Abstract
Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.
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Acknowledgment
The work presented in this chapter was supported by Grant W81XWH-15-1-0257 from the US Department of Defense. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Department of Defense.
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Zhuang, L., Zhan, X., Bi, Y., Ji, B. (2019). Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_25
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DOI: https://doi.org/10.1007/978-1-4939-8879-2_25
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