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Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation

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Investigations of Early Nutrition Effects on Long-Term Health

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1735))

Abstract

Multiplex immunoassays are used for rapid profiling of biomarker proteins and small molecules in biological fluids. The advantages over single immunoassays include lower sample consumption, cost, and labor. This chapter details a protocol to develop a 5-plex assay for glucagon-like peptide 1, growth hormone, insulin, leptin, and thyroid-stimulating hormone on the Luminex® platform. The results of the analysis of insulin in normal control subjects are given due to the important role of this hormone in nutritional programming diseases.

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Author information

Authors and Affiliations

  1. Ampersand Biosciences, Saranac Lake, NY, USA

    Laurie Stephen

  2. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil

    Paul C. Guest

Authors
  1. Laurie Stephen
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  2. Paul C. Guest
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Corresponding author

Correspondence to Paul C. Guest .

Editor information

Editors and Affiliations

  1. Debden, Essex, United Kingdom

    Paul C. Guest

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Stephen, L., Guest, P.C. (2018). Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation. In: Guest, P. (eds) Investigations of Early Nutrition Effects on Long-Term Health. Methods in Molecular Biology, vol 1735. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7614-0_32

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  • DOI: https://doi.org/10.1007/978-1-4939-7614-0_32

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7613-3

  • Online ISBN: 978-1-4939-7614-0

  • eBook Packages: Springer Protocols

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