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Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies

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Neurotrophic Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1727))

Abstract

The blood-brain barrier (BBB) is essential to maintain the proper microenvironment for brain function. Although formed by different cell types, the endothelial cells (ECs) of the brain microvessels provide the BBB with its selective permeability. To study the BBB in vitro, EC lines as well as primary isolated ECs have been used. In this chapter, we will provide a detailed protocol on how to isolate and culture primary brain microvascular endothelial cells from different species for use as in vitro models of the BBB. When performed properly, this protocol will allow one to obtain a pure culture of brain microvascular endothelial cells with which to analyze the effects of therapeutic and toxic agents on BBB functions.

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Acknowledgments

This work was supported in part by an appointment (H.R.-H.) to the Research Participation Program at the National Center for Toxicological Research administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the US Department of Energy and the US Food and Drug Administration.

Disclaimer The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the US Food and Drug Administration. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

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Authors and Affiliations

  1. Division of Neurotoxicology, HFT-132, National Center for Toxicological Research/USFDA, Jefferson, AR, USA

    Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule & Syed F. Ali

Authors
  1. Hector Rosas-Hernandez
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  2. Elvis Cuevas
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  3. Susan M. Lantz
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  4. Merle G. Paule
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  5. Syed F. Ali
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Corresponding author

Correspondence to Syed F. Ali .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stephen D. Skaper

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Rosas-Hernandez, H., Cuevas, E., Lantz, S.M., Paule, M.G., Ali, S.F. (2018). Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7571-6_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7570-9

  • Online ISBN: 978-1-4939-7571-6

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