Abstract
Like many biological processes, oocyte development depends on careful orchestration of protein localization. Optogenetic approaches have the potential to manipulate this dynamic system with spatial and temporal precision and molecular specificity. This chapter describes the use of a photocaged chemical inducer of dimerization to control localization of genetically tagged proteins with light. As an example, we recruit a fluorescently tagged protein to one spindle pole in metaphase.
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Akera, T., Chenoweth, D.M., Lampson, M.A. (2018). Optogenetic Manipulation of Mouse Oocytes. In: Verlhac, MH., Terret, ME. (eds) Mouse Oocyte Development. Methods in Molecular Biology, vol 1818. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8603-3_13
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DOI: https://doi.org/10.1007/978-1-4939-8603-3_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8602-6
Online ISBN: 978-1-4939-8603-3
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