromolecules and their interactions. J Pharm Bioallied Sci 3:39–59
Johnson CM (2013) Differential scanning calorimetry as a tool for protein folding and stability. Arch Biochem Biophys 531:100–109
Plotnikov V, Rochalski A, Brandts M, Brandts JF, Williston S, Frasca V, Lin LN (2002) An autosampling differential scanning calorimeter instrument for studying molecular interactions. Assay Drug Dev Technol 1:83–90
Demarest SJ, Frasca V (2015) Differential scanning calorimetry in the biopharmaceutical sciences. In: Houde DJ, Berkowitz SA (eds) Biophysical characterization of proteins in developing biopharmaceuticals. Elsevier, Amsterdam
Morar-Mitrica S, Nesta D, Crofts G (2013) Differential scanning calorimetry (DSC) for biopharmaceutical development: old concepts, new applications. Biopharm Asia 2:46–55
Makhatadze G (1998) Measuring protein thermostability by differential scanning calorimetry. Curr Protoc Protein Sci 7.9.1–7.9.14
Gabrielson J, Weiss IV (2015) Technical decision-making with higher order structure data: starting a new dialogue. J Pharm Sci 104:1240–1245
Arthur KK, Dinh N, Gabrielson JP (2015) Technical decision making with higher order structure data: utilization of differential scanning calorimetry to elucidate critical protein structural changes resulting from oxidation. J Pharm Sci 104:1548–1554
Ionescu RM, Vlasak J, Price C, Kirchmeier M (2008) Contribution of variable domains to the stability of humanized IgG1 monoclonal antibodies. J Pharm Sci 97:1414–1426
O’Brien R. Differential scanning calorimetry (DSC) for biopharmaceutical development. Recorded Malvern webinar. https://www.malvern.com/en/support/resource-center
Katherine B. The power of heat: digging deeper with differential scanning calorimetry to study key protein characteristics. Recorded Malvern webinar. https://www.malvern.com/en/support/resource-center
Markova N. Value of DSC in characterization and optimization of protein stability as compared to other thermal stability assays. Recorded Malvern webinar. https://www.malvern.com/en/support/resource-center
Acknowledgments
The Sprint Bioscience AB (Huddinge, Sweden) is acknowledged for providing constructs of protein kinase.
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bowers, K., Markova, N. (2019). Value of DSC in Characterization and Optimization of Protein Stability. In: Ennifar, E. (eds) Microcalorimetry of Biological Molecules. Methods in Molecular Biology, vol 1964. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9179-2_3
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9179-2_3
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9178-5
Online ISBN: 978-1-4939-9179-2
eBook Packages: Springer Protocols