Abstract
Microscopic investigations of biological objects are an integral part in plant research and most fields of life sciences. They allow the description of morphological, histological, and structural aspects of individual cells or tissues. Based on various cell biological tools and methods it is possible to characterize different plant genotypes or study their adaptation to changing environmental conditions. In combination with antibodies raised against specific antigens and epitopes light and electron microscopy enable investigation of the function of single genes/proteins in plant growth and development or their role related to abiotic or biotic stresses.
Here, we describe sample preparation of barley roots for cell biological investigations using light and electron microscopy, to characterize morphological, structural, and functional aspects on root sections and the root surface.
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References
Barr M, Fahy A, Martens J, Jardine AP, Ward DJ, Ellis J, Allison W, Dastoor PC (2016) Unlocking new contrast in a scanning helium microscope. Nat Commun 7:10189. https://doi.org/10.1038/ncomms10189
Henning W, Andersen JB, Højgaard L, Greisen G, Law I, Thorseth A, Christensen AN (2015) A new self-made digital slide scanner and microscope for imaging and quantification of fluorescent microspheres. J Biomed Graph Comput 5:33
Haller E, Hudson J, Kelly A, Cotta DA, Peaudecerf B, Bruce GD, Kuhr S (2015) Single-atom imaging of fermions in a quantum-gas microscope. Nature Phys 11:738–742
Liu CS, Lin YC, Hu PH (2013) Design and characterization of precise laser-based autofocusing microscope with reduced geometrical fluctuations. Microsyst Technol 19:1717–1724
Foissner W (2014) An update of ‘basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa’. Int J Syst Evol Microbiol 64:271–292
King GA, Gross P, Bockelmann U, Modesti M, Wuite GJ, Peterman EJ (2013) Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching using fluorescence microscopy. Proc Natl Acad Sci U S A 110:3859–3864
Li H, Pu Y, Kumar R, Ragauskas AJ, Wyman CE (2014) Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms. Biotechnol Bioeng 111:485–492
Marzec M, Melzer M, Szarejko I (2013) Asymmetric growth of root epidermal cells is related to the differentiation of root hair cells in Hordeum vulgare (L.). J Exp Bot 64:5145–5155
Ruzin SE (1999) Plant microtechnique and microscopy, vol 198. Oxford University Press, New York
Marzec M, Szarejko I, Melzer M (2015) Arabinogalactan proteins are involved in root hair development in barley. J Exp Bot 66:1245–1257
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Marzec, M., Melzer, M. (2019). Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy. In: Harwood, W. (eds) Barley. Methods in Molecular Biology, vol 1900. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8944-7_10
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DOI: https://doi.org/10.1007/978-1-4939-8944-7_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8942-3
Online ISBN: 978-1-4939-8944-7
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