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Differential Scanning Calorimetry of Protein–Lipid Interactions

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Lipid-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 974))

Abstract

Differential scanning calorimetry (DSC) is a highly sensitive non-perturbing technique for measuring the thermodynamic properties of thermally induced transitions. This technique is particularly useful for the characterization of lipid/protein interactions. This chapter presents an introduction to DSC instrumentation, basic theory, and methods and describes DSC applications for characterizing protein effects on model lipid membranes. Examples of the use of DSC for the evaluation of protein effects on modulation of membrane domains and membrane stability are given.

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Acknowledgments

This work was supported by the Ministerio de Ciencia e Innovación (SAF2009-07810) and Instituto de Salud Carlos III (cibeRES-CB06/06/0002).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology I, Faculty of Biology, CIBER de Enfermedades Respiratorias, Madrid, Spain

    Olga Cañadas

  2. Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University of Madrid, Madrid, Spain

    Cristina Casals

Authors
  1. Olga Cañadas
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  2. Cristina Casals
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Corresponding author

Correspondence to Cristina Casals .

Editor information

Editors and Affiliations

  1. Institut für Biologie, Abteilung Biophysik, Universität Kassel, Heinrich-Plett-Straße 40, Kassel, 34132, Germany

    Jörg H. Kleinschmidt

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Cañadas, O., Casals, C. (2013). Differential Scanning Calorimetry of Protein–Lipid Interactions. In: Kleinschmidt, J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 974. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-275-9_4

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  • DOI: https://doi.org/10.1007/978-1-62703-275-9_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-274-2

  • Online ISBN: 978-1-62703-275-9

  • eBook Packages: Springer Protocols

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