Abstract
Shoot phototropism benefits growth and metabolism in land plants by enabling them to position their photosynthetic organs in favorable light conditions. Nonvascular land plants, like the ancestors of modern mosses, are believed to have been among the first plants to occupy the land. To understand the evolutional history of shoot phototropism in land plants, we have established a system for experimentally studying phototropism in gametophores of the moss Physcomitrella patens. Here we will describe the key points in our system, including obtaining etiolated gametophores, the light sources used for inducing bending, and the methods for evaluation of phototropic responses.
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References
Bao L, Yamamoto KT, Fujita T (2015) Phototropism in gametophytic shoots of the moss Physcomitrella patens. Plant Signal Behav 10:e1010900. https://doi.org/10.1080/15592324.2015.1010900
Briggs WR (2014) Phototropism: some history, some puzzles, and a look ahead. Plant Physiol 164:13–23. https://doi.org/10.1104/pp.113.230573
Esch H, Hartmann E, Cove D, Wada M, Lamparter T (1999) Phytochrome-controlled phototropism of protonemata of the moss Ceratodon purpureus: physiology of the wild type and class 2 ptr-mutants. Planta 209:290–298. https://doi.org/10.1007/s004250050635
Fujita T, Sakaguchi H, Hiwatashi Y, Wagstaff SJ, Ito M, Deguchi H, Sato T, Hasebe M (2008) Convergent evolution of shoots in land plants: lack of auxin polar transport in moss shoots. Evol Dev 10:176–186
Tomescu AM, Wyatt SE, Hasebe M, Rothwell GW (2014) Early evolution of the vascular plant body plan—the missing mechanisms. Curr Opin Plant Biol 17:126–136. https://doi.org/10.1016/j.pbi.2013.11.016
Ito K, Ren J, Fujita T (2014) Conserved function of rho-related Rop/RAC GTPase signaling in regulation of cell polarity in Physcomitrella patens. Gene 544:241–247. https://doi.org/10.1016/j.gene.2014.04.057
Acknowledgment
The authors would like to thank Dr. Mark Running of the University of Louisville for critical reading and revising the manuscript.
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Bao, L., Yamamoto, K.T., Fujita, T. (2019). An Experimental System for Examining Phototropic Response of Gametophytic Shoots in the Moss Physcomitrella patens. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_5
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DOI: https://doi.org/10.1007/978-1-4939-9015-3_5
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