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Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous

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Microbial Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1852))

Abstract

Xanthophyllomyces dendrorhous, a heterobasidiomycetous yeast that represents the teleomorphic state of Phaffia rhodozyma, is used as a natural source of several carotenoids, such as the xanthophyll astaxanthin. Here, we describe the culture procedure for the production of carotenoids in X. dendrorhous and a simple and rapid analytical method for the optimized extraction and HPLC determination of intracellular β-carotene, astaxanthin, canthaxanthin, and zeaxanthin.

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Author information

Authors and Affiliations

  1. INBIOTEC, Instituto de Biotecnología de León, León, Spain

    Carlos García-Estrada, Katarina Kosalková & Isabel-Clara Sánchez-Orejas

  2. Área de Toxicología, Departamento de Ciencias Biomédicas, Universidad de León, León, Spain

    Carlos García-Estrada

Authors
  1. Carlos García-Estrada
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  2. Katarina Kosalková
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  3. Isabel-Clara Sánchez-Orejas
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Corresponding author

Correspondence to Carlos García-Estrada .

Editor information

Editors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

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García-Estrada, C., Kosalková, K., Sánchez-Orejas, IC. (2018). Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8742-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8741-2

  • Online ISBN: 978-1-4939-8742-9

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